The effect of embryo donors and recipients of on milk efficiency of their female offsprings from embryo-transfer was followed. Heifers and cows of Czech Spotted Breed were used as a embryo donors and recipients. The selected characteristics of milk efficiency – the quantity of milk (kg), the quantity of proteins (kg) and content of proteins (%) were analysed by using of variation-statistical methods and analysis of variance (programme Statistica, version 6.0) by the donors (31), recipients (74) and their daughters (74) from embryotransfer. These selected characterictics were analysed in the 1st and in the maximal lactations. Milk efficiency of the recipients was the lowest in the 1st and maximal lactation; the recipients produced 4991.4 kg, and/or 6082.86 kg of milk and 174.79 kg, and/or 213.5 kg of protein; however, this differences were significant (P < 0.05 and P < 0.01). The protein content in % in milk of recipients was 3.45% in the 1st and maximal lactation; i. e. 0.05% more protein (insignificant difference), and/or 0.13% more protein than the donors (significant difference, P < 0.05). Milk efficiency was the highest in the offspring in the 1st and maximal lactation; the daughters from ET produced 938.5 kg, and/or 1804.7 kg of milk more (P < 0.01), 41.28 kg, and/or 67.32 kg of protein more (P < 0.01), and 0.14% (P < 0.01), and/or 0.11% (P < 0.01) of protein more than the recipients, and 305.48 kg, and/or 823.26 kg of milk more (P > 0.05), 0.19%, and/or 0.24% of protein more (P < 0.01) and 28.55 kg of protein more (P > 0.05) in the maximal lactation than the donors. In the 1st lactation the daughters from ET produced 2.58 kg less protein than the donors; however, this difference was insignificant (P > 0.05). There was found the effect of donors was significant on milk efficiency of their daughters and the effect of recipients was no significant on milk efficiency of their female offsprings from embryotransfer. There was found too that already the milk efficiency from first lactations could be using for selection of embryo donors.