scholarly journals Phytochemical Profiling of Leaf, Stem, and Tuber Parts of Solena amplexicaulis (Lam.) Gandhi Using GC-MS

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Karthika Krishnamoorthy ◽  
Paulsamy Subramaniam
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Plant Extracts ◽  
Mass Spectra ◽  
Molecular Formula ◽  
Good Source ◽  
Methanolic Extracts ◽  
Solena Amplexicaulis ◽  
Structure Category ◽  
Molecular Weight Peak

Objective. To explore the possible bioactive compounds in the methanolic extracts of leaf, stem, and tuber parts of the medicinal climber, Solena amplexicaulis, using GC-MS. Methods. GC-MS analysis of the plant extracts were performed by using GC-MS-5975C [Agilent] and mass spectra of the compounds found in the extract was matched with the data in the library of National Institute of Standards and Technology (NIST). Results. Thirty-five compounds were determined to be present in the parts studied. The active principles with their retention time, molecular formula, molecular weight, peak area, structure, category of the compounds, and activities were predicted. The most prevailing compounds were phytol (38.24%) in leaf, 4-(4-ethoxyphenyl) but-3-en-2-one (56.90%) in stem, and 9,17-octadecadienal, (Z)- (21.77%) in tuber. Conclusion. This study revealed that the species S. amplexicaulis is a good source of many bioactive compounds like terpenes, triazines, esters, alkanes, alcohols, hydrocarbons, aldehydes, amides, and so forth. That justifies the traditional usage of this species.

scholarly journals Gas chromatography-mass- spectroscopy analysis of bioactive compounds from Streptomyces spp. isolated from Tigris river sediments in Baghdad city

2020 ◽  
Vol 14 (1) ◽  
pp. 63-71
Author(s):  
Talib S. Al-rubaye ◽  
Mohsen H. Risan ◽  
Dalal Al-rubaye
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Mass Spectroscopy ◽  
River Sediments ◽  
Antibacterial Activities ◽  
Hexanoic Acid ◽  
Molecular Formula ◽  
Streptomyces Spp ◽  
Secondary Screening ◽  
Amine Compounds

Background:The Streptomyces are considered the most important bacterial source for bioactive compounds production including natural antibiotics. Objective: This study focused on analysis of these products to characterize the most active substances which may contain new antibiotics. Materials and methods: Samples with the highest antibacterial activities (21, M5, N- and D-) were chosen from a previous study after secondary screening for the intracellular (biomass) extract which showed more antagonism efficiency than that observed in extracellular crude extract.Gas chromatography – mass spectroscopy (GC-MS) was preformed to detect the structure of the compounds in intracellular crude extracts in these isolates. Results: The GC-MS analysis showed a total of 49 peaks observed in 4 isolates, isolate M5=14 peaks, isolate D=11peaks, isolate N= 20 peaks and isolate 21= 4peaks. Isolate D-, which showed the highest zone of inhibition in secondary screening than that in other isolates, is associated with the most prevalence active compounds like the Decane derivatives, in addition to Triadimenol; Azetidine, 1-(1,1-dimethylethyl)-3-methyl; Hexanoic acid, 2-ethyl-, 2-ethylhexyl ester and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane. While isolate 21, has less peaks in comparing with the other samples, with great occurrence in components: 1-Dimethylaminohexane with molecular formula C8H19N and molecular weight 129 and Propamocarb with molecular formula C9H20N2O2 and molecular weight 188, in addition to many volatile organic compounds. The greatest components of isolate M5 were Triadimenol and 3,3,7,7-Tetramethyl-1,5-diazabicyclo(3.3.0)octane, in addition to the presence of Decane derivatives; amine compounds and Vitamin E. Isolate N- showed a great occurrence with components Triadimenol and Azetidine, 1-(1,1-dimethylethyl)-3-methyl-with a molecular formula C8H17N and with a molecular weight 127; also the presence of an important component Hexanoic acid, 2-methyl- with the molecular formula C7H14O2 and with molecular weight 103 which has been considered as an essential component of muramycin antibiotic; compounds which contain  Benzene ring. Conclusion: The most prominent compounds detected in the selected isolates by using GC-MS technique were Decane derivatives and Triadimenol.

scholarly journals GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS IN ETHANOL LEAVES EXTRACT OF SPHENOCENTRUM JOLLYANUM AND THEIR BIOLOGICAL ACTIVITIES.

