scholarly journals Effects of Organic Selenium on Laying Performance, Selenium Absorption and Utilization, Antioxidant Activity and Immunity in Laying Hens

2021 ◽  
Vol 6 (2) ◽  
pp. 336-344
Author(s):  
Weixian Zhang ◽  
◽  
Chunmei Pan ◽  
Yong Li ◽  
Li Zheng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Egg Production ◽  
Laying Hens ◽  
Control Treatment ◽  
Egg Mass ◽  
Organic Selenium ◽  
Inorganic Selenium ◽  
Laying Performance ◽  
Valuable Product

To study on influences of organic selenium on laying performance, Se absorption and utilization, immunity and antioxidant activity, 400（laying rates 85%）hens during the period of 43-week were randomly allocated to 1 of 4 homogeneous treatments： (control treatment，no added Se feed; Na selenite, 0.3 mg Se/kg; yeast selenium, 0.3 mg Se/kg and DL-methionine selenium 0.3 mg Se/kg). Every treatment had 10 replicates, every replicate had 5 hencoops and every hencoop had 2 hens. We hypothesized that organic selenium was better than inorganic selenium, and DL-selenium had advantages over them of yeast selenium in some ways for laying hens. The experiment lasted for 77 d, with the first 7 d for adaptation. Egg production, laying rates and dry matter intake were recorded every day. And Se contents of serum, whole eggs, heart and liver, blood antioxidant and immunity index were analyzed in the 78th d of the study. The results showed Se sources had no significant effects on laying performance and eggs qualities （ P ＞ 0.05 ）, except for the groups of organic selenium increased egg mass or had trends of increasing laying rates compared with the groups of control and inorganic selenium in the tenth week significantly respectively（P﹤0.05 or P=0.07, P=0.06 and P=0.08）. The groups of organic selenium had increased the contents of Se in serum, whole eggs, heart and liver very significantly（P﹤0.01）；and increased glutathione peroxidase activity and antioxidant capacity significantly（P﹤0.05）. At same time, the groups of organic selenium had increased significantly or had trends to increase the immunoglobulin G in serum（P ﹤0.05 or P=0.05）. In a word, adding organic selenium in diets could improve laying performance, strengthen Se absorption and utilizations, antioxidant capacity and immunity of laying hens to the extent in a longer experiment. And the effects of DL-selenium had advantages over them of yeast selenium in some ways. It implicated that DL-selenium was benefited to production of laying hens and selenium-enriched eggs. Key words： Selenium；Laying performance; Absorption and utilization；Antioxidant activity; Immunity Summary text: Selenium is an essential mineral not only for animal nutrition, but also for humans’ nutrition. Organic selenium had more advantages in improvement of laying performance, Se absorption and utilizations, antioxidant capacity and immunity of laying hens. Especially, DL-selenium was a valuable product, which was benefits to improve production of laying hens and develop functional food, selenium-enriched eggs.

Influence of peeling and inorganic calcium concentration on the utilisation of giant taro (Alocasia macrorrhiza) corm meal by laying hens

2019 ◽  
Vol 59 (8) ◽  
pp. 1553 ◽  
Author(s):  
S. S. Diarra ◽  
I. Wamekeni ◽  
A. Vunagilee ◽  
H. Lavaka ◽  
T. Finau
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Shape Index ◽  
Egg Mass ◽  
Feed Conversion ◽  
Shell Quality ◽  
Laying Performance ◽  
Calcium Source ◽  
Main Effects

The effects of peeling and limestone flour level on the utilisation of Alocasia macrorrhiza corm meal (ACM) by laying hens were investigated. Maize-based diets with two concentrations of limestone flour (40 and 60 g/kg) and diets containing 200 g peeled and unpeeled ACM meal/kg with the same levels of limestone flour were fed to five replicate pens containing 10 20-week-old Shaver brown pullets (1635 ± 148 g), each for 105 days. Results showed no interaction or main effects of ACM or limestone concentration on feed intake, egg weight, egg mass, feed conversion efficiency, egg shape index, surface area and Haugh unit (P > 0.05). Feeding whole ACM with 40 g limestone/kg diet reduced hen-day production and shell thickness (P < 0.05) but this was overcome by increasing limestone concentration to 60 g/kg diet. Peeled ACM with both concentrations of limestone flour maintained laying performance and egg quality. We concluded that feeding 200 g whole ACM depresses egg production and shell quality in laying hens, but increasing dietary limestone concentration overcomes this adverse effect. The concentration of limestone flour in the diet has no effect on laying performance. Higher inclusion levels of ACM, calcium source and level that will maintain laying performance and reduce feed cost need to be investigated.

