scholarly journals Current status of porcine epidemic diarrhoea (PED) in European pigs

2019 ◽  
Vol 63 (4) ◽  
pp. 465-470
Author(s):  
Marta Antas ◽  
Grzegorz Woźniakowski
Keyword(s):  
Rna Virus ◽  
Acute Diarrhoea ◽  
Current Status ◽  
Economic Losses ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
The Us ◽  
The Usa ◽  
Suckling Piglets ◽  
Farming Industry

Abstract Porcine epidemic diarrhoea (PED) is a highly contagious and devastating enteric disease of pigs caused by porcine epidemic diarrhoea virus (PEDV), an enveloped, single-stranded RNA virus belonging to the Alphacoronavirus genus of the Coronaviridae family. The disease is clinically similar to other forms of porcine gastroenteritis. Pigs are the only known host of the disease, and the occurrence of PED in wild boars is unknown. The virus causes acute diarrhoea, vomiting, dehydration, and high mortality in suckling piglets reaching 100%. Heavy economic losses in the pig-farming industry were sustained in the USA between 2013 and 2015 when PEDV spread very quickly and resulted in epidemics. The loss in the US pig industry has been estimated at almost seven million pigs. The purpose of this review is a description of the current status of porcine epidemic diarrhoea in European pigs and the risk presented by the introduction of PEDV to Poland in comparison to the epidemics in the USA.

scholarly journals Molecular characterization of porcine epidemic diarrhoea virus (PEDV) in Poland reveals the presence of swine enteric coronavirus (SeCoV) sequence in S gene

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258318
Author(s):  
Marta Antas ◽  
Monika Olech ◽  
Anna Szczotka-Bochniarz
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rna Virus ◽  
Economic Losses ◽  
High Morbidity ◽  
Antigenic Analysis ◽  
S Gene ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus

Porcine epidemic diarrhoea (PED) is a highly contagious enteric viral disease of pigs with a high morbidity and mortality rate, which ultimately results in huge economic losses in the pig production sector. The etiological agent of this disease is the porcine epidemic diarrhoea virus (PEDV) which is an enveloped, positive single-stranded RNA virus. The aim of this study was to perform molecular characterization of PEDV to identify the strains circulating in Poland. In this study, 662 faecal samples from 2015 to 2021 were tested with reverse transcription quantitative real-time PCR (RT-qPCR) and the results showed that 3.8% of the tested samples revealed a positive result for PEDV. A phylogenetic analysis of the complete genome and complete S gene sequences showed that Polish PEDV strains belonged to the G1b (S-INDEL) subgroup and were closely related to the European PEDV strains isolated from 2014 to 2019. Furthermore, RDP4 analysis revealed that the Polish PEDV strains harboured a recombinant fragment of ~400 nt in the 5’ end of S gene with PEDV and swine enteric coronavirus (SeCoV) being the major and minor parents, respectively. Antigenic analysis showed that the aa sequences of neutralizing epitopes were conserved among the Polish PEDV strains. Only one strain, #0100/5P, had a unique substitution in the COE epitope. However, Polish PEDV strains showed several substitutions, especially in the COE antigen, as compared to the classical strain CV777. To the best of our knowledge, this is the first report concerning the molecular characterization of porcine epidemic diarrhoea virus strains, as well as the first phylogenetic analysis for PEDV in Poland.

scholarly journals Frequency of diarrhoea-associated viruses in swine of various ages in Hungary

2019 ◽  
Vol 67 (1) ◽  
pp. 140-150 ◽  
Author(s):  
Anna Valkó ◽  
András Marosi ◽  
Attila Cságola ◽  
Rózsa Farkas ◽  
Zsuzsanna Rónai ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Real Time Pcr ◽  
Economic Losses ◽  
Viral Diseases ◽  
Weaned Pigs ◽  
Pork Production ◽  
Faecal Samples ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
Significant Difference

