0946 Effect of supplementing milk during first 4 d postweaning on growth performance, energy digestibility, gut morphology, and severity of diarrhea for nursery pigs in a commercial farm

Fumonisin contamination in corn is an emerging issue in animal feed production. Fumonisin disrupts the metabolism of sphingolipids and reduces growth performance. This experiment was conducted to determine the effect of feeding fumonisin-contaminated corn on growth performance and sphinganine (SA) to sphingosine (SO) ratios of 9 to 28 kg pigs. A total of 350 pigs, were used with 5 pigs/pen and 14 pens/treatment. Dietary treatments contained fumonisin-contaminated corn (50 mg/kg of fumonisin B1 + B2) blended with low fumonisin corn (10 mg/kg of fumonisin B1 + B2) to provide dietary fumonisin concentrations of 7.2, 14.7, 21.9, 32.7, and 35.1 mg/kg. From day 0 to 28, increasing fumonisin concentration decreased (linear, p < 0.001) average daily gain, average daily feed intake (linear, p = 0.055), and gain:feed ratio (linear, p = 0.016). Although these response criteria tested linear, the greatest reduction in performance was in pigs fed with 32.7 and 35.1 mg/kg of fumonisin (B1 + B2). Increasing fumonisin concentration increased the serum SA:SO ratio (linear, p < 0.001) on day 14 and 28. In summary, for 9 to 28 kg nursery pigs, increasing fumonisin linearly decreased average daily gain and gain:feed ratio. However, despite the linear response, diets containing up to 21.9 mg/kg of fumonisin did not have as dramatic a decrease in growth performance as those fed more than 32.7 mg/kg. Further research is warranted to determine the effect of fumonisin concentrations between 21.9 and 32.7 mg/kg.

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PSV-8 Glucocorticoid receptor agonist (GRA): what is the best delivery method to newly weaned pigs?

Journal of Animal Science ◽

10.1093/jas/skaa054.279 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 158-159

Author(s):

Hailey Wooten ◽

Hwanhee Kim ◽

Amanda Rakhshandeh ◽

Anoosh Rakhshandeh

Keyword(s):

Growth Performance ◽

Performance Outcomes ◽

Nutrient Digestibility ◽

Control Group ◽

Free Access ◽

Weaned Pigs ◽

Delivery Methods ◽

Nursery Pigs ◽

Intestinal Integrity ◽

Randomized Design

Abstract We previously found that i.m. injection of GRA, similar to in-feed antibiotics, improves measures of intestinal integrity, nutrient digestibility, and overall growth performance in nursery pigs. In addition, we have demonstrated that the mitigating effects of GRA on the stress of weaning is predominantly done by subsiding the inflammatory response that often accompanies early weaning. The objective of the current study was to explore the most practical methods for delivering GRA to newly weaned pigs. One-hundred and sixty-seven PIC pigs were weaned at 25 ±1.0 days of age (BW 7 ±1.2 kg), and assigned to fourteen treatments (3-4 pigs /pen; 3 pens/treatment). Seven treatments were tested within two sexes (gilts vs. barrows). The treatments were: 1) i.m. injection of GRA (two injections, 0.2 mg/kg BW at -1 and 3 d post-weaning), 2) two levels of in-water GRA (0.8 and 1.6 ppm), 3) in-feed GRA (2.5 and 5.0 ppm), 4) in-feed antibiotic (ANT, 100 ppm tylosin), and 5) control. Dexamethasone was used as GRA. Pigs had free access to in-feed or in-water GRA or ANT during the 1st-week post-weaning. Parameters of growth performance were measured weekly until the end of the nursery phase. A completely randomized design and repeated measurement ANOVA (PROC MIXED) in SAS were used for statistical analyses. In-water, in-feed, and i.m. GRA treatments resulted in growth performance outcomes comparable to that of in-feed ANT. Relative to CON pigs, both ANT and GRA improved ADG and G: F during the nursery phase (P < 0.05). Among the delivery methods tested, 2.5 ppm of in-feed GRA resulted in superior ADG (GRA vs. CON; 0.29 vs. 0.20 kg/d) and G: F (0.63 vs. 0.40 ± 0.05), compared to the control group (P < 0.02). Collectively, these results suggested that in-feed treatment is the best method for delivering GRA to newly weaned pigs.

