Metaplastic Changes in Odontogenic Cysts of the Jawbone: Their Significance and Relation to MUC Family Expression

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Harada H ◽  
◽  
Iizuka N ◽  
Nakatsuka SI ◽  
Shima M ◽  
...  
Keyword(s):  
Surface Layer ◽  
Potential Role ◽  
Morphological Changes ◽  
Bronchial Epithelium ◽  
Odontogenic Cysts ◽  
Cystic Lesions ◽  
Mucous Cells ◽  
Histological Alterations ◽  
Dentigerous Cysts ◽  
Columnar Cells

Odontogenic cysts are typical diseases that account for the majority of lesions that occur in the jawbone, among which radicular and dentigerous cysts are the most common. In some cases, metaplastic changes in these cysts result in the development of goblet-like mucous cells admixed with ciliated columnar cells. Odontogenic cysts are of little importance to emergent risk in normal conditions, but such histological alterations could be drastic and even confusing. We examined the expression of MUC family in these cystic lesions and investigated their relationship with histomorphological features. 6 cases of radicular and dentigerous cysts with obvious metaplastic changes were studied. Of these, 4 were male and 2 were female. One case occurred in the maxilla and 5 in the mandible. Immunohistochemically, almost the entire epithelium was positive for MUC1 and MUC4 in all cases, and the decapitation-like protrusions on the surface layer showed stronger expression. MUC5AC was selectively expressed in mucous cells, while MUC2 and MUC6 were negative. The lining epithelium with metaplastic changes closely resembled the bronchial epithelium, and MUC expression indicates a potential role in these morphological changes.

scholarly journals S100A8 calcium-binding expression in radicular and dentigerous cysts and in keratocystic odontogenic tumors

2008 ◽  
Vol 55 (2) ◽  
pp. 82-87
Author(s):  
Giovanna Iezzi ◽  
Adriano Piattelli ◽  
Corrado Rubini ◽  
Gaia Goteri ◽  
Vittoria Perrotti ◽  
...  
Keyword(s):  
Protein Expression ◽  
Calcium Binding ◽  
Potential Role ◽  
Neoplastic Transformation ◽  
Biological Behavior ◽  
Odontogenic Cysts ◽  
Keratocystic Odontogenic Tumor ◽  
Study Results ◽  
Dentigerous Cysts ◽  
Epithelial Layers

Introduction: Recently the term Keratocystic Odontogenic Tumor (KCOT) has been recommended for Odontogenic Keratocysts (OKC) to address the neoplastic nature of the lesion compared to radicular and dentigerous cysts. S100 are calcium-binding proteins involved in cell differentiation and inflammation, with a potential role in neoplastic transformation. Aim: The aim of this study was to evaluate whether S100A8 protein expression is different in KCOT compared to radicular cysts (RC) and dentigerous cysts (DC). Methods: A total of 84 consecutive odontogenic cysts, 34 RC, 25 DC, and 25 KCOT, were analyzed in this study. Results: Epithelial cells in KCOT cases were not immunoreactive for S100A8 except focally in cases associated with inflammation, while RC cases showed a variable positivity of all the epithelial layers from the basal to the superficial in 19/34 cases and DC cases showed a weak positivity of the intermediate and superficial layers in 7/25 cases. Conclusion: The lack of S100A8 protein expression seems to be observed more frequently in KCOT compared to RC and DC. This difference might be related to their neoplastic nature and a potential aggressive biological behavior for odontogenic cystic lesions.

Clinico-Radiological Study of Odontogenic Cysts in Mandibular Region from Agartala, Tripura

2021 ◽  
Vol 8 (37) ◽  
pp. 3350-3355
Author(s):  
Amit Lal Goswami ◽  
Manik Saha
Keyword(s):  
Dentigerous Cyst ◽  
Case Series ◽  
Odontogenic Cysts ◽  
Cystic Lesions ◽  
Radicular Cyst ◽  
Medical College ◽  
Dentigerous Cysts ◽  
Mandibular Region ◽  
Common Variety ◽  
Age Range

