scholarly journals Perbedaan Efek Antibakteri Kapsul Minyak Bawang Putih (Garlic Oil) dan Kapsul Bubuk Bawang Putih (Garlic Powder) Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli Secara In Vitro

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Ismail Fajri ◽  
Erly Erly ◽  
Elly Usman
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Alternative Therapy ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Garlic Powder ◽  
Garlic Oil ◽  
Bacterial Effect

Abstrak   Bawang putih  telah diekstrak berupa kapsul garlic oil dan kapsul garlic powder sehingga dapat digunakan sebagai alternatif terapi dalam pengobatan penyakit akibat infeksi bakteri. Kandungan kapsul bawang putih yang memiliki efek anti bakteri adalah allicin. Bawang putih mempunyai efek anti mikroba dalam melawan bakteri Gram positif dan Gram negatif, diantaranya Staphylococcus aureus dan Escherichia coli. Di pasaran terdapat dua jenis kapsul bawang putih, yaitu kapsul bubuk bawang putih dan kapsul minyak bawang putih.  Tujuan penelitian ini adalah menentukan perbedaan efek antara antibakteri kapsul minyak bawang putih dan kapsul bubuk bawang putih terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.  Penelitian ini dilakukan di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas Padang dari Mei sampai Juni 2014. Jenis penelitian adalah eksperimental dengan menggunakan metode difusi cakram. Hasil penelitian didapatkan kapsul minyak bawang putih dan kapsul bubuk bawang putih tidak memiliki kemampuan untuk menghasilkan zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Walaupun tiga kali pengulangan,  tidak terdapat efek antibakteri pada kapsul minyak bawang putih dan kapsul bubuk bawang putih.Kesimpulan penelitian ini adalah kapsul minyak bawang putih dan kapsul bubuk bawang putih tidak memiliki efek antibakteri terhadap Staphylococcus aureus dan Escherichia coli.Kata kunci: minyak bawang putih, kapsul bubuk bawang putih, Staphylococcus aureus, Escherichia coli AbstractGarlic extract in garlic oil capsules and garlic powder capsules can be used as alternative therapy in the treatment of diseases caused by bacterial infection. The content of garlic capsules which have anti-bacterial effect is allicin. Garlic has anti-microbial effect against Gram positive and Gram negative, including Staphylococcus aureus and Escherichia coli. In the market there are two types of garlic capsules, the capsules of garlic powder and garlic oil capsules. The objective of this study was to determine the differences of antibacterial effect between garlic oil capsules and garlic powder capsules on the growth of Staphylococcus aureus and Escherichia coli. This research was done in the laboratory of Microbiology, Faculty of Medicine, Andalas University, Padang in Mei to Juny 2014. The type of research is experimental by using the disc diffusion method. The results of this study, garlic oil capsules and garlic powder capsules do not have the ability to produce a zone of inhibition against Staphylococcus aureus and Escherichia coli. Although 3 (three) time  repetitions, there were no antibacterial effect from garlic oil capsules and garlic powder capsules. The conclusion of this study is garlic oil capsules and garlic powder capsules do not have antibacterial effects against Staphylococcus aureus and Escherichia coli.         Keywords: garlic oil capsule, garlic powder capsule, Staphylococcus aureus, Escherichia coli

scholarly journals Perbandingan Efek Antibakteri Madu Asli Sikabu dengan Madu Lubuk Minturun terhadap Escherichia Coli dan Staphylococcus Aureus secara In Vitro

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Yugo Berri Putra Rio ◽  
Aziz Djamal ◽  
Asterina Asterina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Honey Bees ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Traditional Medicines ◽  
The Common ◽  
And Staphylococcus Aureus

