scholarly journals The optimization of extraction process of white mulberry leaves and the characteristic bioactive properties its powder extract

2019 ◽  
Vol 65 (1) ◽  
pp. 12-19
Author(s):  
Krzysztof Przygoński ◽  
Elżbieta Wojtowicz

Summary Introduction: Powder extracts from white mulberry leaves (Morus alba L.) are desirable due to their simplicity of use. Powders intended for use in food and dietary supplements can be obtained by various methods, including water extraction and spray drying. Objective: The aim of the work was to optimize the aqueous process of extraction of white mulberry leaves and characterization of bioactive properties of the obtained extract powder. Methods: The DNJ and flavonoids (quercetin, kaempferol) contents and were analysed by high performance liquid chromatography (HPLC). Total phenolic contents were determined using the Folin-Ciocalteu phenol reagent and antioxidant activity by ABTS and DPPH assays. Results: The mulberry leaf extraction process has been optimized. The obtained powdered mulberry leaf extract proved to be a good source of bioactive compounds. Characteristic phenolic compounds detected in mulberry leaves and their powder extract were quercetin and kaempferol. The sum of polyphenols was 10.9 mg GAE/g dry matter in the raw material, whereas in the powder extract 42.6 mg GAE/g dry matter. In addition, it was found that the obtained powdered extract is characterized by a five-fold higher, in comparison to the raw material, the content of antioxidant activity measured by ABTS and DPPH tests. The content of 1-deoxynojirimycin (DNJ) in the powder extract was nearly four times higher than in dry mulberry leaves. Conclusion: The optimized process of water extraction of white mulberry leaves allows to keep valuable bioactive components and to obtain their high concentration.

2017 ◽  
Vol 14 (1) ◽  
pp. 1-9
Author(s):  
THERESIA AGNIEST PRICILLA VITANTI ◽  
KAWIJI KAWIJI ◽  
EDHI NURHARTADI

Vitanti TAP, Kawiji, Edhi N. 2012. Effect of extraction method on Curcuma xanthorrhiza oleoresin using solar dryer to concentration of curcuminoid, total phenol, and antioxidant activity. Biofarmasi 14: 1-9. Curcuma (Curcuma xanthorrhiza Roxb.) is a type of drug plant that has high enough capacity of production in Indonesia. Generally, commerced in the form of fresh curcuma or processed product as simple as simplicia and curcuma powder. Processed products that could be developed is curcuma oleoresin. It is a mixture of essential oils and resins obtained from extraction process of curcuma powder using an organic solvent. Oleoresin has the same flavor and aroma to the extracted material. Due to these characteristics, it is used as a flavor and food coloring, other than as a raw material in pharmaceutical industry. In addition, it also contains active compounds which can support the utilization of drug and food industries. This study aims to determine whether the size of the powder, powdered curcuma immersion time, and interactions between them that can be influenced the content of curcuminoids, total phenol and antioxidant activity of curcuma oleoresin. Selection of solar dryers in the drying process is based on previously studied that compare the natural drying technique with a solar dryer, and the best results of those studies are shown in the solar dryer. This research using completely randomized design with two factors: the size variation of curcuma powder (60, 80 and 100 mesh) and immersion time variation (extraction) of curcuma powder (12, 24 and 36 hours). The results showed that the powder size of curcuma and immersion time has an effect on curcuminoid content, total phenol and antioxidant activity of curcuma oleoresin. However, there are no interaction between both factors. That is, the size and the immersion time of curcuma powder do not affect each other on the content of curcuminoid, total phenol and activity of antioxidant.


