scholarly journals A Laboratory Method to Measure Skin Surface Staining by Cigarette Smoke, Tobacco Heating Products and E-Cigarettes

2021 ◽  
Vol 30 (4) ◽  
pp. 158-166
Author(s):  
Annette Dalrymple ◽  
Emma-Jayne Bean ◽  
Jesse Thissen ◽  
Holger Behrsing ◽  
Steven Coburn ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Skin Surface ◽  
Laboratory Method ◽  
Porcine Skin ◽  
Skin Sample ◽  
Side Stream ◽  
Air Control ◽  
And Control ◽  
Skin Samples

Summary Exhaled or side-stream cigarette smoke (CS) may visually stain a consumer's skin over time. Tobacco heating products (THPs) and e-cigarettes (ECs) have reduced staining potential because they do not produce side-stream aerosols and their exhaled aerosols have significantly reduced levels of toxicants, particles and odour. Here we assess discolouration of porcine skin in vitro after exposure to particulate matter (PM) or aerosols from CS (3R4F), two THPs (glo and glo sens) and an EC (iSwitch Maxx). PM was prepared by capturing aerosols on Cambridge filter pads and eluting with dimethyl sulfoxide (DMSO). Abattoir-obtained porcine skin samples were incubated with PM or DMSO control at 37 °C between 0 and 6.0 h. For aerosol assessment, porcine skin samples were exposed to between 50 and 400 puffs of the products, or air control, using a smoking machine. Colour profiles and staining levels of each skin sample were measured at different timepoints and puff thresholds using a spectrophotometer. Staining increased with time and dose, the greatest changes being observed following exposure to aerosols and PM from CS. THP, EC and control values were significantly different from CS after 0.5 h exposure to PM or 50 puffs of aerosols. The minimal staining induced by THPs and EC was comparable to controls. These data suggest that THPs and ECs could offer hygiene benefits to consumers who switch from smoking cigarettes. Further studies are required to assess the longer-term effects of THPs and ECs on skin discoloration. [Contrib. Tob. Nicotine Res. 30 (2021) 158–166]

ULTRASOUND ENHANCED SKIN OPTICAL CLEARING: MICROSTRUCTURAL CHANGES

2010 ◽  
Vol 03 (03) ◽  
pp. 189-194 ◽  
Author(s):  
XIANGQUN XU ◽  
CHAOJIE SUN
Keyword(s):  
Lipid Bilayers ◽  
Ex Vivo ◽  
Skin Surface ◽  
Glycerol Solution ◽  
Porcine Skin ◽  
Intact Skin ◽  
Sem Images ◽  
Optical Clearing ◽  
Better Than

Our previous studies demonstrated the ultrasound-induced skin optical clearing enhancement with topical application of optical clearing agents on in vitro porcine skin and in vivohuman skin. The objective of this study was to investigate the possible mechanisms of the enhanced skin optical clearing by ultrasound medications. Optical clearing effects of ex vivo guinea pig abdomen skin topically applied with 60% glycerol or the combination of 60% glycerol and ultrasound were studied by optical coherence tomography (OCT). Microstructure of skin surface was examined by scanning electron microscopy (SEM). Ultrasound with a frequency of 1 MHz and a power of 0.75 W over a 3-cm probe was simultaneously applied with glycerol solution for 15 min. The combination of 60% glycerol and ultrasound results in a 19% increase in OCT 1/e light penetration depth after 30 min, which is much better than 60% glycerol alone. SEM images demonstrated that changes in skin microstructure due to the tight order of the lipid bilayers in the stratum corneum disrupted and the separation of keratinocytes by the application of ultrasound contribute to the ultrasound-enhanced intact skin optical clearing effects.

scholarly journals Modelling and Control of Corticotropin Permeation from Hydrogels across a Natural Membrane in the Presence of Albumin

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1674
Author(s):  
Wioletta Siemiradzka ◽  
Barbara Dolińska ◽  
Florian Ryszka
Keyword(s):  
Gastrointestinal Tract ◽  
Skin Penetration ◽  
Skin Surface ◽  
Porcine Skin ◽  
Release Process ◽  
Pig Skin ◽  
Semi Solid ◽  
And Control ◽  
Effective Penetration ◽  
Acth Release

