VIABILITAS RHIZOBIUM DALAM FORMULA BAHAN PEMBAWA DAN CARA INOKULASI DALAM TEKNIK PRODUKSI MASSAL  PUPUK HAYATI

ABSTRAKTanaman kedelai dapat memfiksasi nitrogen dengan bantuan bakteri bintil akar yaitu bakteri Rhizobium sp. Pemanfaatan Rhizobium sp sebagai inokulan pupuk hayati sangat mendukungpeningkatan produktivitas kedelai. Inokulasi biji kedelai dengan Rhizobium spdapat membantu penyediaan N tanah untuk kedelai. Formula pupuk hayati yang terdiri atas 6 konsorsia isolat Rhizobium sp telah diuji di beberapa lokasi pada tanah masam menunjukkan hasil yang positif, sehingga untuk pengembangannya perlu dilakukan “produksi massal”. Penelitian teknik produksi massal dilakukan di “Pilot Plant Produksi Pupuk Hayati” Balai Penelitian Tanah di Bogor. Penelitian disusun dengan rancangan acak lengkap, perlakuan terdiri atas kombinasi antara cara inokulasi dan pengemasan dengan formula bahan pembawa. Perlakuan terdiri atas delapan kombinasi perlakuan diulang tiga kali. Pertumbuhan sp sebelum diinokulasi ke dalam bahan pembawa diamati. Hasil penelitian menunjukkan bahwa isolat Rhizobium sp yang digunakan mempunyai fase pertumbuhan berkisar antara 4–48 jam. Populasi Rhizobium dalamformula pupuk hayati yang diinokulasi ke dalam bahan pembawa Biochar+Gambut mampu mempertahankan populasi Rhizobium tertinggi hingga masa simpan 6 bulan dibandingkan perlakuan lainnya. Populasi Rhizobium pada perlakuan tersebut sebanyak8,13 log CFU.g -1 . Sedangkan populasi inokulan Rhizobium sp dalam pupuk hayati yang diinokulasikan menggunakan mesin injektor dengan bahan pembawa Biochar sebanyak 7,66 log CFU.g -1 .ABSTRACTSoybean plants can fix nitrogen with the help of root nodules bacteria, namely Rhizobium sp. The use of Rhizobium sp as an inoculant of biofertilizers supports to increase the productivity of soybean. Inoculation Rhizobium sp of soybean seeds helps to provide soil N for soybeans. Biofertilizer formula consist of six consortia of Rhizobium sp had been tested in more locations on acid soils showed positive results, so for it’s development for biofertilizer it is necessary to do "mass production", was conducted at the "Biofertilizer Production Plant Pilot" of Indonesian Soil Research Institute. The study was arranged in a completely randomized design, the treatment consist of ways of packaging methods and the carrier formula. The treatment consisted of eight combinations repeated three times. Rhizobium sp growth before inoculation into the carrier was observed. Rhizobium population in the formula of biofertilizer which is inoculated into the Biochar+Peat carrier and then packaged by packaging machine is able to maintain the highest Rhizobium population up to a shelf life of 6 months compared to other treatments,. Rhizobium sp population in this treatment was 8.13 logCFU.g -1 . While the population of Rhizobium sp inoculants in biofertilizers inoculated using an injector with Biochar carrier as much as 7.66 log CFU.g -1.

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Dosis dan Waktu Aplikasi Boron Terhadap Pertumbuhan dan Hasil Kacang Hijau

Agrosains: Jurnal Penelitian Agronomi ◽

10.20961/agsjpa.v22i2.42058 ◽

2020 ◽

Vol 22 (2) ◽

pp. 64

Author(s):

Krisna Cahya Nurani ◽

Susilo Budiyanto ◽

Endang Dwi Purbajanti

Keyword(s):