Changes in performance, egg quality and blood parameters of laying hens fed selenium and oregano oil

2020 ◽  
Vol 60 (13) ◽  
pp. 1620
Author(s):  
H. Reshadi ◽  
M. Torki ◽  
H. Mohammadi
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Synergistic Effects ◽  
Blood Parameters ◽  
Egg Mass ◽  
Feed Conversion ◽  
Total Antioxidant

Context Organic and herbal additives in feed may lead to more healthy animal products. For this study, we hypothesised that an organic form of selenium and/or a herbal additive (oregano, Origanum vulgare L.) may improve performance of laying hens. Aims The study was designed to determine the effects of selenium source (SS, sodium selenite; or selenium yeast, SY), oregano essential oil (OEO) and a combination of Se and OEO on performance, egg quality and blood parameters of laying hens. Methods In total, 216 Lohmann LSL-Lite laying hens, 66 weeks of age, were assigned to feed on one of six diets: control group, receiving basal diet (BD); BD + 0.3 mg SS/kg; BD + 0.3 mg SY/kg; BD + 250 mg OEO/kg; BD + 250 mg OEO + 0.3 mg SS/kg; BD + 250 mg OEO + 0.3 mg SY/kg. For each treatment, there were six replicates of six hens each for 12 weeks. Key results Feed conversion ratio was higher (P < 0.05) with the OEO diet than in the control. The SY diet decreased egg production and the OEO diet decreased egg mass (P < 0.05), although this effect was reversed with the SY + OEO diet. This would illustrate a synergistic effect of OEO with SY. Highest and lowest yolk colour values were found with the SY and SS diets, respectively (P < 0.05). Serum uric acid was lower with diets containing Se than the control diet (P < 0.05). Glutathione peroxidase activity was highest with SY and SY + OEO diets and lowest with the OEO diet (P < 0.05). Birds fed the SS + OEO diet showed the highest total antioxidant capacity and those fed SY showed the lowest total antioxidant capacity (P < 0.05). Conclusions Based on the results of the current study, it can be concluded that SY diet have increasing effect on egg weight in laying hens. In addition, novel synergistic effects between OEO and SY diets on improving egg production, egg mass and feed conversion ratio have been found. In terms of oxidative status, the detected synergistic effects between OEO and SS diets on total antioxidant capacity and between OEO and SY diets on glutathione peroxidase (GPx) have not been reported before, that appreciate more clarifying investigations in future. Although, dietary supplemental SY individually and also with OEO increased GPx, adding SS to diet together with OEO showed more improving effect on GPx compared to the separate usage. Implications Dietary supplemental SY can improve egg weight in laying hens. In addition, adding SY to diet of laying hens individually and with OEO have beneficial effects on oxidative status of bird in terms of GPs activity, which in turn can ameliorate the unfavourable impressions of probable environmentally oxidative stress on productive performance.

scholarly journals Laying performance and eggshell quality in laying hens fed diets supplemented with prebiotics and organic acids

2010 ◽  
Vol 55 (No. 7) ◽  
pp. 294-306 ◽  
Author(s):  
S. Świątkiewicz ◽  
J. Koreleski ◽  
A. Arczewska
Keyword(s):  
Organic Acids ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Feed Additives ◽  
Egg Mass ◽  
Positive Effects ◽  
Eggshell Quality ◽  
Laying Performance ◽  
Calcium And Phosphorus