(1) Background: Skin is a difficult barrier to overcome, especially for molecules with masses greater than 500 Da. It has been suggested that albumin may contribute to more effective penetration of many therapeutic substances. In this study, an attempt was made to use albumin in semi-solid formulations to increase the skin penetration of another peptide—corticotropin (ACTH). (2) Methods: Hydrogels were prepared at two concentrations: 15 mg/g and 20 mg/g corticotropin, then albumin was added to them in different stoichiometric ratios. The degree of ACTH release from hydrogels, both with and without albumin addition, was investigated. For selected hydrogels the process of corticotropin permeation through a model membrane, i.e., pig skin, was examined. (3) Results: The study of corticotropin release showed that the addition of albumin, depending on its amount, may delay or increase the release process. Similarly, a study of ACTH permeation through porcine skin showed that albumin can delay or increase and accelerate ACTH permeation. (4) Conclusions: Hydrogel, applicated on the skin surface, may prove to be a beneficial and convenient solution for patients. It is an innovative way of application ACTH that bypasses the gastrointestinal tract and may result in increased availability of the peptide and its efficacy.

scholarly journals PENGARUH ASAP ROKOK TERHADAP KUALITAS HIDUP TOTAL PENDERITA RINITIS ALERGI PERSISTEN

2016 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Roy David Sarumpaet ◽  
Mohammad Juffrie ◽  
Suprihati . ◽  
Indwiani Astuti
Keyword(s):  
Quality Of Life ◽  
Allergic Rhinitis ◽  
Cigarette Smoke ◽  
Total Quality ◽  
Treatment Groups ◽  
Side Stream ◽  
Persistent Allergic Rhinitis ◽  
And Control ◽  
The Relationship

ABSTRAK Pendahuluan: Pengaruh asap rokok pada penderita Rinitis Alergi Persisten (RAP) yang prevalensinya semakin meningkat di Indonesia belum mendapat perhatian untuk diteliti, meskipun jumlah penduduk yang merokok semakin bertambah. Tujuan: Menganalisis hubungan asap rokok “side-stream” (SS)  dengan  perubahan kualitas hidup (KH) total penderita Rinitis Alergi Persisten (RAP).  Metode: Penelitian ini adalah dengan desain kasus kontrol, dimana 63  penderita RAP  sedang-berat yang dibuktikan dengan tes alergi dibagi menjadi kelompok perlakuan (32) dan kontrol (31) secara acak. 32 penderita RAP dipaparkan dengan asap rokok (SS) dari 5 batang rokok selama 2 jam dalam  suatu  ruangan. Seluruh responden diminta mengisi kuesioner kualitas hidup dari Juniper’s RQLQ. Hasil: KH total antara kelompok perlakuan dengan kontrol tidak berbeda bermakna. KH total yang terpapar asap rokok setiap hari  berbeda bermakna. Diskusi: Asap rokok yang dipaparkan pada penderita RAP tidak menyebabkan perubahan kualitas hidup.ABSTRACT Introduction: The effect of cigarette smoke on Persistent Allergic Rhinitis patients (RAP) prevalence is increasing in Indonesia. Although the number of people who smoke is increasing yet  it has not received attention for examination. Objective: To analyze the relationship between cigarette smoking "side-stream" (SS) with the change in total quality of life (TQL) among patients with Persistent Allergic Rhinitis (PAR). Method: This study is a case-control design, in which 63 patients with moderate-severe RAP evidenced by allergy tests are divided into treatment groups (32) and control (31) randomly. RAP 32 patients were exposed to cigarette smoke (SS) from 5 cigarettes for 2 hours in a room. All respondents were asked to fill out questionnaires quality of life of Juniper's RQLQ. Results: KH total between treatment groups was not significantly different with control. KH total exposed to secondhand smoke every day significantly different. Discussion: Cigarette smoke described in patients with RAP does not lead to changes in the quality of life.

