PHYTOCHEMICAL TEST OF MANGROVE Avicennia alba, Rhizopora apiculata AND Sonneratia alba FROM MUSI RIVER ESTUARY, SOUTH SUMATERA

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Nadya Rahmania ◽  
Herpandi Herpandi ◽  
Rozirwan Rozirwan

Mangrove is one of the plants that has the potential to be developed into medicinal plants. However, further research is needed to prove scientifically the content of secondary metabolites in it. This study aims to identify the secondary metabolites contained from leaves and roots in Avicennia alba, Rhizopora apiculata and Sonneratia alba. The leaves and roots of mangroves extracted by maceration using ethyl acetate. The secondary metabolites contained in mangrove samples obtained by doing phytochemical tests through color tests. Phytochemical test results showed that secondary metabolites contained in mangrove Avicennia alba are flavonoids, steroids/triterpenoids, saponins, and tannins/phenols in leaf samples, whereas in its root samples it contains flavonoids and steroids/triterponoid compounds. Sonneratia alba contains flavonoids, steroids/teriterpenoids, saponins and tannins/phenols in its leaf samples, while the root samples contain flavonoids, steroids/teriterpenoids and tannins/phenols compounds. The leaf samples of mangrove Rhizopora apiculatacontains flavonoid and steroid/triterpenoid compounds whereas in its root samples contains flavonoids, steroids/triterpenoids, saponins as well as tannins/phenols.

2021 ◽  
Vol 26 (2) ◽  
pp. 228-234
Author(s):  
Harum Farahisah ◽  
Fredinan Yulianda ◽  
Hefni Effendi

Mangrove ecosystem services, aside for the fisheries sector, also have ecosystem services in mitigating climate change as carbon sinks and storage. Musi river estuary located in Banyuasin Regency, South Sumatra has the potential of mangrove forests as carbon reserves. The purpose of the study is to analyze the community structure, potential carbon stocks, and economic value of mangroves in the Musi River Estuary. Data were collected at five stations using the 100 m quadratic transect method vertically to the coastline. Data analysis was conducted based on the allometric equation for biomass information, carbon storage, and the economic value of carbon stores. Mangrove ecosystems in the Musi River estuary are Sonneratia alba, Nypa fruticans, Sonneratia casiolaris, and Rhizopora apiculata. Mangrove species that have important roles in the sustainability of ecosystems are Nypa fruticans and Sonneratia alba each with IVI of 145.6 and 101.28, respectively. Carbon storage of mangrove in the Musi river estuary is 107.34 tonC / ha and carbon dioxide absorption is 393.59 tCO2 / ha with a mangrove area of ± 5276 ha. The total estimated economic value of carbon generated from mangrove are Rp174.441.016.895 (free market) and Rp455.872.524.151 (CDM). Keywords: carbon, mangrove, Musi river estuary


Author(s):  
Verly Dotulong ◽  
Djuhria Wonggo ◽  
Lita A. D. Y. Montolalu

The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate  fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol  (3.43±0.25 µg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.


RSC Advances ◽  
2020 ◽  
Vol 10 (17) ◽  
pp. 10221-10231
Author(s):  
Tahira Younis ◽  
Faiza Jabeen ◽  
Laila Jafri ◽  
Azhar Rasul ◽  
Maleeha Manzoor ◽  
...  

Secondary metabolites present in medicinal plants offer a golden opportunity to fight different ailments, such as cancer, infections, diabetes, neurodegenerative and cardiovascular diseases, etc.


2019 ◽  
Vol 18 (1) ◽  
pp. 13-20
Author(s):  
Mohammad Sayuti ◽  
Widya Dwi Rukmi Putri ◽  
Yunianta

Secondary metabolites of the axis section (modulla skeleton) of sea bamboo (Isis hippuris) were identified by GC-MS. The dominant compounds using GC-MS in the ethanol fraction were hexanedioic acid (11.85%), acetamide (9.46%), n-hexadecanoic acid (9.22%) and thiosulfuric acid (7.22%). On the other side, the dominant compounds of the test results in the ethyl acetate fraction were 7-oxabicyclo[4.1.0] heptanes (28.27%), 1,2- benzene dicarboxylic acid (14.77%), cis-8-(N-pyrrolidyl)-(2,2,5,5-tetradeutero)bicyclo[4.3.0]nona-3,7-diene (9.98%), hexahydropyridine (7,86%). The dominant compounds of the n-hexane fraction were hexanedioic acid (41.99%), azetidine with a peak number of 40 (9.98%), 1-octadecene (8,36%). Antioxidant activities were also evaluated by DPPH scavenging assay. The ethanol, n-hexane and ethyl acetate fractions showed total flavonoid contents of 7.86% ± 0.12, 12.97% ± 0.36, 1.88% ± 0.26, respectively and the IC50 in the antioxidant assay were 480.25±74,74, 469.50±19,13, 3221.07±138,69 respectively. Dhaka Univ. J. Pharm. Sci. 18(1): 13-20, 2019 (June)


