scholarly journals Metabolite profiling in Mango (Mangifera indica L.) pollen grains in relation to viability

2019 ◽  
Vol 14 (1) ◽  
pp. 33-42
Author(s):  
K S Shivashankara ◽  
G A Geetha ◽  
T K Roy

Mango productivity is affected mainly by irregular flowering, proportion of bisexual flowers, poor pollination and fertilization and fruit drop. Poor fruit set in some of the varieties may be associated with the lower pollen viability. The present experiment was initiated to assess the viability of pollen grains and their metabolites in three mango cultivars Amrapali, Alphonso and Totapuri which are differing in their fruit set intensity. The profiling of sugars, amino acids and some of the phytohormones were analysed using Liquid Chromatography-Mass Spectrometry (LC-MS/MS). Assessment of pollen grains in three mango cultivars indicated that free sugars such as fructose and glucose, and available amino acids including serine, proline, lysine, phenylalanine, alanine and glutamic acid were predominantly higher in all the cultivars. Phytohormones like IAA, IBA, ABA, GA,zeatin, jasmonic acid and salicylic acid were significantly different in low fruit setting cultivars Alphonso and Totapuri compared to high fruit setting cultivar Amrapali. In cv. Alphonso all the metabolites were higher at anthesis but later decreased drastically compared to cvs. Totapuri and Amrapali. Pollen viability percentage was significantly higher in cv. Amrapali than in cvs. Totapuri, Alphonso. Among all the cultivars, Amrapali maintained better chemical composition at anthesis and also at two hours after anthesis compared to cvs. Totapuri and Alphonso.

2015 ◽  
Vol 12 (2) ◽  
pp. 64-73
Author(s):  
M M Alam Patwary ◽  
M Mizanur Rahman ◽  
Shahabuddin Ahmad ◽  
M A Khaleque Miah ◽  
M H Rahman

An experiment was conducted at the Vegetable Research Field of Olericulture Division, Horticulture Research Centre, Bangladesh Agricultural Research Institute, Gazipur during October 2006 to March 2007 and May to September 2007 to evaluate the performance of tomato (Solanum lycopersicum) genotypes in summer and winter seasons. Early flowering was observed in summer compared to winter. Pollen viability decreased greatly during summer ranging from 30.44 % in TMS 003 to 86.08 % in C 11 compared to that in winter (70.33 % in C 61 to 100.00 % in VRT 002). Fruit set (%) markedly decreased in summer, which ranged from 4.69 % in TMS 017 to 39.15 % in C 51 while it ranged from and 49.00 % in TMS 008 to 90.01 % in HT 017 during winter. During summer, fruit set (%) exhibited positive significant and correlation with viable pollen grains (%). Yield per plant ranged from 1224 g in C 61 to 2670 g in VRT 003 and 37 g in TMS 015 to 94 g in C 11 in winter. The genotypes HT 019, C 11, C 21, C 41, C 51, HT 016 and HT 017 exhibited a considerable heat tolerance in relation to fruit setting ability. The Agriculturists 2014; 12(2) 64-73


2010 ◽  
Vol 40 (11) ◽  
pp. 2204-2214 ◽  
Author(s):  
E. R. Palupi ◽  
J. N. Owens ◽  
S. Sadjad ◽  
Sudarsono ◽  
D. D. Solihin

Teak ( Tectona grandis L.) is believed to have been introduced from India 400–500 years ago and there appear to be no old-growth natural forests. However, Indonesia has many teak plantation forests and 40%–50% of seeds for reforestation come from seed orchards and the remainder from plantations. In both, flower and fruit abortion results in low fruit set and thus seed production. We investigated flower and fruit development in a clonal seed orchard in East Java in 1999 and 2001 using cross- and open-pollinated trees. The rates of abortion using cross-pollinations showed a similar pattern but were lower than for open-pollinations. The highest rate of abortion in cross- and open-pollinations was during pollination and fertilization, ranging 11%–23% and 31%–32%/day, respectively, less during fruit initiation (5%–6% and 6%–8%/day) and the lowest was during fruit maturation (<0.5%/day). Cross-pollinations increased fruit production by 10-fold compared with open-pollinations. About 30% of flowers were pollinated with an average of five pollen grains per stigma in open-pollinations. Pollen viability varied among clones and time of day but was not affected by position of flowers in an inflorescence. We conclude that low pollination success and low pollen viability are major causes for low fruit production in teak and are related to insect pollinators.


