scholarly journals Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

2020 ◽  
Vol 7 (1) ◽  
pp. 59-70
Author(s):  
Ines Sekhara ◽  
Ouahiba Benaissa ◽  
Amel Amrani ◽  
Beretta Giangiacomo ◽  
Wassila Benabderrahmane ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Study ◽  
Aerial Parts ◽  
Lipid Peroxidation Inhibition ◽  
Ethyl Acetate Extracts ◽  
In Vitro Antioxidant Activity

AbstractThe chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10.95%). The phytochemical study led to the isolation and structural elucidation of three compounds, scopoletin, umckalin and 1-(3’,4’-dihydroxycinnamoyl) cyclopentane-2,3-diol. Moreover the ethyl acetate extract was screened for its possible in vitro antioxidant activity by 2,2-diphenyl-1-picrylhydrazy l(DPPH) and lipid peroxidation inhibition assays in which it displayed a noticeable activity. This study provides the first biological and chemical investigation on Anthriscus vulgaris Bernh. in Algeria.

scholarly journals In vitro antioxidant activity of crude extracts of Harpagophytum zeyheri and their anti-inflammatory and cytotoxicity activity compared with diclofenac

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sibonokuhle F. Ncube ◽  
Lyndy J. McGaw ◽  
Emmanuel Mfotie Njoya ◽  
Hilton G. T. Ndagurwa ◽  
Peter J. Mundy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inflammatory Activity ◽  
In Vitro Assays ◽  
Tnf Α ◽  
Low Toxicity ◽  
Anti Inflammatory ◽  
Ethyl Acetate Extracts ◽  
In Vitro Antioxidant Activity

Abstract Background This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac. Methods In vitro assays were conducted using water, ethanol, and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2′-diphenyl-1-picrylhydrazy (DPPH) and 2,2′- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results The ethyl acetate extract had the lowest IC50 values in the DPPH (5.91 μg/ml) and ABTS (20.5 μg/ml) assay compared to other extracts. Furthermore, the ethyl acetate extracts effectively inhibited NO and TNF-α and proved to be comparable to diclofenac at some concentrations. All extracts of H. zeyheri displayed dose-dependent activity and were associated with low levels of human-IL-10 expression compared to quercetin. Furthermore, all extracts displayed low toxicity relative to diclofenac. Conclusions These findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in the inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.

scholarly journals ISOLASI DAN IDENTIFIKASI SENYAWA ANTIOKSIDAN GOLONGAN FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN PRANAJIWA (Euchresta horsfieldii Lesch Benn.)

2017 ◽  
Vol 5 (1) ◽  
pp. 26
Author(s):  
Ni Wayan Rika Kumara Dewi ◽  
I Wayan Gunawan ◽  
Ni Made Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Infrared Spectra ◽  
Ethyl Acetate Extract ◽  
Total Flavonoid ◽  
Separation And Purification ◽  
Ic50 Value ◽  
Ethyl Acetate Extracts ◽  
In Vitro Antioxidant Activity

ABSTRAK: Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan mengidentifikasi senyawa golongan flavonoid yang terkandung dalam ekstrak etil asetat daun pranajiwa (Euchresta horsfieldii Lesch Benn.). Uji aktivitas antioksidan dilakukan secara invitro dengan metode DPPH (1,1-diphenyl-2-pycrylhidrazyl). Teknik pemisahan dilakukan dengan kromatografi kolom dan diidentifikasi dengan spektrofotometer FTIR dan UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan dengan nilai IC50 sebesar 393,95 µg/mL dan kadar total flavonoid sebesar 6619,72 mg QE/100g atau 6,62 % QE. Pemisahan dan pemurnian ekstrak etil asetat menggunakan fase diam silika gel 60 dan fase gerak n-heksana:kloroform: etanol (20:1:1) yang menghasilkan isolat aktif yang positif flavonoid (isolat FE) yang diduga merupakan senyawa flavonoid golongan flavonol yaitu 3,5,7,3’,4’-pentahidroksi flavonol.   ABSTRACT: The present study was conducted to determine antioxidant activity and to identify the flavonoid active compounds from ethyl acetate extracts of Pranajiwa leaves. In vitro antioxidant activity was carried out using DPPH (1,1-diphenyl-2-pycrylhidrazyl) method. The separation of the ethyl acetate extracts was conducted by column chromatography and the identification by using FTIR and UV-Vis spectrophotometer. The results showed that ethyl acetate extract has antioxidant activity with IC50 value of 393,95 µg/mL and the total flavonoid of 6619,72 mg QE /100g or 6,62% QE. The separation and purification of  ethyl acetate extract was chromatography column on silica gel 60 and the solvent n-hexane:chloroform:ethanol (20:1:1) based on UV-Vis and Infrared spectra positive flavonoid isolate (isolates FE) was tentaviely identified as 3,5,7,3',4'-pentadihydroxy flavonols.    