AbstrakPendahuluan: Madu digunakan sebagai agen makanan dan obat tradisional, mengandung nektar atau gulaeksudat dari tanaman yang dikumpulkan oleh lebah madu serta merupakan salah satu obat tradisional yangdigunakan oleh masyarakat. Madu diketahui memiliki kemampuan sebagai efek antibakteri, seperti Escherichia colidan Stahpylococcus aureus. Kedua bakteri ini memiliki sifat yang berbeda tetapi memilki kesamaan dari penyakityang disebabkannya. Kualitas dan jenis madu juga sesuai dengan tempat dan lokasi lebah berkembang biak.Tujuan penelitian ini adalah untuk melihat perbandingan efek antibakteri madu asli Sikabu dan Lubuk Minturunterhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Metode Penelitian: Madu yang diuji untukpenelitian ini adalah madu yang berasal dari Sikabu dan Lubuk Minturun. Penelitian ini dilaksanakan pada bulanDesember 2011 sampai Mei 2012 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenispenelitian yang digunakan adalah eksperimental dengan menggunakan metode difusi (metode cakram) dananalitik dengan menghubungkan perbedaan yang dimiliki kedua madu sebagai efek antibakteri. Hasil Penelitian:ini menunjukkan bahwa madu asli Sikabu dan Lubuk Minturun tidak memiliki kemampuan efek antibakteri terhadapEscherichia coli, dan terdapat perbedaan efek antibakteri dari kedua madu terhadap Staphylococcus aureus.Kesimpulan: dari kedua jenis madu yang diteliti tidak ditemukan efek antibakteri terhadap Escherichia coli , namunmadu asli Sikabu memiliki efek antibakteri yang lebih baik dari pada madu Lubuk Minturun terhadapStaphylococcus aureusKata Kunci : Perbandingan Efek Antibakteri, Madu Asli Sikabu, Madu Lubuk Minturun. Escherichia coli,Staphylococcus aureusAbstractIntroduction: Honeyis used as an agent for food and traditional medicine, containing nectar or sugar exudates ofthe plant collected by honey bees, is one of the traditional medicines used by community. Honey is known to havethe ability as an antibacterial effect, such as Escherichia coli and Stahpylococcus aureus. Both of these bacteriahave different properties but have the common of the diseases it causes. The quality and type of honey is also inaccordance with the breeding places and their location. The purpose of this study was to see a comparison of theantibacterial effect of honey Sikabu and Lubuk Minturun against Escherichia coli and Staphylococcus aureus by invitro. Methods: Honey to be tested for this study were derived from Sikabu and Lubuk Minturun. This study wasconducted in December 2011 to May 2012 in the laboratory of Microbiology, Faculty of Medicine, AndalasUniversity. Type of research is experimental diffusion method (disc method) and analytical distinction owned bylinking the two of honey as an antibacterial effect. Results: These results indicate that the honey Sikabu and LubukMinturun have no antibacterial effect against Escherichia coli, both of honey have differences antibacterial effectagainst Staphylococcus aureus. Conclusion: Both types of honey from this study did not find an antibacterial effectagainst Escherichia coli, but honey Sikabu has better antibacterial against Staphylococcus aureus than honeyLubuk Minturun.Keywords: Comparisons Antibacterial Effects, Original Sikabu Honey, Honey Lubuk Minturun. Escherichia coli,Staphylococcus aureus

scholarly journals Antimicrobial Activity of Benzimidazoles Derivatives

2020 ◽  
Vol 4 (11) ◽  
pp. 23-27
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Diffusion Method ◽  
Pathogenic Microorganisms ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Electron Withdrawal ◽  
Microbial Action

The anti-microbial action of benzimidazole of ampicillin, was examined in Vitro beneath a specific conditions, utilizing the disc diffusion method, against different gram-positive and gram-negative pathogenic microorganisms such as Bacillus cereus, Staphylococcus aureus Pseudomonas aeruginosa and Escherichia Coli. An arrangement of these compounds were arranged and have appeared to hinder phthogenic development, the range of the zone of restraint. The zone of Obstacles of compounds founds from 7 mm2 to 46 mm2 Among the synthesized compounds in common. 6-Methoxy-N-phenyl- 1H-benzo [d]imidazol-2-amine(1c, zone of inhibition 9 mm2 at 40 µg/ml against Escherichia coli ) and 6-Chloro-N-phenyl-1H-benzo [d]imidazol-2-amine (1d, zone of inhibition 18 mm2 at 40 µg/ml against Bacillus cereus) have appeared great movement. While 1j, zone of inhibition 20 mm2 at 40 µg/ml), and (1i, zone of inhibition 20 mm2 at 40 µg/ml) were found to be displayed directly to great action against Bacillus cereus and Escherichia coli. 1p did not appear any movement against Staphylococcus aureus .1e did not appear any movement against Escherichia Coli and Staphylococcus aureus.The results have shown clearly that the contribution of el ectron -donation and electron- withdrawal to the aromatic ring i ncreases antibacterial activity. Target benzimidazolessamples sho wed antibacterial & reference antibiotic ampicillin in vitro.

