scholarly journals Morfologi Hemipenis Biawak Air (Varanus salvator bivittatus)

2017 ◽  
Vol 35 (1) ◽  
pp. 111
Author(s):  
Mahfud Mahfud ◽  
Adi Winarto ◽  
Chairun Nisa
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Copulatory Organ ◽  
Retractor Muscle ◽  
Striated Muscles ◽  
Section Thickness ◽  
Microscopic Anatomy ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Cavernous Body

Varanus salvator bivittatus has a pair of hemipenes, which is macroscopic anatomically like the copulatory organ in other amniotes, cylindrical-shaped (truncus) with quite flexible, and it located on the base of the caudal tailof the cloaca. However, information about the microscopic anatomy of hemipenes of this animal is scientifically unpublicized. Therefore, the aim of this research is to study the microscopic anatomy of hemipenes of male Varanussalvator bivittatus. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion then observed visceral site and morphometric. Histomorphological evaluationwas obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE) and Masson’s Trichrome (MT). The results showed that truncus of hemipenes was lined by stratified squamousepithelium and supported with thick of dense connective tissue and contain cavernous body and blood vessels are found, the muscles not found. The presence of connective tissue that supported in the down part sometimesmake hemipenes are rigid while prurient condition. In the caudal of truncus hemipenes there is retractor muscle of hemipenes which arranged by striated muscles. Hemipenes is flexible because contain with much of blood vesselthat found in truncus hemipenes.

scholarly journals HISTOLOGICAL STRUCTURE OF THE KINTAMANI DOG ON THE SEXUAL MATURITY PERIOD

2019 ◽  
pp. 39
Author(s):  
Ni Luh Eka Setiasih ◽  
Putu Suastika ◽  
Luh Gede Sri Surya Heryani ◽  
Ni Nyoman Werdi Susari
Keyword(s):  
Smooth Muscle ◽  
Connective Tissue ◽  
Blood Vessels ◽  
Lamina Propria ◽  
Sexual Maturity ◽  
Single Layer ◽  
Columnar Epithelium ◽  
Histological Structure ◽  
Maturity Period ◽  
Hematoxylin Eosin

The objective of this study was to determine the histological structure of uterus of the kintamani dogs on the sexual maturity period. In this study using five samples of uterine organs of the kintamani dog. The tissue were taken from corpus of the uterine. The histological structure was observed using hematoxylin-eosin (HE). Identification of the histological structure of uterus observed with a microscope at 100x and 400x magnification. The results showed the endometrial lining   consisted a single layer  of the columnar epithelium and lamina propria with tubular glands, myometrium consisted of smooth muscle and perimetrium with connective tissue, nerves and blood vessels

scholarly journals Histology of Watersnake (Enhydris Enhydris) Digestive System

2020 ◽  
Vol 151 ◽  
pp. 01052
Author(s):  
Dian Masyitha ◽  
Lena Maulidar ◽  
Zainuddin Zainuddin ◽  
Muhammad N. Salim ◽  
Dwinna Aliza ◽  
...  
Keyword(s):  
Small Intestine ◽  
Connective Tissue ◽  
Thin Layer ◽  
Blood Vessels ◽  
Lamina Propria ◽  
Large Intestine ◽  
Digestive System ◽  
Histological Structure ◽  
Tunica Muscularis ◽  
Hematoxylin Eosin

This research aimed to study the histology of the digestive system of the watersnake (Enhydris enhydris). The digestive system taken was the esophagus, stomach, frontal small intestine and the back of the large intestine from three watersnakes. The samples were then made into histological preparations with hematoxylin-eosin (HE) staining and observed exploratively. The results showed that the digestive system of the watersnake was composed of layers of tissue, namely the mucosa, tunica submucosa, tunica muscularis, and serous tunica. Mucosal mucosa consisted of the lamina epithelium, lamina propria, and mucous lamina muscularis. The submucosal tunica consisted of connective tissue with blood vessels, lymph, and nerves. The muscular tunica was composed of circular muscles and elongated muscles. The serous tunica consisted of a thin layer of connective tissue that was covered by a thin layer of the mesothelium (mesothelium). The histological structure of the snake digestive system is not much different from the reptile digestive system. However, in the small intestine of the snake, Brunner, and liberkhun glands are not found. Lamina epithelial in the small intestine of the snake is composed of the layered cylindrical epithelium.

