scholarly journals Verifikasi Alat Multimeter Digital untuk Deteksi Cepat Residu Insektisida dalam Sayuran

Agrikultura ◽  
2019 ◽  
Vol 29 (3) ◽  
pp. 124
Author(s):  
Asep Kurnia ◽  
Eman Sulaeman ◽  
Asep Nugraha Ardiwinata
Keyword(s):  
Gas Chromatography ◽  
Pesticide Residues ◽  
Rapid Detection ◽  
High Performance ◽  
Chemical Compounds ◽  
Electrical Current ◽  
Insecticide Residues ◽  
Pesticides Residue ◽  
Layer Chromatography ◽  
Detection And Quantification

ABSTRACTVerification of digital multimeter for rapid detection of insecticide residues on vegetablesThe instruments that usually used for detection of pesticide residues are Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC), and Thin Layer Chromatography (TLC), however, these instruments are expensive, take time, and need a large number of chemicals. Therefore, it is needed to develop rapid method in analyzing pesticide residues. One instrument that is potential for rapid detection of pesticide residues is digital multimeter. This method uses resistance differences of electrical current on chemical compounds. The aim of this research was to assess capability of digital multimeter for rapid detection of insecticide residues on vegetables by the aspects of precision, accuracy, linearity, and detection limit. The research was conducted in IAERI laboratory, Laladon, Bogor on January to December 2012. Insecticide residues that detected were organochlorine and organophosphate on some vegetables of potato, cabbage, carrot, onion, chili, and cucumber. The results showed that precision values of digital multimeter for detection organochlorine and organophosphate residues on vegetables were 0.02 to 2.46%. Linearity values of digital multimeter for detection endrin on onion and potato, heptachlor on onion, BHC on cucumber, chlorphyriphos on potato and cabbage, and dichlorvos on cabbage were 0.81, 0.84, 0.95, 0.71, 0.95, 0.80 and 0.76, respectively. Accuracy values of digital multimeter for detecting endrin and dieldrin on onion, chlorphyriphos on potato and cabbage, and profenophos on chili were 106.93%, 115.08%, 72.89%, 72.40%, and 98.06%, respectively. Detection and quantification limits of digital multimeter for detecting organochlorine were 0.08 to 0.17 ppm and 0.27 to 0.56 ppm, respectively. Meanwhile, detection and quantification limits of digital multimeter for detecting organophosphate were 0.05 to 0.27 ppm and 0.16 to 0.90 ppm, respectively. This result showed that digital multimeter was accurate and representative instrument for detecting pesticide residues on vegetables.Keywords: Digital Multimeter, Pesticides residue, VegetablesABSTRAKAlat yang digunakan untuk analisis residu pestisida antara lain Kromatografi Gas (KG), Kromatografi Cair Kinerja Tinggi (KCKT), Kromatografi Lapis Tipis (KLT), namun alat tersebut sangat mahal, dan perlu proses cukup lama serta memerlukan bahan kimia yang banyak. Untuk itu perlu dikembangkan metode cepat analisis residu pestisida. Salah satu alternatif deteksi cepat residu pestisida adalah dengan menggunakan Alat Multimeter Digital (AMD). Teknik analisis ini menggunakan prinsip perbedaan resistensi (tahanan) terhadap arus listrik dari senyawa kimia residu pestisida. Percobaan ini bertujuan mengetahui cara kerja awal AMD untuk deteksi cepat residu insektisida pada tanaman sayuran dari aspek presisi, akurasi, linearitas, dan limit deteksi. Penelitian dilaksanakan di Laboratorium Balai Penelitian Lingkungan Pertanian (Balingtan) di Laladon Bogor, mulai bulan Januari hingga Desember 2012. Residu insektisida yang dideteksi adalah organoklorin dan organofosfat pada beberapa sayuran yaitu kentang, kubis, wortel, bawang merah, cabe merah dan mentimun. Hasil penelitian menunjukkan bahwa nilai presisi AMD dalam mendeteksi organoklorin dan organofosfat pada sayuran berkisar 0,02-2,46%. Nilai linearitas AMD dalam mendeteksi endrin pada bawang merah dan kentang, heptaklor pada bawang merah, BHC pada mentimun, klorpirifos pada kentang dan kubis, serta diklorfos pada kubis masing-masing sebesar 0,81, 0,84, 0,95, 0,71, 0,95, 0,80 dan 0,76. Nilai akurasi AMD dalam mendeteksi endrin dan dieldrin pada bawang merah, klorpirifos padakentang dan kubis, serta profenofos pada cabe merah masing-masing sebesar 106,93 %, 115,08%, 72,89 %, 72,40%, dan 98,06%. Nilai LOD (limit deteksi) dan LOQ (limit quantifikasi) AMD terhadap organoklorin berkisar antara 0,08-0,17 ppm dan 0,27-0,56 ppm, sedangkan terhadap golongan organofosfat berkisar antara 0,05-0,27 ppm dan 0,16-0,90 ppm. Hasil penelitian ini menunjukkan bahwa AMD merupakan alat yang cukup akurat dan representatif untuk deteksi cepat residu pestisida pada sayuran.Kata Kunci: Alat Multimeter Digital, Residu pestisida, Sayuran

