scholarly journals The biotechnological studies when developing lozenges with probiotics

2021 ◽  
pp. 38-43
Author(s):  
U. Starushenko ◽  
L. Yarova ◽  
O. Kaliuzhnaia ◽  
N. Khokhlenkova ◽  
O. Kaliuzhnyi
Keyword(s):  
High Efficiency ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Bacterial Survival ◽  
Active Components ◽  
Growth Inhibition Zone ◽  
Treatment And Prevention ◽  
Probiotic Strains ◽  
Viable Bacteria

Aim. To develop lozenges with probiotic components for the treatment and prevention of ENT diseases and analyze their effectiveness.Materials and methods. The study objects were lozenges based on xylitol and sorbitol of two compositions – based on live probiotic cultures and their metabolites. The following methods of control of the sample efficiency werechosen: determination of the number of viable lactobacilli in the study of their combined use with the drug components and the number of viable bacteria in finished oral probiotics by the Koch method (direct seeding on cups), as well as the method of diffusion into agar with the growth inhibition zone determination.Results and discussion. The studies of the properties of probiotic strains of L. fermentum 90-TC and L plantarum 8P-A3 have shown the prospects for their use in oral probiotics. It has been also proposed to use metabolitesof these strains as active components, which have a rather high antimicrobial potential to opportunistic pathogens that cause infectious ENT diseases. The oral probiotics in the form of lozenges of two compositions – based on live lactobacilli and their metabolites – have shown high efficiency, bacterial survival and antimicrobial properties, respectively.Conclusions. During the complex of the theoretical, technological and biotechnological studies, lozenges with a probiotic of two compositions (based on viable probiotic cultures and their metabolites) have been developed. After further research they can be recommended as a biologically active additive to support the oral microflora, stimulate the own immunity and as a therapeutic and prophylactic agent in infectious diseases of the oral cavity for use in both adults and children.

scholarly journals Evaluation of the Effect of Propolis Nanoparticles on Antimicrobial Properties and Shear Bond Strength of Orthodontic Composite Bonded to Bovine Enamel

2019 ◽  
Author(s):  
Ahmad Sodagar ◽  
Azam Akhavan ◽  
Sepideh Arab ◽  
Abbas Bahador ◽  
Maryam Pourhajibagher ◽  
...  
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Antimicrobial Property ◽  
Testing Machine ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Colony Growth ◽  
Control Group ◽  
Growth Inhibition Zone ◽  
Bovine Enamel

We aimed to investigate the effects of propolis nanoparticles (prpNPs) on antimicrobial property and shear bond strength (SBS) of orthodontic composite bonded to bovine enamel. Materials and Methods: Sixty bovine teeth were randomly divided into five groups (n=12). PrpNPs were prepared at concentrations of 0% (control), 1%, 2%, 5%, and 10% in Transbond XT composite to bond stainless steel brackets to the teeth. SBS between brackets and teeth was measured using a universal testing machine. After debonding, the adhesive remnant index (ARI) on bracket bases was measured. In the microbial test, composites with the aforementioned concentrations of prpNPs were cured in metal discs. The bacteria included Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), and Lactobacillus acidophilus (L. acidophilus), and antimicrobial effects of prpNPs were investigated by anti-biofilm, disc agar diffusion and eluted component tests. Results: The 10% prpNPs group showed the lowest SBS. Colony growths of S. mutans and S. sanguinis at all concentrations (except for 1%) was significantly lower than the control group. L. acidophilus colony growth was significantly reduced at 5% and 10% concentrations. Growth inhibition zone developed at 2%, 5%, and 10% concentrations for S. mutans and S. sanguinis. The lowest numbers of S. mutans and S. sanguinis colonies at all concentrations were observed on day 15. L. acidophilus colonies decreased significantly at all concentrations (except for 1%) until day 30.

scholarly journals Antimicrobial properties, anti-virulence activities, and physico-mechanical characteristics of orthodontic adhesive containing C-phycocyanin: a promising application of natural products

Folia Medica ◽  
2021 ◽  
Vol 63 (1) ◽  
pp. 113-121
Author(s):  
Maryam Pourhajibagher ◽  
Abbas Bahador
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Antimicrobial Properties ◽  
Acrylic Resin ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Microbial Biofilms ◽  
Growth Inhibition Zone ◽  
Orthodontic Adhesives ◽  
Acrylic Resins

