scholarly journals PERBANDINGAN EFEKTIVITAS BUAH STROBERI (Fragaria x ananassa) DENGAN BUAH JERUK NIPIS (Citrus aurantifolia) SEBAGAI BAHAN ALAMI PEMUTIH GIGI SECARA IN VITRO

2017 ◽  
Vol 5 (2) ◽  
pp. 129-135
Author(s):  
Nia Nurhaeni ◽  
Denas Symond ◽  
Bambang Ristiono
Keyword(s):  
Fragaria X Ananassa ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Tooth Whitening ◽  
Citrus Aurantifolia ◽  
Treatment Groups ◽  
Color Changes ◽  
Significant Difference ◽  
Lime Concentration

One of the aesthetic problem which had bother and become a complaint was teeth discoloration that can be overcome by dental bleaching procedures. The use of tooth whitening ingredient can cause side effects such as tooth sensitivity and mucous irritation. Therefore, many researchers have been looking for a safer alternatives materials  to be used as tooth whitening ingredients including Strawberry (Fragaria x ananassa) contain of elegat acid and malic acid and Lime (Citrus aurantifolia) contain of citric acid which have potential to whiten the teeth. The purpose of this research is to determine differences strawberry and lime fruit as a natural ingredient of tooth whitening. This research used in vitro laboratory experiment method by using 30 post-extraction premolar on teeth divided into three groups, namely the treatment of strawberry concentration of 100% (K1), the treatment group lime concentration of  2.5% (K2 ) and the treatment control group carbamide peroxide 10% (K3). Color changes measurements was observed pretest and posttest by 15 observers using Shade Guide VITAPAN classical. The research showed that strawberry concentration of 100% have an average difference in the color of teeth is 6.40, lime concentration of 2.5 at 6.20 and carbamide peroxide 10% as the control group amounted to 3.20. Based on the Kruskal Wallis test there are differences in the average value of the observation color of the teeth was significant (P <0.05) and continued with different test further the Post Hoc Test Mann Whitney gained significant difference (p> 0.05) between treatment groups strawberry 100% with carbamide peroxide 10%, lime and 2.5% carbamide peroxide 10%, while among the treatment groups strawberries 100% with 2.5% lime fruit is not significantly different because it has a value of p> 0.05. Strawberry concentration of 100% more effective to whiten teeth than lime concentration of 2.5%, but there is no significant difference in teeth whitening, while lime is more effective than carbamide peroxide 10%. Keywords:Strawberry, lime, carbamide peroxide 10%, tooth whitening.

scholarly journals In Vitro Evaluation of the Efficacy of Different Over-the-Counter Products on Tooth Whitening

2015 ◽  
Vol 26 (4) ◽  
pp. 373-377 ◽  
Author(s):  
Muhammet Karadas ◽  
Zeynep Yesil Duymus
Keyword(s):  
Repeated Measures ◽  
Positive Control ◽  
Negative Control ◽  
Carbamide Peroxide ◽  
Tooth Whitening ◽  
Over The Counter ◽  
Color Changes ◽  
Significant Difference ◽  
Cielab System

<p>This <italic>in vitro</italic> study evaluated the whitening effect of four different over-the-counter products compared with a 10% carbamide peroxide (CP) bleaching gel. Specimens obtained from bovine incisors were stained in a tea solution and randomly divided into six groups according to the product used (n=10): CT: conventional toothpaste (negative control); CWT: Crest 3D White toothpaste; CWR: Crest 3D White mouth rinse; CWS: Crest 3D White strips; DW: Dazzling White (paint-on gel); and OP: Opalescence PF 10% CP (at-home bleaching gel, positive control). Over-the-counter products and 10% CP were used according to the manufacturer's recommendations. Color measurements were performed according to the CIELab system using spectrophotometer after staining, and after 4 and 8 weeks of the treatment procedure. The ∆E, ∆L, ∆a, and ∆b values were calculated. The data obtained were analyzed by repeated-measures ANOVA. Tukey's test for multiple comparisons was applied (p<0.05). All groups, except CWT, were effective in tooth whitening in comparison with conventional toothpaste. There was no significant difference in tooth whitening between CWT and CT (p=0.93). CWS, DW and OP groups presented significantly higher color changes than the CWR group. DW and CWS showed similar tooth whitening to OP.</p>