2022 ◽  
Vol 06 (01) ◽  
Author(s):  
Emmanuel Uka1,
Keyword(s):  
Molecular Weight ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Octadecenoic Acid ◽  
Acid Ethyl Ester ◽  
Molecular Formula ◽  
Hexadecanoic Acid ◽  
Total Extract ◽  
Medicinal Uses ◽  
Ms Analysis

Sphenocentrum jollyanum is a plant genus of the family Menispermaceae. It has high medicinal importance as it is used traditionally to treat various diseases such as jaundice, breast engorgement related to the menstrual cycle, tumour, fibroids and improve the health of people. The present investigation was carried out to analyze the bioactive compounds present in ethanol crude extract of Sphenocentrum jollyanum leaves using GC-MS analysis. GC-MS analysis of ethanol extract Sphenocentrum jollyanum was done using a 7890A GC system (Agilent Technologies), coupled with 5977B MSD (Agilent Technologies) while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. A total of 45 bioactive compounds representing 99.98% of the total extract based on the retention time, peak area, molecular formula, molecular weight, and biological activities were identified by GC-MS which ranges from high molecular weight to low molecular weight compounds. The major compounds identified with their peak area percentages were 2,4-Di-tertbutylphenol, (21.05%), Z-8-Methyl-9-tetradecenoic acid (19.12), Hexadecanoic acid, ethyl ester (7.86%), Diisooctyl phthalate (7.13%), Phytol, Oleic Acid (7.03), 6,9,12- Octadecatrien-1-ol (6.65%), 3-Eicosene, (E)-(4.63%), 2-Methyl-Z, Z-3,13-octadecadienol (4.24%), n- Hexadecanoic acid (4.09%), trans-13-Octadecenoic acid (3.81%), Cyclohexene, 4-(4-ethylcyclohexyl) -1- pentyl- (3.74%), Dibutyl phthalate (3.20%), and 9-Oxabicyclo (6.1.0) nonane, cis-(3.18%). The presence of these major phytoconstituents in the leaf extract provides various biological activities including antifungal, antibacterial, antioxidant, anti-inflammatory, and anti-tumour which supports the ethno-medicinal uses of the plant in curing diseases. We recommend

Analysis of Bioactive Compounds of Methanolic Leaves extract of Mentha pulegium Using Gas Chromatography-Mass Spectrometry (GC-MS) Technique

2017 ◽  
Vol 8 (04) ◽  
Author(s):  
Israa Adnan Ibraheam ◽  
Mohammed Yahya Hadi ◽  
Imad Hadi Hameed
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Mentha Pulegium

The objective of this study was analysis of the secondary metabolite products. Bioactives are chemical compounds often referred to as secondary metabolites. Sixteenth bioactive compounds were identified in the methanolic extract of Mentha pulegium. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Mentha pulegium revealed the existence of the Erythritol , Cyclohexanone , 3-methyl-,(R)- , 2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one , 1-Oxaspiro[2.5]octan-4-one ,2,2,6-trimethyl-, cis- , Terpinyl formate , Acetamide , N-methyl-N-[4-(3-hydroxypyrrolidinyl)-2-butynyl]- , Pulegone , 2-Oxabicyclo[3.3.0]oct-7-en-3-one , 7-(1-hydroxypentyl)- , 2(3H)-Naphthalenone ,4,4a,5,6,7,8-hexahydro-1-methoxy- , 2-Cyclopenten-1-one , 2-(2-butenyl)-4-hydroxy-3-methyl-,(Z)- , (5β)Pregnane-3,20β-diol 14α,18α-[4-methyl-3-oxo-(1-oxa-4- , 2-(4-(But-2-yl)phenyl) propnoic acid , Nootkaton-11,12-epoxide , 2-Heptanone , 6-methyl-6-[3-methyl-3-(1-methylethenyl)-1-cyclo , Cholestan-3-ol , 2-methylene-, (3β,5α)- , 1-Heptatriacotanol and Digitoxin.