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263 Effects of soybean meal level on growth performance of 11- to 25-kg nursery pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.154 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 87-88

Author(s):

Henrique S Cemin ◽

Mike D Tokach ◽

Steve S Dritz ◽

Jason C Woodworth ◽

Joel M DeRouchey ◽

...

Keyword(s):

Mortality Rate ◽

Growth Performance ◽

Soybean Meal ◽

Random Effect ◽

Fixed Effect ◽

Nursery Pigs ◽

Energy Value ◽

Caloric Efficiency ◽

Main Effects

Abstract Four experiments were conducted to determine the effects of increasing soybean meal (SBM) in diets with or without 25% DDGS on growth performance of nursery pigs. Treatments were arranged in a 2 × 3 factorial with main effects of SBM (27.5, 32.5, or 37.5%) and DDGS (0 or 25%). A total of 296, 2,502, 4,118, and 711 pigs, initially 10.6, 11.7, 12.5, and 12.3 kg, were used and there were 10, 16, 13, and 12 replicates per treatment in Exp. 1, 2, 3, and 4, respectively. Experimental diets were fed for 21 d. Pigs were weighed and feed disappearance measured to calculate ADG, ADFI, G:F, and caloric efficiency (CE). Data were analyzed using the GLIMMIX procedure of SAS with block as random effect and treatment as fixed effect. The average cull rate was 0.7, 0.5, 0.2, and 0% and the mortality rate was 0.7, 0.3, 0.4, and 0% in Exp. 1 to 4, respectively. There were interactions (P ≤ 0.039) between SBM and DDGS for G:F and CE in Exp. 2 and for ADG and ADFI in Exp. 3. These were mostly driven by increasing SBM negatively affecting performance in a greater magnitude when diets contained DDGS compared to diets without DDGS. The main effects of DDGS and SBM were more consistent across experiments. Pigs fed diets with 25% DDGS had decreased (P ≤ 0.001) ADG and ADFI in all experiments as well as poorer (P ≤ 0.028) G:F and CE except for Exp. 3. Feeding increasing amounts of SBM generally did not result in any major impact in ADG, but consistently improved (linear, P ≤ 0.078) G:F and CE across experiments. The mechanism for this response is unclear but could be driven by intrinsic components of SBM or underestimating the energy value of SBM.

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PSVI-1 Dietary protease supplementation on growth performance and post-weaning diarrhea of nursery pigs

Journal of Animal Science ◽

10.1093/jas/skaa054.337 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 191-192

Author(s):

Jorge Y Perez-Palencia ◽

David A Clizer ◽

Christian Ramirez-Camba ◽

Ryan S Samuel ◽

Crystal L Levesque

Keyword(s):

Growth Performance ◽

Phase 1 ◽

Dietary Treatment ◽

Experimental Period ◽

Phase 2 ◽

Deficient Diet ◽

Nursery Pigs ◽

Feeding Program ◽

Dietary Treatments ◽

Fecal Score

Abstract This experiment evaluated the effect of dietary protease supplementation on pig’s growth performance and post-weaning diarrhea in a 43-d trial. A total of 936 weaned pigs [21 d of age, initial BW 5.87 ± 0.31 kg] were used in a 2 × 2 factorial design (2 nutritional levels × 2 protease levels). The nutritional levels corresponded to a standard feeding program and a reduced feeding program (15% lower SID Lys) and without or with protease supplementation (0.0125% inclusion). Piglets were distributed in 36 pens with 26 pigs per pen and 9 replicates per dietary treatment. Pigs received a common Phase 1 diet and experimental diets in Phase 2, 3, and 4, (0.91, 3.4, 4.5, and 24.0 kg/pig, respectively). Phytase was included at 2,500 U in Phase 2 – 4, all diets met energy and AA:Lys requirements for nursery pigs. Feed intake and body weight were determined every 2 weeks. A pen fecal score assessment was performed at d7, 10, and 14. Performance data were analyzed using the PROC MIXED procedure and fecal score data using the PROC FREQ procedure (SAS Inst., Inc., Cary, NC). No significant interactions (P > 0.05) were observed between dietary treatments. From d15 to 43 and during overall experimental period, pigs fed standard diets had greater BW, ADG, and G:F (P < 0.05) than pigs fed deficient diets (Table 1). On d7, pigs fed deficient diet had more (X2 < 0.05) soft and watery feces, and consequently less normal feces compared to pigs fed standard diets (Figure 1). On d7, 10, and 14, pigs fed diets with protease supplementation had more (X2 < 0.05) normal feces compared to control pigs. Overall, effects of dietary protease supplementation on growth performance were not expressive under the conditions of this study but protease addition benefited intestinal health of nursery pigs.