Studi Histokimia Usus Biawak Air (Varanus salvator bivittatus) (Reptil: Varanidae)

2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ernawati
Keyword(s):  
Tissue Sample ◽  
Histological Section ◽  
Tissue Perfusion ◽  
Intestinal Wall ◽  
Biological Information ◽  
Suitable Habitat ◽  
Monitor Lizard ◽  
Varanus Salvator ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Biological information, behaviour and suitable habitat of water monitor was very less in order to support its maintenance management and breeding efforts. One of important information is the information of digestive tract, particularly the information about the structure of intestine tissue of water monitor. Sample in this research was intestine organ of water monitor. The animal was anesthetized, exanguinated, and fixed in paraformaldehyde 4% by tissue perfusion method. The intestine tissue sample for histological section with paraffin method was cutted with 3-4 μm thick and coloured with hematoxylin eosin (HE). Observation were performed to the structure of intestine histology. The results was analysed descriptively and presented in figures. Monitor lizard intestine consist of small intestine and large intestine. The small intestinal wall was observed similar to jejunum and ileum. The large intestinal wall was composed of transitional ephytelia and connective tissue. However, the ephytelial layer in this tissue was composed of transitional ephytelia that similar to vesica urinaria and there are no villi.

scholarly journals Enamel Matrix Derivative in Diffusion Chamber Implanted Subcutaneously in Rat Induces Formation of Fibrous Connective Tissue Containing Abundant Blood Vessels

In Vivo ◽  
2021 ◽  
Vol 35 (1) ◽  
pp. 313-317
Author(s):  
SATOSHI YOKOSE ◽  
YUKA KATO ◽  
KATSUTOSHI MATSUMOTO ◽  
PERRY R. KLOKKEVOLD ◽  
HENRY H. TAKEI ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Diffusion Chamber ◽  
Enamel Matrix Derivative ◽  
Fibrous Connective Tissue ◽  
Enamel Matrix ◽  
Matrix Derivative

scholarly journals Morphofunctional and immunohistochemical characteristics of the large omentum in ovarian cancer

2020 ◽  
Vol 9 (3) ◽  
pp. 64-71
Author(s):  
N. N. Shevlyuk ◽  
L. V. Khalikova ◽  
A. A. Khalikov
Keyword(s):  
Ovarian Cancer ◽  
Connective Tissue ◽  
Protein Expression ◽  
Blood Vessels ◽  
Lymphoid Tissue ◽  
P53 Protein ◽  
Individual Variability ◽  
The Body ◽  
Great Omentum ◽  
Low Grade

The aim of the study was to establish morphofunctional and immunohistochemical characteristics of large omentum in women with ovarian cancer.Material and methods. The large omenta of 48 women with ovarian cancer (low-grade differentiated seropapillary adenocarcinoma of high-grade malignancy) of II stage (n=20) and III stage (n=28) were studied. Histological sections were stained with overview histological and immunohistochemical methods (to reveal ki67, P53, CD34, CD7, CD4, CD8, CD61 proteins expression). Results. In patients, the size of the large omentum was characterized by high individual variability; in the presence of metastasis, the size of the omentum was reduced. Intensive development of blood vessels in the organ was noted, but in the presence of metastases stasis of blood corpuscles, leucocytic infiltration, and moderate edema of connective tissue were observed in the organ’s vessels. Areas of lymphoid tissue, both small lymphatic follicles and diffusely located lymphoid tissue, were revealed in the omentum. In most follicles, reactive centers were not marked, and the number of follicles was reduced in the presence of metastases in the omentum. The analysis of CD34+ cells distribution showed that they were identified both in the tumor and in the areas of the omentum adjacent to the tumor, which indicates a pronounced angiogenesis. An irregular distribution of CD7+ and CD8+ and CD4+ cells was revealed in the tumor tissues, as well as in the surroundings. Simultaneously with the expression of P53 protein, ki67 protein expression is revealed in the significant number of tumor cells (including endothelial cells of tumor blood vessels). The proportion of ki67+ cells in the tumor cell population was 60.1±3.3%. The presence of a large number of ki67+cells in the presence of P53 protein expression in them indicates the aggressiveness of the tumor, as well as a disturbance of apoptosis regulatory mechanisms in the cells. Ki67 expression was low in the omentum areas unaffected by metastases, and it was revealed in the certain areas of connective tissue in fibroblastic programmed differentiation cells. Conclusion. The results obtained indicate significant plasticity and reactivity of great omentum in the presence of tumor process in the body and confirm the important role of great omentum in protective reactions.

scholarly journals FACTORS INFLUENCING THE INTERMITTENT PASSAGE OF LOCKE'S SOLUTION INTO LIVING SKIN

1941 ◽  
Vol 73 (1) ◽  
pp. 85-108 ◽  
Author(s):  
Philip D. McMaster
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Atmospheric Pressure ◽  
Interstitial Fluid ◽  
Lymphatic Vessels ◽  
Intermittent Flow ◽  
Venous Obstruction ◽  
Factors Influencing ◽  
Interstitial Connective Tissue ◽  
Living Mouse

Minute amounts of Locke's or Tyrode's solution have been brought into contact with the interstitial connective tissue of the skin of the living mouse, at atmospheric pressure, in such a manner that the blood or lymphatic vessels are not entered directly. Under such circumstances these absorbable fluids enter the tissue spontaneously. Entrance is strikingly intermittent, not continuous, and so too when very slight pressures are brought to bear on the fluids (1). Hyperemia of the tissues, with accompanying dilatation of the blood vessels, increases the entrance of fluids at atmospheric pressure but it is still intermittent. By contrast, venous obstruction leads to intermittent backflow into the apparatus, but reflex hyperemia, following release of the obstruction, is attended by an increase of flow into the tissues in spite of the great reactive dilatation of vessels. The inflow is also intermittent. If the skin is deprived of circulation, fluid does not enter it at all at atmospheric pressure, though it moves in regularly and continuously if slight pressure is put upon it. Edema-forming fluids, described in the text, also enter in a continuous manner when forced into the skin of either living or dead animals. So too do serum and sperm oil. The findings indicate that the passage of interstitial fluid into the blood vessels may be intermittent under normal circumstances and its escape from them as well. The observed occurrence of intermittent flow in the blood vessels of several tissues (9, 15–25) will go far to account for the intermittent entrance of fluid into the skin.