Introduction: Antimicrobial orthodontic adhesives aim to reduce enamel demineralization, white spot lesions, and incipient tooth decay around bonded orthodontic brackets, but they should not imperil its mechanical properties.  Aim: To evaluate the antimicrobial and physico-mechanical properties of acrylic containing different concentrations of C-phycocyanin on Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Materials and methods: The mechanical properties of acrylic resins were measured by flexural strength test after preparation of acrylic resin samples with concentrations of 1%, 2%, 5%, 7.5%, and 10% of C-phycocyanin. Then we evaluated the antimicrobial effects of acrylic resin containing the maximum concentration of C-phycocyanin with clinically acceptable flexural strength and the changes in expression of virulence factors. Results: The highest and lowest means of flexural strength were obtained in acrylic resins containing 0% and 10% concentrations of C-phycocyanin at 50.2±4.5 and 30.1±3.3 MPa, respectively. Adding 1%, 2%, and 5% of C-phycocyanin showed no significant decrease in flexural strength (p>0.05). The maximum mean diameter of the growth inhibition zone was observed around discs containing 5% of C-phycocyanin. Until day 30 of the study, no microbial biofilms were formed on any acrylic disc. Only microbial biofilms of C. albicans were able to form on discs containing 5% of C-phycocyanin at 90 days. 5% C-phycocyanin could significantly decrease the expression levels of gtfB, hsp16, and ALS9 6.1-, 7.3-, and 3.9-fold, respectively. Conclusions: It can be concluded that the most acceptable concentration of C-phycocyanin in acrylic resin is 5% based on the results of flexural strength tests and antimicrobial activities of acrylic resin containing various concentrations of C-phycocyanin.

scholarly journals Antibacterial activity of different fractional extracts of cerastium glomeratum

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Himayat Ullah ◽  
Muhammad Hubaib ◽  
Muhammad Israr ◽  
Muhammad Mushtaq ◽  
Muhammad Zeeshan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Active Components ◽  
Gram Positive ◽  
Gram Negative ◽  
Soxhlet Apparatus ◽  
Wide Range

Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.

scholarly journals Thermal fields of the Ebeko volcano (Paramushir Island, Kuril Islands) and their recreational and tourist potential

2020 ◽  
Vol 4 (4) ◽  
pp. 514-525
Author(s):  
R.V. Zharkov ◽  
Keyword(s):  
Chemical Properties ◽  
Thermal Spring ◽  
Kuril Islands ◽  
Biologically Active ◽  
Thermal Waters ◽  
Active Components ◽  
The North ◽  
Treatment And Prevention ◽  
Mineralized Water ◽  
Wide Range

The paper provides with the research results of physicochemical and balneological properties of thermal waters and hydrothermal mud of the Ebeko volcano (Paramushir Island, Kuril Islands). To assess recreational and tourist potential, we sample the most intensive thermal waters outputs. Ultra-acidic spring no. 1 of the Verkhne-Yuryeva group has a temperature of 88 °C, its mineralized (M – 13 g/l) chloride-sulfate waters contain biologically active elements in elevated concentrations (Si, B, Br, Fe2 +). Thermal waters of the Verkhne-Yuryeva springs can be roughly attributed to the Gaisky type of the acid water group and recommended for external use in the treatment and prevention of a wide range of diseases. Given the complexity of the route to the springs, the real development of this territory is considered not as a balneotherapy complex, but as an object of recreational and tourist activities. A similar situation is with an ultra-acidic sulfate thermal spring in the North-Eastern solfataric field. In addition to hydrotherms, it contains thin deposits of hydrothermal mud , which is almost impossible to use as a therapeutic due to its insufficiently good physical indicators and relatively small volume. The most promising deep hydrotherms for recreation and balneotherapy are opened by a well P-2 in the area of Severo-Kurilsk. In 2014, the hydrotherms temperature was 82 °C, mineralized water (M – 8.6 g/l) chloride-hydrocarbonate sodium, slightly alkaline (pH 7.6), with an elevated content of biologically active components (Si, B, Br). According to the physical and chemical properties, the thermal waters of well P-2 can be tentatively attributed to the Lazarevsky hydrochemical type of therapeutic drinking water of chloride-hydrocarbonate sodium group, and as an external (balneological) use, they are tentatively close to the Kuldur type of siliceous thermal waters of various ion composition.