In vitro antibacterial and antibiotic-potentiation activities of five edible plant extracts and mode of action against several MDR Gram-negative phenotypes

2020 ◽  
Vol 4 (1) ◽  
pp. 1-14
Author(s):  
Carine M.N. Ngaffo ◽  
Simplice B. Tankeo ◽  
Michel-Gael F. Guefack ◽  
Brice E. N. Wamba ◽  
Paul Nayim ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Plant Extracts ◽  
Theobroma Cacao ◽  
Efflux Pumps ◽  
Phoenix Dactylifera ◽  
Food Plants ◽  
Proton Pumps ◽  
Gram Negative ◽  
E Coli

Abstract Background: Bacterial infections involving the multidrug resistant (MDR) strains are among the top leading causes of death throughout the world. Healthcare system across the globe has been suffering from an extra-ordinary burden in terms of looking for the new and more potent antimicrobial compounds. The aim of the present study was to determine the antibacterial activity of some Cameroonian edible plants (Garcinia lucida bark, Phoenix dactylifera pericarps, Theobroma cacao pod, Solanum macrocarpon leaves and Termitomyces titanicus whole plant) and their antibiotics-potentiation effects against some MDR Gram-negative bacteria phenotypes expressing efflux pumps (Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Pseudomonas aeruginosa and Providencia stuartii strains). Methods: The antibacterial activities of plant extract alone and in combination with usual antibiotics were carried out using the micro-dilution method. The effects of the most active plant extract (Garcinia lucida bark) on H+-ATPase-mediated proton pumps and on bacterial growth kinetic were performed using experimental protocols, while qualitative reference methods were used to highligh the major groups of secondary metabolites present in the extracts. Results: Qualitative phytochemical screening of plant extracts indicated that all analysed secondary metabolites were present in Theobroma cacao and Termitomyces titanicus while one (saponins) of them was absent in Garcinia lucida and Solanum macrocarpon. Only three of them (polyphenols, flavonoids and saponins) were detected in Phoenix dactylifera. Antibacterial essays showed that G. lucida was the most active plant as it inhibited the growth of all studied bacteria with strong activity (MIC<100 µg/mL) against E. coli ATCC8739, significant activity (100≤MIC≤512 µg/mL) against 80% of bacteria and moderate activity (512<MIC≤2048 µg/mL) against E. coli AG100A and E. aerogenes (EA289 and CM64). It was followed by T. cacao and S. macrocarpon extracts which exhibited an antibacterial potential against 95% and 80% of bacterial strains, respectively. These three extracts exhibited a bactericidal effect on a few bacteria. Extracts from T. titanicus and P. dactylifera were less active as they moderately (512<MIC≤2048 µg/mL) inhibited the growth of 35% and 10% of bacteria. All extracts selectively potentiated the activities of all antibiotics with improvement activity factors (IAF) ranging from 2 to 256. G. lucida, T. cacao and S. macrocarpon potentiated the activities of 100%, 89% and 67% of antibiotics respectively against more than 70%, suggesting that they contain bioactive compounds which could be considered as efflux pumps inhibitors. Whereas T. titanicus and P. dactylifera improved the activities of almost 40% and 20% of antibiotics, respectively. This increase of activities also characterizes synergistic effects between antibiotics and these bioactive compounds. G. lucida extract at all tested concentrations, strongly inhibited the growth of bacterial strain E. coli ATCC8739 and exhibited an inhibitory effect on this bacterial H+-ATPase-mediated proton pumps increasing the pH of the medium. Conclusion: The overall results indicated that food plants among which G. lucida, T. cacao and S. macrocarpon could have a benefit interest in combatting resistant types of bacteria. Keywords: Food plants; infectious diseases; MDR bacteria; efflux pumps; antibiotics; secondary metabolites.

scholarly journals Cold Steeping Infusion, a Novel Lectin Extraction Technique for the Isolation, Purification and Partial Characterization of Lectins from the Green Venezuelan Marine Alga Caulerpa serrulata

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Pablo Djabayan-Djibeyan ◽  
Brian Carpenter ◽  
Gerardo Medina-Ramírez ◽  
Felix Andueza-Leal ◽  
Andrés León-Leal ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Red Blood Cells ◽  
Ammonium Sulfate ◽  
Bioactive Compounds ◽  
Blood Cells ◽  
Marine Alga ◽  
Continuous Production ◽  
Isotonic Saline ◽  
Size Exclusion ◽  
Extraction Technique

