scholarly journals Silver Nanoparticles AgNPs Preparation by Chemical Method to Obtain Optimal Characteristics of The Pharmaceutical Form and Study Their Effect on The Leishmania Parasite in Laboratory: تحضير جسيمات الفضة النانوية AgNPs بالطريقة الكيميائية للحصول على الخصائص المثلى للشكل الصيدلاني المعتمد ودراسة تأثيرها على طفيلي الليشمانيا مختبريا

2021 ◽  
Vol 5 (3) ◽  
pp. 41-28
Author(s):  
Rama Muhamad Sattout, Ahmad Kamari, Muhamad Muayyad Bilal Rama Muhamad Sattout, Ahmad Kamari, Muhamad Muayyad Bilal
Keyword(s):  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Leishmania Parasite ◽  
Force Microscopy ◽  
Atomic Force ◽  
Yellow Color ◽  
Artificial Environment ◽  
Pharmaceutical Form ◽  
Average Size ◽  
Hierarchical Morphology

This study aimed first to prepare silver nanoparticles AgNPs in a chemical reduction, The silver nanoparticles AgNPs properties were characterized using spectroscopy of Vision and UV- Visible (UV- Vis), and atomic force microscopy (AFM) and a ZetaSizer device. This study also aimed to study the results effect of preparing Silver nanoparticles on the Leishmania parasite using an artificial environment. The results of the spectrophotometer of the secondary silver solution with yellow color, showed maximum absorption at a wavelength of 400 mm, while the image of atomic force microscope (AFM) showed the hierarchical morphology shape of the prepared Silver nanoparticles with an average size of (10- 30 nm). Silver secondary particles were estimated with a diameter ranging from (12- 60 nm) and with good dispersion in water by ZetaSizer device. The results illustrated that the number of Promastigotes of the Leishmania parasite decreased after adding the Silver nanoparticles AgNPs in varying proportions, as the concentration of 500 μg/ml had the highest effect after 72 hours from incubation, and the percentage of remaining parasites was 5.2% comparing with the control percentage of 14.99%, while the value of IC50 was 253.075 μg/ml, The value of R2 = 0.8809, slope = 0.0478 after 24 hours from incubation.

scholarly journals Synthesis and Photonics Applications of Afzelechin Conjugated Silver Nanoparticles

Coatings ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1295
Author(s):  
Shahid Ali ◽  
Muhammad Rahim ◽  
Perveen Fazil ◽  
Malik Shoaib Ahmad ◽  
Azeem Ullah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Band Gap ◽  
Band Gap Energy ◽  
Visible Absorption ◽  
Force Microscopy ◽  
Atomic Force ◽  
Uv Visible ◽  
Size And Morphology ◽  
Average Size ◽  
20 Nm

The silver nanoparticles were synthesized, functionalized with afzelechin and characterized using UV-Visible spectroscopy. A difference of 20 nm was observed in surface plasmon resonance of bare and functionalized silver nanoparticles which indicates afzelechin conjugation with silver nanoparticles. The atomic force microscopy (AFM) technique was used for the determination of the size and morphology of synthesized silver nanoparticles. The afzelechin conjugated silver nanoparticles were spherical and their sizes ranged from 3 to 10 nm with an average size of 8 nm while the bare silver nanoparticles were also spherical and their sizes ranged from 3 to 10 nm with an average size of 6 nm. The average sizes were also calculated by fitting their UV-Visible absorption spectra. Fitting is based on the Mie and Mie Gans models, which deduced that afzelechin conjugated silver nanoparticles were 96.5% spherical and 3.5% spheroidal with an average size of 5 nm while bare silver nanoparticles were 100% spherical with an average size of 4 nm. Both the fitting model as well as the AFM results showed a difference of 3 nm between the sizes of afzelechin conjugated silver nanoparticles while 2 nm differences was observed for bare silver nanoparticles. The band gap energy of afzelechin conjugated silver nanoparticles and bare silver nanoparticles were calculated via Tauc’s equation and were found to be 5.1 eV and 5.4 eV, respectively. A difference of 0.3 eV was observed in band gap energies of afzelechin conjugated silver nanoparticles and bare silver nanoparticles.

scholarly journals Green Synthesis of Silver Nanoparticles Using Hibiscus sabdariffa Leaves Extract

2021 ◽  
Vol 3 (1) ◽  
pp. 76-81
Author(s):  
Siti Husnaa Mohd Taib ◽  
Kamyar Shameli ◽  
Roshafima Rasit Ali ◽  
Zahra Izadiyan ◽  
Zatil Izzah Ahmad Tarmizi
Keyword(s):  
Silver Nanoparticles ◽  
Hibiscus Sabdariffa ◽  
Ag Nps ◽  
Tem Analysis ◽  
Force Microscopy ◽  
Atomic Force ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Size Of Particles ◽  
Average Size