scholarly journals SEARCH FOR BIOLOGICALLY ACTUVE SUBSTANCES WITH ANTIMICROBIAL AND ANTIBIOTIC POTENTIATING PROPERTIES AMOUNG MEMBERS OF THE GENUS ARTEMISIA AGAINTS THE MAIN PATHOGENS OF INFECTIOUS SKIN LESIONS

2021 ◽  
pp. 84-89
Author(s):  
O. І. Yurchyshyn
Keyword(s):  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Skin Lesions ◽  
Synergistic Interaction ◽  
Artemisia Vulgaris ◽  
Active Components ◽  
Artemisia Dracunculus ◽  
Wide Range ◽  
Dose Dependent

Abstract. Active components of the genus Artemisia show a wide range of antimicrobial effect against most bacterial pathogens. In addition, artemisin isolated from wormwood is used to treat chloroquine-resistant malaria. Antimicrobial and antibiotic-potentiating effects of eight aqueous ethanolic extracts (40 %, 70 % and 90 %) aerial part of the genus Artemisia aqueous against S. aureus and S. epidermidis strains with various types of resistance to macrolides, lincosamides, tetracyclines and fluoroquinolones isolated from outpatients with different forms of pyoderma have been researched with agar diffusion micromethod. Determination of effective antimicrobial concentrations of antimicrobials and test extracts against staphylococci have been performed with serial dilutions micromethod. The statistical program "Statistica", computer programs UTHSCSA Image Tool 3.0 and Microsoft Office Excel 2016 have been used for statistical processing of microbiological research results. The pronounced antimicrobial effect of the wormwood extract Artemisia dracunculus L. (MIC 125.0 - 250.0 μg/ml) against all S. aureus and S. epidermidis test strains has been established. Other studied extracts showed much weaker antimicrobial effect (MIC 1000.0 - 1500.0 μg/ml). It was found that the species of staphylococci and the phenotype of resistance of test strains do not affect antimicrobial activity of the studied extracts. We have found that there is no clear сorrelation between antimicrobial properties of the studied extracts and the phenotype of resistance of staphylococci test-strains. Extracts were equally effective against staphylococcal strains with low level of resistance by efflux of antimicrobial drug and skin isolates with chromosomal resistance. The greatest resistance to BAC of the studied extracts was showed by test strains with a high level of resistance to antibiotics of MLS-group and tetracycline, exhibiting sensitivity only to tarragon wormwood. Active components of 70, 90 % common mugwort extract Artemisia vulgaris L. (increase of the inhibition zone up to 117 – 142 %, p<0.05) and southern wormwood extract (increase of the inhibition zone up to 50 – 59 % and 74 – 122 %, respectively, p<0.05) showed dose-dependent synergistic interaction with erythromycin. Common mugwort extract (70 %) showed synergistic interaction with ¼ MIC of tetracycline (increase of the inhibition zone up to 100 %) against strains with combined resistance to all studied antimicrobials. For the study we used crude total extracts of medicinal plants (40 %, 70 % and 90 % ethanol), so we expect their significantly higher antimicrobial effect against staphylococcal strains while optimizing the extraction process and subsequent purification. It should be noted that 90 % aqueous ethanol extracts showed significantly better antimicrobial properties compared to 40 % and 70 % extracts. Active compounds of tarragon wormwood Artemisia dracunculus L. extract show pronounced antimicrobial effect against S. aureus and S. epidermidis strains, the main causative agents of infectious skin lesions, with different types of resistance to macrolides, lincosamides, tetracyclines and fluoroquinolones. Dose-dependent synergistic interaction with macrolides of common mugwort Artemisia vulgaris L. extracts and southern wormwood Artemisia abrotanum L. extract with macrolides (erythromycin) and tetracycline has been revealed.

Bioactive peptides from egg: a review

2015 ◽  
Vol 45 (2) ◽  
pp. 190-212 ◽  
Author(s):  
Z. F. Bhat ◽  
Sunil Kumar ◽  
Hina Fayaz Bhat
Keyword(s):  
Bioactive Peptides ◽  
Antioxidant Activities ◽  
Positive Impact ◽  
Antimicrobial Properties ◽  
Specific Protein ◽  
Biologically Active ◽  
Content Type ◽  
Treatment And Prevention