The aim of the study was to evaluate the effect of organic acids and the prebiotic fructans on egg production and eggshell quality when added to the layer diet with different levels of calcium and phosphorus. The experiment was carried out on 168 Bovans Brown hens, allocated to 14 groups of 12 replications. Each hen (replication) was kept in an individual cage 40 cm × 40 cm in size. A 2 × 7 factorial arrangement, with two dietary levels of calcium and phosphorus (normal – 3.70% Ca, 0.65% P, and reduced – 3.25% Ca, 0.60% P) and with diets supplemented by selected additives (none, 0.75% inulin, 0.75% oligofructose, 0.50% volatile fatty acids (VFA), 0.25% medium chain fatty acid (MCFA), 0.30% VFA + 0.20% MCFA, 0.75% inulin + 0.50% VFA) was used. The experiment was carried out over 34 weeks, from the age of 26 to 70 weeks. There were no statistically confirmed effects of the factors studied in this experiment on egg performance, i.e. laying rate, egg mass, feed intake and feed conversion. Reducing the dietary levels of Ca and P significantly decreased eggshell percent, thickness, density and breaking strength. The additives used had a considerable effect on eggshell quality at 46, 58 and 70 weeks of age, and these positive effects were most pronounced in the case of inulin and MCFA. There was no significant interaction between Ca and P dietary levels and the additives used. It was thus concluded that selected feed additives which lower the pH of the diet and intestinal content can beneficially influence eggshell quality in older high-producing laying hens.  

scholarly journals Performance of Laying Hens Fed Sorghum Based Diets Supplemented with Enzymes

2021 ◽  
Vol 43 (1) ◽  
pp. 224-232
Author(s):  
O. M. Agunbiade ◽  
P. A. Onimisi ◽  
J. J. Omage
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Quality Characteristics ◽  
Egg Laying ◽  
The Other ◽  
Control Treatment ◽  
Feed Cost ◽  
Laying Performance ◽  
Dietary Treatments

Sorghum is the primary alternative feedstuff for corn in some developed countries in the production of poultry. Thus, four hundred and fifty 32 weeks old Lohmann Brown laying hens were used in a study to evaluate the effect of replacing maize with sorghum with or without enzymes supplementation, on egg laying performance and egg quality characteristics of laying hens. Birds were distributed randomly into six dietary treatments with three replicates per treatment, each replicate had 25 birds with uniform initial group weights. The treatments included maize without enzymes (T1); sorghum without enzymes (T2); as controls respectively, while T3, T4, T5, and T6 contained sorghum based diets with phytase, protease, G2G, and a combination of protease and G2G respectively. Laying performance, cost of production, and egg quality characteristics were determined for the period of the trial, 33-56 weeks of age of the birds. All data obtained were subjected to analysis of variance, using the completely randomized design (CRD) and significant differences among treatment means were compared using the Tukey test. Significant (p<0.05) differences were observed among dietary treatments but with no defined trend observed for egg production traits. Daily feed intake was significantly (p<0.05) higher in maize control diet compared to the other treatments. Diets supplemented with phytase (T3), protease (14), and combination of protease and roxazyme G2G (T6) had significantly (p<0.05) better feed conversion ratio with T3 having the lowest value of 4.01 compared to the other treatments. For feed cost/dozen egg (N), Kg feed/dozen eggs, income above feed cost at N30 per egg, and income above control treatment; phytase (T3), protease (T4), roxazyme 626 (T5), and protease + roxacyme G2G (T6) diets in this study showed better performance than the control. Sorghum diet without enzyme had significantly (p<0.05) least performance for egg number. Hen housed egg production (HHP) and hen day egg production (HDP) compared to the other treatments while sorghum diet with phytase had the highest value (60.12g/day) for average egg weight compared to the other treatments. Egg quality characteristics showed the best performance for dietary treatments supplemented with phytase (T3) in all the other parameters considered, apart from the albumen height, yolk diameter, and yolk index. It was concluded therefore, that sorghum with phytase and protease supplementations can be used in layers' diets as a substitute for maize for optimum egg production.

scholarly journals High Temperature With Low Humidity or Vice Versa Does Not Affect Performance and Physiology of Laying Hens

