scholarly journals HISTOLOGICAL STRUCTURE OF THE KINTAMANI DOG ON THE SEXUAL MATURITY PERIOD

Author(s):  
Ni Luh Eka Setiasih ◽  
Putu Suastika ◽  
Luh Gede Sri Surya Heryani ◽  
Ni Nyoman Werdi Susari

The objective of this study was to determine the histological structure of uterus of the kintamani dogs on the sexual maturity period. In this study using five samples of uterine organs of the kintamani dog. The tissue were taken from corpus of the uterine. The histological structure was observed using hematoxylin-eosin (HE). Identification of the histological structure of uterus observed with a microscope at 100x and 400x magnification. The results showed the endometrial lining   consisted a single layer  of the columnar epithelium and lamina propria with tubular glands, myometrium consisted of smooth muscle and perimetrium with connective tissue, nerves and blood vessels

2020 ◽  
Vol 151 ◽  
pp. 01052
Author(s):  
Dian Masyitha ◽  
Lena Maulidar ◽  
Zainuddin Zainuddin ◽  
Muhammad N. Salim ◽  
Dwinna Aliza ◽  
...  

This research aimed to study the histology of the digestive system of the watersnake (Enhydris enhydris). The digestive system taken was the esophagus, stomach, frontal small intestine and the back of the large intestine from three watersnakes. The samples were then made into histological preparations with hematoxylin-eosin (HE) staining and observed exploratively. The results showed that the digestive system of the watersnake was composed of layers of tissue, namely the mucosa, tunica submucosa, tunica muscularis, and serous tunica. Mucosal mucosa consisted of the lamina epithelium, lamina propria, and mucous lamina muscularis. The submucosal tunica consisted of connective tissue with blood vessels, lymph, and nerves. The muscular tunica was composed of circular muscles and elongated muscles. The serous tunica consisted of a thin layer of connective tissue that was covered by a thin layer of the mesothelium (mesothelium). The histological structure of the snake digestive system is not much different from the reptile digestive system. However, in the small intestine of the snake, Brunner, and liberkhun glands are not found. Lamina epithelial in the small intestine of the snake is composed of the layered cylindrical epithelium.


2017 ◽  
Vol 19 (78) ◽  
pp. 26-29
Author(s):  
N.V. Dyshlyuk

The features of the structure of the esophagus and its lymphoid tissue of the house sparrow (Passer domesticus) have been studied. It is shown that its wall is formed by well expressed mucous, muscular and least developed adventitial (serous in the caudal part) shells. The mucosa forms 7–9 longitudinal folds of leaf-shaped and finger-shaped forms directed into the lumen of the esophagus. It consists of epithelium, lamina propria, lamina muscularis and submucosa. The epithelial layer is represented by a multi-layered flat, weakly keratinizing epithelium and is better developed in the cranial part of this organ. Near the proventriculus, it passes into a single-layered cylindrical. In its lamina propria of the mucosa there are bags of numerous large esophageal glands in which mucus is recorded. Their excretory ducts open onto the surface of the mucosal epithelium. The lamina muscularis is intermittent in places, formed by a smooth muscle tissue. The submucosa is weakly expressed and its own plate is formed by a loose fibrous connective tissue with blood vessels. Between the esophagus glands, their excretory ducts and in the submucosa of the tunica mucosa on the border with the tunica muscular are separate clusters of diffuse lymphoid tissue, which are represented by cells of the lymphoid series without noticeable rarefaction and densities. The basis of the diffuse lymphoid tissue is the reticular tissue, the fibers of which, are densely disposed and do not have a definite orientation and form a small grid. In the area of transition of the esophagus to the proventriculus, the esophageal tonsil, which is inherent in many species of birds, is not expressed. In this area in the mucous membrane, only local accumulations of diffuse lymphoid tissue are recorded. There are no prenodules and lymphoid nodules. The tunica muscular of the esophagus is formed by a smooth muscle tissue, which forms the inner–circular and outer – longitudinal layers. Between them, layers of loose fibrous connective tissue and blood vessels are identified. Tunica adventitia (serosa) are formed by a loose fibrous connective tissue, and serosa is also covered by mesothelium.