Purpose – The aim of the article was to focus on various peptides identified in the egg and their probable application as novel ingredients in the development of functional food products. Bioactive peptides of egg origin have attracted increasing interest as one of the prominent candidates for development of various health-promoting functional and designer foods. Design/methodology/approach – Traditionally known as a source of highly valuable proteins in human nutrition, eggs are nowadays also considered as an important source of many bioactive peptides which may find wide application in medicine and food production. These specific protein fragments from egg proteins which, above and beyond their nutritional capabilities, have a positive impact on the body’s function or condition by affecting the digestive, endocrine, cardiovascular, immune and nervous systems, and may ultimately influence health. Findings – Several peptides that are released in vitro or in vivo from egg proteins have been attributed to different health effects, including antihypertensive effects, antimicrobial properties, antioxidant activities, anticancer activity, immunomodulating activity, antiadhesive properties and enhancement of nutrient absorption and/or bioavailability. Extensive research has been undertaken to identify and characterize these biologically active peptides of egg origin which has changed the image of egg as a new source of biologically active ingredients for the development of functional foods with specific benefits for human health and treatment and prevention of diseases. Originality/value – The paper mainly describes the above-stated properties of bioactive peptides derived from egg proteins.

scholarly journals EFEK ANTI JAMUR AIR RENDAMAN DAUN KELOR (Moringa oleifera) TERHADAP PERTUMBUHAN JAMUR Trichophyton mentagrophytes SECARA IN VITRO

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Jirna Inyoman
Keyword(s):  
Moringa Oleifera ◽  
Antimicrobial Properties ◽  
Test Group ◽  
Average Diameter ◽  
Trichophyton Mentagrophytes ◽  
Inhibition Zone ◽  
Growth Inhibition Zone ◽  
Post Test ◽  
Test Use

Abstract Background Moringa oleifera is a plant that all parts can be used for medicinal purposes. Roots, flowers, bark, stems, leaves and seeds of Moringa has antimicrobial properties. Compound content in leaves of moringa, such as tannin,steroid, alkaloid, saponins ,and flavonoids . ObjectiveThe purpose of this research was to know the different of Trichophyton mentagrophytes growth on various leaves of moringa (Moringa oleifera) concentration extract. Methods This research uses a true experimental with post test only kontrol group design. The antifungal activity test use diffusion disk method, these were divided into four test group with concentration 25%, 50%, 75%, 100% and two kontrol group were positive kontrol (ketokonazole 2%) and negatif kontrol (sterile distilled water). The result of this study showed that the average diameter of inhibition zone of concentration 25% until 100% is 0 mm. Statistic analysis was done by using Kolmogorof Smirnov test and Kruskal Wallis test. The test showed there was not a significant mean difference between group. All consentration haven’t an effect on Trichophyton mentagrophytes growth inhibition zone diameter. For next research with same topics using pure extract leaves of moringa was recommended. Keywords: Inhibition zone, Moringa oleifera, Trichophyton mentagrophytes.

scholarly journals Practical Recommendations of Scientific Society for the Study of Human Microbiome and the Russian Gastroenterological Association on Use of Probiotics, Prebiotics, Synbiotics and Functional Foods in Treatment and Prevention of Gastroenterological Diseases in Children and Adults

2021 ◽  
Vol 31 (2) ◽  
pp. 65-91
Author(s):  
V. T. Ivashkin ◽  
I. V. Maev ◽  
D. I. Abdulganieva ◽  
S. A. Alekseenko ◽  
A. V. Gorelov ◽  
...  
Keyword(s):  
Primary Care Physicians ◽  
Functional Foods ◽  
Food Additives ◽  
Human Microbiome ◽  
Biologically Active ◽  
Gastrointestinal Microbiota ◽  
General Internists ◽  
Treatment And Prevention ◽  
Practical Guidelines ◽  
Probiotic Strains

Aim. The practical guidelines are intended for primary care physicians, general practitioners, paediatricians, gastroenterologists and general internists to advance the treatment and prevention of gastroenterological diseases in adults and children in therapies with probiotics, prebiotics, synbiotics and their enriched functional foods.Key points. Probiotics are live microorganisms that sustain health of the host when supplied in adequate amounts. Prebiotics include human-indigestible but accessible to gut microbiota substances expediting specific changes in the composition and/or activity of gastrointestinal microbiota that favour the host health. The mechanism of probiotic action comprises the quorum resistance maintenance, nutrient substrate metabolism and end metabolite recycling, macroorganism-sustaining substrate production, as well as the mediation of local and adaptive immune responses.The Russian Federation regulates market differently for biologically active food additives (BAFA), medicinal products (drugs) and functional food products (FFP). We overview the probiotic strains regulated in Russia as BAFAs, drugs and FFPs and provide recommendations on the use of these strains in treatment and prevention of gastroenterological diseases in children and adults.Conclusion. The clinical efficacy of probiotics, prebiotics, synbiotics and fortified functional foods depends on the prebiotic and strain properties and is verified in appropriate comparative clinical trials. Not all probiotics registered in Russia as BAFAs, drugs and FFPs have a strain identity, which provides no warranty of the clinical effect expected. The FFP legislation demands improved regulation mechanisms and control for therapeutic efficacy.

