scholarly journals Peningkatan Populasi, Pertumbuhan dan Serapan Nitrogen Tanaman Kedelai dengan Pemberian Azotobacter Penghasil Eksopolisakarida

2017 ◽  
Vol 45 (1) ◽  
pp. 30-35 ◽  
Author(s):  
Reginawanti Hindersah ◽  
Neni Rostini ◽  
Arief Harsono ◽  
Dan Nuryani

Nitrogen-fixing Azotobacter is widely used as biofertilizer in sustainable agriculture. The bacteria produce exopolysaccharide which might have a significant role in enhancing soybean nitrogen uptake and growth. The objective of this research was to obtain growth media of Exopolysaccharide–producing Azotobacter; and increase shoot and root growth as well as nitrogen uptake of soybean var. Anjasmoro at early vegetative phase following inoculation of Azotobacter chroococcum liquid. Research consist of two phase, 1) determination of organic-based media for A. chroococcum liquid inoculant production, and 2) pot experiment for application of liquid inoculant on soybean.  The first experiment was performed  in a series of  batch fermenter consisted of several organic media for 72 hours.  The second experiment was set in completely randomized design consisted of three density of liquid inoculant. The results verified that the best media which induced exopolysachharide production of A. chroococcum was 1% molase enriched with 0.1% NH4Cl. Liquid inoculant clearly enhanced population of Azotobacter in soybean rhizosphere, plant height, roots dry weight and N uptake of 21 day old soybean. This research implied that A. chroococcum might be used as biofertilizer at early growth of soybean. Keywords: Azotobacter chroococcum, biofertilizer, liquid inoculat

2021 ◽  
Vol 2 ◽  
pp. 131-138
Author(s):  
Nandung Erlanda ◽  
Feira B. Arief ◽  
Ismahan Umran ◽  
Sutarman Gafur ◽  
Denah Suswati

Azotobacter nitrogen-fixing bacteria and urea fertilizer to reduce excessive use of chemical fertilizers. These bacteria can play a role in increasing nitrogen uptake and further growth of soybean plants. This study aimed to obtain growth media for Azotobacter and increase plant and root growth and nitrogen uptake of Biosoy soybean varieties. The research was conducted to help the uptake of n in the soil and reduce the excessive use of chemical fertilizers. The first experiment was carried out by growing bacteria on the media for 120 hours. The second experiment was designed in a completely randomized design that tested two treatments with additional use of urea fertilizer and Azotobacter and no Azotobacter. The results showed that there was no effect of giving Azotobacter from aloe vera gardens, and urea fertilizer showed a significant effect on the observed variables of soil pH in soybean plants on peat soil. In contrast, the variables observed were population, plant height, N content, N-total, plant dry weight, dry weight of plant roots, and N span of soybean plants had no significant effect.


1970 ◽  
Vol 27 (2) ◽  
pp. 61-64 ◽  
Author(s):  
ARM Solaiman ◽  
MS Talukder ◽  
MG Rabbani

Four strains of Rhizobium were isolated from chickpea (Cicer arietinum) to characterize and observe the effectiveness on host legumes. In a laboratory study, Rhizobium strains showed standard pattern of reactions in respect of growth rate, colony characteristics and acid/alkali production on different growth media. The effect of Rhizobium inoculation on nodulation, growth and nitrogen fixation of chickpea was assessed by pot experiment on clay loam soil. Inoculation treatments comprised of Rhizobium strains CR1, CR2, CR3 and CR4. Rhizobium inoculation had significant positive effects on nodulation, growth and nitrogen fixation of the crop. Among the Rhizobium strains CR2 and CR3 performed better than CR1 and CR4 at 50% flowering stage of the crop. There were high positive correlations among the number and dry weight of nodules, N content and N uptake by shoot of chickpea. Keywords : Rhizobium; Nodulation; Growth; Nitrogen fixation; Chickpea DOI: http://dx.doi.org/10.3329/bjm.v27i2.9174 BJM 2010; 27(2): 61-64


Agrologia ◽  
2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Reginawanti Hindersah ◽  
H Yulina ◽  
Ane Nurbaity

Azotobacter chroococcum based biofertilizers have been widely used in the production of environmentally agriculture. For commercial scale, cheap growth media for Azotobcter inoculant  will affect the quality and price of liquid biofertilizer. Growth medium should support cell proliferation as well as its  nitrogen fixation and phytohormone production.  The objective of this study was to determine whether  liquid organic fertilizer (LOF) containing low N can be used as a medium for scaling up  A. chroococcum inoculant without lowering the survival of bacteria and its activity in N2 fixation and phytohormones production. LOF generally contains a complete nutrient although in a small quantities. Experiment was set up in completely randomized design which tested two types of commercial LOF. The results showed that both of  LOF were only able to support cell growth up to 72 hours and at 96 hours cell viability began to decline compared to the control medium,  free-N Ashby. At 72 hour total N and cytokinin  concentration in LOF was lower than those in Ashby media. This study revealed  that LOF  could be used as A. chroococcum growth media, but without optimization of its composition, cell density and cytokinin content would be lower than those of inoculant  in Ashby Media.