Enteric viral diseases of swine are one of the most frequent disorders causing huge economic losses in pork production. After the reappearance of an emerging enteropathogen, porcine epidemic diarrhoea virus (PEDV) in Hungary in 2016, an extensive survey was initiated in an attempt to identify diarrhoea-related porcine viruses, including adeno-, astro-, boca-, calici-, circo-, corona-, kobu-, rota- and Torque teno viruses. A total of 384 faecal samples collected during a twoyear period from diarrhoeic and asymptomatic pigs of various ages in 17 farms were screened by conventional and real-time PCR methods. Half of the samples contained at least one examined virus with the dominance of kobuvirus (55.1%) followed by bocaviruses (33.2%) and rotavirus groups A and C together (20.9%), while coronaviruses including PEDV were not found in this set of samples. Statistical analysis showed a highly significant difference (P < 0.0001) in the frequency of single infections compared to mixed ones with the exception of weaned pigs, in which group additionally most viruses were detected. The results of this study suggest that the complexity of this disease may vary with age, which makes the prevention of diarrhoea a challenge, especially in weaned pigs.

scholarly journals Rotavirus C: prevalence in suckling piglets and development of virus-like particles to assess the influence of maternal immunity on the disease development

2019 ◽  
Vol 50 (1) ◽  
Author(s):  
Juliet Chepngeno ◽  
Annika Diaz ◽  
Francine C. Paim ◽  
Linda J. Saif ◽  
Anastasia N. Vlasova
Keyword(s):  
Cell Culture ◽  
Diarrheal Disease ◽  
Economic Losses ◽  
Disease Development ◽  
Fecal Samples ◽  
Virus Like Particles ◽  
Maternal Immunity ◽  
The Us ◽  
Suckling Piglets ◽  
Major Impediment

Abstract Rotavirus C (RVC) has been detected increasingly in humans and swine in different countries, including the US. It is associated with significant economic losses due to diarrheal disease in nursing piglets. In this study we aimed: (1) to determine the prevalence of RVC in healthy and diarrheic suckling piglets on US farms; and (2) to evaluate if maternal antibody (Ab) levels were associated with protection of newborn suckling piglets against RVC. There was a significantly higher prevalence (p = 0.0002) of litters with diarrhea born to gilts compared with those born to multiparous sows. Of 113 nursing piglet fecal samples tested, 76.1% were RVC RNA positive. Fecal RVC RNA was detected in significantly (p = 0.0419) higher quantities and more frequently in piglets with diarrhea compared with healthy ones (82.5 vs. 69.9%). With the exception of the historic strain Cowden (G1 genotype), field RVC strains do not replicate in cell culture, which is a major impediment for studying RVC pathogenesis and immunity. To circumvent this, we generated RVC virus-like particles (VLPs) for Cowden (G1), RV0104 (G3) and RV0143 (G6) and used them as antigens in ELISA to detect swine RVC Abs in serum and milk from the sows. Using RVC-VLP Ab ELISA we demonstrated that sows with diarrheic litters had significantly lower RVC IgA and IgG Ab titers in milk compared to those with healthy litters. Thus, our data suggest that insufficient lactogenic protection provided by gilts plays a key role in the development of and the increased prevalence of clinical RVC disease.

Chernobyl coverage: how the US media treated the nuclear industry

1992 ◽  
Vol 1 (3) ◽  
pp. 305-323 ◽  
Author(s):  
Sharon M. Friedman ◽  
Carole M. Gorney ◽  
Brenda P. Egolf
Keyword(s):  
Chernobyl Accident ◽  
Nuclear Power ◽  
Western Europe ◽  
Nuclear Industry ◽  
Current Status ◽  
Background Information ◽  
The Us ◽  
The Usa ◽  
History Of ◽  
Level Of Understanding

This study attempted to uncover whether enough background information about nuclear power and the nuclear industries in the USA, USSR and Eastern and Western Europe had been included during the first two weeks of US coverage of the Chernobyl accident so that Americans would not be misled in their understanding of and attitudes toward nuclear power in general. It also sought to determine if reporters took advantage of the Chernobyl accident to attack nuclear technology or the nuclear industry in general. Coverage was analysed in five US newspapers and on the evening newscasts of the three major US television networks. Despite heavy coverage of the accident, no more than 25% of the coverage was devoted to information on safety records, history of accidents and current status of nuclear industries. Not enough information was provided to help the public's level of understanding of nuclear power or to put the Chernobyl accident in context. However, articles and newscasts generally balanced use of pro- and anti-nuclear statements, and did not include excessive amounts of fear-inducing and negative information.

scholarly journals Epizootiological and diagnostic significance of porcine reproductive and respiratory syndrome control