BACKGROUND Most common osseous destructive lesions effecting the jawbone are odontogenic cystic lesions, which are lined by epithelium, has been involved in the formation of teeth as the epithelial cell rest are present in jawbone after teeth formation. The three most common odontogenic cysts (OCs) are radicular cyst (RC), dentigerous cyst (DC) and the odontogenic keratocyst (OKC). Less common variety is the residual cyst. Odontogenic cysts are mainly developmental and inflammatory in origin. The objective of the present study was to find clinico-radiographic characteristics of odontogenic cysts in the mandible. METHODS This was a case series (descriptive) study on OCs of mandible conducted at Tripura Medical College and Dr BRAM Teaching Hospital, Hapania, Agartala, Tripura (W), India, over a period of 6 months from August 2019 to January 2020. Subjects showing clinico-radiographic features of OCs in mandible were included, subsequently confirmed by histopathological examinations. Usually orthopantomograms (OPGs) and intra oral peri apical radiograph (IOPAs) were done for radiological findings, computed tomography (CT) scan was required for examination of the size of lesion. Data were analysed using windows excel. RESULTS Diagnosis of OCs was confirmed in 20 patients, among them, males were (60 %) and female were (40 %). The age range of the patients was 17 - 65 years. Most common type of cyst diagnosed in the study was radicular cyst in 50 % cases followed by dentigerous cysts which were in 30 % cases, odontogenic keratocysts were in 15 % patients and others were 5 %. RCs were represented in higher percentage (50 %). Right side of mandible was the most effected side (60 %) than left side (40 %). Main finding of the study was, in every type of cystic lesions, males were recorded as predominant, and it occurred in early age group. CONCLUSIONS Main finding of the study was radicular cyst which was in higher percentage (50 %) and was being most common with association of carious tooth. KEYWORDS Odontogenic Cysts, Mandible, Finding

scholarly journals A Comparative Histochemical Study of Mucous Cells in Odontogenic Cysts

2013 ◽  
Vol 2013 ◽  
pp. 1-4
Author(s):  
Ridwaana Carim ◽  
Farzana Mahomed
Keyword(s):  
Neutral Type ◽  
Mucous Cell ◽  
Odontogenic Cysts ◽  
Type I ◽  
Type Ii ◽  
Mucous Cells ◽  
Diagnostic Challenge ◽  
Type Iii ◽  
Mucous Metaplasia ◽  
Dentigerous Cysts

The diagnosis of a glandular odontogenic cyst (GOC) on an incisional biopsy continues to remain a diagnostic challenge for the histopathologist. A marker for distinguishing GOC from odontogenic cysts with mucous metaplasia is thus needed in routine pathology practice. This study aimed to determine the histochemical composition of the mucous cells in the GOC and to compare the findings with the mucous cells in odontogenic cysts that show overlapping histomorphological features with the GOC. GOCs (), dentigerous cysts (DCs) (), and radicular cysts (RCs) () with mucous metaplasia were stained using the combined alcian blue(pH 2.5)-PAS histochemical technique. The cysts were evaluated for the frequencies of acidic- (type I), neutral- (type II) and mixed- (acidic and neutral (type III)) mucin containing cells. Significant differences were found between the levels of type I, type II, and type III mucous cells within the 3 cyst types, GOC (), DC (), and RC (), which all showed a predominance of type III mucous cells. There were, however, no significant differences for each mucous cell type between the 3 cyst types. GOC thus appears to share the same histochemical mucin phenotype with the mucous cells in DC and RC.

scholarly journals Expression of SOX2 and OCT4 in odontogenic cysts and tumors

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Ekarat Phattarataratip ◽  
Tarit Panitkul ◽  
Watunyoo Khodkaew ◽  
Pattarapong Anupuntanun ◽  
Jirapat Jaroonvechatam ◽  
...  
Keyword(s):  
Cell Types ◽  
Odontogenic Cysts ◽  
Positive Staining ◽  
Stem Cell Markers ◽  
Aberrant Expression ◽  
Future Studies ◽  
Sox2 Expression ◽  
Cells Of Origin ◽  
Dentigerous Cysts ◽  
Patient Prognosis

