Biochar changes in soil based on quantitative and qualitative humus compounds parameters

Abstract Due to the indisputable significance of humus in many biochemical processes as well as its increasing deficit particularly in light soils, alternative sources of substrates for the reproduction of this constituent should be sought. The aim of this study was to evaluate the effect of the addition of wheat straw and wheat straw biochar (in four rates) on quantitative and qualitative humus parameters. The following properties were determined in soil: pH, organic carbon, total nitrogen, humic and fulvic acids, carbon in the extract, non-hydrolysing carbon and spectrophotometric indexes for solution of humic acids including A2/6, A2/4, A4/6. After applying 1% and 2% additions of biochar to the soil, the Corg soil content significantly increased compared to the same doses of thermally unconverted straw. After 254 days of incubation, the addition of biochar to soil at higher doses, decreased the share of humic acid carbon (CHA, CFA) in the Corg content compared to treatments without organic additions and WS treatment. The nonhydrolysing carbon soil content was significantly increased by treatments with 1% and 2% additions of WSB, which indicates greater stabilisation of humus compounds and, at the same time, lower CO2 emission. Soil humic acids amended by treatment with biochar, especially at 1% and 2% doses, were characterised by lower A2/6 and A2/4 ratios. Recognition of changes that may occur in the quantitative and qualitative composition of soil humus after the application of biochar may in the future be helpful information for determining appropriate biochar dose.

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EFFECT OF HUMIC AND FULVIC ACIDS ON MICROBIAL GROWTH

10.46793/iccbi21.137r ◽

2021 ◽

Author(s):

Milanka Radulovic ◽

◽

Svetlana Mitrovski

Keyword(s):

Humic Acid ◽

Humic Substances ◽

Minimal Medium ◽

Microbial Growth ◽

Growth Curves ◽

Fulvic Acids ◽

Humic And Fulvic Acids ◽

Staphyloccocus Aureus ◽

Low Ionic Strength ◽

Strength Medium

Peat is a natural substrate for growth of microorganisms because it is rich in compounds that microorganisms can use as sources of carbon, nitrogen and growth factors. Peat originating from Vlasina lake in Eastern Serbia is especially rich in organic matter. The content of humic substances (humic acid, fulvic acid and humine) is almost twice that found in other peat-rich regions of similar origin and geochemical age. Humic and fluvic acids are known to promote microbial growth. In this work, humic and fulvic acids were first extracted from Vlasina lake peat and then added to minimal medium (synthetic, low ionic strength medium). The humic substances were added separately and combined in a 1:1 ratio by mass to study their individual and combined effect on microbial growth of Escherichia coli ATCC 25922 (Gr–), Staphyloccocus aureus (Gr+) i Aureobasidium pullulans, strain CH-1. The microbial growth was measured microspectrophotometrically over a 24-hour period and growth curves were obtained for a range of acid concentrations between 25 µg cm-3 and 100 µg cm-3. It was found that both humic and fulvic acids promote the growth of all three microorganisms by up to a maximum of 40%-80% the extent of which varied with the concentration of the acid and the identity of the microorganism. In general, humic acid was found to result in higher microbial growth (at highest concentrations, up to ~80% for all three microbial species).

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THE EFFECTIVENESS OF COMPOST, HUMIC ACID AND PURE FULVATE ON IMPROVEMENT OF ULTISOL SOIL CHEMICAL PROPERTIES

EPRA International Journal of Multidisciplinary Research (IJMR) ◽

10.36713/epra6996 ◽

2021 ◽

pp. 247-254

Author(s):

Resman ◽

Sahta Ginting ◽

Muhammad Tufaila ◽

Fransiscus Suramas Rembon ◽

Halim

Keyword(s):