2002 ◽  
Vol 56 (1-2) ◽  
pp. 21-31 ◽  
Author(s):  
Biljana Radojicic ◽  
Bosiljka Djuricic ◽  
M. Gagrcn
Keyword(s):  
Clinical Picture ◽  
Bacterial Infections ◽  
Rna Virus ◽  
Economic Loss ◽  
Viral Disease ◽  
Economic Losses ◽  
Diagnostic Significance ◽  
Time Period ◽  
Suckling Piglets ◽  
Almost All

The porcine reproductive and respiratory syndrome (PRRS) is a new viral disease in swine, designated exclusively under the acronym PRRS by the European Commission in 1991. The cause of this disease was isolated and determined in 1991 at the Lelystad Institute in The Netherlands as Lelystad aretrivirus. The PRRSV is an RNA virus of the order Nidovirales, the family Arteriviridae, the genus Arterivirus (Cavanaugh, 1997). Different genomic and pheriotypic varieties of the virus are significant. It is replicated in macrophages, it induces permanent viraemia, causes the creation of antibodies, and leads to persistent and latent infections. It is isolated from tonsil tissue, alveolar macrophages, the uterus, and fetal homogenate composed of different tissues (Wills et al., 1997). All production categories of swine can contract PRRS, but pregnant sows, suckling piglets and fattening swine are considered endangered categories. Morbidity and mortapty is between 8-80%, which also depends on the animal category. Economic damages are substantial when one considers the high percentage of still-born piglets, mummified fetuses and suckling piglets. Irregular successive cycles in sows are also expressed. In fattening swine, in addition to a respiratory form of the clinical picture, the time period until animals reach abattoir weight is extended even up to 30 days, which is also a considerable economic loss. Costs of treating possible secondary bacterial infections, diagnostics and immunoprophylaxis are not negligible. The OIE placed PRRS on the B list in 1992 as a contagious disease of swine which incurs economic losses in almost all countries of the world. Diagnosis is made by isolating and determining the virus and/or by serodiagnostics (ELISA and PCR). Certain countries have already made up protocols for the implementation of constant diagnostics and suggested eradication measures (Dee S.A. et al., 2000). In our country, the first clinical cases of PRRS were recorded in Herceg Novi in 1998 (Radojicic Biljana et al., 2002). It is our opinion that the implementation of PRRS diagnostics must begin in our country as well, especially since the disease has a clinical picture which is similar to swine plague, so that wrong diagnoses are possible.

scholarly journals Isolation and characterisation of porcine epidemic diarrhoea virus in Hungary – Short communication

2019 ◽  
Vol 67 (2) ◽  
pp. 307-313 ◽  
Author(s):  
Anna Valkó ◽  
Ervin Albert ◽  
Attila Cságola ◽  
Tünde Varga ◽  
Krisztián Kiss ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Sequence Alignment ◽  
Short Communication ◽  
Whole Genome Sequence ◽  
Economic Losses ◽  
Whole Genome ◽  
S Gene ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus

Porcine epidemic diarrhoea virus (PEDV) is an emerging enteropathogen, causing great economic losses in the pig industry. After many years of quiescence, PEDV was detected in Hungary in 2016 with a recombination in its S gene. In order to determine the extent of this change, an attempt was made to isolate the recombinant PEDV. This study was extended with a variety of samples collected from three separate farms with newly identified PEDV in 2018. The recombinant PEDV from 2016 was isolated successfully along with three viruses from 2018, and one isolate from the new cases was used for whole genome determination. Whole genome sequence alignment revealed the highest identity with recombinant Hungarian and Slovenian PEDV within the low-pathogenic European viruses. This suggests that these recombinant PEDV are circulating in this area and may spread to other parts of the continent.

scholarly journals Web of Science-Based Green Infrastructure: A Bibliometric Analysis in CiteSpace

Land ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 711
Author(s):  
Huamei Shao ◽  
Gunwoo Kim ◽  
Qing Li ◽  
Galen Newman
Keyword(s):  
Bibliometric Analysis ◽  
Green Infrastructure ◽  
Environmental Protection Agency ◽  
Environmental Science ◽  
Web Of Science ◽  
Urban Forestry ◽  
Current Status ◽  
Urban Greening ◽  
The Us ◽  
The Usa