Analysis of Photoreflectance and Microhardness of the Enamel in Primary Teeth Submitted to Different Bleaching Agents

2007 ◽  
Vol 32 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Sabrina Fróes Faria Campos ◽  
Ilene Cristine Rosia César ◽  
Egberto Munin ◽  
Priscila Christiane Suzy Liporoni ◽  
Marcos Augusto do Rego
Keyword(s):  
Primary Teeth ◽  
Point Of View ◽  
Control Group ◽  
Sodium Perborate ◽  
Carbamide Peroxide ◽  
Color Changes ◽  
Significant Difference ◽  
Student’S T ◽  
Bleaching Treatment

Treatment of darkened teeth in children is of great importance from an esthetic-functional point of view and for the psychoemotional development of the child. The objective of the present study was to determine the in vitro efficacy of three bleaching agents for whitening of artificially stained primary teeth. Fifty anterior primary teeth were artificially stained and then divided into three experimental groups (n = 15) submitted to bleaching treatment with 35% hydrogen peroxide gel, 35% carbamide peroxide gel, and 35% carbamide peroxide gel mixed with sodium perborate powder. The control group (n = 5) was not submitted to any bleaching treatment. Color changes were evaluated with a reflectance spectrophotometer and possible alterations in the enamel surface after bleaching were measured by Vickers microhardness testing. The data were assessed using the Student's t test. The results confirmed the bleaching action of the three agents tested. No significant difference in mean microhardness was observed between the three bleaching agents when compared to the control group.

scholarly journals Effect of a carbamide peroxide bleaching gel containing calcium or fluoride on human enamel surface microhardness

2005 ◽  
Vol 16 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Rogério de Oliveira ◽  
Adriana Franco Paes Leme ◽  
Marcelo Giannini
Keyword(s):  
Artificial Saliva ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Surface Microhardness ◽  
Treatment Groups ◽  
Peroxide Bleaching ◽  
Human Enamel

This in vitro study evaluated the surface microhardness of human enamel submitted to bleaching with 10% carbamide peroxide (CP) containing calcium or fluoride. Ninety-eight dental blocks (5 x 5 mm²) with polished enamel surfaces were randomly assigned to 7 treatment groups (n=14), as follows: without bleaching and storage in artificial saliva (control); 10% CP; 10% CP + 0.05% calcium; 10% CP + 0.1% calcium; 10% CP + 0.2% calcium; 10% CP + 0.2% fluoride; and 10% CP + 0.5% fluoride. During 14 days, enamel surfaces were daily exposed to a 6-h bleaching regimen followed by storage in artificial saliva. Surface microhardness was measured before (baseline), during (7th day), immediately after bleaching (14th day) and 1 week post bleaching. Data were analyzed by two-way ANOVA and Tukey's test (p<0.05). All treatments reduced SM significantly during the bleaching cycle (7th day), immediately after bleaching (14th day) and 1 week post bleaching, compared to baseline and to the unbleached control group. In conclusion, in spite of the addition of calcium and fluoride, all bleaching treatments affected the enamel surface microhardness.

scholarly journals Effects of In-Office and Home Bleaching Gels on the Surface Mercury Levels of Dental Amalgam

2010 ◽  
Vol 04 (01) ◽  
pp. 023-027
Author(s):  
Parnian Alizadeh Oskoee ◽  
Mahdi Abed Kahnamoui ◽  
Siavash Savadi Oskoee ◽  
Firooz Zadfattah ◽  
Fatemeh Pournaghi-Azar
Keyword(s):  
Dental Amalgam ◽  
Control Group ◽  
Carbamide Peroxide ◽  
Water Control ◽  
Treatment Groups ◽  
Post Hoc ◽  
Bleaching Gels ◽  
One Way Anova ◽  
Electron Microscope Data