Abstract Background Aberrant expression of stem cell markers has been observed in several types of neoplasms. This trait attributes to the acquired stem-like property of tumor cells and can impact patient prognosis. The objective of this study was to comparatively analyze the expression and significance of SOX2 and OCT4 in various types of odontogenic cysts and tumors. Methods Fifty-five cases of odontogenic cysts and tumors, including 15 ameloblastomas (AM), 5 adenomatoid odontogenic tumors (AOT), 5 ameloblastic fibromas (AF), 5 calcifying odontogenic cysts (COC), 10 dentigerous cysts (DC) and 15 odontogenic keratocysts (OKC) were investigated for the expression of SOX2 and OCT4 immunohistochemically. Results Most OKCs (86.7 %) and all AFs expressed SOX2 in more than 50 % of epithelial cells. Its immunoreactivity was moderate-to-strong in all epithelial cell types in both lesions. In contrast, SOX2 expression was undetectable in AOTs and limited to the ameloblast-like cells in a minority of AM and COC cases. Most DCs showed positive staining in less than 25 % of cystic epithelium. Significantly greater SOX2 expression was noted in OKC compared with DC or AM, and in AF compared with COC or AOT. OCT4 rarely expressed in odontogenic lesions with the immunoreactivity being mild and present exclusively in OKCs. Conclusions SOX2 is differentially expressed in odontogenic cysts and tumors. This could be related to their diverse cells of origin or stages of histogenesis. The overexpression of SOX2 and OCT4 in OKC indicates the acquired stem-like property. Future studies should investigate whether the overexpression of OCT4 and SOX2 contributes to the aggressive behaviors of the tumors.

scholarly journals Differential Expression of Claudin in Odontogenic Cysts

2021 ◽  
Author(s):  
Ekarat Phattarataratip ◽  
Kraisorn Sappayatosok
Keyword(s):  
Pairwise Comparison ◽  
Odontogenic Cyst ◽  
Odontogenic Cysts ◽  
Positive Staining ◽  
Pathogenic Role ◽  
Dentigerous Cysts ◽  
Different Types ◽  
Level Of Significance ◽  
Post Hoc ◽  
Spearman’S Correlation

Abstract Objective This study aimed to analyze claudin-1, -4, and -7 expression in different types of odontogenic cysts (odontogenic keratocysts [OKCs], dentigerous cysts [DCs], calcifying odontogenic cysts [COCs], and radicular cysts [RCs]) as well as its association with OKC recurrence. Materials and Methods Seventy samples of odontogenic cysts samples were immunohistochemically stained to detect claudin-1, -4, and -7 expression. Patient information and OKC recurrence data were recorded. The staining was analyzed semiquantitatively and categorized based on the pattern and percentage of positively stained cystic epithelial cells. Statistical Analysis Expression of different claudins between groups was analyzed using the Kruskal–Wallis test with Dunn's test, followed by post hoc pairwise comparison. The association between claudin expression and OKC recurrence was analyzed by the Mann–Whitney U test. Correlations among claudin expression were examined with Spearman's correlation coefficient. Level of significance was at p < 0.005. Results Claudin-1 was widely expressed in every odontogenic cyst. Most DCs (50%) expressed claudin-1 in more than 75% of cells, as did RCs (65%), while most OKCs (50%) expressed claudin-1 in 26 to 50% of cells. Most COCs (50%) expressed claudin-1 in 51 to 75% of cells. Every sample of OKC and RC was positive for claudin-4, but no sample showed staining in more than 51% of cells. Every odontogenic cyst was positive for claudin-7. DCs (35%), OKCs (55%), and RCs (40%) mostly showed staining in 26 to 50% of cells. High claudin-1 expression was shown in COCs, DCs, and RCs, while low expression of claudin-4 was shown in every odontogenic cyst. For claudin-7, the expression is high only in COCs. Claudin-1 and -4 was significantly different among each odontogenic cyst. High expression of claudin-1 was correlated with OKC recurrence. The correlations of claudin-1 with claudin-7 expression and claudin-4 with claudin-7 expression were significant in DCs. In COCs, claudin-1 and claudin-7 expression was significantly correlated. Conclusions The expression of claudin-1, -4, and -7 was present in every odontogenic cyst, but the proportion of positive staining cells was different. Expression of claudin-1 is associated with OKC recurrence. Dysregulation of claudin expression may play a pathogenic role in cyst pathogenesis.