1955 ◽  
Vol 6 (2) ◽  
pp. 139 ◽  
Author(s):  
R Endean

The test of this species is particularly bulky and consists of gelatinous material which encloses the fleshy part of the animal, leaving openings only for the two siphons. It also forms a resilient basal stem which anchors the animal to the substratum. The outer surface of the test is impregnated with sand grains, which form a hard protective layer. Blood-vessels traverse the gelatinous material. Two large vessels enter the test and these branch to form ultimately a ramifying system of small blood-vessels. Effectively there are two blood circulations in the test. The blood-vessels have a lining consisting of a single layer of large columnar cells, the appearance of which is described. The gelatinous test resolves itself under the microscope into a network of fibres of variable thickness, but the larger ones at least are aggregates of smaller fibrils. In some cases the fibres are orientated in definite directions. The amorphous interfibrillar material seems to be mainly aqueous, as the test consists of over 97 per cent. of plasma. Ferrocytes wander about in amoeboid fashion amongst the fibres. These enter the substance of the test by migrating across the walls of the bloodvessels. Histologically there is a marked similarity between the appearance of the test and that of vertebrate connective tissue, as in both cases cells are present in a groundmass enmeshed by fibres which stain with orcein and fuchsin. However, the fibres of the test are composed of a very insoluble polysaccliaride, Which although resembling plant cellulose in certain respects will not dissolve in special cellulose solvents nor stain with cellulose stains. A mucopolysaccharide which stains metachromatically with toluidine blue is associated with the fibrillar material, and possibly serves to cement the fibres together since these became disarranged on treatment with hyaluronidase, which removes the mucopolysaccharide. The importance of the test in the general economy of the animal is discussed.


2017 ◽  
Vol 35 (1) ◽  
pp. 111
Author(s):  
Mahfud Mahfud ◽  
Adi Winarto ◽  
Chairun Nisa

Varanus salvator bivittatus has a pair of hemipenes, which is macroscopic anatomically like the copulatory organ in other amniotes, cylindrical-shaped (truncus) with quite flexible, and it located on the base of the caudal tailof the cloaca. However, information about the microscopic anatomy of hemipenes of this animal is scientifically unpublicized. Therefore, the aim of this research is to study the microscopic anatomy of hemipenes of male Varanussalvator bivittatus. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion then observed visceral site and morphometric. Histomorphological evaluationwas obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE) and Masson’s Trichrome (MT). The results showed that truncus of hemipenes was lined by stratified squamousepithelium and supported with thick of dense connective tissue and contain cavernous body and blood vessels are found, the muscles not found. The presence of connective tissue that supported in the down part sometimesmake hemipenes are rigid while prurient condition. In the caudal of truncus hemipenes there is retractor muscle of hemipenes which arranged by striated muscles. Hemipenes is flexible because contain with much of blood vesselthat found in truncus hemipenes.


2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Aliman Irfandi ◽  
Cut Dahlia Iskandar ◽  
Zainuddin Zainuddin ◽  
Dian Masyitha ◽  
Fitriani Fitriani ◽  
...  

Domestical catfish is grouped into omnivora, although the fish is tended into carnivora.  The aim of the present study was to investigate histology of alimentary tract of domestical catfish (Clarias batrachus). In order to determine alimentary tract of domestical catfish, oesophagus, gastric and intestines is investigated by Haematoxilin Eosin. Histology exploration was performed each layer of alimentary tract’s wall consist of lamina epithelia, propria, and muscularis mucosa. Collagen connective tissue was developed tunica submucosa. The muscle of tunica muscularis was performed circular and elongated. Dense connective tissue and blood vessels was located in the tunica serosa. Histology exploration revealed has stratified squamous epithelium, mucosit cells, eosinophilic club cells on the lamina epithelia, and taste buds on the lamina propria in oesophagus. Gastric was establised simple prismatic cells, and glands of gastric on the lamina propria. Furthermore intestine was showed villi and goblet cells. There were not showed Brunner and Liberkhun gland. In conclusion, histology of alimentary tract was revealed from tunica mucosa, submucosa, and serosa.