ABSTRACTObjectives: The aim of this study was to evaluate the effect of different in-office and home bleaching gels on the surface mercury levels of dental amalgam.Methods: Sixty disk-shaped amalgam specimens (GS-80, SDI- Australia) were prepared and randomly divided into the following treatment groups: 1. Distilled water (control); 2. 15% home-bleach carbamide peroxide (Opalescence PF, Ultra dent, USA) applied for 6 h/day for 3 weeks; and 3. 35% in-office bleach carbamide peroxide (Opalescence Quick, Ultradent) applied for 30 min/week for 3 weeks. Levels of mercury were measured as weight percentages using an energy dispersive x-ray micro-analyzer detector connected to an electron microscope. Data was analyzed using one-way ANOVA and a post hoc Tukey tests (P<.05).Results: There were no significant differences between the surface mercury levels measured following treatment with the tested home-bleach and in-office bleach products (P=0.71). However, both materials yielded significantly more mercury levels than that of the control group (P<.001).Conclusions: The tested bleaching products significantly elevated the surface mercury levels of amalgam in vitro. (Eur J Dent 2010;4:23-27)

scholarly journals Effect of exposure frequencies of pear juice on tooth whitening

2013 ◽  
Vol 25 (1) ◽  
Author(s):  
Gobalakrishna Munusamy ◽  
Endang Sukartini ◽  
Ayu Trisna Hayati
Keyword(s):  
Hydrogen Peroxide ◽  
Research Result ◽  
Control Group ◽  
Tooth Whitening ◽  
Whitening Effect ◽  
Significant Difference ◽  
Pear Juice ◽  
The Difference ◽  
Tooth Colour

Introduction: Tooth whitening (Bleaching) is a method to restore discoloured teeth to the original tooth colour with a chemical process. The materials used in the bleaching process are hydrogen peroxide, sodium perborates and carbamide peroxide. Pear fruit consists of hydrogen peroxide which is potentially an oxidizing compound that produces free radicals that react with unsaturated bonding teeth staining substances. This study aims to analyzed tooth whitening effect of exposure of pear juice with different frequencies. Methods: The type of research was true in vitro experiment where samples of 32 maxillary premolar teeth had cut the root to CEJ. The specimens divided into four groups. The first group was soaked in pear juice two times, the second group three times, a third group four times, and the fourth was the control group using only mineral water. Tooth colour was measure using a Spectrophometer, conducted before and after individual soaking. Results: The research result is tested with ANOVA and Newman- Keuls Range Test showed a significant difference of tooth whitening degree between the frequency exposure pear juice after 2 times soaking, 3 times soaking and 4 times soaking. Conclusion: There are difference degree of whitening from the difference frequency exposure of pear juice and Pear juice exposure of 4 times gives higher whitening effect compared to frequency of 3 times, 2 times and control group which is soaked in water.

163 IN VITRO CULTURE OF PORCINE OOCYTE-GRANULOSA CELL COMPLEXES

2011 ◽  
Vol 23 (1) ◽  
pp. 184
Author(s):  
Y. F. Diao ◽  
R. X. Han ◽  
H. R. Kim ◽  
C. S. Park ◽  
D. I. Jin
Keyword(s):  
Survival Rate ◽  
Granulosa Cell ◽  
Granulosa Cells ◽  
Control Group ◽  
Oocyte Diameter ◽  
Cell Complexes ◽  
Treatment Groups ◽  
Significant Difference ◽  
Porcine Oocyte