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PSVI-14 Blend of Additives in Nursery Pig Diets as an Antibiotics Substitute Increases the Growth Performance and Intestinal Health

Journal of Animal Science ◽

10.1093/jas/skab054.328 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 201-201

Author(s):

Sudario Roberto Silva Junior ◽

Rhuan F Chaves ◽

Maíra Resende ◽

Ygor Henrique De Paula ◽

Kenio De Gouvêa Cabral ◽

...

Keyword(s):

Growth Performance ◽

Animal Feed ◽

Block Design ◽

Average Daily Gain ◽

Basal Diet ◽

Intestinal Health ◽

Total Period ◽

Nursery Pigs ◽

Randomized Block Design ◽

Diarrhea Incidence

Abstract Antibiotics growth promoting (AGP) in animal feed has been restricted. With this, several additives have been tested in order to replace AGP and ensure intestinal health. The objective of this study was to evaluate the effects of a blend of additives on growth performance, diarrhea incidence and cecal content microbiota of nursery pigs. A total of 576 weaned pigs (6.21 ± 1.16 kg) were assigned into 4 treatments (12 replicates) in randomized block design: basal diet without additives (NC); basal diet with 120 ppm of halquinol (PC); NC + 0,05% blend of lactic acid (3,5%), benzoic acid (2,5%), flavonoids (3,5%) (B5); NC+ 0,1% of same blend (B10). The pigs were weighed 0 and 42 days into the experiment. On day 10, one animal per pen (10 replicates) was euthanized for microbiota analyses. The diarrhea incidence was calculated by the percentage of visual pens with diarrhea, by day, in the period. The growth performance was analyzed by GLIMMIX procedure and the means were compared with Tukey test (P < 0.050) (SAS, 2009). Diarrhea incidence was analyzed by GENMOD procedure (SAS, 2009). The Kruskal Wallis test was used to compare microbial biodiversity (P < 0.050) by statistical program STAMP. The final weight (P = 0.010) and average daily gain (P = 0.001) of B10 and PC were higher than NC. Regarding diarrhea, it was observed that the B5 and B10 had lower incidence when compared to other groups in the total period. In the microbiome analyses, it was observed that the B5, B10, and PC groups had lower (P = 0.021) biodiversity when compared to the NC piglets. However, the B5 and B10 group had higher biodiversity when compared to the PC group. In conclusion, the supplementation with 0,1% blend of additives can be a substitute for AGP.

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268 In-feed Antibiotics Elicit Intestinal Integrity Modifications Early in Post-weaning Life

Journal of Animal Science ◽

10.1093/jas/skab054.158 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 99-99

Author(s):

Jessica M Johnson ◽

Emma T Helm ◽

Nicholas K Gabler ◽

Eric R Burrough ◽

Carson M De Mille

Keyword(s):