2020 ◽  
Author(s):  
Da-Hye Kim ◽  
Yoo-Kyung Lee ◽  
Sang-Ho Kim ◽  
Kyung-Woo Lee
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Density Lipoprotein ◽  
Scientific Basis ◽  
Egg Mass ◽  
Thermal Treatments ◽  
Feed Conversion ◽  
Laying Performance

The present study investigated the effect of different ambient temperature and relative humidity (RH) but equal temperature-humidity index (THI) on laying performance, egg quality, heterophil to lymphocyte ratio (H/L ratio), corticosterone (CORT) concentration in blood, yolk and albumen, and plasma biochemical parameters in laying hens. One hundred and twenty commercial hens (Hy-Line Brown) aged 60 weeks were allocated into 2 environmental chambers. Laying hens were subjected to either one of two thermal treatments, i.e., 26&ordm;C and 70% RH (LH75) and 30&ordm;C and 30% RH (HL75) for 28 days. Both thermal treatments had equal THI being 75. Neither LH75 nor HL75 affected (P &gt; 0.05) laying performance including egg production, egg weight, egg mass, feed intake, and feed conversion ratio. Plasma biochemical parameters such as total cholesterol, high-density lipoprotein cholesterol, triglyceride, calcium, magnesium, and phosphorus was not altered (P &gt; 0.05) by thermal treatments. As to the stress indicators, both environment regimes failed (P &gt; 0.05) to affect blood H/L ratio and CORT levels in plasma, yolk and albumen although albumen CORT levels were elevated (P &lt; 0.05) in LH75 vs. HL75 at days 3, 7, and 28. In conclusion, our study suggests that laying hens performed and responded equally when they were exposed to equal THI environment conditioned from either 26&ordm;C and 70% RH or 30&ordm;C and 30% RH. The results of this study will be served as a scientific basis for management decisions and handling under thermally challenging conditions.

scholarly journals Effects of Selenium Conjugated to Insect Protein on Pharmacokinetics of Florfenicol and Enrofloxacin in Laying Hens

2021 ◽  
Vol 8 ◽  
Author(s):  
Kai Qiu ◽  
Uchechukwu Edna Obianwuna ◽  
Jing Wang ◽  
Hai-jun Zhang ◽  
Guang-hai Qi ◽  
...  
Keyword(s):  
Feed Intake ◽  
Egg Production ◽  
Laying Hens ◽  
Drug Effects ◽  
Control Group ◽  
Poultry Production ◽  
Blank Control Group ◽  
Organic Selenium ◽  
Laying Performance ◽  
Significant Difference

In the context of increasing awareness on the dietary supplementation of organic selenium in commercial poultry production and ensuring safe egg production, the present study investigated the effects of selenium on the pharmacokinetics of the therapeutic use of florfenicol and enrofloxacin from perspectives of laying performance, selenium deposition in eggs, and drug residue in plasma, organs, and eggs. A 2 × 3 factorial arrangement with two kinds of drugs (florfenicol vs. enrofloxacin, 200 mg/kg) and three levels of dietary organic selenium SCIP (selenium conjugated to insect protein) (0, 2, and 5 mg/kg) was designed together with a blank control group. Healthy Hy-Line Brown laying hens (n = 252, 40-week-old and 90.0 ± 1.7% of egg production rate) were randomly allocated into one of seven treatments with six replicates and six hens per replicate. The experiment lasted for 42 days and consisted of three periods (adjusted stage, depositional stage, and eliminating stage) of 14 days each. These stages entail feeding of the laying hens with basal diets, addition of drugs and selenium synchronously into the diets, drug withdrawal from diet, and supply of selenium uninterruptedly in the diet. Egg production and feed intake were recorded on daily and weekly bases, respectively. The selenium content in egg yolk, egg white, and whole eggs and the drug residues in eggs, plasma, liver, kidney, and breast muscle were determined on days 2, 3, 5, 6, 7, 9, 11, and 14 of the depositional and eliminating stages. There was no significant difference (p &gt; 0.05) in egg production among the dietary treatments, but feed intake decreased significantly (p &lt; 0.05) in the drug treatment group compared to other groups. Dietary organic selenium decreased the residue of drugs in tissues and eggs, while the metabolism and deposition of selenium in laying hens were suppressed due to drug effects. The results of the present study are of significance to enrich the knowledge of the pharmacokinetics of florfenicol and enrofloxacin in laying hens and ensure the quality of poultry products.