scholarly journals Perbedaan Daya Hambat Bakteri dari Propolis Cair yang Ada di Pasaran Terhadap Escherichia Coli dan Staphylococcus Aureus Secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Bangun Azhari Yusuf ◽  
Aziz Djamal ◽  
Asterina Asterina
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Market Research ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Breeding Location ◽  
Main Component ◽  
And Staphylococcus Aureus ◽  
Better Than

Abstrak Propolis adalah zat yang digunakan lebah melindungi sarangnya dari berbagai ancaman. Komponen utama propolis adalah resin yang dikumpulkan lebah dan dicampur dengan air liurnya. Manusia juga menggunakan propolis sebagai obat berbagai penyakit, seperti infeksi bakteri. Propolis memiliki kemampuan antibakteri terhadap Escherichiacoli dan Staphylococcus aureus. Kualitas dan jenis propolis sesuai dengan komposisi kimia, iklim dan tempat berkembang biak. Tujuan penelitian ini adalah melihat perbedaan daya hambat bakteri Propolis cair 1 dan Propolis cair 2 yang ada di pasaran terhadap Escherichia coli dan Staphylococcus aureus secara in vitro. Propolis yang akan diuji adalah propolis cair yang ada di pasaran dipilih dua secara acak. Penelitian dilaksanakan pada bulan Januari 2013 di laboratorium Mikrobiologi Fakultas Kedokteran Universitas Andalas. Jenis penelitian eksperimentalmenggunakan metode difusi (metode cakram) dan analitik dengan menghubungkan perbedaan kedua propolis sebagai antibakteri. Hasil penelitian ini menunjukkan propolis cair 1 dan propolis cair 2 yang ada di pasaran tidak memiliki efek antibakteri terhadap Escherichia coli dan terdapat perbedaan daya hambat bakteri dari kedua propolisterhadap Staphylococcus aureus. Propolis cair 2 (diklaim berasal dari Selandia baru) memiliki daya hambat bakteri yang lebih baik daripada propolis cair 1 (diklaim berasal dari Malaysia).Kata kunci: propolis cair, perbedaan daya hambat bakteri, Escherichia coli, Staphylococcus aureusAbstract Propolis is substance which use to protect bee hive from various threat. The main component of propolis is resin which is collected and mixed with saliva. The human also use propolis as a treatment against various illness such as bacterial infection. Propolis has an antibacterial effect to Escherichia coli and Stahpylococcus aureus. The quality and type of propolis is also in accordance with chemical composition, climate and their breeding location. The objective of this study was to find out the differentiation of the antibacterial effect of Propolis 1 and Propolis 2 in the market to Escherichia coli and Staphylococcus aureus by in vitro. Propolis to be tested for this study is random liquid propolis in the market. Research was conducted in January 2013 in the laboratory of Microbiology Faculty of Medicine, University of Andalas. This type of study was the experimental diffusion method (disc method) and the analytic distinction owned by linking two of propolis as an antibacterial effect. These results indicate that propolis 1 and propolis 2 have no ability antibacterial effect to Escherichia coli, and both of propolis have differences antibacterial effect to Staphylococcus aureus. Propolis 2 (from New Zealand claimed) has antibacterial effect better than propolis 1 (from Malaysia claimed).Keywords: propolis, differentiation of the antibacterial effect, Escherichia coli, Staphylococcus aureus

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals In vitro and in vivo evaluation of antibacterial activity of Bridelia ferruginea extracts on some clinical isolates

2018 ◽  
Vol 7 (4) ◽  
pp. 392-398
Author(s):  
B.T Yunana ◽  
◽  
B. B Bukar ◽  
J. C Aguiyi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Root Bark ◽  
Zone Of Inhibition ◽  
Bridelia Ferruginea