2021 ◽  
Vol 19 (4) ◽  
pp. e0906-e0906
Author(s):  
Sedighehsadat Khaleghi ◽  

Aim of study: (i) to explore differences between eggplant flowers capable of setting fruit including long (LGs) and medium style flowers (MEs) and those which suffer from severe problems with fertility and fruit setting including short style ones (SRTs); (ii) to study the effect of plant growth regulators on floral morphology and fruit setting. Area of study: Isfahan University of Technology, Isfahan, Iran, 2017 and 2018. Material and methods: First the floral morphology and initial fruit setting of 13 eggplant genotypes from Iran were investigated. Then the differences between LGs and SRTs of two genotypes were explored. Finally, the effect of 1-naphthaleneacetic acid (NAA) and spermidine (Spd) on floral morphology and initial and final fruit setting of these two genotypes was determined. Main results: Results showed SRTs were not capable of fruit setting. Compared to SRTs, LGs had larger central canals, higher protein, total sugar, reducing sugar and K concentrations, as well as longer polar axis and pollen tubes and greater pollen viability. Although 1.5 mM Spd and 20 mg L-1 NAA resulted in increasing of LGs and MEs, and also total initial fruit set, surprisingly, no significant differences were observed in the final yield and final fruit set between the control and these treatments. Researching highlights: Since the rate of fruit dropping was higher in those treatments compared to the control, plants with more SRTs likely regulate their final load by abscising their flowers, and plants with more LGs regulate them by abscising their fruits.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 475B-475
Author(s):  
Mohamed A. Shaheen ◽  
Samir Z. El-Agamy

Floral biology of `Taifi' pomegranate was studied on trees grown in extreme high temperature and drought conditions. Measurement included flowering date, duration and type, pollen viability, diameter, intitial and horticultural fruit set percentage, and fruit drop percentage and fluctuation. Heat and drought stresses were found to greatly influence some floral characteristics in addition to fruit set and productivity of `Taifi' pomegranate.


2018 ◽  
Vol 10 (15) ◽  
pp. 13002-13005
Author(s):  
K. Subin ◽  
P. A. Jose ◽  
T. V. Sarath

Salacia fruticosa Wall. ex M.A. Lawson, an endemic species was studied for the reproductive biology as this species showed reduced fruit set and natural regeneration.  The stigma-anther proximity, an extremely low number of pollen grains, a short period of pollen viability, a sparse incidence of pollinators, protandrous and facultative autogamous nature of the flowers and a low percent in fruit set were identified as biological constraints for the species.  The incidence of seed pest was added to the poor seed and seedling bank and accelerated rarity process of the species.


2019 ◽  
Vol 47 (4) ◽  
pp. 1258-1263
Author(s):  
Deniz AKSOY ◽  
Zeynel DALKILIC

The objective of this study was to investigate flowering, pollen, and fruit set characteristics of locally registered pomegranate cultivars between two consecutive years. ‘Dr. Ercan 35’, ‘Efenar 35’, ‘Kamilbey 35’, and ‘Tezeren 35’ cultivars have been developed by cross-breeding programs. Open-, cross-, and self-pollination studies were performed with these cultivars using three trees in each replicate in the field. Number of anthers per flower, number of pollen grains per anther, viability, and germination tests were conducted in the laboratory. Fruit set ratio of self-pollinated combinations were lower than open-pollination. The fruit set ratio from self-pollination was obtained from ‘Dr. Ercan’ (60.3%), ‘Efenar 35’ (62.4%), ‘Kamilbey 35’ (50.5%), and ‘Tezeren 35’ (25.2%). The highest fruit set ratio (49.7%) was obtained from ‘Kamilbey 35’ × ‘Dr. Ercan 35’ combination. The highest number of characteristics was as follows: ‘Tezeren 35’ (402 anthers/hermaphrodite flowers), ‘Kamilbey 35’ (8550 pollen/anther, male flowers in the beginning of flowering), ‘Efenar 35’ (84.7% pollen viability, hermaphrodite flowers), ‘Kamilbey 35’ (71.2%, pollen germination, hermaphrodite flowers). In conclusion, at least one, two or more, pollinator cultivars are necessary for commercial plantations using with these recently released pomegranate cultivars.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********


2020 ◽  
Vol 40 (01) ◽  
Author(s):  
Arun Kumar

Apple accounts for more than 74 per cent of total fruit production in Himachal Pradesh. Poor fruit-set as well as heavy fruit drop due to improper pollination, moisture stress and adverse environmental conditions during flowering are the major barriers in dry temperate zone fruit production of apple. Apple tree blooms abundantly but only very few of them matures into fruit and it is the need of the hour to induce better fruiting with chemicals that ultimately augment the productivity. With such an intent the experiment was carried out. Nitrobenzene 20 % EW applied as a foliar treatment at three stages i.e. pink bud, petal fall and fruit setting stage. The highest number of fruits and flowers/plant was observed in T4 (3ml/liter) and T3 (2 ml/liter) of Nitrobenzene 20 % applied treatments. On the other hand the lowest number of flowers as well as fruits were recorded from T2 (1 ml/liter) and T1 (control). Among different treatments tested, 20% nitrobenzene applied at 3 ml/litre showed superior results in contrast to other nitrobenzene levels with enhancing flowering, fruit set, yield and quality.