scholarly journals Verifikasi Alat Multimeter Digital untuk Deteksi Cepat Residu Insektisida dalam Sayuran

Agrikultura ◽  
2018 ◽  
Vol 29 (3) ◽  
pp. 124
Author(s):  
Asep Kurnia ◽  
Eman Sulaeman ◽  
Asep Nugraha Ardiwinata
Keyword(s):  
Gas Chromatography ◽  
Pesticide Residues ◽  
Rapid Detection ◽  
High Performance ◽  
Chemical Compounds ◽  
Electrical Current ◽  
Insecticide Residues ◽  
Pesticides Residue ◽  
Layer Chromatography ◽  
Detection And Quantification

ABSTRACTVerification of digital multimeter for rapid detection of insecticide residues on vegetablesThe instruments that usually used for detection of pesticide residues are Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC), and Thin Layer Chromatography (TLC), however, these instruments are expensive, take time, and need a large number of chemicals. Therefore, it is needed to develop rapid method in analyzing pesticide residues. One instrument that is potential for rapid detection of pesticide residues is digital multimeter. This method uses resistance differences of electrical current on chemical compounds. The aim of this research was to assess capability of digital multimeter for rapid detection of insecticide residues on vegetables by the aspects of precision, accuracy, linearity, and detection limit. The research was conducted in IAERI laboratory, Laladon, Bogor on January to December 2012. Insecticide residues that detected were organochlorine and organophosphate on some vegetables of potato, cabbage, carrot, onion, chili, and cucumber. The results showed that precision values of digital multimeter for detection organochlorine and organophosphate residues on vegetables were 0.02 to 2.46%. Linearity values of digital multimeter for detection endrin on onion and potato, heptachlor on onion, BHC on cucumber, chlorphyriphos on potato and cabbage, and dichlorvos on cabbage were 0.81, 0.84, 0.95, 0.71, 0.95, 0.80 and 0.76, respectively. Accuracy values of digital multimeter for detecting endrin and dieldrin on onion, chlorphyriphos on potato and cabbage, and profenophos on chili were 106.93%, 115.08%, 72.89%, 72.40%, and 98.06%, respectively. Detection and quantification limits of digital multimeter for detecting organochlorine were 0.08 to 0.17 ppm and 0.27 to 0.56 ppm, respectively. Meanwhile, detection and quantification limits of digital multimeter for detecting organophosphate were 0.05 to 0.27 ppm and 0.16 to 0.90 ppm, respectively. This result showed that digital multimeter was accurate and representative instrument for detecting pesticide residues on vegetables.Keywords: Digital Multimeter, Pesticides residue, VegetablesABSTRAKAlat yang digunakan untuk analisis residu pestisida antara lain Kromatografi Gas (KG), Kromatografi Cair Kinerja Tinggi (KCKT), Kromatografi Lapis Tipis (KLT), namun alat tersebut sangat mahal, dan perlu proses cukup lama serta memerlukan bahan kimia yang banyak. Untuk itu perlu dikembangkan metode cepat analisis residu pestisida. Salah satu alternatif deteksi cepat residu pestisida adalah dengan menggunakan Alat Multimeter Digital (AMD). Teknik analisis ini menggunakan prinsip perbedaan resistensi (tahanan) terhadap arus listrik