Humic Acid ◽

Cation Exchange ◽

Cation Exchange Capacity ◽

Chemical Properties ◽

Fulvic Acids ◽

Exchange Capacity ◽

Humic And Fulvic Acids ◽

Total N ◽

Organic C ◽

Exchangeable Al

The research aimed to determine the effectiveness of compost containing humic and fulvic acids, and pure humic and fulvic acids in increasing of Ultisol soil chemical properties. The research design used a randomized block design (RBD), consisting of 10 treatments, namely K0: 0 g polybag-1, KO1: 500 g polybag-1, KO2: 500 g polybag-1, KO3: 500 g polybag-1, KO4: 500 g polybag-1, KO5: 500 g polybag-1, KO6: 500 g polybag-1, KO7: 500 g of polybags-1, H: 50 g of polybag-1, A: 500 g polybag-1. Each treatment was repeated three times and obtained 30 treatment units. The results showed that pH H2O (K0: 4.49, KO1: 5.64, KO2: 5.47, KO3: 5.43, KO4: 5.51, KO5: 5.39, KO6: 5.48, KO7: 6.17, H: 5.06, F: 5.15), total-N (%) (K0: 0.13, KO1: 0.17, KO2: 0.18, KO3: 0.30, KO4: 0.25, KO5: 0.24, KO6: 0.29, KO7: 0.36, H: 0.16, F: 0.14), organic-C (%) (K0: 1.85, KO1; 2.30, KO2: 2.24, KO3: 2.33, KO4: 2.62, KO5: 2.25, KO6: 2.27, KO7: 2.95, H: 2.32, F: 2.26) , available-P (%) (K0: 2.75, KO1: 3.24, KO2: 3.16, KO3: 3.27, KO4: 3.57, KO5: 3.31, KO6: 3.37, KO7: 3.89, H: 3.10, F: 3.12), exchangeable-Al (me100g-1) (K0: 2.51, KO1: 2.11, KO2: 2.13, KO3: 2.15, KO4: 1.88, KO5: 2.14, KO6: 2.12, KO7: 1.75, H: 2.16, F: 2.17), base saturation (%) (K0: 30.91, KO1: 63.48, KO2: 52.63, KO3: 53.76, KO4: 56.13, KO5: 54.96, KO6: 56.71, KO7: 65.53, H: 39.11, F: 42.76), cation exchange capacity (me100g-1) (K0: 12.76, KO1: 15.64, KO2: 14.86, KO3: 14.35, KO4: 14.13, KO5: 15.01, KO6: 15.50, KO7: 17.94, H: 14.19, F: 13.73). The combined compost treatment of three types of organic matter (Imperata cylindrica + Rice straw + Glincidia sepium) is more effective in increasing the pH, H2O as 37.42%, total-N as 176.92%, Organic-C as 59.46%, available-P as 41.45%, base saturation as 65.53%, cation exchange capacity as 17.94% and exchangeable -Al, Alreduction as 30.28% of ultisol soil. KEY WORDS: compost, humic acid, fulvate, soil chemical, ultisol

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Development of methods for the separation and characterization of natural organic matter in dam water

10.51415/10321/1182 ◽

2014 ◽

Author(s):

◽

Pinkie Sobantu

Keyword(s):

Molecular Weight ◽

Humic Acid ◽

Fulvic Acid ◽

Humic Substances ◽

Water Samples ◽

Fulvic Acids ◽

Hydroxyl Groups ◽

Humic And Fulvic Acids ◽

Vaal Dam

This project arose out the need for a simple method to analyse NOM on a routine basis. Water samples were obtained from the Vaal dam, which is one of the dams used by a hydroelectric power station. Analysis was preceded by separation of NOM into the humic and non-humic portions. The humic portion was separated into two fractions by employing a non-ionic resin (DAX-8) to separate humic acid from fulvic acid. High performance size exclusion chromatography (HPSEC), equipped with an Ultraviolet( UV) detector and an Evaporative Light Scattering (ELS) detector connected in series, was used to obtain molecular weight distribution information and the concentration levels of the two acids. Mixed standards of polyethylene oxide/glycol were employed to calibrate the selected column. Suwanee River humic acid standard was used as a certified reference material. The molecular weight distributions (MWDs) of the isolated fractions of humic and fulvic acids were determined with ELSD detection as weight-average (Mw), number-average (Mn) and polydispersity (ρ) of individual NOM fractions. The Mw/Mn ratio was found to be less than 1.5 in all the fractions, indicating that they have a low and narrow size fraction. An increase in Mn and Mw values, with increasing wavelength for all three humic substances (HS) examined was observed. The HS, isolated from the dam water, was found to be about the same molecular weight as the International Humic Acid Standard (IIHSS). For the fulvic acid standard, the molecular weight was estimated to be around 7500 Da. Characterization of NOM was done to assist in the identification of the species present in the water. FTIR-ATR was used to as a characterization tool to identify the functional groups in the structure of the humic and fulvic acid respectively present in the Vaal Dam. Analysis of the infrared (IR) spectra indicated that the humic acids of the Vaal dam have phenolic hydroxyl groups, hydroxyl groups, conjugated double bond of aromatic family (C=C), and free carboxyl groups. The isolation method has proved to be applicable and reliable for dam water samples and showed to successfully separate the humic substances from water and further separate the humic substances into its hydrophobic acids, namely, humic and fulvic acids. It can be concluded that the Eskom Vaal dam composes of humic substance which shows that the technique alone gives a very good indication of the characteristics of water. The HPSEC method used, equipped with UV and ELSD was able to identify the molecular weight range of NOM present in source water as it confirmed that the Eskom Vaal dam contains humic substances as humic acid and fulvic acid and these pose a health concern as they can form disinfectant byproducts in the course of water treatment with chemicals. FTIR characterization was successful as important functional groups were clearly assigned. Lastly, the use of the TOC and DOC values to calculate SUVA was also a good tool to indicate the organic content in water. It is recommended to use larger amounts of water must be processed to obtain useful quantities of the humic and fulvic acid fractions.