Many cities worldwide are using re-greening strategies to help reverse urbanization patterns that aggravate environmental issues. Green infrastructure (GI) has become a significant and effective strategy to address environmental problems. To better understand GI, this study uses CiteSpace to analyze 5420 published papers in the field of GI on the Web of Science database from 1990–2020. This bibliometric analysis will help new scholars and researchers to better understand the current status and trends in GI research, as well as identify further research needed in the field. This study evaluated research on GI trends according to publication amounts, keywords, journals, disciplines, countries, institutions, and authors. Results show that, first, GI research has experienced rapid growth since 2014. Second, GI, ecosystem services, and city are the top three keywords related to GI research, with green roof as the keyword with the strongest linkage. Third, Sustainability, Urban Forestry and Urban Greening, and Landscape and Urban Planning are the top three journals publishing GI research. Fourth, the top three disciplines researching GI are environmental science, engineering, and science and technology. Fifth, the USA is the top ranked country in terms of the number of published GI-related papers (1514 papers), followed by China (730 papers) and England (546 papers). Sixth, the US Environmental Protection Agency (84 papers) is the top institution in terms of publications, followed by the Chinese Academy of Science (83 papers) and the Swedish University of Agriculture (66 papers). Finally, D. Haase has the most published articles (29 papers), followed by S. Pauleit (28 papers) and P. Angelstam (26 papers). These findings indicate that GI has developed significantly in the last 30 years, with a high probability for increased growth in the future.

scholarly journals Porcine enteric coronavirus infections in wild boar in Poland – a pilot study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marta Antas ◽  
Monika Olech ◽  
Anna Szczotka-Bochniarz
Keyword(s):  
Economic Losses ◽  
Parasitic Infections ◽  
Rt Pcr ◽  
Hunting Season ◽  
Blood Samples ◽  
Wild Boars ◽  
Faecal Samples ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
Provinces Of Poland

Abstract Introduction Porcine epidemic diarrhoea virus (PEDV) of the Coronaviridae family causes significant economic losses in the pig industry worldwide. Wild boars contribute to the transmission of different viral, bacterial and parasitic infections to livestock animals and humans. However, their role in the maintenance and transmission of PEDV has not been established. Material and Methods In this study, blood and faecal samples from 157 wild boars were collected from 14 provinces of Poland during the 2017–2018 hunting season. RNA was extracted from the faecal homogenate supernatant and subjected to quantitative RT-PCR (RT-qPCR), while clotted blood samples were used for detection of antibodies against PEDV by ELISA. Results Five blood samples (3.2%) were seropositive in ELISA, while none of the faecal samples were found positive using RT-qPCR assays. Conclusion The results of this analysis indicate the need for additional studies incorporating a larger number of samples and preferably comparing different serological methods, to confirm whether wild boars in Poland act as PEDV reservoirs.

scholarly journals Preparation and characterisation of monoclonal antibodies against the N protein of the SHpd/2012 strain of porcine epidemic diarrhoea virus

2018 ◽  
Vol 63 (No. 10) ◽  
pp. 468-475 ◽  
Author(s):  
SJ Ding ◽  
YR Luo ◽  
ST Zhou ◽  
C. Xie ◽  
K. Wang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Western Blotting ◽  
Test Strip ◽  
Diagnostic Methods ◽  
Economic Losses ◽  
Hybridoma Cells ◽  
N Protein ◽  
Porcine Epidemic Diarrhoea Virus ◽  
Diarrhoea Virus ◽  
And Control

Porcine epidemic diarrhoea is caused by the porcine epidemic diarrhoea virus, and is a highly contagious disease which affects the intestines of new-born piglets resulting in intense diarrhoea. Historically, the virus has caused enormous economic losses in the pig industry. In particular, the emergence of new epidemic strains means there is a pressing need for prevention and control of the disease. Owing to the specificity of the monoclonal antibodies now available, study of the pathogenesis, immune mechanisms and new diagnostic methods can be performed. In this study, 13 strains of positive hybridoma cells were prepared by immunising mice with purified whole porcine epidemic diarrhoea virus, and analysis was performed using ELISA and Western blotting. Three cell strains specifically recognised the porcine epidemic diarrhoea virus nucleocapsid protein (N protein). In this study, we report the characterisation of effective tools for the establishment of porcine epidemic diarrhoea virus diagnostic methods and we have specifically generated primary antibodies for ELISA, IFA, test strip and Western blotting.

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