dari senyawa kimia residu pestisida. Percobaan ini bertujuan mengetahui cara kerja awal AMD untuk deteksi cepat residu insektisida pada tanaman sayuran dari aspek presisi, akurasi, linearitas, dan limit deteksi. Penelitian dilaksanakan di Laboratorium Balai Penelitian Lingkungan Pertanian (Balingtan) di Laladon Bogor, mulai bulan Januari hingga Desember 2012. Residu insektisida yang dideteksi adalah organoklorin dan organofosfat pada beberapa sayuran yaitu kentang, kubis, wortel, bawang merah, cabe merah dan mentimun. Hasil penelitian menunjukkan bahwa nilai presisi AMD dalam mendeteksi organoklorin dan organofosfat pada sayuran berkisar 0,02-2,46%. Nilai linearitas AMD dalam mendeteksi endrin pada bawang merah dan kentang, heptaklor pada bawang merah, BHC pada mentimun, klorpirifos pada kentang dan kubis, serta diklorfos pada kubis masing-masing sebesar 0,81, 0,84, 0,95, 0,71, 0,95, 0,80 dan 0,76. Nilai akurasi AMD dalam mendeteksi endrin dan dieldrin pada bawang merah, klorpirifos pada kentang dan kubis, serta profenofos pada cabe merah masing-masing sebesar 106,93 %, 115,08%, 72,89 %, 72,40%, dan 98,06%. Nilai LOD (limit deteksi) dan LOQ (limit quantifikasi) AMD terhadap organoklorin berkisar antara 0,08-0,17 ppm dan 0,27-0,56 ppm, sedangkan terhadap golongan organofosfat berkisar antara 0,05-0,27 ppm dan 0,16-0,90 ppm. Hasil penelitian ini menunjukkan bahwa AMD merupakan alat yang cukup akurat dan representatif untuk deteksi cepat residu pestisida pada sayuran.Kata Kunci: Alat Multimeter Digital, Residu pestisida, Sayuran

Quantitative Determination of Vitamin E Isomers using Gas Chromatography, High Performance Liquid Chromatography and High Performance Thin Layer Chromatography

2017 ◽  
Vol 54 (3) ◽  
pp. 294
Author(s):  
Hari Ramakrishnan K. ◽  
Janaky Ranjithkumar
Keyword(s):  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin E ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Highly Sensitive ◽  
Layer Chromatography

Vitamin E, the fat soluble vitamin is present naturally in some foods and added in food supplements, nutraceuticals etc due to its vital biological function as an antioxidant. Various methods are available for the analysis of vitamin E. Especially High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are exclusively used for the quantitative evaluation of vitamin E, which has also identified the four different isomeric forms of this vitamin. The rate of losses of this vitamin during food processing and analysis, in addition to their transient dynamics, presents complexities in developing a highly sensitive procedure for their separations. Though effective, HPLC instrument is expensive and comparatively cumbersome. In this prospective, the study was to evaluate the usefulness of High Performance Thin Layer Chromatography (HPTLC) in the analysis of vitamin E. There are methods available using Thin Layer Chromatography for its analysis, but they are not sensitive enough to identify the isomeric forms of vitamin E. In this HPTLC method, the different isomeric forms of vitamin E - α, β, γ and δ were identified. This technique shall be considered as an alternative to the other methods such as HPLC and GC.