Author(s):  
Milena Polumackanycz ◽  
Marek Wesolowski ◽  
Agnieszka Viapiana

AbstractBioactivity of mulberry has been widely described, but mostly related to its fruits, while studies with leaves are scarce. Herein, in this study leaves of two mulberry species (Morus alba L. and Morus nigra L.) were characterized, compared, and evaluated in terms of their phenolic composition and antioxidant activity. Aiming to valorize mulberry leaves extracts which can be included in modern diet four different extracts (infusions, decoctions, tinctures and hydromethanolic) were examined. The chemical characterization was done by quantifying total phenolics (TPC), flavonoids (TFC) and phenolic acids (TPAC) content, L( +)ascorbic acid (ASA) and individual phenolic compounds in the extracts by HPLC technique. Moreover, DPPH and FRAP assays were used to assess the antioxidant activity of white and black mulberry leaves. Findings of these studies revealed that black mulberry leaves were richer in TFC and TPAC, while white mulberry leaves contained higher levels of individual phenolic compounds in water extracts. Gallic acid was found in the highest concentrations in two mulberry leave extracts. Correlation analysis showed strong relationships between antioxidant activity and TPC and TFC. This fact suggests the crucial role of phenolic compounds as antioxidant agents in white and black mulberry leaves. The results obtained in this study demonstrate that leaves of both white and black mulberry can be used as a valuable source of phenolic compounds with bioactive potential, which can be applied in the food sector, as foods and as promising source of natural ingredients.


2019 ◽  
Vol 78 ◽  
pp. 02014 ◽  
Author(s):  
Xiaoli Zhou ◽  
Guangxu Zhu ◽  
Yansheng Yang ◽  
Bin Du ◽  
Dong Lin

In this experiment, at first the roxburgh rose juice was extracted and then roxburgh rose residue was taken as raw material. We have used the enzyme assisted method to study the extraction process of polysaccharides from roxburgh rose pomace. The effects of mesh number, the concentration of the enzyme, temperature and time, pH and solid-liquid ratio on the polysaccharides yield were explored by single factor experiments. And then orthogonal experiment was designed to study the optimal techniques on extracting of polysaccharides from pomace. The in vitro antioxidant activity of the obtained polysaccharides was studied. The results showed that the optimal condition of extracting polysaccharides was as follows: enzyme concentration 2.5%, enzymatic hydrolysis at 60° for 40 min, pH 4.0, mesh number were 100 and solidliquid ratio was 1:25 based on the ratio of cellulase and pectinase was 2: 1. The average polysaccharides yield of enzymatic extraction method reached (4.79±0.07) % under the optimal condition. The antioxidant activity assays in vitro revealed that polysaccharides from roxburgh rose pomace can be used as natural antioxidants in functional foods and pharmaceutical industries.


2011 ◽  
Vol 43 (5) ◽  
pp. 995-999 ◽  
Author(s):  
Jaime Salinas-Chavira ◽  
Omar Castillo-Martínez ◽  
J. Efren Ramirez-Bribiesca ◽  
Miguel Mellado

2021 ◽  
Vol 17 (2) ◽  
pp. 40-47
Author(s):  
E. V. Lisovaya ◽  
E. P. Victorova ◽  
A. V. Sverdlichenko ◽  
N. N. Kornen

It is known that enterprises processing vegetables and fruits generate secondary resources (waste), in particular, pomace, in significant volumes. The most valuable, in terms of the composition and content of biologically active macro- and micronutrients, is tomato pomace. Tomato pomace is a valuable source of natural carotenoids, including lycopene, which exhibits high antioxidant activity. The article provides an overview of scientific research aimed at developing methods for extracting carotenoids, including lycopene, from recycled tomato processing resources. It has been shown that, in general, when extracting carotenoids, including lycopene, from secondary resources of tomato processing, various methods of drying and grinding the raw material are used, and then biologically active substances are extracted. It should be noted that the method of pretreatment of the feedstock, the nature of the solvent, the temperature and duration of the extraction process have a significant effect on the antioxidant activity and bioavailability of carotenoids obtained as a result of extraction. It is shown that the use of a pulsed electric field, treatment with enzymes and ultrasonic treatment of tomato pomace allow to intensify the process of extraction of carotenoids and maximally preserve their antioxidant properties. Thus, it can be concluded that tomato pomace is a valuable raw material for obtaining extracts of carotenoids and, first of all, lycopene, and the development of effec- tive modes of the extraction process that ensure the maximum manifestation of antioxidant properties and the bioavailability of the extracted substance is an urgent task.