Plant Height ◽

Seed Weight ◽

Root Nodules ◽

Fertilizer Application ◽

Green Beans ◽

Great Demand ◽

Randomized Design ◽

Completely Randomized Design ◽

Legume Plants ◽

Generative Phase

<p>Green beans are legume plants that are in great demand because of their high nutrition content and include plants that require boron to support growth from the vegetative to generative phase. The study aims to assess the growth and production of green beans due to influence of the dose and time of boron application. The research was carried out using completely randomized design with factorial pattern 3×3 with 3 replications. The first factor was the treatment of boron doses of 1, 1.5, and 2 kg/ha was given once at 7 Day After Planting (DAP), fertilizer application twice at 7 and 28 DAP, and once given at 28 DAP. The results showed that dose of boron at a dose of 1 kg/ha was able to produce the number of affective root nodules and the best pod weight. The treatment of boron once at 7 DAP can increase plant height, flowering age, pod weight, and seed weight per pod. The interaction between dose and time of boron application affects the number of affective root nodules and seed weight per pod.</p>

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Efek Dolomit Dan Sp-36 Terhadap Bintil Akar, Serapan N Dan Hasil Kacang Tanah (Arachis hypogaea L.) Pada Tanah Kambisol

Agrologia ◽

10.30598/a.v1i2.283 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Charles Silahooy

Keyword(s):

Root Nodules ◽

N Uptake ◽

Phosphate Fertilizer ◽

Randomized Design ◽

Arachis Hypogaea L ◽

Two Factors ◽

National Production ◽

Peanut Production ◽

Completely Randomized Design ◽

Fertilizer Combination

Maluku in general and Ambon municipality in particular is a region with the lowest peanut production as compared to its national production. Cambisol is one of soil type that has good soil physical properties, but it is low in N and P contents and has an acidic reaction that inhibit the growth of peanut plants. The research was conducted in a plastic house in Halong Village, Teluk Ambon Baguala Subdistrict, from April to Juli 2012. This research used a Completely Randomized Design with two factors, i.e. factor 1 with three levels of dolomite fertilization, consisting of D0 (without dolomite), D1 (0.3g dolomite per pot), D2 (0.6 g dolomite per pot), and factor 2 with three levels of phosphate fertilization consisting of P0 (without phosphate fertilizer), P1 (0.7 g SP-36 per pot) and P2 (1.4 SP-36 per pot). The results showed that the application of SP-36 increased the number of root nodules and yield of peanut. Response was even better if accompanied by the application of dolomite. Leaf N uptake could be enhanced by application SP-36, but it was independ from the application of dolomite. The highest peanut yield of 41.83 g per pot was achieved with the fertilizer combination of D1P2 (0.3 g dolomite per pot and 1.4 g SP36 per pot)

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Potensi tepung daun sirsak, serai, kenikir, jeruk nipis, dan kluwih terhadap kumbang bubuk kedelai (Callosobruchus analis F.) pada kedelai (Glyxine max L.) dalam simpanan

Jurnal Proteksi Tanaman Tropis ◽

10.19184/jptt.v1i2.17918 ◽

2020 ◽

Vol 1 (2) ◽

pp. 55

Author(s):

Laily Dwi Dzulhijja ◽

Wagiyana W ◽

Sigit Prastowo

Keyword(s):

Seed Weight ◽

Probit Analysis ◽

Soybean Seeds ◽

Seed Damage ◽

Randomized Design ◽

Effect Of Treatment ◽

Completely Randomized Design ◽

The Difference ◽

Short Time ◽

Observation Results

This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. analis imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1 g, 2 g, and 3 g; C) Serai leaf flour with doses of 1 g, 2 g, and 3 g; D) kenikir leaf flour with doses of 1 g, 2 g, and 3 g; E) lime leaf flour with doses of 1 g, 2 g, and 3 g; and F) kluwih leaf flour with doses of 1 g, 2 g, and 3 g. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Tukey HSD Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3 g on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. analis the best analyst was the treatment of soursop leaf flour dose 3 g on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3 g at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3 g, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 g. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3 g because it could kill 50% of the population C. analis with a relatively short time were 9 days after treatment.

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Physiological potential of soybean seeds over storage after industrial treatment

Journal of Seed Science ◽

10.1590/2317-1545v40n3185104 ◽

2018 ◽

Vol 40 (3) ◽

pp. 272-280 ◽

Cited By ~ 2

Author(s):

Lucas Caiubi Pereira ◽

Mayara Mariana Garcia ◽

Alessandro Lucca Braccini ◽

Gláucia Cristina Ferri ◽

Andreia Kazumi Suzukawa ◽

...