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Influence of humus acids on mobility and biological availability of iron, zinc and copper

Agricultural science and practice ◽

10.15407/agrisp2.01.073 ◽

2015 ◽

Vol 2 (1) ◽

pp. 73-78

Author(s):

A. Fateev ◽

D. Semenov ◽

K. Smirnova ◽

A. Shemet

Keyword(s):

Trace Elements ◽

Organic Matter ◽

Soil Organic Matter ◽

Humic Acids ◽

Fulvic Acids ◽

Sand Culture ◽

Humic And Fulvic Acids ◽

Cultivation Medium ◽

Mobile Forms

Soil organic matter is known as an important condition for the mobility of trace elements in soils, their geo- chemical migration and availability to plants. However, various components of soil organic matter have differ- ent effect on these processes due to their signifi cant differences in structure and properties. Aim. To establish the role of humic and fulvic acids in the process of formation of microelement mobility in soils and their accu- mulation in plants. Methods. A model experiment with sand culture was used to investigate the release of trace elements from preparations of humic and fulvic acids and their uptake by oat plants. Results. It was found that among biologically needed elements humic acids are enriched with iron, fulvic acids – with zinc, and copper distribution between these two groups of substances may be characterized as even. These elements have un- equal binding power with components of soil organic matter, as evidenced by their release into the cultivation medium and accumulation in plants. In the composition of fulvic acids zink has the most mobility – up to 95 % of this element is in the form, accessible for plants; the lowest mobility was demonstrated by copper in the composition with humic acids, for which no signifi cant changes in the concentration of mobile forms in the substrate and in the introduction to the test culture were registered. Despite signifi cantly higher iron content in humic acids, the application of fulvic acids in the cultivation medium provides a greater increase in the con- centration of mobile forms of this element. Conclusions. The results confi rm the important role of organic sub- stances of fulvic nature in the formation of zinc and iron mobility in the soil and their accumulation in plants.

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Uranium(VI) complexation with citric, humic and fulvic acids

Radiochimica Acta ◽

10.1524/ract.2000.88.6.345 ◽

2000 ◽

Vol 88 (6) ◽

Cited By ~ 119

Author(s):

J.J. Lenhart ◽

S.E. Cabaniss ◽

P. MacCarthy ◽

Bruce D. Honeyman

Keyword(s):

Humic Acid ◽

Fulvic Acid ◽

Ph Dependence ◽

Fulvic Acids ◽

Humic And Fulvic Acids ◽

Binding Model ◽

High Affinity ◽

Ph Values ◽

Mononuclear Complexes ◽

Large Size

The binding of uranium(VI) by Suwannee River humic and fulvic acids was studied at pH values of 4.0 and 5.0 in 0.10 M NaClOBoth humic and fulvic acids were demonstrated to strongly bind U(VI), with humic acid forming slightly stronger complexes and exhibiting greater pH dependence. Analyses of the data for the humic and fulvic acid systems using the Schubert´s equation previously applied to the citrate system result in an apparent nonintegral number of ligands binding the uranyl ion. Schubert´s method is only appropriate for interpreting mononuclear complexes with integral moles of binding ligands. Thus, a more elaborate binding model was required and the data were interpreted assuming either: (1) a mixture of 1:1 and 1:2 uranyl-ligand complexes or (2) a limited number of high affinity sites forming a 1:1 complex. While both of these modeling approaches are shown to provide excellent fits to the data, the second is deemed more appropriate given the large size of humic and fulvic acid molecules as well as previous results obtained with other metal cations, such as Cu(II).