The objective of this study was to investigate the effects of porcine follicular fluid (PFF) and insulin-like growth factor-1 (IGF-1) on the growth of porcine oocyte-granulosa cell complexes (OGC) in vitro, in an effort to improve meiotic and developmental competence in vitro. Porcine OGC were manually dissected from early antral follicles of diameter 400 to 700 μm, and intact oocytes with undamaged granulosa cells were selected for culture. Between 3 and 5 OGC were combined in 50-uL droplets and cultured for 12 days in M199 medium supplemented with PVP, oestradiol, FSH, transferrin, L-ascorbic acid, and insulin. The OGC were cultured at 38.5°C in a humidified atmosphere of 5% CO2 in air for 12 days, and the oocytes were matured for 44 h. Oocyte diameter, exclusive of the zona pellucida, was measured on day 0 and day 12 of culture. Control group was cultured in the absence of PFF or IGF-1. The experiment was divided into 2 parts. In part 1, treatment groups were cultured with 2.5, 5.0, or 7.5% (all v/v) PFF. Control OGC grew as spheres that were formed by granulosa cells. In treatment groups, the granulosa cells spread and grew on the bottom of dishes. When oocyte diameter was measured after 12 days of culture, no significant difference among groups was observed (104.07, 103.96, and 104.27 μm at the 3 PFF concentrations used; control: 104.03 μm). Similarly, the survival rate of oocytes did not differ significantly among groups. However, survival rate fell somewhat in the group treated with PFF (control: 65%; tests: 58.87, 58.33, and 50.83% at the 3 PFF concentrations used). The maturation rates of oocytes in the control was significantly higher than those of the treatment groups [25.83% (control) v. 14, 11.67, and 3.67% (the 3 treatment groups); P < 0.05]. Thus, the first conclusion is that supplementation of culture medium with PFF did not enhance the development of porcine OGC in vitro. In part 2 of the experiment, treatment groups were cultured with 10, 50, or 100 ng mL–1 IGF-1. The percentages of OGC showing antrum formation were 80, 80, 100, and 100% in groups treated with 0, 10, 50, or 100 ng mL–1 IGF-1, respectively. Average oocyte diameter was 94.16 to 94.58 μm just after OGC collection. However, the average diameter of oocytes cultured for 12 days with 50 or 100 ng mL–1 IGF-1 was significantly higher than that of the control or 10 ng mL–1 groups [108.88 and 108.31 μm (50 and 100 ng mL–1 IGF-1) v. 105.98 μm and 106.67 μm (0 and 10 ng mL–1 IGF-1); P < 0.05]. The maturation rate of oocytes grown with 10 and 50 ng mL–1 IGF-1 was higher than those of the other 2 groups [30 and 40.6% (10 and 50 ng mL–1 IGF-1, respectively) v. 25 and 26% (0 and 100 ng mL–1 IGF-1, respectively); P < 0.05]. Thus, the second conclusion is that 50 ng mL–1 IGF-1 improves the growth and maturation of porcine oocyte-granulosa cell complexes in vitro.

scholarly journals Employment of Reservoirs in At-Home Whitening Trays: Efficacy and Efficiency in Tooth Whitening

2015 ◽  
Vol 16 (5) ◽  
pp. 383-388 ◽  
Author(s):  
Fang Qian ◽  
Serena Geisinger ◽  
So Ran Kwon
Keyword(s):  
Color Change ◽  
Carbamide Peroxide ◽  
Night Time ◽  
Tooth Whitening ◽  
Treatment Groups ◽  
Significant Difference ◽  
Post Hoc ◽  
Artificial Teeth ◽  
One Way Anova ◽  
At Home