Keyword(s):

Seed Germination ◽

Seedling Emergence ◽

Accelerated Aging ◽

Storage Period ◽

Soybean Seeds ◽

Fertilizer Use ◽

Thiophanate Methyl ◽

Pesticide Mixtures ◽

Randomized Design ◽

Completely Randomized Design

Abstract: The aim of this study was to evaluate the effect of industrial seed treatments on the physiological potential of soybean seeds over storage. Four mixtures of agrochemical products in association with two fertilizers were tested. The agrochemical product mixtures were carbendazim/thiram + imidacloprid/thiodicarb; pyraclostrobin, thiophanate-methyl, and fipronil; thiophanate-methyl/fluazinam + bifenthrin/imidacloprid; and metalaxyl-m/fludioxonil + thiamethoxam. The two fertilizers were 7% N, 16% P2O5, 0.6% Co, and 2.5% Mo; and 1% Co, 10% Mo, and 7% P2 O5. The experiment was carried out in a completely randomized design in a split-plot arrangement in time, with four replications. The treatments were allocated in the plots, while the storage periods (0, 30, 60, 90, and 120 days) constituted the split-plots. The following tests were carried out in each period: first count of germination, germination, accelerated aging, emergence speed index in sand substrate, and final seedling emergence in sand substrate. Seed germination and vigor declined over the storage period, especially after industrial treatment. Pesticide mixtures of a carbendazim/thiram fungicide base and an imidacloprid/thiodicarb insecticide base most impaired seed physiological potential throughout storage, regardless of fertilizer use in the industrial treatment.

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Organic Fungicides with Adjuvants in the Prevention of Diseases at Wheat

Journal of Agricultural Science ◽

10.5539/jas.v9n12p223 ◽

2017 ◽

Vol 9 (12) ◽

pp. 223

Author(s):

Silvio D. Ferreira ◽

Adriana C. Salvalaggio ◽

Jaqueline de A. Barbosa ◽

Neumarcio V. da Costa

Keyword(s):

Experimental Design ◽

Vegetable Oil ◽

Lower Incidence ◽

Mineral Oil ◽

Adaxial Side ◽

Organic Systems ◽

Randomized Design ◽

Completely Randomized Design ◽

Wheat Leaves ◽

Positive Results

The efficiency of fungal solutions, without wheat in organic systems, can be improved with the use of adjuvants. The objective of this study was to evaluate the dispersion of fungal droplets of organic fungicides with adjuvants in wheat leaves and disease control efficiency. The experimental design used was the completely randomized design in factorial scheme 2 × 3, with 6 repetitions. The first factor corresponded to fungal solutions bordeaux syrup (200 g L-1) and lime sulfur syrup (200 g L-1) and second factor to the adjuvants: mineral oil and vegetable oil (0.5% v v-1) and one Witness without application.. On the adaxial side, the use of the mineral oil provided scatter of drop 111.8 and 25.7% higher, to the grouts without adjuvants and vegetable oil, respectively. The greater spreading of the droplets on the two foliar faces and a lower incidence of the diseases with the product of the lime sulfur syrup + mineral oil. The use of the adjuvants in the syrups evaluated with positive results in the production components, standing out the mineral oil.

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Growth Response of Soybean Varieties to Trichoderma Application on Acid Soils

E3S Web of Conferences ◽

10.1051/e3sconf/202131603007 ◽

2021 ◽

Vol 316 ◽

pp. 03007

Author(s):

Sutarman ◽

A. Miftahurrohmat

Keyword(s):

Growth Response ◽

Acid Soils ◽

Dry Weight ◽

Randomized Design ◽

Affected Plant ◽

Significant Difference ◽

Wet Weight ◽

Completely Randomized Design ◽

Difference Test ◽

Honest Significant Difference Test

This study aims to determine the growth response of the vegetative stadia of three soybean varieties which are widely used in East Java on acid soils that have been given biological fertilizers from various Trichoderma isolates. The experiment was arranged factorially using a completely randomized design. The first factor was the variety consisting of Detam 3, Detam 4, Gema, Dering 1, and Burangrang. The second factor was Trichoderma isolates formulated as bio fertilizers, consisting of Tc-Jjr-02, Tc-Pjn-01, and Tc-Jro-01. Overall this experiment has 60 experimental units. Data were analyzed by variance analysis (ANOVA) followed by an honest significant difference test at the level of 5%. The results showed that there was no interaction between soybean varieties and Trichoderma isolates formulated in bio fertilizer. Soybean varieties significantly affected plant height, wet weight, and dry weight of roots and stover at 21 days after planting. The Burangrang variety shows the best growing ability in acid soils. There was no interaction between soybean varieties and Trichoderma bio fertilizers. Trichoderma isolate Tc-Jjr-02 increased the wet weight and dry weight of the roots and roots by 48.2 and 54.5%, respectively, and 38.9 and 48.2% compared to without Trichoderma. Trichoderma application maintains soil acidity between pH 4.50-4.67.