scholarly journals Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 87
Author(s):  
Yoo-Bhin Kim ◽  
Sang-Hyeok Lee ◽  
Da-Hye Kim ◽  
Hyun-Gwan Lee ◽  
Yongjun Choi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Capacity ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Egg Quality ◽  
Inorganic Sulfur ◽  
Positive Effects ◽  
Laying Performance ◽  
Cont Group

The present study was conducted to investigate the comparative effects of organic and inorganic forms of sulfur, methyl sulfonyl methane (MSM) and sodium sulfate (SS), on laying performance, egg quality, ileal morphology, ileal volatile fatty acids, and antioxidant and stress markers in various biological samples in aged laying hens. A total of 144, 73-week-old Lohman Brown-Lite laying hens were randomly assigned to one of three experimental diets: basal diet (CONT), CONT + 0.2% MSM (MSM), and CONT + 0.3% SS (SS). The trial lasted for 12 weeks. MSM and SS diets contained 0.07% of sulfur, either organic or inorganic. Dietary MSM did not affect egg production or feed conversion ratio at 12 weeks compared with the CONT group. Dietary sulfur did not affect egg quality except for the Haugh unit at 4 weeks, which was lowered (p < 0.05) in the SS group. Compared with the CONT group, a higher (p < 0.05) villus height to crypt depth ratio was observed in the SS group. Dietary sulfur did not affect the percentages of short-chain fatty acids in the ileum. Total antioxidant capacity of the liver increased (p < 0.05) in laying hens fed MSM- and SS-added diets compared with the CONT group. The MSM and SS groups were found to have lowered (p < 0.05) malondialdehyde (MDA) concentration in serum samples compared with CONT. Finally, dietary MSM had the lowest (p < 0.05) MDA concentrations in yolk samples. Taken together, our study showed that dietary organic and inorganic sulfur have positive effects on ileal morphology and antioxidant capacity in laying hens. However, SS-mediated inhibition in laying performance needs to be clarified.

scholarly journals The Impact of Temperature and Humidity on the Performance and Physiology of Laying Hens

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 56
Author(s):  
Da-Hye Kim ◽  
Yoo-Kyung Lee ◽  
Sang-Ho Kim ◽  
Kyung-Woo Lee
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Density Lipoprotein ◽  
Scientific Basis ◽  
Egg Mass ◽  
Feed Conversion ◽  
Laying Performance ◽  
The Impact

We investigated the effect of different ambient temperatures and relative humidity (RH) with the same temperature-humidity indices (THI) on laying performance, egg quality, heterophil to lymphocyte ratio (H/L ratio), corticosterone (CORT) concentration in blood, yolk, and albumen, and plasma biochemical parameters of laying hens. Commercial hens (Hy-Line Brown; n = 120), aged 60 weeks, were allocated to two environmental chambers. Laying hens were subjected to either one of two thermal treatments—26 °C and 70% RH (TLHH75) or 30 °C and 30% RH (THHL75) for 28 days—with the same THI of 75. Neither TLHH75 nor THHL75 affected laying performance, including egg production, egg weight, egg mass, feed intake, and feed conversion ratio (p > 0.05). Plasma biochemical parameters such as total cholesterol, high-density lipoprotein cholesterol, triglycerides, calcium, magnesium, and phosphorus were not altered by the environmental treatments (p > 0.05). As for stress indicators, both environmental regimes failed to affect blood H/L ratio and CORT levels in plasma, yolk, and albumen (p > 0.05), although albumen CORT levels were elevated (p < 0.05) in TLHH75 group at day 7. Hence, our study suggests that laying hens performed and responded similarly when exposed to either TLHH75 or THHL75 characterized by the same THI. These results can serve as a scientific basis for management decisions and handling laying hens under thermally challenging conditions.