Retrospective 3D analysis of bone regeneration after cystectomy of odontogenic cysts

2020 ◽  
Vol 28 (6) ◽  
pp. 1141-1155
Author(s):  
Mayte Buchbender ◽  
Birte Koch ◽  
Marco Rainer Kesting ◽  
Ragai Edward Matta ◽  
Werner Adler ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Computer Tomography ◽  
Evaluation Method ◽  
Defect Size ◽  
Odontogenic Cysts ◽  
3D Analysis ◽  
Dentigerous Cysts ◽  
The Mean ◽  
3D Evaluation

BACKGROUND/OBJECTIVE: In this retrospective study, we aimed to investigate a new 3D evaluation method for evaluating bone regeneration after cystectomy of odontogenic cysts. METHODS: The study included 26 patients who underwent cystectomies between 2012 and 2017 and had received either fillings or non-fillings with autologous iliac crest. Bony regeneration was analyzed using 3D imaging software and comparing identical regions of interest (ROIs) that were determined by exact overlays of the postoperative cone beam computer tomography (CBCT) or computer tomography (CT) images. Outcome measures, including volume changes according to the defect size and configuration, patient age, the entity and distribution of the cysts, were collected. RESULTS: Twenty-six patients (5 women and 21 men) had 30 defects, including nine keratocysts, seven radicular cysts and 14 dentigerous cysts. A total of 73% of the defects were in the mandible. The mean 3D follow-up time was 12 months. According to the 3D evaluation of bony regeneration, the defect size and configuration showed no significant differences between the groups (filled or non-filled with 15 defects per group). CONCLUSIONS: By establishing a standardized 3D method for evaluating bone regeneration, healing can be better monitored and evaluated.

scholarly journals Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus)

2015 ◽  
Vol 59 (3) ◽  
Author(s):  
H. Zhang ◽  
X. Guo ◽  
S. Zhong ◽  
T. Ge ◽  
S. Peng ◽  
...  
Keyword(s):  
Mucous Cell ◽  
Single Layer ◽  
Esophageal Mucosa ◽  
Mucous Cells ◽  
Esophageal Epithelium ◽  
Andrias Davidianus ◽  
Transmission Electron ◽  
Chinese Giant Salamander ◽  
Columnar Cells ◽  
Dense Vesicles

The Chinese giant salamander belongs to an old lineage of salamanders and endangered species. Many studies of breeding and disease regarding this amphibian had been implemented. However, the studies on the ultrastructure of this amphibian are rare. In this work, we provide a histological and ultrastructural investigation on posterior esophagus of Chinese giant salamander. The sections of amphibian esophagus were stained by hematoxylin &amp; eosin (H&amp;E). Moreover, the esophageal epithelium was observed by transmission electron microscopy (TEM). The results showed that esophageal epithelium was a single layer epithelium, which consisted of mucous cells and columnar cells. The esophageal glands were present in submucosa. The columnar cells were ciliated. According to the diverging ultrastructure of mucous vesicles, three types of mucous cells could be identified in the esophageal mucosa: i) electron-lucent vesicles mucous cell (ELV-MC); ii) electron-dense vesicles mucous cell (EDV-MC); and iii) mixed vesicles mucous cell (MV-MC).

scholarly journals Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae

2017 ◽  
Vol 61 (3) ◽  
Author(s):  
Gianluca Accogli ◽  
Giovanni Scillitani ◽  
Donatella Mentino ◽  
Salvatore Desantis
Keyword(s):  
Surface Layer ◽  
Lectin Histochemistry ◽  
The Body ◽  
Octopus Vulgaris ◽  
Mucous Cells ◽  
Cell Type ◽  
Skin Mucus ◽  
The Common ◽  
Common Octopus