2000 ◽  
Vol 24 (2) ◽  
pp. 169-178
Author(s):  
N. S. Al-Samarrae*, ◽  
KH. A. Al-Jobori ◽  
KH. A. Al-Hathry ◽  
Al-Shamary. B. F.

The epididymal region of the common quail was located on the dorso-medial aspect of the testis, extending from cranial pole to the caudal pole of the testis and connected the urodeum via the vas deferens and the receptaculum. The ductus epididymidis in common quail showed a series of coiled ductules which were closely bounded to the testis by connective tissue. The lining epithelium of these ductules were ciliated columnar epithelium, but non ciliated columnar epithelium were also noticed.


2020 ◽  
Vol 151 ◽  
pp. 01051
Author(s):  
Zainuddin Zainuddin ◽  
Nurul Fadhilah ◽  
Dian Masyitha ◽  
Muhammad N. Salim ◽  
Erdiansyah Rahmi ◽  
...  

Rainbow watersnake is a snake that can be found in ponds, has a relatively medium body, a maximum length of 80 cm, generally between 50-60 cm, small-headed, a rather large belly, and short-tailed. The aim of this study is to determine the structure of the histology of the lungs of watersnake (Enhydris enhydris). This study used a sample of 3 watersnakes (Enhydris enhydris) with a body length of ± 50 cm. This study conducted a micro technical method with hematoxylin-eosin (HE) staining. Data obtained from the results of this study were analyzed descriptively and presented in the form of images. Macroscopic observations of the warp in snakes are long, most of the trachea attaches to the warp, and there are water sacs or swimming pouches at the tip of the warp, as well as the histological features of the snakes' warp, alveolar ducts, alveolar sac and alveoli, the histological structure of the lungs in watersnakes (Enhydris enhydris) consist of type 1 alveoli epithelial cells, type 2 alveoli cells, muscle bundles, connective tissue, capillary vessels, and macrophage cells. Based on the results of the study, it can be concluded that the histological structure of the watersnake (Enhydris enhydris) lung is different from the histological structure of the reptile function in general. In the lungs of watersnakes (Enhydris enhydris) only consists of alveolar ducts, alveolar sacs, alveoli, and the presence of water sacs at the ends of the lungs.


2021 ◽  
Vol 14 (2) ◽  
pp. 5-12
Author(s):  
Yahia Y.k. ◽  
Khalid K.K.

Current work was conducted to investigate the histological architecture of the uterine tube and uterus during the period of late pregnancy in rabbits. Twelve adult local breed rabbits were used. The samples from different parts of the uterine tube were prepared for histological study after staining with H&E, Masson’s trichrome and combine Alcian blue (pH2.5)-PAS. The pre ampulla was a narrow tube and its tunica mucosa had slightly long simple mucosal folds lined by pseudostratified columnar epithelium, the ampulla had a wide lumen and its tunica mucosa displayed several highly tall branched mucosal folds with few short simple folds while the isthmus was the narrowest region and its tunica mucosa displayed few of tall and short simple mucosal folds. The mucosa of three parts of the uterine tube was lined by pseudostratified columnar epithelium which is composed of three types of cells: mucous secretory cells, non-secretory cells and basal cells, the mucous secretory cells were the predominant type and revealed secretory activities. The lamina propria-submucosa of the uterine tube was composed of cellular connective tissue and tunica muscularis. The uterus had a very thick wall with well-recognized endometrium and myometrium, the endometrium was composed of many-branched and simple endometrial folds that were covered by multinucleated syncytial cells and simple cuboidal epithelial cells. Lamina propria was composed of loose connective tissue had scattered groups of well growing simple uterine glands that showed secretory activities. The current result showed that the uterus during the late period of pregnancy was differed from those of non-pregnant rabbits, while the uterine tube at late pregnancy has a structure that appeared prepared for the next physiological period.