The present paper reports the synthesis of silver nanoparticles (Ag-NPs) by a green method using Hibiscus sabdariffa (H. sabdariffa) leaves extract as reductant and stabilizer. The synthesized Ag-NPs were characterized by ultraviolet-visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). UV-vis spectrum of synthesized Ag-NPs showed a peak at 378 nm. TEM analysis revealed that the particles were spherical and irregular in shape and has average size around 56.52 nm. This structure and size of particles were confirmed by AFM analysis. The UV-vis and FTIR spectrum provides evidence of the presence of caffeic acid component as a representative biomolecule in stabilising the nanoparticles based on previous studies. Hence, this study advocates that H. sabdariffa have potential for synthesizing nanoparticles.

Antimicrobial Activity of Silver Nanoparticles Synthesized by Streptomyces Species JF714876

2012 ◽  
Vol 5 (1) ◽  
pp. 1638-1642 ◽  
Author(s):  
Vidyasagar G M ◽  
Shankaravva B ◽  
R Begum ◽  
Imrose ◽  
Sagar R ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Ions ◽  
Human Beings ◽  
Streptomyces Species ◽  
Force Microscopy ◽  
E Coli ◽  
Extracellular Synthesis ◽  
Atomic Force ◽  
Uv Visible

Microorganisms like fungi, actinomycetes and bacteria are considered nanofactories and are helpful in the production of nanoparticles useful in the welfare of human beings. In the present study, we investigated the production of silver nanoparticles from Streptomyces species JF714876. Extracellular synthesis of silver nanoparticles by Streptomyces species was carried out using two different media. Silver nanoparticles were examined using UV-visible, IR and atomic force microscopy. The size of silver nanoparticles was in the range of 80-100 nm. Antimicrobial activity of silver nanoparticle against bacteria such as E. coli, S. aureus, and dermatophytes like T. rubrum and T. tonsurans was determined. Thus, this study suggests that the Streptomyces sp. JF741876 can produce silver ions that can be used as an antimicrobial substance.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

Antimicrobial and Cytotoxic Activity of Silver Nanoparticles Stabilized by Natural Biopolymer Arabinogalactan

2020 ◽  
Vol 19 (04) ◽  
pp. 1950029
Author(s):  
A. G. Demchenko ◽  
V. S. Sadykova ◽  
A. V. Lyundup ◽  
N. E. Sedyakina ◽  
T. I. Gromovykh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Cytotoxic Activity ◽  
Chemical Reduction ◽  
Antitumor Agent ◽  
Escherichia Coli Bacteria ◽  
Trichoderma Citrinoviride ◽  
Average Size ◽  
Antibacterial And Antifungal ◽  
Dose Dependent

Silver nanoparticles were synthesized by chemical reduction of silver nitrate using arabinogalactan polysaccharide as a reducing agent and a stabilizer. The average size of nanoparticles, obtained by analyzing TEM-images, was 10.8[Formula: see text]nm; zeta potential [Formula: see text][Formula: see text]mV. A study of the sol by electron diffraction showed that silver in the sample is in metallic form. The resulting preparation of silver nanoparticles showed both antibacterial and antifungal activity. A pronounced antibacterial activity of silver nanoparticles was demonstrated both in relation to conditionally pathogenic gram-positive (Bacillus subtilis and B. coagulans) and gram-negative (Escherichia coli) bacteria. Silver nanoparticles also possess antifungal activity against macromycete Fomitopsis sp., as well as two strains of micromycetes Trichoderma citrinoviride and Fusarium sporotrichioides. Using the methods of light and fluorescence microscopy, MTT-analysis and Real-time cell analysis, the cytotoxic activity of silver nanoparticles was investigated on HepG2 human hepatocellular carcinoma cells. It was demonstrated that nanoparticles cause a suppression of cell metabolic and proliferative activity, as well as dose-dependent induction of cell death (average relative EC[Formula: see text] value was [Formula: see text]g/ml). The preparation of silver nanoparticles stabilized by arabinogalactan can be used in medicine, as a potential antimicrobial and antitumor agent.

scholarly journals Study the Effect of Water Content on the Structure of Electrochemically Prepared TiO2 Nanotubes

