scholarly journals Anti-Hypertensive and Anti-Oxidative Effect of Methanolic Extract of Vernonia Amygdalina (VA) in Tissue Samples of NaCL Induced Hypertensive Male Wistar Rats

2018 ◽  
Vol 7 (5) ◽  
Author(s):  
Onyema- Iloh OB ◽  
Meludu SC ◽  
Iloh EO ◽  
Dioka CE
Keyword(s):  
Wistar Rats ◽  
Methanolic Extract ◽  
Vernonia Amygdalina ◽  
Tissue Samples ◽  
Male Wistar Rats ◽  
Oxidative Effect

Comparing the Neuroprotective Effects of Aqueous and Methanolic Extracts of Vernonia Amygdalina on the Cerebellum of Adult Male Wistar Rats

2020 ◽  
Vol 9 (1) ◽  
pp. 145-159
Author(s):  
Ugochukwu Samuel Aguwa ◽  
Somadina M Okeke ◽  
Chijioke M Okeke ◽  
Chinyere S Eze ◽  
Benedict Nzube Obinwa ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Adult Male ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Mercury Chloride ◽  
Phytochemical Analysis ◽  
Vernonia Amygdalina ◽  
Orthodox Medicine ◽  
Methanolic Extracts ◽  
Male Wistar Rats

The solvent of extraction determines the type and amount of phytochemicals extractible from a plant material. Reports have shown that numerous African herbs are rich is potent phytochemicals which have been used in folkloric and orthodox medicine in the prevention and treatment of ailments. This research is geared towards comparing the neuroprotective potency of the aqueous and methanolic extracts of Vernonia amygdalina on the cerebellum of mercury chloride intoxicated Wistar rats. Thirty adult male Wistar rats were divided into 6 groups of 5 rats each. Group A was the negative control and had food and water only. Group B was the positive control and was exposed to mercury chloride without treatment. Groups C1 and C2 received 200mg/kg bw of aqueous and methanolic extracts respectively while groups D1 and D2 received 400mg/kg bw of aqueous and methanolic extracts of Vernonia amygdalina respectively. Result of phytochemical analysis shows that water extracted more flavonoids while methanol extracted more phenols. The results of antioxidant studies show that the methanolic extract conferred more protection against oxidative stress than the aqueous extract. Histological results show that aqueous extract conferred more protection than the methanolic extract. We therefore conclude from our report that both the aqueous and methanolic extracts of Vernonia amygdalina confer neuroprotection on the cerebellum of Wistar rats.

scholarly journals Evaluating the Neuroprotective Effects of the Aqueous and Methanolic Extracts of Vernonia amygdalina on the Hippocampus of Adult Male Wistar Rats

2020 ◽  
pp. 1-16
Author(s):  
U. S. Aguwa ◽  
D. K. Ogbuokiri ◽  
C. S. Eze ◽  
B. N. Obinwa ◽  
F. O. Ovie ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Negative Control ◽  
Mercury Chloride ◽  
Phytochemical Analysis ◽  
Vernonia Amygdalina ◽  
Neuroprotective Effects ◽  
Methanolic Extracts ◽  
Male Wistar Rats

The phytochemical yield of a plant material is related directly to the method as well as solvent of extraction. Lots of African herbs have proven over time to be highly medicinal both for the prevention, management and cure of various ailments. The aim of this research is to compare the neuroprotective potency of the aqueous and methanolic extracts of Vernonia amygdalina on the hippocampus of mercury chloride intoxicated Wistar rats. Thirty male Wistar rats weighing between 180 to 200 g were divided into 6 groups of 5 rats each. Group A was the negative control and had food and water only. Groups B was the positive control and groups C1, C2, D1 and D2 were the test groups. Rats in group B to D2 were exposed to 0.5 mg/kg/b.w of mercury chloride two times a week for 2 weeks. Groups C1 and C2 were treated with 200 mg/kg/bw of aqueous and methanolic extracts respectively while groups D1 and D2 received 400 mg/kg/bw of aqueous and methanolic extracts of Vernonia amygdalina respectively. Qualitative and quantitative phytochemical analysis shows that water extracted more flavonoids while methanol extracted more phenols from the plant. The results of antioxidant studies show that the methanolic extract conferred more protection against oxidative stress than the aqueous extract. Neurobehavioural and histological results show that aqueous extract conferred more protection on the cells and tissue structure of the hippocampus than the methanolic extract. We therefore conclude that both the aqueous and methanolic extracts of Vernonia amygdalina confer some form of neuroprotection on the hippocampus of Wistar rats but the aqueous extract gave better results.

scholarly journals Hepatotherapeutic Tendency of Citrullus lanatus Rind Methanolic Extract on Liver Markers in Male Wistar Rats

2019 ◽  
pp. 1-10
Author(s):  
Ilochi Ogadinma ◽  
Chuemere Arthur Nwafor ◽  
Bassy Samuel
Keyword(s):  
Liver Function ◽  
Wistar Rats ◽  
Liver Enzymes ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Left Ventricular ◽  
Normal Male ◽  
High Dose ◽  
Serum Total Bilirubin ◽  
Male Wistar Rats