ABSTRACT Aim This study aimed to determine the efficacy of trays made with and without reservoirs, in conjunction with time and cost evaluations, by measuring color change with home whitening procedures. Materials and methods Extracted human maxillary teeth (central incisors n = 20; canines n = 20; molars n = 20) and 60 artificial teeth (lateral n = 20; premolar n = 40) were mounted into ten typodonts. Tray fabrication was completed such that a blockout resin reservoir was placed on half of the buccal surface of the tray, while the other half remained without a reservoir. Whitening with custom fabricated trays was performed based on two different whitening regimens, where each regimen was assigned to five typodonts: Night-time: Opalescence PF 10% carbamide peroxide for 8 hours daily and Day-time: Philips DayWhite 9.5% hydrogen peroxide for 30 minutes, twice daily. Both systems were applied for 1 week. To evaluate tooth shade, the VITA Easyshade® Advance 4.0 spectrophotometer was used. Color measurements were obtained at baseline (T1), 1-day post-whitening (T2), and 1 month post-whitening (T3). One-way ANOVA, followed by post-hoc Tukey's HSD test, was used to detect significant difference in the overall color change (ΔE*) among the four groups at T2 and T3. Additionally, paired-sample t-test was used to assess difference in ΔE* between T2 and T3 treatment within each of four techniques of tray fabrication. Results No significant difference in ΔE* was found among the four groups at T2 and T3 (p > 0.05 in each instance). There were significant differences in mean ΔE* between T2 and T3 treatment for the day white treatment groups without reservoir (6.96 vs 10.19 respectively; p = 0.0026) and with reservoirs (6.23 vs 9.79 respectively; p = 0.0031). Conclusion The use of reservoirs does not have a significant effect on whitening efficacy, regardless of type of whitening material and regimen. Clinical significance The use of custom fabricated trays with or without reservoirs were equally effective in whitening teeth. How to cite this article Geisinger S, Kwon SR, Qian F. Employment of Reservoirs in At-Home Whitening Trays: Efficacy and Efficiency in Tooth Whitening. J Contemp Dent Pract 2015;16(5):383-388.

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO

2021 ◽  
Vol 17 (2) ◽  
pp. 97-102
Author(s):  
Dwis Syahrul ◽  
◽  
Dwis Syahriel ◽  
Keyword(s):  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Culture Medium ◽  
Ocimum Basilicum ◽  
Optimal Concentration ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Inhibitory Power

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

scholarly journals Effect of Remineralizing Agents on Tooth Color After Home Bleaching

2019 ◽  
Author(s):  
Mohammadreza Malekipour ◽  
Zahra Norouzi ◽  
Sahar Shahlaei
Keyword(s):  
Surface Treatment ◽  
In Vitro Study ◽  
Control Group ◽  
Tooth Color ◽  
Treatment Groups ◽  
Tooth Discoloration ◽  
Significant Difference ◽  
The Mean ◽  
Post Hoc

Objectives: The purpose was to investigate the effect of casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste, Remin Pro paste, and 0.05% sodium fluoride (NaF) mouthwash on teeth discoloration after home bleaching. Materials and Methods: In this in-vitro study, 48 intact and unstained human incisors were divided into four groups (n=12): control (Group 1), CPP-ACPF paste (Group 2), Remin Pro paste (Group 3), and 0.05% NaF mouthwash (Group 4). The specimens were bleached using 20% carbamide peroxide gel for 21 days and were subjected to pH cycling and surface treatment with remineralizing agents for one month. The color of the specimens was determined according to the CIELAB color space at baseline, after bleaching, and after surface treatment. Data were analyzed using one-way analysis of variance (ANOVA), post-hoc least significant difference (LSD) test, and one-sample t-test (P=0.05). Results: ANOVA showed a significant difference in the mean ΔE of the four groups after surface treatment (P<0.05). Post-hoc LSD test showed a significant difference between the mean ΔE of the control group and those of the treatment groups (P<0.05) with no significant difference between the treatment groups, except for Remin Pro. The mean ΔE and ΔL of the Remin Pro group showed less change than those of the CPP-ACPF and NaF groups, indicating that Remin Pro has the lowest potential for post-bleaching tooth discoloration. Conclusion: CPP-ACPF paste, Remin Pro paste, and 0.05% NaF mouthwash cause noticeable teeth discoloration immediately after bleaching. Remin Pro has less effect on tooth color than the other two products.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

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