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Isolation of bioactive substance from pure mumie

Mongolian Journal of Agricultural Sciences ◽

10.5564/mjas.v11i2.213 ◽

2014 ◽

Vol 11 (2) ◽

pp. 33-35

Author(s):

T Enkh-Oyun ◽

Ts Tsatsralt ◽

J Bayarmaa

Keyword(s):

Biological Activity ◽

Humic Acid ◽

Fulvic Acid ◽

Fulvic Acids ◽

Medical Applications ◽

Asian Countries ◽

Humic And Fulvic Acids ◽

Highly Active ◽

Agricultural Sciences ◽

Bioactive Substance

Mumie contains humid and fulvic acids. For this study, pure mumie was fractionated into fulvic acid (FA) and humic acid (HA) fractions. Humic and Fulvic Acids are highly active bioregulators, and also they contain both macro- and micro-nutrients needed by human and animal bodies. Mumie is stone like material found in Mongolia at higher altitude, which has various medical applications in Mongolia and other Asian countries [2,3]. In the present study, the biological activity of mumie and its fractions was measured. Fractions were prepared according to the protocol described by I. A. Scheretkin and A.I. Khlebnikov. Both humic and fulvic acids can be obtained with the yields of 5.3% and 18.6 % respectively DOI: http://dx.doi.org/10.5564/mjas.v11i2.213 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.33-35

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A Comprehensive Biological and Physico-Chemical Characterization of Humic and Fulvic Acids Nanoparticles as a Perspective Drug

10.20944/preprints202106.0245.v1 ◽

2021 ◽

Author(s):

Elena V. Valer'evna Uspenskaya ◽

Tatiana Vadimovna Pleteneva ◽

Tatiana Vladimirovna Grebennikova ◽

Ilaha Vagifovna Kazimova ◽

Irina Timofeevna Fedyakina ◽

...

Keyword(s):

Humic Acids ◽

Cell Model ◽

Fulvic Acids ◽

Test Method ◽

Biological Study ◽

Fluorescence Signal ◽

Dry Matter Content ◽

Humic And Fulvic Acids ◽

X Ray ◽

Physico Chemical

This work presents the results of a comprehensive physico-chemical and biological study of hu-mic substances samples – an extract of humic and fulvic acids. The performed loss on drying test showed a 22 times different dry matter content between EHS and FA. The morphology and dis-tribution of particles in the dry residue of the samples assessed using the methods of optical and digital microscopy demonstrated differences in the qualitative features of the microstructures of their surfaces and granulometries. Shimadzu X-ray fluorescence spectrometry revealed Si (8.1 and 1.7%), P (33.5 and 2.7%), S (4.3 and 59.5%), K (1.35 and 2.5%), Ca (10.9 and 3.2%), Mn (0.27 and 0.06%), Fe (11 and 0.05%), Cu (0.16 and 0.45%), Zn (0.06 and 0.02%) in the dry residues of the EHS and FA samples, respectively. A high intensity of the X-ray fluorescence signal for Fe atoms in the EHS sample was demonstrated. The FT-IR spectra for EHS and FA are characterized by simi-lar vibration frequencies that are characteristic of the chromone derivatives (1-benzopyran-4-one). The UV absorption spectrum is characterized by max = 281 nm for FA. The EHS solution showed a fluorescence maximum at em = 560 nm at ex = 280 nm. Using the DLS method, nanoparticles of 1 nm and 200 nm were detected in EHS and FA diluted solutions, which are likely to condition the biochemical and physical properties of humic acids. Using the Spiro-tox-test method, the absence of the toxic effect of humic acids on the cell model of ciliates Sp. am-bigua was established. When the cell model was incubated in a solution of a toxicant of the fluo-roquinolone group, a decrease in toxicity was demonstrated when diluted with the EHS solu-tion. The results of the study of the antiviral activity of EHS and FA showed that the study ob-jects in the culture of Vero-E6 cells, in doses non-toxic to cells, suppress the reproduction of the SARS-CoV-2 virus both in the study of the virucidal effect and in the study of the antiviral activ-ity according to the therapeutic and prophylactic model scheme of injection. The results obtained suggest that standardized drugs based on humic acids may open up new perspectives in their biomedical application as antiviral drugs.

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PERMANGANATE OXIDATION OF HUMIC ACIDS, FULVIC ACIDS, AND HUMINS EXTRACTED FROM Ah HORIZONS OF A BLACK CHERNOZEM, A BLACK SOLOD, AND A BLACK SOLONETZ SOIL

Canadian Journal of Soil Science ◽

10.4141/cjss72-004 ◽

1972 ◽

Vol 52 (1) ◽

pp. 43-51 ◽

Cited By ~ 21

Author(s):

S. U. KHAN ◽

M. SCHNITZER

Keyword(s):