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The effect of replacing maize with re-binding banana hump flour on the meat quality, footpad color, and feather or hybrid duck

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/883/1/012059 ◽

2021 ◽

Vol 883 (1) ◽

pp. 012059

Author(s):

O Sjofjan ◽

D N Adli ◽

R H Syahputra

Keyword(s):

Field Experiment ◽

Meat Quality ◽

Breast Meat ◽

Research Material ◽

Randomized Design ◽

Binding Data ◽

Completely Randomized Design ◽

Positive Results ◽

Range Test

Abstract The purpose of this study was to determine the deposition percentage of breast meat, leg colour, and feather weight of hybrid ducks. The research material was 100 hybrid ducks regardless of sex (age 21 days) Peking x Khaki Campbell. The method used was a field experiment using a completely randomized design (CRD) consisting of 5 treatments and four replications. Each replication of 5 hybrid ducks. The treatments used were T0: 20% corn, T1: 15% corn + 5% Banana Hump Flour re-binding, T2: 10% corn + 10% Banana Hump Flour re-binding, T3: 5% corn + 15% Banana Hump Flour re-binding, T4: 20% Banana Hump Flour re-binding. Data were analyzed statistically using Covarian analysis (ANCOVA). If the data results differ significantly between treatments, Duncan's Multiple Range Test (DMRT) will proceed. The results showed that banana hump flour had no significant effect (P> 0.05) on the percentage of breast meat deposition and feather weight, but had a very significant effect (P < 0.01) on the color of the legs. It can be concluded that the use of re-binding banana hump flour as a substitute for corn up to 20% in feed gives positive results on the percentage of meat and feathers of hybrid.

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Uji Patogenesitas Oospora Peronospora manshurica Asal Kedelai Impor pada Tiga Kultivar Kedelai

Jurnal Media Pertanian ◽

10.33087/jagro.v6i1.112 ◽

2021 ◽

Vol 6 (1) ◽

pp. 23

Author(s):

Ani Ardiana Susanti ◽

Marlina Marlina ◽

Husda Marwan ◽

Mapegau Mapegau

Keyword(s):

Downy Mildew ◽

Incubation Period ◽

Disease Severity ◽

Class I ◽

Experimental Unit ◽

Soybean Seeds ◽

Soybean Cultivars ◽

Randomized Design ◽

Completely Randomized Design ◽

Soybean Plants

Downy mildew in soybean plants is caused by the fungus Peronosporamanshurica. This fungus is an obligate parasite and its existence in Indonesia is still limited. This study aims to determine the pathogenicity of the fungus P. manshurica carried on imported seeds of soybeans from Malaysia. The Oospora from imported soybean seeds from the Class I Agricultural Quarantine Center was tested for its pathogenicity on soybean cultivars Anjasmoro, Grobogan, and Wilis in the greenhouses of the Jambi Class I Agricultural Quarantine Center. This test used a completely randomized design (CRD), using 4 treatments; 3 superior soybean cultivars (Anjasmoro, Grobogan, and Wilis),and 1 imported soybean cultivar. Each treatment was repeated 6 times so that there were 24 experimental units and each experimental unit consisted of 5 plants so that the total number was 120 plants. The results showed that the pathogenicity of the Oospore ofP.manshurica from imported soybean seeds still caused downy disease in the three soybean cultivars tested. There was no difference in the incubation period of downy mildew caused by the Oospora ofP.manshurica on Anjasmoro, Grobogan, and Wilis cultivars, which were 5.5, 8.0, and 7.0 days after inoculation, respectively. The highest disease severity was found in the Anjasmoro cultivar (8.91%) and the lowest was in the Wilis cultivar (2.66%), but it was not different from the disease severity in the Grobagan cultivar (4.28%).