Dermal Penetration of Ethylene Glycol Through Human Skin In Vitro

2010 ◽  
Vol 29 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Shakil A. Saghir ◽  
Michael J. Bartels ◽  
William M. Snellings
Keyword(s):  
Steady State ◽  
Ethylene Glycol ◽  
Total Radioactivity ◽  
Dermal Absorption ◽  
Skin Sample ◽  
Dermal Penetration ◽  
Steady State Flux ◽  
Flow Through ◽  
Skin Samples

This study was conducted to determine the in vitro dermal absorption of ethylene glycol (EG) through dermatomed human abdominal skin (containing epidermis and dermis), obtained from cadavers within 24 hours of death and kept frozen until processed. Three formulations of EG (neat, 50%, and 10% aqueous solutions) were applied in triplicate to skin samples from 6 donors, and placed in Teflon Bronaugh flow-through diffusion cells. Barrier integrity of each sample was evaluated with 3H-H2O prior to applying EG and only data from samples passing the test were used. A physiological receptor fluid was pumped beneath the skin samples and collected in a fraction collector at predetermined time points through 24 hours. Possible volatilized EG was trapped in a charcoal basket located above each skin sample. Each skin sample was treated with an infinite dose of 500 µL of EG formulation/cm2. At the end of 24 hours, volatilized EG trapped in the headspace was collected, the unabsorbed dose was removed from the skin and the skin was rinsed, tape stripped, and solubilized along with a rinse of the flow-through cells, and total radioactivity was determined. Only a small fraction (≤1%) of the applied EG was absorbed in 24 hours, of which <0.7% penetrated through the skin and ~0.4% remained in the skin. Recovery (mass balance) of the applied EG was between 93% and 99%, which further validated the observed low dermal penetration of EG. The net penetration of the applied EG over 24 hours was concentration proportional, comprising 2.97 ± 0.78, 1.75 ± 0.62, and 0.23 ± 0.12 mg/cm2 of the neat, 50%, and 10% formulations, respectively. The steady-state flux of EG was established between 16 and 24 hours. The mean steady-state flux of EG through dermatomed skin was 217, 129, and 15 µg/cm2h for the neat, 50%, and 10% aqueous formulations, respectively, consistent with concentration-proportional penetration of EG. The steady-state permeation coefficient (Kp) for EG was low, between 1.5 × 10−4 and 2.6 × 10−4 cm/h. These findings demonstrate that EG dermal penetration is expected to be very low and to be slow, indicating very limited systemic or internal dose of EG due to dermal exposure.

Microneedle-Assisted Percutaneous Transport of Magnesium Sulfate

2020 ◽  
Vol 17 (2) ◽  
pp. 140-147
Author(s):  
Karna B. Ghimirey ◽  
Kevin Ita
Keyword(s):  
Confocal Microscopy ◽  
Stratum Corneum ◽  
Magnesium Sulfate ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Magnesium Concentration ◽  
Porcine Skin ◽  
Time Curve ◽  
Student’S T

Objective: In vitro diffusion experiments were performed to assess the permeation of magnesium sulfate across pig skin. Method: The mean thickness of the dermatomed porcine skin was 648 ± 12 µm. Magnesium concentration was measured using inductively coupled plasma-optical emission spectroscopy. Transdermal flux of magnesium sulfate across MN-treated and untreated porcine skin was obtained from the slope of the steady-state linear portion of cumulative amount versus time curve. Results: Statistical analysis of the results was done with Student’s t-test. The transdermal flux of magnesium sulfate across microneedle-treated porcine skin was 134.19 ± 2.4 µg/cm2/h and transdermal flux across untreated porcine skin was 4.64 ± 0.05 µg/cm2/h. Confocal microscopy was used to visualize the microchannels created by a solid microneedle roller (500 µm). Conclusion: From our confocal microscopy studies, it was evident that the 500 μm long microneedles disrupted the stratum corneum and created microchannels measuring 191 ± 37 µm. The increase in transdermal flux across the microneedle-treated skin was statistically significant compared to that of controls, i.e., without the application of microneedles. With the application of microneedles, the transdermal flux of magnesium permeated over 12 h was approximately 33-fold higher in comparison to passive diffusion across an intact stratum corneum.

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

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