A lectin from the green Venezuelan marine alga Caulerpa serrulata was extracted with phosphate buffered saline (PBS) using cold steeping infusion (CSI) and by grinding with liquid nitrogen (GLN). The proteins were precipitated using solid ammonium sulfate. Both the crude extracts and ammonium sulfate precipitated proteins were tested for hemagglutinins using native and papain-treated human red blood cell suspensions in isotonic saline solution. Purification of lectins was achieved using affinity chromatography-sugar-epoxy-sepharose 6B and molecular weight was assessed by size exclusion chromatography using Bio-gel® P-100 and SDS-PAGE with 2-mercaptoethanol. IEF-urea 8M was also evaluated. Using CSI it was shown that the marine alga released hemagglutinating compounds into the solutions; the same hemagglutinating compounds were also obtained by GLN. Ammonium sulfate precipitated proteins exhibited agglutinating activity against native and papain-treated human red blood cells. Temperature and EDTA were shown to affect dramatically the lectin activity towards red blood cells. A lectin was purified efficiently and the molecular weight calculated as approximately 78,000 Daltons. The CSI technique demonstrated that the alga could be returned to an active metabolic state by immersion in a simple buffer after having been kept dormant by freezing at −20°C for long periods. It was also shown that the alga was releasing bioactive compounds into the solutions and, therefore, this procedure is being suggested as a good, gentle, non-disruptive extraction technique and we postulate CSI as a possible bioreactor for the continuous production of bioactive compounds from green marine algae.

Interaction of high molecular weight kininogen binding proteins on endothelial cells

2004 ◽  
Vol 91 (01) ◽  
pp. 61-70 ◽  
Author(s):  
Baby Tholanikunnel ◽  
Berhane Ghebrehiwet ◽  
Allen Kaplan ◽  
Kusumam Joseph
Keyword(s):  
Molecular Weight ◽  
Endothelial Cell ◽  
High Molecular Weight ◽  
Gel Filtration ◽  
Surface Proteins ◽  
Factor Xii ◽  
High Molecular Weight Kininogen ◽  
Dot Blot ◽  
Cell Plasma ◽  
Molecular Weight Peak

SummaryCell surface proteins reported to participate in the binding and activation of the plasma kinin-forming cascade includes gC1qR, cytokeratin 1 and u-PAR. Each of these proteins binds high molecular weight kininogen (HK) as well as Factor XII. The studies on the interaction of these proteins, using dot-blot analysis, revealed that cytokeratin 1 binds to both gC1qR and u-PAR while gC1qR and u-PAR do not bind to each other. The binding properties of these proteins were further analyzed by gel filtration. When biotinylated cytokeratin 1 was incubated with either gC1qR or u-PAR and gel filtered, a new, higher molecular weight peak containing biotin was observed indicating complex formation. The protein shift was also similar to the biotin shift. Further, immunoprecipitation of solubilized endothelial cell plasma membrane proteins with anti-gC1qR recovered both gC1qR and cytokeratin 1, but not u-PAR. Immunoprecipitation with anti-u-PAR recovered only u-PAR and cytokeratin 1. By competitive ELISA, gC1qR inhibits u-PAR from binding to cytokeratin 1; u-PAR inhibits gC1qR binding to a lesser extent and requires a 10-fold molar excess. Our data suggest that formation of HK (and Factor XII) binding sites along endothelial cell membranes consists of bimolecular complexes of gC1qR-cytokeratin 1 and u-PAR-cytokeratin 1, with gC1qR binding being favored.

Synthesis of Cyclic Polysulfides and their Properties as Curing Agents

2004 ◽  
Vol 77 (2) ◽  
pp. 380-390
Author(s):  
Wonmun Choi ◽  
Tomoyuki Matsumura
Keyword(s):  
Mechanical Properties ◽  
Molecular Weight ◽  
Tensile Strength ◽  
High Molecular Weight ◽  
Mass Spectra ◽  
Elongation At Break ◽  
Molecular Weights ◽  
Aging Properties ◽  
Curing Agents ◽  
Polysulfide Polymer

Abstract The reactions of dichloroalkanes and sodium tetra-sulfide (Na2S4) were carried out in a mixture of water and toluene to produce corresponding cyclic polysulfides and polysulfide polymer. The low molecular weights of cyclic sulfides were obtained by the reaction at 90 °C, while the high molecular weight of polysulfide polymer was obtained by the reaction at 50 °C. GPC chromatograms and Mass spectra revealed that the structures of cyclic polysulfide were 1:1, 2:2, and 3:3 adducts of dichloroalkane and sodium tetra-sulfide. The mechanical properties of vulcanized NR at 148 °C with cyclic sulfides were similar to that with sulfur. However, both tensile strength and elongation at break of vulcanized NR at 170 °C with cyclic sulfides are much higher than that with sulfur. The aging properties of vulcanized NR at 148 °C or 170 °C with cyclic polysulfides indicate better stability.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential oils of 14 known Ficus species – A Concise Review