It is a common practice to dispose the peel or rind of fruits. Interestingly, some parts of fruits humans find unacceptable to eat actually possess bioactive nutrients that can be used for medicinal purposes.  The effect of methanolic extract of Citrullus lanatus rind on liver function in normal male wistar rats was studied. 24 wistar rats with body weight between 150-250 g were used for this study. The animals were randomly divided into four groups, with 6 rats in each. Group 1 contained the control given normal saline and feed; group 2, low dose, 50mg/kg of methanolic extract of  Citrullus lanatus rind was administered, group 3 and 4 were administered medium and high dose of Citrullus lanatus rind extract 100 mg/kg and 200 mg/kg respectively. The Citrullus lanatus rind extract was administered via orogastric route and the experiment lasted for a period of 56 days. Blood samples were collected by left ventricular cardiac puncture for liver function test at the last day of the experiment.  The outcome of this research revealed that medium and high dose administration of citrullus lanatus rind significantly (p≤0.05) reduced the serum level of liver enzymes alanine transaminase, aspartate transaminase and alkaline phosphatase and also total protein.There was a non-significant (P≤0.05) change in serum total bilirubin and albumin when all doses were compared to the control. Prolonged and moderate ingestion of Citrullus lanatus rind may be of benefit in regulating blood level of liver enzymes; hence, this part of the fruit has therapeutic value.

Toxicological evaluations of methanolic extract of Moringa oleifera leaves in liver and kidney of male Wistar rats

2013 ◽  
Vol 24 (4) ◽  
Author(s):  
Ademola A. Oyagbemi ◽  
Temitayo O. Omobowale ◽  
Ibraheem O. Azeez ◽  
John O. Abiola ◽  
Rahamon A.M. Adedokun ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Methanolic Extract ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Moringa Oleifera Leaves

scholarly journals Preliminary Study on the Effect of Methanolic Extract of Watermelon (Citrullus lanatus) Rind on Prednisolone Suppressed Immunity in Male Wistar Rats

2021 ◽  
pp. 1-6
Author(s):  
Nyejirime Young Wike ◽  
Mobisson Samuel Kelechi ◽  
Godspower Onyeso ◽  
Okekem Amadi ◽  
Elizabeth Eepho Krukru
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Methanolic Extract ◽  
Citrullus Lanatus ◽  
Control Group ◽  
Group 4 ◽  
Watermelon Rind ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Citrullus lanatus thumb (Cucurbitaceae) commonly called watermelon is widely consumed in this part of the world as food and medicine. This study was carried out to examine the effect of methanolic extract of watermelon (Citrullus lanatus) rind on prednisolone suppressed immunity in male wistar rats. A total of 20 male wistar rats weighing 150-294g were used in 4 groups with five rats each. Group 1, the control group was given distilled water and feed, Group 2 was given 200 mg/kg body weight of methanolic extract of watermelon rind, Group 3 rats were given 2.5 mg/kg body weight of prednisolone and Group 4 rats were given 2.5 mg/kg body weight of prednisolone and 200 mg/kg body weight of methanolic extract of watermelon rind. Prednisolone and the methanolic extract of watermelon rind were administered orally for a period of 30 days. Blood samples were collected by cardio puncture from the rats for white blood cell (WBC), lymphocyte, and granulocyte and monocyte counts at the end of the experiment.  The data were statistically analysed using one-way ANOVA (Analysis of variance). Data were considered significant at p<0.05. The results obtained showed that methanolic extract of watermelon rind caused a significant increasen in immune function of rats when compared with the control and immune suppressed rats.

scholarly journals Ameliorative Effect of Trevo Dietary Supplement Against Lead Acetate Nephrotoxicity

2022 ◽  
Vol 16 (1) ◽  
pp. 34-42
Author(s):  
Omotayo B. Ilesanmi ◽  
◽  
Eni-yimini Solomon Agoro ◽  
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Antioxidant Properties ◽  
Kidney Tissue ◽  
Lead Toxicity ◽  
Serum Levels ◽  
Tissue Samples ◽  
Male Wistar Rats

Background: Trevo is a nutritional supplement with numerous bioactive natural products, with detoxifying and antioxidant properties. The purpose of this study was to investigate the ability of Trévo to protect against oxidative stress induced by lead in the kidneys of male Wistar rats. Methods: Thirty-five healthy male Wistar rats were divided into five groups of seven rats each, using a randomized design. I=control; II=15 mg/kg of lead acetate (PbA); III=2 ml/kg of trevo+PbA; IV=5 ml/kg of trevo+PbA; V=5 ml/kg of trevo. Animals were treated with trevo for five days before co-administration with lead intraperitoneally for 10 consecutive days. Animals were sacrificed 24hr after the last administration, blood samples were collected via cardiac puncture, and processed for assessment of urea, creatinine, and uric acid (UA), while the kidney samples were excised and processed for the following biochemical assays: Malondialdehyde (MDA), Glutathione-S-Transferase (GST), Catalase (CAT), Superoxide Dismutase (SOD), and Reduced Glutathione (GSH). Results: Injection of PbA caused a significant increase in the serum levels of urea, creatinine, and uric acid, and a significant increase (P<0.001) in the MDA concentration, and decreases in GSH concentration, CAT, SOD, and GST activities (P<0.05) as compared to the controls. Pretreatment with trevo prevented the oxidative stress induced by lead acetate in the kidney tissue samples and improve the renal function. The protective effect was evident at 5 ml/kg of trevo. Conclusion: The results showed that trevo was nephroprotective against lead toxicity and the activity might be linked to the presence of numerous antioxidant phytochemicals present in trevo.

Sign in / Sign up

Export Citation Format

Share Document