2021 ◽  
Vol 12 (6) ◽  
pp. 7584-7595

Medicinal plants are a promising host for endophytic fungi to produce secondary metabolites relevant for food and health. In this study, we evaluate antioxidant activity to determine the species of endophytic fungi isolated from Syzygium aqueum. Endophytic fungi were isolated from leaf stalks through surface sterilization. The fungi’s isolate was identified with morphology and molecular analysis (ITS-rDNA). The pure fungi strain was cultivated on PDB media for 4 weeks, and metabolites were extracted using ethyl acetate. The crude extract of endophytic fungi was examined for its antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The pure compound was isolated using the chromatography method, and its structure was determined using spectroscopy analysis involving NMR 1D and 2D. In total, four obtained endophytic fungi were isolated from leaf stalks. The fungi with good antioxidant activity (IC50 59.2 µg/mL) were identified as Beltrania rhombica. The characteristics of the pure compound are white-yellowish powder with IC50 44.2 µg/mL. Based on spectroscopy analysis, the pure compound was identified as 3-(hydroxyl(3,4,5-trihydroxylphenyl)methyl)-3,4-dihydro-2H-pyran-4,5,6-triol.


2017 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Mamay Maslahat ◽  
Febi Nurilmala ◽  
Lipi Harpeni

Antioxidant Activity of Aquous Extract of Sembung Leaf (Blumea balsamifera)             Simplicia medicinal plants are more commonly known by the term of herbal medicine in the form boiling and steeping. Sembung leaf water extracts content of secondary metabolites has potential natural antioxidant. An antioxidant is a substance that can prevent or slow down the deterioration of a substance. This research aimed to test the potential of antioxidant in water extracts of leaves sembung (Blumea balsamifera) in the form of boiling and steeping using DPPH method through determination of IC50 values. Best antioxidant test results found in extracts of steeping the leaves with IC50 values of 155,65 ml/l while the extract boiling sembung with IC50 values indicated by 293,80 ml/l, antioxidant standards testing used was quercetin.Keywords : antioxidant, aquous  extract, Blumea balsamifera, DPPH ABSTRAK         Simplisia tanaman obat yang lebih dikenal dengan istilah jamu sering diaplikasikan dalam bentuk godogan dan seduhan. Ekstrak air daun sembung memiliki kandungan metabolit sekunder yang berpotensi sebagai antioksidan alami. Antioksidan merupakan substansi yang dapat mencegah atau memperlambat kerusakan suatu zat. Penelitian ini bertujuan menguji potensi antioksidan ekstrak air daun sembung (Blumea balsamifera) dalam bentuk seduhan dan godogan dengan metode DPPH melalui penentuan nilai IC50.  Hasil uji antioksidan terbaik terdapat pada ekstrak seduhan daun sembung dengan nilai IC50 sebesar 155,65 ml/l sedangkan ekstrak godogan sembung menunjukan nilai IC50 sebesar 293,80 ml/l,  sebagai standar pengujian antioksidan digunakan kuersetin.Kata kunci : Antioxidan, extract air, Blumea balsamifera, DPPH


Author(s):  
Imane Chamkhi ◽  
Taoufiq Benali ◽  
Tarik Aanniz ◽  
Nawal Elmenyiy ◽  
Fatima-Ezzahrae Guaouguaou ◽  
...  

Author(s):  
A Rajasekaran ◽  
V Abirami ◽  
S Arunkumar ◽  
Priya G Bharani ◽  
S Gugapriya ◽  
...  

Cardiospermum halicacabum is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases, mainly for arthritis. Apigenin is one of the major constituent present in Cardiospermum halicacabum. The present study mainly aimed to estimate the content of major constituent apigenin present in Cardiospermum halicacabum collected from 21 districts of Tamil Nadu by HPTLC method using the marker compound apigenin. The HPTLC method was performed using HPTLC aluminium sheets precoated with Silica Gel 60 GF254 as stationary phase and Toluene: Ethyl acetate: formic acid: methanol (3:6:1.6:0.4 v/v) as the mobile phase. The developed chromatogram was scanned at 254nm using Camag Scanner III. The Rf value of standard apigenin and apigenin in the leaf extract of Cardiospermum halicacabum was found to be in the range of 0.80 to 0.89. Plant collected from Cuddalore district of Tamil Nadu was found to contain relatively high amount of marker compound apigenin than other regions.


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