The ethanol extracts of root, bark and leaf of Bridelia ferruginea was investigated for antibacterial activity against clinical isolate of Staphylococcus aureus and Escherichia coli. The extracts had significant antibacterial activity in vitro at concentration of 25 mg/ml, 50 mg/ml, 100 mg/ml and 200 mg/ml and in vivo at dose of 50 mg/kg and 100 mg/kg. The root extract in vitro had the highest zone of inhibition, followed by the bark extract for both Staphylococcus aureus and Escherichia coli. The concentration of 200 mg/ml had the highest zone of inhibition in vitro. The minimum inhibitory concentration (MIC) showed a decreasing inhibitory effect of the plant extracts for both Staphylococcus aureus and Escherichia coli as the concentration decreases with root having 3.125 mg/ml, bark having 6.25 mg/ml and leaf having 25 mg/ml for Staphylococcus aureus and Escherichia coli. Likewise, the minimum bactericidal concentration (MBC) showed decreasing bactericide effects with decrease concentration with root having 12.5 mg/ml, bark having 12.5 mg/ml and leaf having 25 mg/ml for Escherichia coli while root had 6.25mg/ml, bark had 12.5mg/ml and leaf had 25mg/ml for Staphylococcus aureus. The in vivo investigation showed that the root and bark extract exhibited antibacterial activity on both Staphylococcus aureus and Escherichia coli at doses of 100mg/kg and 50mg/kg; the root extract had higher activity than the bark and root/bark combined. The dose of 100 mg/kg had the highest colonies reduction for Staphylococcus aureus and Escherichia coli in vivo. Preliminary phytochemical screening of root, bark and leaves of Bridelia ferruginea revealed the presence of tannins, flavonoids, carbohydrates, cardiac glycoside (root, bark and leaves), saponins (root and bark). The presence of tannins, saponins, flavonoid, cardiac glycoside and carbohydrate in the bark and root extracts of the plant indicates that the bark and root extracts were pharmacological importance

scholarly journals Antibacterial effect of Dimethyl sulfoxide extract of Aloe vera (Aloe barbadensis) leaf gel against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae

Mediscope ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 67-74 ◽  
Author(s):  
Syed Didarul Haque ◽  
Abu Md Mayeenuddin Al Amin ◽  
Baishakhi Islam ◽  
Nazia Nazneen ◽  
Syeda Noorjahan Karim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Dimethyl Sulfoxide ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Biologically Active ◽  
Diffusion Method ◽  
E Coli

An exploratory study based on laboratory experiment was carried out to determine the antibacterial effect of Dimethyl sulfoxide (DMSO) extract of Aloe vera leaf gel (DAE) against standard strains of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae in the Department of Pharmacology & Therapeutics in collaboration with the Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh. DMSO extract was used in five different concentrations (100, 200, 300, 400 and 500 μg/ml). Dose dependent inhibitory effect was seen against the test organisms using disc diffusion method. Zone of inhibition (ZOI) were 8 mm, 13 mm, 15 mm, 16 mm and 21 mm against S. aureus; 0 mm, 8 mm, 13 mm, 15 mm and 18 mm against P. aeruginosa; 8 mm, 11 mm, 13 mm, 16 mm and 20 mm against E. coli; 0 mm, 9 mm, 12 mm, 14 mm and 18 mm against K. pneumoniae at 100, 200, 300, 400 and 500 μg/ml respectively. The minimum inhibitory concentration (MIC) was assessed by broth dilution technique. The MICs of DAE for S. aureus, P. aeruginosa, E. coli and K. pneumoniae were 300 μg/ml, 400 μg/ml, 400 μg/ml and 450 μg/ml respectively. From the study it was observed that DMSO extract of Aloe vera leaf gel possesses antibacterial effect against the test pathogens. The findings highlight the need for further extensive study to detect and isolate the biologically active ingredients present in the Aloe vera leaves which are responsible for antibacterial effect. Hopefully, that would lead to the discovery of new and more potent antimicrobial agents originated from Aloe vera. Mediscope Vol. 7, No. 2: July 2020, Page 67-74