Fulvic Acid ◽

Carboxylic Acids ◽

Humic Acids ◽

Fulvic Acids ◽

Oxidation Products ◽

Humic And Fulvic Acids ◽

Chemical Structures ◽

Aliphatic Carboxylic Acids ◽

Different Soils ◽

Solonetz Soil

Humic acid, fulvic acid, and humin fractions extracted from a Black Chernozem, a Black Solod, and a Black Solonetz soil were methylated with diazomethane and oxidized with permanganate solution. The oxidation products were extracted into ethyl acetate, separated by preparative gas chromatography, and identified by comparing their mass and micro-IR spectra with those of authentic specimens. Total yields of oxidation products followed the order humic acids > humins > fulvic acids. The products resulting from the oxidation of humic and fulvic acids averaged 63% benzenecarboxylic, 32% phenolic, and 5% aliphatic carboxylic acids. The oxidation products from humins averaged 76% benzenecarboxylic but only 20% phenolic and 4% aliphatic carboxylic acids, indicating some differences in the chemical structure of humins from those of humic and fulvic acids. The most prominent compounds produced by the oxidation of the humic acids were hydroxy benzenepentacarboxylic and benzenetetracarboxylic acids. In general, differences in the distribution of the major oxidation products between the three major fractions were greater than those within individual humic fractions. Thus, the chemical structures of humic acids extracted from three different soils appeared to be more similar to each other than to those of fulvic acid and humin fractions from the same soil. The same was true for fulvic acids and humins extracted from the different soils. Between 79 and 95% of the oxidation products were identified. The oxidation products may have originated from (a) condensed lignin structures, (b) complex structures of microbiological origin, and/or (c) polymeric structures consisting of benzenecarboxylic and phenolic acids held together by hydrogen-bonding.

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Variation of Δ14C and δ13C Values of Dissolved Humic and Fulvic Acids in the Tokachi River System in Northern Japan

Radiocarbon ◽

10.1017/s0033822200058148 ◽

2013 ◽

Vol 55 (2) ◽

pp. 1007-1016

Author(s):

Seiya Nagao ◽

Takafumi Aramaki ◽

Nobuhide Fujitake ◽

Hiroki Kodama ◽

Takayuki Tanaka ◽

...

Keyword(s):

Humic Acids ◽

Nmr Spectra ◽

River System ◽

Fulvic Acids ◽

Residence Times ◽

Humic And Fulvic Acids ◽

Transport Pathways ◽

River Waters ◽

C Nmr ◽

Northern Japan

Characteristics of dissolved humic and fulvic acids in river waters were studied during 2003–2005 at 4 sites located in the headwaters and in the upper and lower Tokachi River, including a lowland tributary site. Fulvic acids from the headwaters to downstream areas have similar elemental composition and 13C-NMR spectra. Humic acids have similar characteristics in the Tokachi River system. In contrast, δ13C and Δ14C values exhibit a decreasing trend from the upper to the lower and tributary sites, although the headwater site has heavier δ13C and lower Δ14C values than the upper site. Fulvic acids had similar δ13C values from the upper to lower sites, but 123′ higher in Δ14C than those of humic acids on average. The δ13C and Δ14C values exhibited differences in downward variation for humic and fulvic acids. In the Tokachi River system, these results suggest that differences in transport pathways and residence times of humic and fulvic acids reflect differences in the δ13C and Δ14C values in a single river basin.

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Transformations of phenolic compounds in meadow soils

Scientific Reports ◽

10.1038/s41598-020-76316-7 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Anna Ziolkowska ◽

Bozena Debska ◽

Magdalena Banach-Szott

Keyword(s):

Organic Matter ◽

Chemical Composition ◽

Phenolic Compounds ◽

Humic Substances ◽

Humic Acids ◽

Plant Material ◽

Fulvic Acids ◽

Humic And Fulvic Acids

Abstract The aim of the research has been to determine the role of phenolic compounds in the processes of transformations of organic matter in meadow soils, leading to the formation of humic substances. The research has been performed based on the plant material and soil sampled from Europe’s unique complex of permanent grasslands irrigated continuously for 150 years applying the slope-and-flooding system, the Czerskie Meadows. Phenolic compounds were isolated from the plant material samples (hay, sward and roots) and soils (horizon A, AE and Bsv) and from the fraction of humic and fulvic acids. It was found that the contents of phenolic compounds decrease in the following order: hay > sward > roots > A horizon soil > AE horizon soil > Bsv horizon soil > A horizon fulvic acids > AE horizon fulvic acids > Bsv horizon fulvic acids > A horizon fulvic acids > AE horizon fulvic acids > Bsv horizon fulvic acids. A significantly higher share of cinnamyl than vanillyl and syringyl compounds in the extracts of fulvic acids and slightly higher in the hydrolysates of humic acids confirms the effect of the chemical composition of the plant material undergoing decomposition on the properties of the emerging humic substances.

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