2020 ◽  
Vol 9 (10) ◽  
pp. e9939109273
Author(s):  
Felipe da Silva Veloso ◽  
Eliane Colla ◽  
Aziza Kamal Genena

It is well known that vegetable residues from the food industry can be used as a possible source for the extraction of compounds with antioxidant activity; in the case of mango, approximately 40 to 60% of the total mass of the fruit is considered as residue after processing. This work focused on the optimization of the extraction process of compounds with antioxidant activity from the rind of Tommy Atkins mango. Initially a Fractional Factorial Design 25-1 (FFD) was applied to study the effect of five variables on the extraction process, namely: extraction time (min), percentage of ethanol in aqueous solution (%), pH, dry/solvent mango peel ratio (g/mL) and ultrasound power range (%). The variables extraction time and dry/solvent mango peel ratio were selected, which were evaluated sequentially by the application of a Central Composite Rotatable Design (CCRD) to determine the conditions of maximum extraction of antioxidant compounds by the response surface analysis. The ABTS•+ and Folin-Ciocalteu methods were used for the quantification of the antioxidant activity.  The maximum extraction occurred with the use of water, extraction time of 30 min, dry mango peel/solvent ratio of g/mL (1:100), at natural pH of the mixture (pH 4.6 ± 0.20) and sonication amplitude at 50%. It was possible to develop an extraction process of compounds with antioxidant activity from the mango peel, in order to maximize yield through the use of non-toxic solvents and using an agro-industrial residue as raw material.


2021 ◽  
Vol 12 ◽  
Author(s):  
Lei Hu ◽  
Cheng Wang ◽  
Xiang Guo ◽  
Dekui Chen ◽  
Wei Zhou ◽  
...  

In recent years, various mulberry leaf dishes have gradually gained in popularity. The harvesting period of mulberry leaves and the preparation of dried samples are critical for the retention of flavonoid content and activity. In this study, changes in flavonoid levels in mulberry leaves during their growth period (3–6 months), and the effects of four different drying methods [sun drying (SD), air drying (AD), oven drying (OD), and freeze drying (FD)] on flavonoid accumulation and antioxidant capacity were determined. The results showed that mulberry leaves grown for 6 months had higher levels of flavonoids, and different drying methods could significantly affect the flavonoid levels and antioxidant capacity of the leaves. Air drying and FD were the best methods in terms of retaining the antioxidant activity of flavonoids, although AD had lower operating costs than FD in the production process. Therefore, to obtain a high flavonoid content and maximum antioxidant activity, AD is recommended for mulberry leaves.


2012 ◽  
Vol 8 (1) ◽  
pp. 123-132 ◽  
Author(s):  
Zsuzsanna Horváth ◽  
Béla Marosvölgyi ◽  
Christine Idler ◽  
Ralf Pecenka ◽  
Hannes Lenz

Abstract - There are several problems in storing wood chips freshly harvested from short rotation plantations, which result in quality losses as well as in dry matter and energy losses. The factors influencing the degradation of raw material are examined in this paper with special focus on fungal development. An excessive growth of fungi is connected to dry matter losses and also to an increased health risk during raw material handling. The following factors were measured during 6 months storage of poplar wood chips depending on particle size: box temperature, moisture content, pH-value, appearance of fungi in the storage and the concentration of fungal particles in the air. The results show a close connection between particle size, temperature and attack of fungi. During the storage mesophilic and termophilic species of the genera Alternaria, Aspergillus, Cladosporium, Mucor and Penicillium appeared. The concentration of fungal particles is the highest for fine chips and decreases in bigger particles. There was a special focus on the investigation of the properties of coarse chips (G 50), which represent a good compromise between handling, storage losses and health risk due to fungal development.


2012 ◽  
Vol 41 (5) ◽  
pp. 706-713 ◽  
Author(s):  
Sang-Il Lee ◽  
Ye-Kyung Lee ◽  
Jong-Keun Choi ◽  
Seung-Hwan Yang ◽  
In-Ae Lee ◽  
...  

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