2015 ◽  
Vol 2 (1) ◽  
pp. 54-65
Author(s):  
Nurul Hidayati ◽  
Asro Laelani Indrayanti

The purpose of this research is gaining influence boiler ash on growth and yield of tomatoes, (4) obtain optimal dosing of boiler ash is curuently applied to some media. This research was conducted at Jl. Manjuhan Palangkaraya. The time of this study will last for 4 months This study uses a completely randomized design factorial 2 factors. The first factor is the dose Abu boiler with a 5 stage treotment (without ash, 5 ton.ha-1, l0 ton ha-1, 15 ton.ha-1 and 20 ton.ha-1 . The second factor is the type of media with 3 levels of treatment (Soil sand, soil Podsolid and Peat. The treatment combinotion obtained as-mony as 15 combinations of treatment, with a repeat 3 times. The survey results reveoled that (1) the interaction of growth media and boiler ash dose showed significantly different results against 2,4 dan 6-wieks after planting variables plant height, amount of leaves on the age, 2, 4 and 6 weeks after, plantig, stem diameter at 4 weeks afier planting, and shoot dry weight lastest research. Treatment combination boiler ash 15 ton / ha and peat media (treatment b4t3) provide significant variahles plant height, leaf amount, diameter of stock, and shoot dry weight.


2021 ◽  
Vol 914 (1) ◽  
pp. 012055
Author(s):  
T M Hasnah ◽  
E Windyarini ◽  
B Leksono ◽  
K Riyantika ◽  
B H Purwanto ◽  
...  

Abstract The solid waste (dregs seed) of nyamplung (Calophyllum inophyllum) industry from seed pressing processes could be more than 50% of the dry seed weight. Nyamplung oil industry in Bantul (Yogyakarta) has been produced nyamplung oil 4-5 ton/month that produced dregs seed around 50-60%/ton nyamplung dry seeds. The accumulated waste has been piled up and immediately important to utilize it. This study aimed to determine (i) the effect of bioactivators with ameliorant material application and the interactions among treatments on nyamplung dregs compost quality, and (ii) the growth response, seedling quality index and nitrogen uptake on nyamplung seedlings with nyamplung dregs compost application. The study was arranged in Completely Randomized Design (CRD) factorial. Two bioactivators Prouponic Gb#1 and cattle rumen bio starter were used in the study. The bioactivators were combined by ameliorants (no ameliorant, dolomite, and rice husk ash) with 3 replicates for each treatment. Analyses of soil chemical properties were carried out on nyamplung dregs before and after the treatments applied including: pH, DHL, N Total, C/N ratio, P Total, K Total. Those characters were compared to SNI No.19-7030-2004 on compost quality standards. DHL, C/N ratio, the total N, P, and K met the criteria on SNI. Seedling growth characters (height, diameter, number of leaves) and seedling quality index varied between nyamplung dregs compost treatments and control up to 16 weeks observation. Nyamplung dregs compost application showed the highest N uptake on nyamplung seedlings compared to the controls. Nyamplung seedling quality index was highly influenced by the uptake of N nutrients in the shoots and the roots than by height character and diameter.


2014 ◽  
Vol 2 (3) ◽  
pp. 49 ◽  
Author(s):  
Anita Dewi Agustin ◽  
Melya Riniarti ◽  
Duryat .

To support the cultivation of yellow cempaka (Michelia champaca) intensively, high quality seedlings was needed. One of the factors that affect the growth and quality of seedling was growth media. The objectives of the research were to (1) determine the effect of saw dust and rice husk as growth media than the top soil for yellow cempaka seedlings; (2) obtained the best composite media between top soil with saw dust and rice husk as media for growth media for yellow cempaka seedlings. The research was conducted in greenhouse, started from January to April 2013. The experiment used Completely Randomized Design (CRD) with 6 treatments and 3 replicates, every experiment unit consisted of 6 yellow cempaka seedlings so the total number amounted to 108 seedlings. The treatment were number of medias (1) top soil; (2) saw dust; (3) rice husk; (4) top soil + saw dust 1:1; (5) top soil + rice husk 1:1; (6) top soil + saw dust + rice husk 1:1:1.  Observed variables include high growth, diameter, number of leaves, root length, top root ratio, total dry weight and seedling quality index. The results showed that growth media of rice husk gave a good growth of yellow cempaka same as media top soil.  Composite growth media that had a better growth of yellow cempaka seedling was composite growth media top soil + rice husk 1:1.