2012 ◽  
Vol 61 (1) ◽  
pp. 107-113 ◽  
Author(s):  
Agnieszka Sękara ◽  
Monika Bieniasz

The aim of the investigations was to determine the regularity of pollination and fertilization as dependent on the phenotype of flower in eggplant. The experiment was conducted in 2005 and 2006 at the Agricultural University in Kraków, Poland. The object of the investigations was eggplant (<i>Solanum melongena</i> L.) 'Epic F<sub>1</sub>', grown in the open field. Flower phenotype (long-, medium- or short-styled), fruit setting and the number of seeds per fruit were recorded on experimental plants during the flowering period. The number of pollen tubes in the middle of the style and the number on fertilized ovules in ovaries were evaluated for all types of flowers. The course of pollination was different in particular types of eggplant flowers. Differences concerned only the number of pollen tubes and fertilized ovules of short, medium- and long-styled flowers and the number of seeds in the fruit. There was not observed any incompatibility in the growth on pollen tubes in the styles of all types of eggplant flowers. Stereomicroscopy observations of cross sections of the ovaries do not show differences in the formation and position of the ovules on the placenta. Short-styled flowers were characterized by significantly lower pollination and fruit set efficiency and they produced fruits with a significantly lower number of seeds. The low number of pollen tubes, fertilized ovules and seeds can be a straight consequence of the morphology of short-styled flowers: small-sized stigmas and spatial separation of anther pores and stigmas, which makes pollination diffi cult.


2011 ◽  
Vol 59 (3) ◽  
pp. 238 ◽  
Author(s):  
Mike Ramsey ◽  
Glenda Vaughton ◽  
Glendon D. Ascough ◽  
Steven D. Johnson

The balance between sexual and asexual reproduction can vary markedly in clonal plants. At one extreme, plants are sexually infertile and reproduction is solely clonal. Infertility can be caused by environmental and/or genetic factors, but the role of each is often unknown. Here we determine variation in sexual reproduction and explore the underlying factors causing sexual infertility in Cyrtanthus breviflorus Harv. We examined open- and cross-pollinated fruit set, ploidy using flow cytometry, pollen viability, pollinator visits to flowers and pollen deposition onto stigmas. One population was sexually infertile; no plants produced fruit. Three populations were sexually fertile; >98% of plants produced fruit. Percent pollen viability differed between infertile (18%) and fertile (97%) populations. The most likely cause of infertility was unequal ploidy. Plants in the infertile population were triploid, whereas those in fertile populations were diploid. Pollination factors were not related to infertility. In infertile and fertile populations, pollen-collecting insects visited flowers frequently, depositing 4-fold more pollen grains onto stigmas than the number of ovules per flower. Our study is the first to demonstrate infertility and triploidy in C. breviflorus. How triploidy became established despite high levels of pollinator activity remains a challenging question.


1970 ◽  
Vol 35 (3) ◽  
pp. 367-373 ◽  
Author(s):  
MS Alam ◽  
N Sultana ◽  
S Ahmad ◽  
MM Hossain ◽  
AKMA Islam

Eight hybrid tomato lines bred for heat tolerance by the Olericulture Division, BARI were studied to observe their fruit setting ability and yield performance under the hot, humid conditions at the Olericulture Farm of Bangladesh Agricultural Research Institute, Joydebpur, Gazipur during summer 2005. Percent fruit set in the lines was found to be within the range of 30 to 45 except C-7 (3×7) in which this was 52.85. The tallest plants having larger number of branches and the lowest flower drop were also observed in C-7 (3×7). Pollen viability ranged from 27.63 to 61.52 percent among the hybrids. The highest weight of individual fruits (56.02 g) and firmness (1.41 kg) was observed in C-5 (2×5). The largest fruit in respect of length and diameter was produced by C-8 (5x5). No significant variation was found among the lines in respect of days to 50% flowering and percent TSS. Significant difference wa observed for fruit number per plant ranging from 27 to 51. All of the lines produced remarkably high yields and C-7 (3x7) gave the highest yield per plant (1.73 kg) as well as per hectare (41.5 tons). The highest gross return (1867500 Tk/ha) and the maximum net return (1486748 Tk/ha) having the highest BCR (3.90) were recorded in C-7 (3×7). Keywords: Heat tolerant; tomato hybrid; hot; humid. DOI: 10.3329/bjar.v35i3.6442Bangladesh J. Agril. Res. 35(3) : 367-373


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