2021 ◽  
Vol 12 (6) ◽  
pp. 8003-8034
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Volatile Oils ◽  
Good Source ◽  
Concise Review ◽  
Living Organisms

Compounds useful for drugs, cosmetics, and food have been obtained directly or indirectly from living organisms over the years. However, there has been a renewed interest in getting useful compounds from living organisms, especially plants. Essential oils, interchangeably called volatile oils, are bioactive compounds found in minute quantities in some plants. Essential or volatile oils have been known for years to find usefulness in foods, drugs (antimicrobial, antifungal), and cosmetics. This review attempts to summarize information on the essential oil from Ficus species concerning their morphology, pharmacology, bioactivity, and application. This was achieved by gathering information on essential oils from different Ficus species. Essential oils from Ficus species are a good source of bioactive compounds for use in drug, food, and cosmetic industries. It is worthy to note that Nigerian Figs were characterized by the high presence of phytol and 6,10,14-trimethyl-2-pentadecanone, and these compounds are, therefore, seen as markers. Furthermore, this review presents numerous insights on how to best harness the different potentials of the essential oils and possibilities to be examined.

IN VITRO STUDIES OF SOME EDIBLE SPICES ON PANCREATIC LIPASE INHIBITORY ACTIVITY

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (02) ◽  
pp. 62-68
Author(s):  
S Mhatre ◽  
◽  
A. Bhagit ◽  
R. P Yadav
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Syzygium Aromaticum ◽  
High Potency ◽  
Good Source ◽  
Zanthoxylum Armatum ◽  
Methanolic Extracts ◽  
Cinnamomum Tamala

Pancreatic lipase inhibitory effect of some edible spices in light of percent inhibition, efficacy, reversibility/ irreversibility and effect of pH on inhibition is presented here. Lipase inhibitory activities of methanolic extracts of eighteen spices were evaluated. Extracts of Zanthoxylum armatum, Cinnamomum tamala, Syzygium aromaticum and Myristica fragrans were considered to be of high potency in synthetic substrate assay. Only Syzygium aromaticum showed high potency in natural substrate based lipase assay. Zanthoxylum armatum extract displayed lowest IC50 of 9.0 μg/mL. On dialysis, all extracts lost their lipase inhibitory activity indicating reversible nature of inhibition. pH significantly affected the performance of spice extracts during inhibition of pancreatic lipase. Most of the extracts lost their pancreatic lipase inhibitory activity at pH 3.0 with the exception of Brassica nigra and Cinnamomum tamala. Results showed spice are good source of pancreatic lipase inhibitor and its potential as drug for obesity can be explored by addressing various issues.

scholarly journals MMHub, a database for the mulberry metabolome

Database ◽  
2020 ◽  
Vol 2020 ◽  
Author(s):  
Dong Li ◽  
Bi Ma ◽  
Xiaofei Xu ◽  
Guo Chen ◽  
Tian Li ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Chemical Compounds ◽  
Bioactive Metabolites ◽  
Molecular Formula ◽  
Experimental Conditions ◽  
Chemical Structures ◽  
Pubchem Compound ◽  
Specific Distribution ◽  
Small Chemical ◽  
Metabolome Data

Abstract Mulberry is an important economic crop plant and traditional medicine. It contains a huge array of bioactive metabolites such as flavonoids, amino acids, alkaloids and vitamins. Consequently, mulberry has received increasing attention in recent years. MMHub (version 1.0) is the first open public repository of mass spectra of small chemical compounds (&lt;1000 Da) in mulberry leaves. The database contains 936 electrospray ionization tandem mass spectrometry (ESI-MS2) data and lists the specific distribution of compounds in 91 mulberry resources with two biological duplicates. ESI-MS2 data were obtained under non-standardized and independent experimental conditions. In total, 124 metabolites were identified or tentatively annotated and details of 90 metabolites with associated chemical structures have been deposited in the database. Supporting information such as PubChem compound information, molecular formula and metabolite classification are also provided in the MS2 spectral tag library. The MMHub provides important and comprehensive metabolome data for scientists working with mulberry. This information will be useful for the screening of quality resources and specific metabolites of mulberry. Database URL: https://biodb.swu.edu.cn/mmdb/

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