<p>The O<em>ctopus vulgaris</em> farming is impaired by the high mortality of the paralarvae during the first month of life. Several factors have been investigated in this regard, but no data exist on the body surface mucus, which represents the interface with the outside environment. This study included morphometric analysis and glycoconjugates characterization of skin mucus in reared <em>Octopus vulgaris</em> paralarvae during the first month of life. Four types of mucous cells were distinguished:  mucous 1 (m1) and mucous 2 (m2) cells were scattered in the mantle epidermis, mucous 3 (m3) and mucous 4 (m4) in the epithelium surrounding the sucker. Except for the presence of fucosylated and neutral glycoconjugates in all mucous cells, each cell type expressed a characteristic glycopattern. m2 and m4 contained also suphate and acid non-sulphate glycans, m3 lacked suphate glycoproteins. Lectin histochemistry showed that mantle mucous cells (m1, m2) expressed GlcNAc and lactosamine terminating glycans. m2 also contained GalNAc terminal or penultimate to sialic acid. m3 was distinguished by mannosylated glycans terminating with lactosamine and m4 by α2,6 sialoglycans. Glycoproteins terminating with lactosamine, Galβ1,3GalNAc, and α1,6-linked fucose were a common feature of paralarvae surface layer. Morphometry revealed a significant decrease of m1 and m2 abundance during the first month of life, afterwards the reared paralarvae died. Since the glycopattern did not change during the investigated period, the mantle mucous cells abundance could be related to the <em>Octopus vulgaris</em> paralarvae survival. </p>

scholarly journals A Comparison of Ki67, Syndecan-1 (CD138), and Molecular RANK, RANKL, and OPG Triad Expression in Odontogenic Keratocyts, Unicystic Ameloblastoma, and Dentigerous Cysts

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Luisana Brito-Mendoza ◽  
Ronell Bologna-Molina ◽  
María Esther Irigoyen-Camacho ◽  
Guillermo Martinez ◽  
Celeste Sánchez-Romero ◽  
...  
Keyword(s):  
Proliferation Rate ◽  
Proliferation Index ◽  
Cystic Lesions ◽  
Rankl Expression ◽  
Therapeutic Approaches ◽  
Ki 67 ◽  
Unicystic Ameloblastoma ◽  
Dentigerous Cysts ◽  
Lower Expression ◽  
Destructive Potential

Background and Objective. Reduced expression of syndecan-1 (CD138), increased proliferation index, and modifications in the expression of the molecular RANK/RANKL/OPG triad are related to an intensified potential of aggressiveness and invasion of diverse tumors and cysts. The aim was to compare the expression of Ki-67, CD138, and the molecular triad RANK, RANKL, and OPG in odontogenic keratocysts (OKC), unicystic ameloblastomas (UA), and dentigerous cysts (DC). Methods. Immunohistochemistry for Ki-67, CD138, RANK, RANKL, and OPG was performed in 58 odontogenic cystic lesions (22 OKC, 17 DC, and 19 UA). Results. A higher expression of Ki-67 was identified in OKC as compared to UA (p<0.0001). UA exhibited a greater loss of CD138 expression versus OKCs (p>0.0034). RANKL was expressed higher in the epithelium (p=0.0002) and in the stroma (p=0.0004) of UA. DC had a lower expression of these markers. Conclusion. Higher RANKL expression together with the reduction on CD138 expression in UA could be linked to a greater invasive and destructive potential, while the increased proliferation rate observed in OKC could be related to its continuous intrabony growth. The expansion of DC does not seem to be related to such factors, justifying the different therapeutic approaches proposed for each of these entities.

Giant dentigerous cyst occupying the right hemimandible

2002 ◽  
Vol 10 (4) ◽  
pp. 171-174
Author(s):  
Uğur Koçer ◽  
H Mete Aksoy ◽  
YiğIt Ö TiftikçioğLu ◽  
Dilek Ertoy ◽  
Önder Karaaslan
Keyword(s):  
Young Patient ◽  
Bone Grafting ◽  
Present Report ◽  
Dentigerous Cyst ◽  
Odontogenic Cysts ◽  
Surgical Removal ◽  
Dentigerous Cysts ◽  
Primary Bone ◽  
The Right

Dentigerous cysts are the second most common odontogenic cysts of the mandible. They may vary in size from 2 cm to more than 10 cm. In the present report, a dentigerous cyst with massive involvement of the right half of the mandible in a young patient is presented. For cosmetic reasons and long term risks these cysts must be treated. Surgical removal is the preferred modality and a submandibular route should be chosen in large lesions. Bony cavities may be decreased in volume by using osteoperiosteal flaps, but there is no need for primary bone grafting, even for large defects.

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