On the Pituitary of the Perch (Peroa Fluviatilis)

1942 ◽  
Vol s2-83 (331) ◽  
pp. 299-316
Author(s):  
T. KERR
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Perca Fluviatilis ◽  
Cell Types ◽  
Anterior Lobe ◽  
Intermediate Lobe ◽  
General Description ◽  
Perch Perca Fluviatilis ◽  
Higher Types ◽  
Different Cell Types

1. A general description is given of the pituitary of the perch (Perca fluviatilis L.), and histological details of its various parts. The subdivisions of the glandular component are confluent with each other but distinguished by their different cell types. The nervous lobe makes contact with all three of the subdivisions, but is separated from them by a layer of connective tissue, incomplete in particular areas. 2. The anterior glandular region (anterior lobe) has an anterior chromophil and a posterior chromophobe zone. The middle glandular region (transitional lobe) possesses brightly staining acidophils and basophils as well as chromophobes. The acidophils form a dorsal sheet, deeply indented by processes of the nervous lobe, the basophils lie ventrally and posteriorly, and chromophobes are common towards the extremities of the indentations. The posterior glandular region (intermediate lobe) is elaborately penetrated by nervous lobe processes; the cells are small and consist of amphiphils, dull basophils, and occasional dull acidophils. The possible homologies of these regions to the lobes of higher types are discussed. The nervous lobe is of loose glial tissue with many nuclei and blood vessels and some reticular and collagenous fibres. 3. Strongly acidophil spheres of various sizes and in various numbers occur in the middle glandular region. They originate in ‘sphere cells’ resembling eosinophil leucocytes and after enlarging become free in the tissues of the region. Later they appear to pass into the posterior processes of the nervous lobe to be the larger bodies of the Herring material. Finally these larger elements appear to break down to form a fine granulation, whose further fate could not be followed.

Light and Scanning Electron Microscopy of Human Spinal Ligaments

1985 ◽  
Vol 55 ◽  
Author(s):  
L.-H. Yahia ◽  
G. Drouin ◽  
C.-H. Rivard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Connective Tissue ◽  
Idiopathic Scoliosis ◽  
Blood Vessels ◽  
Structural Changes ◽  
Loose Connective Tissue ◽  
Morphological Studies ◽  
Spinal Ligaments ◽  
Scanning Electron

ABSTRACTSpinal ligaments were obtained from normal and scoliotic individuals. Detailed morphological studies were carried out on the yellow, interspinous and supraspinous ligaments by light and scanning electron microscopy. Normal yellow ligaments are mostly constituted of dense elastin fibers with only a few collagen fibers and sparse blood vessels, while in normal interspinous and supraspinous ligaments, the presence of collagen is highly dominant. In the latter structures, the collagen fascicles are characterized by a regular waviness morphology. The fibrils constituting the fascicles appear either parallel or helical with respect to the fascicle axis. Structural changes are observed in the spinal ligaments of patients with congenital as well as idiopathic scoliosis. For yellow ligaments, only slight differences are found between normal and scoliotic specimens. However, alterations in collagen waviness and architecture are observed mainly in the supraspinous ligaments and to a lesser extent in the interspinous ligaments. In addition, increases in the cellularity, loose connective tissue and vessels are found in both forgoing ligaments. These results indicate that the more pronounced scoliosis-related changes occur in ligaments having the farthest distance from the axis of flexion-rotation.

Vasculitis

2018 ◽  
Author(s):  
Raashid Luqmani
Keyword(s):  
Connective Tissue ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Vessel Wall ◽  
Connective Tissue Diseases ◽  
Blood Vessel Wall ◽  
Target Organs ◽  
Laboratory Features ◽  
Life Threatening ◽  
Pulmonary Capillaritis

The vasculitides are a heterogeneous group of disorders that can range from mild inflammation of blood vessels in the skin, to organ- and life-threatening diseases. The term ‘vasculitis’ is a pathological description of blood vessel wall inflammation which leads to ischaemia and infarction of the target organs. Definitions and classifications of the primary vasculitides are mainly based on the predominant calibre of the blood vessels involved but incorporate clinical, pathological, and laboratory features. The secondary vasculitides usually occur in the context of other connective tissue diseases and are not discussed further in this section. Goodpasture’s disease is not usually included in the primary vasculitides, but has compatible clinical features of pulmonary capillaritis and glomerulonephritis.

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