Chemical Constituents of the Aerial Parts of Scoparia Dulcis and Anti-Cancer, Anti-Inflammatory Activities

2019 ◽  
Vol 814 ◽  
pp. 360-364
Author(s):  
Nguyen Ngoc Thanh Tin ◽  
Nguyen Dang Thanh Truc ◽  
Hoang Thi Thu Hang ◽  
Pham Thi Nhat Trinh ◽  
Long Giang Bach ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Chemical Constituents ◽  
Methanol Fraction ◽  
Scoparia Dulcis ◽  
Aerial Parts ◽  
Anti Cancer ◽  
Anti Inflammatory ◽  
Whole Plants ◽  
Ethyl Acetate Extracts

In Vietnam, the medicinal plant Scoparia dulcis is used for the treatment of bronchitis, gastric disorders, antidiabetes, hypertension, hepatitis etc.1 In this paper, experiments was designed to evaluate in vitro anticancer and anti-inflammatory activities and isolate phytochemicals from methanol extracts of Scoparia dulcis whole plants. The chemical investigation of methanol fraction of Scoparia dulcis led to the isolation of benzoxazinone (1), phenylethanoid (2), flavone (3), and lignan (4) glycosides. The bioactivity results indicated that crude ethanol and ethyl acetate extracts had potent cytotoxic activity toward the HepG2 cancer cell with IC50 of 47.03 μg/mL and 36.04 μg/mL, respectively. Interestingly, crude ethanol and ethyl acetate extracts had the NO inhibitory activity, with inhibition of 56.9 % or 74.7 % at 30 μg/mL and 97.3% or 82.9 % at 100 μg/mL, respectively. In addition, the hexane extract at concentrations of 100 μg/ml demonstrated anti-inflammatory activity through the inhibition of nitric oxide production of 76.7%.

In Vitro Antioxidant Activity Metaplexis Japonica Makino Extract and Derivatives

2014 ◽  
Vol 955-959 ◽  
pp. 387-389 ◽  
Author(s):  
Bao Qing Wang
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Test ◽  
Acetone Extracts ◽  
Β Carotene ◽  
Ethyl Acetate Extracts

Antioxidant activities of acetone and ethyl acetate extracts from Metaplexis japonica Makino, one of famous medicine plants in the eastnorth region of China, named luomo in Chinese, were examined by a DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical-scavenging assay and a β-carotene-linoleic acid test. In DPPH, the antioxidant activity of the acetone extracts, ethyl acetate extracts and derivative were IC50 were 313.21, 266.92 and 118.78μg/mL, respectively. In the β-carotene-linoleic acid test, IC50 were 285.09, 351.57 and 123.89μg/mL. It was concluded that Metaplexis japonica Makino and its derivatives might be a potential natural source of antioxidants .

scholarly journals In Vitro Antioxidant Activity of Crude Extracts of Harpagophytum Zeyheri and Their Anti-Inflammatory and Cytotoxicity Activity Compared With Diclofenac.

2021 ◽  
Author(s):  
Sibonokuhle F. Ncube ◽  
Lyndy J. McGaw ◽  
Emmanuel Mfotie Njoya ◽  
Hilton G.T. Ndagurwa ◽  
Peter J. Mundy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Inflammatory Activity ◽  
In Vitro Assays ◽  
Cytotoxicity Activity ◽  
Tnf Α ◽  
Low Toxicity ◽  
Anti Inflammatory ◽  
Ethyl Acetate Extracts

Abstract Background This study evaluated the in vitro antioxidant activity and comparison of anti-inflammatory and cytotoxic activity of Harpagopytum zeyheri with diclofenac. Methods In vitro assays were conducted using water, ethanol and ethyl acetate extracts of H.zeyheri. The antioxidant activity was evaluated using the 2,2'-diphenyl-1-picrylhydrazy (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)assays. The anti-inflammatory activity was determined by measuring the inhibition of nitric oxide (NO) on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages as well as cytokine (TNF-α and IL-10) expression on LPS-induced U937 human macrophages. For cytotoxicity, cell viability was determined using the 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results The ethyl acetate extract had the lowest IC50 values in the DPPH (5.91µg/ml) and ABTS (20.5µg/ml) assay compared to other extracts. Furthermore, the ethyl acetate extracts effectively inhibited NO and TNF-α and proved to be comparable to diclofenac at some concentrations. All extracts of H. zeyheri displayed dose dependent activity and were associated with low levels of human-IL-10 expression compared to quercetin. Furthermore, all extracts displayed low toxicity relative to diclofenac. Conclusions These findings show that H. zeyheri has significant antioxidant activity. Additionally, similarities exist in inflammatory activity of H. zeyheri to diclofenac at some concentrations as well as low toxicity in comparison to diclofenac.

scholarly journals in vitro Antioxidant Activity of Ethyl Acetate Fraction of Fermentation Products of Ginkgo biloba

2014 ◽  
Vol 26 (21) ◽  
pp. 7351-7354 ◽  
Author(s):  
Xuan Liu ◽  
Xin Zhan ◽  
Xianwei Zou ◽  
Yun Huang ◽  
Qian Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ginkgo Biloba ◽  
Ethyl Acetate Fraction ◽  
Fermentation Products ◽  
In Vitro Antioxidant Activity

scholarly journals Assessment of Antioxidant effect of the essential oil and methanol extract of Centaurea dimorpha Viv. aerial parts from Algeria.