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 822-826 ◽  
Author(s):  
Qingzhang Xu ◽  
Bingru Huang

Roots play important roles in plant responses to environmental changes. The objective of this study was to investigate seasonal changes and cultivar variation in root growth, respiratory activity, nitrogen uptake, and carbon allocation in relation to turf performance for two cultivars of creeping bentgrass [Agrostis stolonifera L. var. palustris (Huds.)] under field conditions. Two cultivars, `Penncross' and `L-93', were managed under USGA-specification putting green conditions, with daily irrigation and mowing at a 3-mm height from May to November in 1999 and 2000. Turf quality of both cultivars declined from the highest rating of 7 to 9 in May to 4 to 5 in August and September, and recovered to above 7 in October and November in both years. This corresponded to seasonal changes in root dry weight, dehydrogenase activity, nitrate reductase (NR) activity, carbon allocation to roots, and 15N uptake. Compared to Penncross, L-93 generally maintained better turf quality, as well as higher root dry weight, 15N uptake, NR activity, and carbon allocation during summer months. Previous studies often emphasize the important of a large, extensive root system. The results in the present study demonstrated that root metabolic activities followed the same seasonal pattern and cultivars variation as turf performance, and suggested that decline in root metabolic activities could be contributed to summer decline in turf quality for creeping bentgrass.


2021 ◽  
Vol 911 (1) ◽  
pp. 012043
Author(s):  
M. Soedarjo ◽  
P. Sasmita

Abstract Porang (Amorphophallus muelleri Blume) is usually cultivated on the field under the tree as natural shading. Porang can also be grown on a pot containing limited amount of growth media. The present research work was carried to evaluate the minimum amount of growth media required for optimum growth and corm yield. The study used bulbils (10 g/bulbil and 20 g/bulbil) and corm (+ 15 g/corm) sown on growth media of 2.5 kg, 5.0 kg and 7.5 kg/pot as treatments. Each treatment was arranged in completely randomized design and was replicated 3 times. Plant height, plant diameter, shoot dry weight, diameter and thickness of corm and fresh weight of corm were measured to evaluate the influence of the treatments on growth and corm yield of porang. The plant growth of porang measured as plant height, plant diameter, shoot dry weight was improved when the planting materials were planted on bigger size of growth media. Bigger size of bulbil caused better growth of porang on each growth medium. The effect of plant materials and the size of growth media on porang growth was in parallel to its effect on corm yield measured as corm diameter, corm thickness and fresh weight of corm. The result of the present study suggests to use bigger bulbil and bigger size of growth media when planted on polyethylene bag. Corm as planting material was better than bulbil because less size of corm produced similar plant growth and corm yield compared to bigger size of bulbil. Therefore, the use of corm as planting material is more favorable to bulbil.


2019 ◽  
Vol 7 (3) ◽  
pp. 332
Author(s):  
Devi Aprillia ◽  
Melya Riniarti ◽  
Afif Bintoro

Ex-limestone mining quarry has poor soil fertility, both biological, chemical, and physical condition due to the mining process. Marginal land conditions such as ex-limestone mining quarry require high survival seedlings. Application of ectomycorrhizal fungi is an alternative that can be done to improve the success of restoration. Acacia mangium is one of the plants that could be colonized by many types of mycorrhizal such as ectomycorrhiza. This research aimed to determine the growth of A. mangium seedlings in ex-limestone mining growth media and the effect of ectomycorrhiza application on the growth of A. mangium. The research used a completely randomized design with six treatments and five replications. Treatment consisted of 100% soil with mycorrhiza, 100% soil without mycorrhiza, 50% soil + 50% limestone tailings + mycorrhiza, 50% soil + 50% limestones tailings without mycorrhiza, 100% tailing with mycorrhiza and 100% tailing without mycorrhiza. The seedlings used in the study germinated from seeds taken from Lampung Timur Regency. The seeds germinated in sand for one month, then used as the research material. The measurement taken were  height, diameter, number of leaves, leaf area, measured were root length, canopy dry weight, and total dry weight. The colonization of ectomycorrhiza is measured by counting the percentage of colonization. The symbiosis with rhizobium measured by number of root nodules. Data were tested for variance analysis, followed by Least Significance Different Test at significant level of 1% and 5%. The results showed that the percentage of A. mangium life was high, reaching 100%. All growth parameters showed that plant colonized by mycorrhiza has remarkably better values compared to non-mycorrhiza plants.Keywords: limestone, ectomycoriza, mangium, phytoremediation


2018 ◽  
Vol 8 (3) ◽  
pp. 154-159
Author(s):  
Hariadi Saputra ◽  
Yadi Setiadi ◽  
Iwan Hilwan

Nursery is a process in producing good quality seedlings. Seedling quality is determined by physical-physiological quality including woody stem, health, height, diameter, number of leaves, leaf color and compactness of media. The quality of angsana seedlings in this study was observed using some growth media, lateral root cutting, and cow’s urine addition with various concentration and liquid organic fertilizer for 13 weeks. The study was conducted in a completely randomized design (CRD) in split plot with 3 growth mediums, 10 combinations of root-cutting and fertilizing treatment using 9 seedlings per treatment. Parameters observed during this study were seedling height (cm), seedling diameter (mm), total dry weight (g), shoot - root ratio (RPA) and compactness of roots. The results showed the media contain mixture of soil and manure (M2) with root-cutting treatment, addition of 20% cow urine and 5% EM4 (A2) was the best treatment for angsana seedlings with average value of 46.25 cm plant height, 6.09 mm diameter, 7.77 g total dry weight, shoot root ratio 1.89 and compactness of roots including the compact category.Key word : cow’s urine, lateral roots cutting, Pterocarpus indicus Willd., seedling quality.


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