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Characteristic and boactive potential of brewed Sargassum sp. with the additional bay leaf (Syzygium polyanthum)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/890/1/012044 ◽

2021 ◽

Vol 890 (1) ◽

pp. 012044

Author(s):

A N Damayanti ◽

P H Riyadi ◽

E N Dewi

Keyword(s):

Consumer Acceptance ◽

Bioactive Components ◽

Flavonoid Content ◽

Fishy Odor ◽

Parametric Data ◽

Randomized Design ◽

Significant Difference ◽

Parametric Testing ◽

Completely Randomized Design ◽

Positive Results

Abstract Brewed Sargassum sp. is a product of dried seaweed that is packaged in tea bags and then brewed. The natural fishy odor from seaweed itself makes consumer acceptance of this brewed drink relatively low. The purpose of this study was to determine the effect of the addition of bay leaves (Syzygium polyanthum) and to determine consumer acceptance using hedonic test for Sargassum sp. Samples were obtained from UD Seaweed Mandiri, Wonosari, Yogyakarta. The research method used is experimental laboratories with a completely randomized design (CRD) model. The treatment given was in the form of different concentrations of bay leaves, namely 0%, 10%, 20%, and 30%. Parameters observed were water content, ash content, image processing, hedonic, phytochemical, antioxidant activity, and bioactive components. Parametric data were analyzed using analysis of variance ANOVA and Honest Significant Difference to determine differences between treatments, while non-parametric testing included hedonic tests consisting of aroma and taste tests. The results showed that the different concentrations of bay leaves gave a significantly different effect on the hedonic parameters. Brewed Sargassum sp. with a concentration of 30% had the highest score on the hedonic test, namely 7.71 < µ < 8.11 which was included as favored by the panelists. The addition of bay leaves with a concentration of 30% resulted in an IC50 value of 13.6251 ppm. Phytochemical screening which was carried out qualitatively showed positive results for the parameters of flavonoids, phenols, tannins, and saponins. The total flavonoid content of 30% concentration is 302,333 mg QE/g and produces six compounds with bioactive components. The highest compound is pentane at 69.12%. Based on the test of bioactive components in the brewed Sargassum sp. there is a pentane compound which has the potential as an antimicrobial and anti-inflammatory.

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PERTUMBUHAN DAN HASIL TANAMAN KEDELAI (Glycine max L.) TERHADAP PEMBERIAN VERMIKOMPOS DAN BIOCHAR DI TANAH ULTISOL

Jurnal Solum ◽

10.25077/jsolum.17.2.29-41.2020 ◽

2020 ◽

Vol 17 (2) ◽

pp. 29

Author(s):

Elisa Ester Sirait ◽

Nelvia Nelvia ◽

Hafiz Fauzana

Keyword(s):

Glycine Max ◽

Agricultural Production ◽

Crop Production ◽

Root Nodules ◽

Plant Age ◽

Growth And Yield ◽

Soybean Plant ◽

Randomized Design ◽

Glycine Max L ◽

Completely Randomized Design

The use of organic matter in agricultural production is important in increasing crop production, especially in Ultisols. This study aimed to study the interaction between vermicompost and biochar on the growth and yield of soybean (Glycine max L.) in Ultisols. The research was carried out in the experimental station of the Faculty of Agriculture, Riau University, Bina Widya Tampan campus, Pekanbaru from July - September 2018. The research used factorial design form in a completely randomized design. The first factor is vermicompost which consists of 4 levels (doses of 0, 25, 50 and 75 g of polybag-1) while the second factor is biochar consisting of 4 levels (doses of 0, 25, 50 and 75 g of polybag-1) for each combination repeated 4 times. The parameters observed were the number of effective roots, proportion of effective nodules, plant height, number of primary branches, age of plants, age of harvest, proportion of well-fused pods, number of seeds, seed weight and weight of 100 seeds. The results showed that the interaction of vermicompost at a dose of 25 g.polybag-1 following a dose of 50 g.polybag-1 biochar increased the number of effective root nodules, the proportion of effective root nodules, the number of primary branches, the proportion of pithy pods, and accelerated plant age and soybean plant age compared without treatment but not significantly compared to other combinations.Key words : Soybean, vermicompost, biochar

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