2019 ◽  
Vol 6 (1) ◽  
pp. 54-62
Author(s):  
Abdelwahab Belkassam ◽  
Amar Zellagui ◽  
Noureddine Gherraf ◽  
Guido Flamini ◽  
Pier Luigi Cioni ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Tetradecanoic Acid ◽  
Aerial Parts ◽  
In Vitro Antioxidant Activity

Abstract The essential oils obtained by hydrodistillation of aerial parts of Centaurea dimorpha Viv. were analyzed by means of gas chromatography-mass spectrometry (GC-MS). The main constituents were Caryophyllene oxide (09.88%), limonene (5.73%), tetradecanoic acid (5.68%), spathulenol (5.44%), methyl hexadecanoate (4.45%), and α-pinene (03.08%). To our best knowledge this is the first study of essential oils from the aerial parts of Centaurea dimorpha growing in Algeria. Moreover the essential oil and methanolic extract were screened for their possible in vitro antioxidant activity by DPPH free radical-scavenging test. The findings showed that the percentage inhibition is 10.67 and 77.01 respectively at a concentration of 1mole/L.

scholarly journals Biochemical Profile, In vitro Toxicity, and Cytotoxic activity of Eragrostis amabilis (L.) Wight. Arn. and Eragrostis pilosa (L.) Beauve

2021 ◽  
Vol 11 (2) ◽  
pp. 3480-3487
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Total Phenolics ◽  
Maximum Amount ◽  
In Vitro Toxicity ◽  
Biochemical Profile ◽  
Aerial Parts ◽  
High Cytotoxicity ◽  
Ethyl Acetate Extracts

The present study is intended to reveal the biochemical profile and assess the toxicity and cytotoxic activity of aerial and underground parts of Eragrostis amabilis (L.) Wight. Arn. and Eragrostis pilosa (L.) Beauv. extracts using Brine shrimp lethality bioassay and Dalton’s lymphoma ascites cell (DLA). The maximum amount of protein 31.2 mg/g, amino acid 50.5 mg/g, glucose 62.8 mg/g and Indole acetic acid 29.5 mg/g were found in E. amabilis aerial parts. The maximum amount of total phenolics (218.21 mg/g) and tannins (88.5 mg/g) was observed in ethanolic extracts of E. amabilis aerial parts. The highest value of flavonoids was stated in chloroform extracts of E. amabilis underground parts (544 mg/g). The maximum amount of total phenolics and total tannin was observed in ethyl acetate extracts of E. pilosa aerial parts. The highest amount of flavonoids was found in ethyl acetate extracts of E. pilosa underground parts. The toxicity (LC50 values) of E. amabilis aerial and underground parts were ranged from 0.48 - 1.52 mg/ mL and 0.012 - 1.154 mg/ mL respectively. The LC50 value of E. pilosa aerial and underground parts extract was ranged from 1.089 - 1.904 mg/ mL and 0.038 -1.726 mg/ mL. The high cytotoxicity (ICT50) was observed in ethyl acetate extracts of E. amabilis and E. pilosa.

scholarly journals Evaluation of in vitro antioxidant activity of different extracts of entire plant of Ipomoea pestigridis Linn

2020 ◽  
Vol 11 (1) ◽  
pp. 185-190
Author(s):  
Bheemreddy Thrinitha ◽  
Murali R ◽  
Manichandrika P
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Morning Glory ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Chelating Activity ◽  
Aerial Parts ◽  
Total Antioxidant

Ipomoea pestigridis (Linn) (family Convolvulaceae) is commonly known as “Tiger Foot Morning Glory” in English and locally known as ‘Pulichuvadi’ or ‘Pulichuvadu’ in Malayalam. The current study, aerial parts of different concentrates(Pet.ether, ethyl acetate, and methanol) of I.pestigridis, was evaluated for its in-vitro antioxidant potential by nitric oxide activity, total antioxidant activity, iron chelating activity taking ascorbate & Ethylenediamine tetraacetate as the standard correspondingly. An IC50 value was originated that EA concentrates of I.pestigridis  more efficient in nitric oxide activity, total antioxidant activity, Iron chelating capacity compared methanolic & PE concentrates. The ethyl acetate concentrates of I.pestigridis  & ascorbic acid exhibited antioxidant potential possessing IC50 226µg/ml & 66µg/ml (Nitric oxide). 185µg/ml & 60µg/ml (total antioxidant) , 287µg/ml & 65µg/ml (iron-chelating Activity) respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents.  Invitro antioxidant studies obviously show  EA concentrates of I.pestigridis have better antioxidant activity. These results indicate that aerial parts of methanolic concentrate I.pestigridis  could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

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