scholarly journals Antibacterial activity of different fractional extracts of cerastium glomeratum

2020 ◽  
Vol 5 (2) ◽  
pp. 57-62
Author(s):  
Himayat Ullah ◽  
Muhammad Hubaib ◽  
Muhammad Israr ◽  
Muhammad Mushtaq ◽  
Muhammad Zeeshan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Inhibition Zone ◽  
Biologically Active ◽  
Active Components ◽  
Gram Positive ◽  
Gram Negative ◽  
Soxhlet Apparatus ◽  
Wide Range

Every plant available in the universe is not produced to be the part of biodiversity, but is also produced to obtain medicinal purposes. The medicinal value of the plant is due to the availability of various chemical compounds [1]. Since prehistoric time, the crude extract of plant is used to cure various diseases, although the biologically active components of the plant were unidentified [2]. In the present study the antibacterial activity is performed for various extracts of cerastium glomeratum. The plant cerastium glomeratum was collected from near kalpani river Mardan, Pakistan and was identified by lecturer Israr department of botany GPGC Mardan, Pakistan. The plant was dried under shade and grinded into powder form. The extraction process was done by using soxhlet apparatus, reflux condenser and maceration process using ethanol as a solvent. The extract was divided into different fractions of n-hexane, DCM, ethyl acetate and water. These four extracts were tested against gram positive (staphylococcus aureus) and gram negative (E.coli) bacteria using well diffusion technique. The water extract show a wide range inhibition zone against both gram positive and gram negative bacteria while n-hexane, DCM and ethyl acetate fractions show no zone of inhibition against bacteria. In effort to find new antibacterial compounds cerastium glomeratum seems to be a good plant for additional phytochemical studies.

scholarly journals Possibilities of using functional biologically active organosilicon compounds in veterinary practice

2020 ◽  
Vol 202 (11) ◽  
pp. 53-58
Author(s):  
A. Barkova ◽  
E. Shurmanova ◽  
Tat'yana Honina ◽  
I. Mil'shteyn
Keyword(s):  
High Efficiency ◽  
Inflammatory Diseases ◽  
Skin Lesions ◽  
Domestic Animals ◽  
Biologically Active ◽  
Control Group ◽  
Duration Of Treatment ◽  
Pharmacological Agents ◽  
Treatment And Prevention ◽  
Silicon Silicon

Abstract. Purpose. Evaluation of the possibility of using silicon, silicon-zinc, silicon-zinc organoboron compounds and compositions based on them for the treatment and prevention of inflammatory and non-inflammatory diseases in agricultural and small domestic animals. Method. There was carried out a clinical trial of various medicinal compositions with a base in the form of organic compounds of silicon, zinc, boron, both containing antimicrobial components and environmentally friendly agents. For this purpose, groups of animals were formed on the principle of analogs. The multiplicity and administration of drugs depended on the studied pathology. Results. The obtained results showed a high efficiency of the studied compositions, in particular, in the treatment of postpartum diseases of the uterus of an inflammatory nature, the effectiveness of the application was 95.5 %, with conservative treatment of the retention of the placenta – 100 %. Also, these funds are highly effective for the treatment of hyperkeratosis of the udder nipples, without changing the technology of milking. On average, the effectiveness of the compositions reached 86.1 % when the funds were used for 7 days. The study of an antimicrobial composition based on organosilicon glycerohydrogel in the treatment of purulent-necrotic lesions in the distal part of the extremities in cows can reduce the frequency of procedures and the duration of treatment by 6 days compared to animals in the control group. Scientific novelty. New functional biologically active pharmacological agents based on silicon-, silicon-zinc-, silicon-zinc boron glycerohydrogels have been developed and clinically tested for the treatment of cows with postpartum uterine diseases, in particular, postpartum endometritis and retention of the placenta, hyperkeratosis of the udder nipples, purulent-necrotic diseases of the distal also necrotic skin lesions of small domestic animals.

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