scholarly journals High-Performance Thin-Layer Chromatographic Analysis of Selected Organophosphorous Pesticide Residues in Tea

2008 ◽  
Vol 91 (5) ◽  
pp. 1210-1217 ◽  
Author(s):  
Yongde Yue ◽  
Rong Zhang ◽  
Wei Fan ◽  
Feng Tang
Keyword(s):  
Thin Layer ◽  
Pesticide Residues ◽  
Chromatographic Analysis ◽  
High Performance ◽  
Solid Phase ◽  
Relative Standard ◽  
Precision And Accuracy ◽  
Thin Layer Chromatographic Analysis ◽  
Layer Chromatography ◽  
Hptlc Method

Abstract The separation of 9 organophosphates (monocrotophos, quinalphos, triazophos, parathion-methyl, isofenphos-methyl, temephos, parathion, phoxim-ethyl, and chlorpyrifos) by high-performance thin-layer chromatography (HPTLC) with automated multiple development was studied. The HPTLC method was developed and validated for analysis of residues of phoxim-ethyl and chlorpyrifos in tea. The sample was extracted with acetonitrile and cleaned up by ENVI-CARB solid-phase extraction. The extract was directly applied as bands to glass-backed silica gel 60F254 HPTLC plates. The plates were developed with dichloromethanehexane (1 1, v/v) in a glass twin-trough chamber. Evaluation of the developed HPTLC plates was performed densitometrically. The results indicated that the detection limits of phoxim and chlorpyrifos were 5.0 109 and 1.0 108 g, respectively. Recoveries of the pesticides from tea by this analytical method were 90.7105.5%, and relative standard deviations were 7.313.5%. The precision and accuracy of the method were generally satisfactory for analysis of pesticide residues in tea.

scholarly journals Phytochemical characterization of the ethanolic extract of Kaempferia galanga rhizome for anti-oxidant activities by HPTLC and GCMS

2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Flavius Phrangsngi Nonglang ◽  
Abhijeet Khale ◽  
Surya Bhan
Keyword(s):  
Gas Chromatography ◽  
High Performance ◽  
Freeze Drying ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Kaempferia Galanga ◽  
Anti Oxidant ◽  
Gas Chromatography Mass Spectroscopy ◽  
Layer Chromatography

Abstract Background The rhizome of Kaempferia galanga (K. galanga) was collected from Meghalaya, India, and its ethanolic extract was obtained by freeze-drying or lyophilization process, which was then assessed for its in vitro anti-oxidant activity and phytochemical characterization using high-performance thin-layer chromatography (HPTLC) and gas chromatography-mass spectroscopy (GCMS). Results In vitro anti-oxidant activity analysis shows an inhibitory concentration (IC50) value of 1.824 mg/mL and 0.307 mg/mL for, α, α-diphenyl-ρ-picrylhydrazyl (DPPH) and 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays, respectively. Total polyphenol content (TPC) of 23.55 ± 0.5 mg gallic acid equivalent (GAE)/g dry weight of extract and total flavonoid content (TFC) of 100 ± 1.414 mg rutin equivalents (RE)/g dry weight of extract were found. High-performance thin-layer chromatography (HPTLC) analysis shows the best separation of bands at different retention factor (Rf) values, when employing the solvent system 2-butanol/1-propanol/water in the ratio of 3:1:1 (v/v/v). Gas chromatography-mass spectroscopy (GCMS) analysis confirms the presence and identification of various phytocompounds, with ethyl p-methoxycinnamate identified as the major active compound. Conclusion Freeze-dried ethanolic extract of K. galanga (rhizome) possesses anti-oxidant activity. Ethyl p-methoxycinnamate is present as the major bioactive component (about 94.87% of the total area composition), and since it has very important and diverse medicinal properties, a freeze-drying process (lyophilization) can be utilized for its isolation and extraction.

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