2022 ◽  
Author(s):  
Sara Al-Waisawy ◽  
Ahmed Kareem Abdullah ◽  
Hadi A. Hamed ◽  
Ali A. Al-bakri
Keyword(s):  
Water Content ◽  
Pure Titanium ◽  
Electrochemical Anodization ◽  
X Ray Diffraction ◽  
Force Microscopy ◽  
Atomic Force ◽  
Titania Nanotube Arrays ◽  
Anodization Process ◽  
Titania Films ◽  
Average Size

In this research, the pure titanium foil was treated in glycerol base electrolyte with 0.7 wt.% NH4F and a small amount of H2O at 17 V for 2 hours by electrochemical anodization process in order to prepare Titania nanotube arrays at room temperature (~25 ºC), different water content was added to the electrolyte as a tube enhancing agent. The high density uniform arrays are prepared by using organized and well aligned these tubes. The average size of tube diameter, ranging from 57 to 92 nm which found it increases with increasing water content, and the length of the tube ranging from 2.76 to 4.12 µm, also found to increase with increasing water content and ranging in size of wall thickness from 23 to 35 nm. A possible growth mechanism is presented. The X-ray diffraction (XRD), atomic force microscopy (AFM), and scanning electron microscopy (SEM) were utilized to study the structure and morphology of the Titania films.

scholarly journals Silver Nanoparticles Embedded in Natural Rubber Films: Synthesis, Characterization, and Evaluation ofIn VitroToxicity

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Caroline S. Danna ◽  
Dalita G. S. M. Cavalcante ◽  
Andressa S. Gomes ◽  
Leandra E. Kerche-Silva ◽  
Eidi Yoshihara ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Natural Rubber ◽  
Chinese Hamster ◽  
Cell Lineage ◽  
Microscopic Analysis ◽  
Visible Spectrum ◽  
Electron Microscopic ◽  
Force Microscopy ◽  
Atomic Force

Natural rubber (NR) films can reduce silver metal ions forming embedded metal nanoparticles, a process that could be described as green synthesis. The NR films acting as a reactor generate and incorporate silver nanoparticles (AgNPs). Organic acids and amino acids play a crucial role in the formation of AgNPs. The plasmon extinction obtained in the UV-visible spectrum shows the presence of nanoparticles in the film after dipping the NR film into a solution of silver nitrate at 80°C. Electron microscopic analysis confirms the presence of AgNPs in the NR film and characterization by atomic force microscopy shows a change in the roughness of the NR film with AgNPs. In addition, our preliminary results fromin vitrotoxicity studies (MTT and comet assays) of the NR films and NR films with silver nanoparticles (NR/Ag) show that they are not toxic to cell lineage CHO-K1 (cells from the ovary of a Chinese hamster), an important result for potential medical applications.

scholarly journals Hydrophilic Silver Nanoparticles Loaded into Niosomes: Physical–Chemical Characterization in View of Biological Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1177 ◽  
Author(s):  
Federica Rinaldi ◽  
Elena del Favero ◽  
Johannes Moeller ◽  
Patrizia Nadia Hanieh ◽  
Daniele Passeri ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Human Serum ◽  
Nile Red ◽  
Chemical Characterization ◽  
Entrapment Efficiency ◽  
Tween 20 ◽  
Biological Applications ◽  
Force Microscopy ◽  
Atomic Force ◽  
New Perspective

Silver nanoparticles (AgNPs) are widely used as antibacterial agents and anticancer drugs, but often their low stability limits their mass production and broad applications. The use of niosomes as a carrier to protect and envelop AgNPs gives a new perspective to solve these problems. In this study, AgNPs were functionalized with sodium 3-mercapto-1-propanesulfonate (3MPS) to induce hydrophilic behavior, improving loading in Tween 20 and Span 20 niosomes (NioTw20 and NioSp20, respectively). Entrapment efficiency was evaluated by UV analyses and is around 1–4%. Dimensions were investigated by means of dynamic light scattering (DLS) (<2RH> = 140 ± 4 nm and <2RH> = 251 ± 1 nm respectively for NioTw20 + AgNPs and NioSp20 + AgNPs) and were compared with those by atomic force microscopy (AFM) and small angle X ray scattering (SAXS) analyses. Stability was assessed in water up to 90 days, and both in bovine serum and human serum for up to 8 h. In order to characterize the local structure of niosomes, SAXS measurements have been performed on Tween 20 and Span 20 empty niosomes and loaded with AgNPs. The release profiles of hydrophilic probe calcein and lipophilic probe Nile Red were performed in HEPES buffer and in human serum. All these features contribute to conclude that the two systems, NioTw20 + AgNPs and NioSp20 + AgNPs, are suitable and promising in the field of biological applications.

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