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals Test the effectiveness of antibacterial effect of the skin of the pomegranate fruit (Punica granatum) extract against the growth of Escherichia coli in vitro

2020 ◽  
Vol 7 (5) ◽  
pp. 1680
Author(s):  
Niken . ◽  
Eliza Arman ◽  
Rahmi Novita Yusuf ◽  
Honesty Diana Morika
Keyword(s):  
Escherichia Coli ◽  
Bacterial Resistance ◽  
Antibacterial Effect ◽  
Punica Granatum ◽  
Positive Control ◽  
Diffusion Method ◽  
Skin Extract ◽  
Design Study ◽  
Pomegranate Peel

Background: Pomegranate (Punica granatum) is one of the traditional medicines that contains antibacterial compounds that are effective against bacterial growth. Its chemical content includes saponins, tannins and flavonoids. Escherichia coli is a gram-negative bacterium which is a normal flora germ found in the human large intestine. These bacteria are pathogenic when they are outside the intestine and produce enterotoxins in epithelial cells which cause diarrhea. The purpose of this study was to determine the antibacterial effect of pomegranate skin extract on the growth of Escherichia coli.Methods: This study used an experimental design study with a complete randomized design study divided into 5 groups namely groups 1 (15 ug/ml), 2 (20 ug/ml), 3 (25 ug/ml), positive control (ciprofloxatin), negative control. Making pomegranate peel extract was done by maceration method then rotary, after that the effectiveness of pomegranate extract extracted by the diffusion method was tested using Anova one-way test.Results: The results showed that the extract of pomegranate peel showed that it was able to inhibit the growth of Escherichia coli with a ratio of constants (15 ug/ml, 20 ug/ml, 25 ug/ml with inhibition diameters of 6.7 mm, 6.7 mm, 6, 7 mm, while for positive control with ciprofloxatin showed bacterial resistance to antibiotics.Conclusions: Statistically, pomegranate skin extract has an antibacterial power which is meaningful with p 0.005. Pomegranate rind extract has antibacterial activity against Escherichia coli medium because inhibition zone is 5-10 mm.

scholarly journals ANTIBACTERIAL PHOTODYNAMIC THERAPEUTIC STUDIES OF METALLATED PORPHYRIN AGAINST CHRONIC WOUND COLONISING BACTERIAL ISOLATES

2021 ◽  
Vol 46 (2) ◽  
Author(s):  
O. B Daramola ◽  
A. A Olajide ◽  
N Torimiro ◽  
R. C George
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Chronic Wound ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
In Vitro Toxicity ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Bacterial Strains

Wound infections have become life threatening as a result of treatment failures caused by multi-drug resistant pathogens. The search for newer compounds potent against antibiotic resistant bacteria associated with wounds is crucial. Hence this study investigated the application of antibacterial photodynamic therapy using meso tetra-(4-phenyl) porphyrin (TPP), metallated with zinc, tin and silver (ZnTPP, SnTPP and AgTPP), meso tetra-(4-sulphonatephenyl) porphyrin (TPPS) and the corresponding metallo meso tetra-(4-sulphonatephenyl) porphyrin (MTPPS) as photosensitizers. The in-vitro toxicity and photo-toxicity properties on four chronic wound colonizing multi-drug resistant bacterial strains: Staphylococcus aureus, Klebsiella sp., Proteus sp., and Escherichia coli were assessed using agar well diffusion method. Photo-toxicity of the compounds was investigated using 100 Watt tungsten lamp. Inhibitory activity of porphyrins tested against these bacterial strains showed Staphylococcus aureus to have both lowest (11±0.0 mm) and highest (33±1.1 mm) susceptibility to SnTPPS and ZnTPPS respectively. The sequence of data also showed appreciable improvement in the antimicrobial activities of five metalloporphyrins (SnTPP, AgTPP, ZnTPPS, SnTPPS and AgTPPS) exposed to light rays than when tested against bacterial strains in dark condition. ZnTPPS exhibited the best activity with improved photo-toxic activities against all bacterial strains (Staphylococcus aureus 33±1.1 mm, Klebsiella sp. 32±0.7 mm, Proteus sp. 28±0.7 mm and Escherichia coli 30±1.4 mm) examined in this study.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

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