2012 ◽  
Vol 28 (2) ◽  
pp. 75-79
Author(s):  
K. A. Ferdous ◽  
M. N. H. Parvez ◽  
M. T. Rahman

Structure and postnatal development of vagina in 21 ISA brown chickens was studied at three, 23 and 46 weeks of age. At 3 weeks of age the vagina was narrower and thicker then the uterus. The undifferentiated wall of vagina consisted of low primary mucosal folds lined by simple columnar epithelium to a pseudostratified ciliated columnar epithelium and a connective tissue layer underneath. At three weeks of age, scattered smooth muscle fibres were in the subepithelial connective tissue layer. Rapid structural changes occurred at 23 weeks of age in the adult bird, mucosal folds were narrowed and tongue-shaped. Lamina propia was devoid of glands and contained lymphocytes. Tunica muscularis was well developed at 23 and 46 weeks.DOI: http://dx.doi.org/10.3329/bvet.v28i2.10676Bangl. vet. 2011. Vol. 28, No. 2, 75 – 79


Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 2564-2564
Author(s):  
Mario Von Depka ◽  
Hanna Allerkamp ◽  
Mahnaz Ekhlasi-Hundrieser ◽  
Christiane Pfarrer ◽  
Carsten Detering ◽  
...  

Abstract Objectives: Women with von Willebrand Disease (VWD) type 3 may develop miscarriage during pregnancy. There is evidence that besides its role in coagulation von Willebrand Factor (VWF) is also involved in angiogenesis and defects of this pathway could play a role in miscarriage. For this reason, VWF as well as proteins with known functions in angiogenesis were analysed by immunohistochemistry of several tissues in a pig model comprising all VWD type 3 genotypes (VWD, heterozygous carriers, wildtype). Methods: Uterus tissue samples including the endometrium were collected from a total of 6 pigs. Of these, two were affected by VWD type 3, two were heterozygous carriers, and the remaining two were wildtype individuals, which served as controls. Hematoxylin-eosin-staining was implemented for morphological evaluation of the tissues, especially with regard to possible differences in structure and pattern of blood vessels. In a second step immunohistochemical analyses comparing the expression of angiogenic factors including VWF were performed in VWD-animals, genetic carriers and wildtype pigs. Results: Hematoxylin-eosin-staining of histological sections of the porcine uteri comprising the different genotypes revealed differences in the vascular architecture within the lamina propria. Compared to non pregnant wild type pigs where blood vessels in the lamina propria of the endometrium usually are of smaller caliber (mostly categorized as arterioles, venules and capillaries), the lamina propria of VWD type 3 animals contained multiple large arteries and veins (of the type usually found in the uterine wall). The immunohistochemical analysis for VWF showed almost no VWF expression in the pigs affected by VWD. In wildtype and heterozygous pigs, expression was obvious in the endothelium. A narrow band of VWF expression apically on the epithel cells as well as partial expression in glands was seen only in wildtype pigs, but not in heterozygous pigs. For some, but not all further angiogenic factors analysed, expression differences were present among the porcine VWD genotypes. Conclusion: The results of our study confirm effects of VWF on the uterine vascular architecture and on the expression of specific angiogenic factors. Pigs affected by VWD showed almost no expression of VWF and the blood vessels within the uterine lamina propria had a surprisingly high percentage of large arteries and veins indicating a crucial role of vWF for uterine angiogenesis. Expression differences among the genotypes were obvious for specific angiogenic factors. Disclosures Von Depka: Octapharma: Other: Study Investigator, Speakers Bureau.


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