Growth Performance ◽

Fixed Effects ◽

Ex Vivo ◽

Phase 1 ◽

Physiological Mechanisms ◽

Nursery Pigs ◽

Ussing Chambers ◽

Intestinal Integrity ◽

And Function ◽

Dietary Treatments

Abstract The physiological mechanisms by which in-feed antibiotics improve pig growth performance are largely unknown. One proposed mode of action is improvements in intestinal integrity and function. Therefore, the objective of this study was to test the hypothesis that in-feed therapeutic and sub-therapeutic antibiotics would improve intestinal integrity and function in nursery pigs. Twenty-four weaned pigs (6.1±1.1 kg BW) were randomly allotted to individual pens and assigned one of three dietary treatments as follows (n = 8 pigs/trt): 1) control, no antibiotics (CON), 2) CON + sub-therapeutic chlortetracycline [40 ppm in feed (sCTC)], and 3) CON + chlortetracycline-tiamulin [400 ppm + 35 ppm, respectively (CTCDen)]. The study consisted of two consecutive 14 d phases. Chlortetracycline-tiamulin was only fed in phase 1, sCTC was fed in both phases. Phase 1 and 2 ADG, ADFI, and G:F were determined. After 28 d, ileal and colonic ex vivo intestinal integrity was assessed via transepithelial resistance (TER) and macromolecule flux (FD4) in modified Ussing chambers. All data were analyzed for the fixed effects of treatment and start BW as a covariate. In phase 1, compared with CON and sCTC, CTCDen tended to have greater ADG (0.28, 0.31, and 0.33 kg/d, respectively, P = 0.10) and ADFI (0.28, 0.30, and 0.35 kg/d, respectively, P = 0.09). No differences in phase 1 G:F were observed (P = 0.11). Phase 2 ADG, ADFI, and G:F did not differ (P > 0.10). Further, ileal TER and FD4 did not differ (P > 0.10). Colonic TER tended to be increased in sCTC compared with CON and CTCDen (78, 56, and 59 Ω/cm2, respectively, P = 0.07). Compared with CON, colonic FD4 flux was decreased in sCTC and CTCDen by 35–40% (P = 0.03). Altogether, these data indicate that in-feed antibiotics improve colon integrity early in production which may contribute to improved growth performance.

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129 Effects of a multi-strain Bacillus subtilis-based direct-fed microbial on growth performance, nutrient digestibility, and colonic pH in diets fed to weanling pigs

Journal of Animal Science ◽

10.1093/jas/skaa278.185 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 101-102

Author(s):

Jaron R Lewton ◽

Adrienne Woodward ◽

Ronny Moser ◽

Kyan M Thelen ◽

Adam J Moeser ◽

...

Keyword(s):

Amino Acids ◽

Bacillus Subtilis ◽

Growth Performance ◽

Distal Colon ◽

Nutrient Digestibility ◽

Nursery Pigs ◽

Fiber Digestion ◽

Males And Females ◽

Dietary Treatments ◽

The Impact

Abstract A study was conducted to evaluate the effects of a multi-strain Bacillus subtilis-based direct-fed microbial (DFM) on apparent digestibility and colonic pH of nursery pigs. Eighty pigs, of equal number of barrows and gilts (initial BW: 6.99 ± 1.67 kg), were weaned at 21 ± 1 d and randomly allotted to sixteen pens, with five pigs per pen. Two dietary treatments were implemented, a basal control (CON) and a control plus DFM (DFM). Both diets were corn, soybean meal, and distillers dried grains based, formulated to meet all or exceed all nutritional requirements, and manufactured on site. Diets were fed for 42 days. Performance measures were recorded weekly. On d 21 and 42 of the experiment, one pig per pen was randomly selected and euthanized, with equal number of males and females represented. Digestibility of specific nutrients was evaluated within the duodenum, jejunum, ileum, ascending and distal colon. There were no overall differences in growth performance. Overall means ± SD were 0.51 ± 0.05 kg/d, 0.79 ± 0.05 kg/d and 0.66 ± 0.05 for ADG, ADFI, and G:F, respectively. Digestibility of tryptophan within the jejunum tended (P = 0.06) to increase with addition of DFM, as did cysteine (P = 0.12) and methionine (P = 0.10). The analysis also suggested that the impact of the DFM on the digestibility of amino acids may be early in the nursery phase. The pH of contents in ascending colon, a possible indicator of varied fiber digestion, did not differ. Likewise, no differences were observed between treatment in apparent total tract nitrogen and energy digestibility (analysis of distal colon contents). The addition of a multi-strain Bacillus subtilis-based DFM appears to impact digestibility of select amino acids depending upon location in the gastrointestinal tract.

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