scholarly journals Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

2021 ◽  
Author(s):  
Yoo Bhin Kim ◽  
Sang Hyeok Lee ◽  
Da-Hye Kim ◽  
Hyun-Gwan Lee ◽  
Yongjun Choi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Capacity ◽  
Egg Production ◽  
Volatile Fatty Acids ◽  
Laying Hens ◽  
Egg Quality ◽  
Inorganic Sulfur ◽  
Positive Effects ◽  
Laying Performance ◽  
Cont Group

The present study was conducted to investigate the comparative effects of organic and inorganic forms of sulfur, methyl sulfonyl methane (MSM) and sodium sulfate (SS), on laying performance, egg quality, ileal morphology, ileal volatile fatty acids, and antioxidant and stress markers in various biological samples in aged laying hens. A total of 144, 73-week-old Lohman Brown-Lite laying hens were randomly assigned to one of three experimental diets: basal diet (CONT), CONT + 0.2% MSM (MSM), and CONT + 0.3% SS (SS). The trial lasted for 12 weeks. MSM and SS groups contained 0.07% of sulfur, either organic or inorganic. Dietary MSM did not affect egg production and feed conversion ratio at 12 weeks compared with the CONT group. Dietary sulfur did not affect egg quality except for Haugh unit at 4 weeks which was lowered (P &amp;lt; 0.05) in the SS group. Compared with the CONT group, higher (P &amp;lt; 0.05) villus height and crypt depth ratio was observed in the SS group. None of dietary sulfur affected the percentages of short-chain fatty acids in the ileum. Total antioxidant capacity of liver increased (P &amp;lt; 0.05) in laying hens fed MSM- and SS-added diets compared with the CONT group. The MSM and SS groups lowered (P &amp;lt; 0.05) malondialdehyde (MDA) concentration in serum samples compared with the CONT. Finally, dietary MSM had the lowest (P &amp;lt; 0.05) MDA concentrations in yolk samples. Taken together, our study showed that dietary organic and inorganic sulfur have positive effects on ileal morphology and antioxidant capacity in laying hens. However, SS-mediated inhibition in laying performance needs to be clarified.

scholarly journals Effect of Dietary Supplementation of Fermented Pine Needle Extract on Productive Performance, Egg Quality, and Serum Lipid Parameters in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1475
Author(s):  
Damini Kothari ◽  
Jong-Seok Oh ◽  
Ju-Hee Kim ◽  
Woo-Do Lee ◽  
Soo-Ki Kim
Keyword(s):  
Serum Lipid ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Egg Yolk ◽  
Egg Mass ◽  
Productive Performance ◽  
Laying Performance

This study aimed to investigate the supplemental effects of fermented pine (Pinus densiflora) needle extract (FPNE) in laying hen diets on productive performance, egg quality, and serum lipid metabolites. A total of 108 40-week-old Hy-line brown laying hens were randomly assigned to one of the three dietary treatment groups: (1) basal diet + 0 mL FPNE/kg diet (CON), (2) basal diet + 2.5 mL FPNE/kg diet (T1), or (3) basal diet + 5 mL FPNE/kg diet (T2) for 6 weeks. Each group consisted of four replicates of nine hens each. Feed and water provided ad libitum. Results showed that dietary supplementation of FPNE increased egg production percentage (linear, p < 0.01 and quadratic, p < 0.05), egg mass (linear, p < 0.05), and feed intake (linear, p < 0.05) during the entire experimental period. In addition, dietary inclusion of FPNE significantly increased the eggshell color (linear, p < 0.01), egg yolk color (quadratic, p < 0.01), and eggshell breaking strength (linear, p < 0.05 and quadratic, p < 0.05) while the Haugh unit decreased (quadratic, p < 0.05). However, serum lipid profile did not differ among the dietary treatments (p > 0.05). Notably, antioxidant activity of egg yolk was improved by significantly decreasing the malondialdehyde content in egg yolks after 6 weeks of storage (linear, p < 0.001 and quadratic, p < 0.05). In summary, dietary inclusion of FPNE could improve laying performance and the antioxidant capacity of eggs.

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