scholarly journals Isolation of chemical constituents and In-Vitro screening of Plectranthus mollis Spreng for anthelmintic activity

2020 ◽  
Vol 11 (2) ◽  
pp. 1519-1525
Author(s):  
Haripriya G ◽  
Duraiswamy B ◽  
Dhamodaran P
Keyword(s):  
Chemical Constituents ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Antimicrobial Treatment ◽  
Flash Chromatography ◽  
Total Phenolic ◽  
Hydroalcoholic Extract ◽  
Aerial Parts ◽  
Isolated Compound

The human being are the most affected with diseases than any other animal species. For a long time, almost all the medicines were extracted from plants and, many herbal drugs were used by different civilization and many of them are still used. Tannins and flavonoids are the chemical constituents in the plants that produces the anti-helminthic activity. In traditional medicine, Plectranthus mollis has been used against snakebites, respiratory stimulant and cure for hemorrhage, antimicrobial, treatment of mental retardation and rheumatism. Moreover, Plectranthus mollis is reported to have a high content of tannins and flavonoids. Depending on this, helminthic activity were proposed.Dried aerial parts of the hydroalcoholic extract of Plectranthus mollis are subjected to preliminary tests for the identification of various active constituents present in the extract. Estimation of total phenolic content were determined calorimetrically using Folin-Ciocalteu method and total flavonoid content were determined by aluminum chloride colorimetric technique for the hydroalcoholic extract of Plectranthus mollis. The antioxidant activity of the hydroalcoholic extract of the plantwere carried out by DPPH radical scavenging assay. The anthelmintic activity of dried aerial parts of the hydroalcoholic extract of Plectranthus mollis are evaluated on adult Pheretima Posthuma (Indian earthworm).The chemical compound present in dried aerial parts of hydroalcoholic extract of Plectranthus mollis were isolatedby flash chromatography. The isolated compound were detected by HPTLC technique and confirmed by GC-MS analysis.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF ANACARDIUM OCCIDENTALE, ACHYRANTHES ASPERA, AND AEGLE MARMELOS

2019 ◽  
pp. 202-205
Author(s):  
BRIJYOG ◽  
LALITESHWAR PRATAP SINGH ◽  
SUSHIL KUMAR ◽  
SHWETA VERMA
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Anacardium Occidentale ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Aegle Marmelos ◽  
Hydroalcoholic Extract ◽  
Achyranthes Aspera ◽  
Aerial Parts

Objective: Anacardium occidentale, Achyranthes aspera, and Aegle marmelos are the common plants found in North and South India. The present study identifies the active phytochemicals and antioxidant properties in various extracts derived from dried bark, whole aerial parts, and leaves of the above plants. Materials and Methods: Phytochemical screening included extracts of pet ether, hydroalcoholic, and aqueous. The antioxidant activity was determined by measuring total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (RSA), and total flavonoid content (TFC). Results: The phytochemical screening of A. occidentale, A. aspera, and A. marmelos of dried bark, whole aerial parts, and leaves revealed the presence of flavonoids, phenols, tannins, and proteins. It also contains alkaloids and glycosides. Hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos showed highest TPC 0.125 mg/g, 0.256 mg/g, and 0.254 mg/g, respectively. TFCs 0.094 mg/g, 0.145 mg/g, and 0.121 mg/g and highest DPPH RSA with the half maximal inhibitory concentration of 125 μg/mL, 105.58 μg/mL, and 98.89 μg/mL compare to the standard ascorbic acid of 21.65 μg/mL. Conclusion: This study showed that the hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos is potential source of natural antioxidants.

scholarly journals In VitroAntioxidant and Anticancer Activity of Flavonoid Fraction from the Aerial Parts ofCissus quadrangularisLinn. against Human Breast Carcinoma Cell Lines

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Vijayalakshmi ◽  
P. R. Kumar ◽  
S. Sakthi Priyadarsini ◽  
C. Meenaxshi
Keyword(s):  
Breast Cancer ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dry Weight ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Aerial Parts ◽  
Cissus Quadrangularis ◽  
Flavonoid Fraction

Aim. The present study aimed to isolate flavonoid fraction from the aerial parts ofCissus quadrangularisand to evaluate its antioxidant and anticancer potential usingin vitroassay system.Methods. Total phenolic and flavonoid contents were calculated for the drug. Flavonoid fraction was isolated using column chromatography and analysed using HPLC.In vitro, antioxidant activity of the ethanol extract and isolated flavonoid fraction was investigated by nitric oxide, DPPH and hydroxyl radical scavenging assays. Breast cancer (MCF 7) cell line was used as thein vitrocancer model for MTT assay.Result. The amount of total phenolic content and total flavonoid content in the ethanol extract showed 28.6 mg/g dry weight expressed as gallic acid equivalents, and 15.8 mg/g was expressed as quercetin equivalents, respectively. The tested extract showed good dose-dependent free radical scavenging property in all the models with the IC50values of 98 μg/mL, 125 μg/mL, and 96 μg/mL for ethanol extract and 10 μg/mL, 12 μg/mL, and 10 μg/mL for flavonoid fraction, respectively. The flavonoid fraction possess potent anticancer property against breast cancer cells (MCF7) with IC50value of 40 μg/mL.Conclusions. It can be concluded that the aerial part ofCissus quadrangularishas potential antioxidant and anticancer activities.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals In vitro thrombolytic, anthelmintic, anti-oxidant and cytotoxic activity with phytochemical screening of methanolic extract of Xanthium indicum leaves

2015 ◽  
Vol 10 (4) ◽  
pp. 854 ◽  
Author(s):  
Antara Ghosh ◽  
Sujan Banik ◽  
Md. Ariful Islam
Keyword(s):  
Cytotoxic Activity ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
Positive Control ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Oxidant

<p class="Abstract"><em>Xanthium indicum</em> is an important medicinal plant traditionally used in Bangladesh as a folkloric treatment. The current study was undertaken to evaluate thrombolytic, anthelmintic, anti-oxidant, cytotoxic properties with phytochemical screening of methanolic extract of <em>X. indicum</em> leaves. The analysis of phytochemical screening confirmed the existence of phytosetrols and diterpenes. In thrombolytic assay, a significant clot lysis was observed at four concentrations of plant extract compare to the positive control streptokinase (30,000 IU, 15,000 IU) and negative control normal saline. The extract revealed potent anthelmintic activity at different concentrations. In anti-oxidant activity evaluation by two potential experiments namely total phenolic content determination and free radical scavenging assay by 2, 2-diphenylpicrylhydrazyl (DPPH), the leaves extract possess good anti-oxidant property. In the brine shrimp lethality bioassay, the crude extract showed potent (LC<sub>50</sub> 1.3 μg/mL) cytotoxic activity compare to the vincristine sulfate as a positive control (LC<sub>50</sub> 0.8 μg/mL). </p><p> </p>

scholarly journals Preliminary Phytochemical Screening andIn VitroAntioxidant Activities ofParkinsonia aculeataLinn.

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sonia Sharma ◽  
Adarsh Pal Vig
Keyword(s):  
Plant Extracts ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Site Specific ◽  
Parkinsonia Aculeata ◽  
Butanol Extract

Butanol and hexane leaves extracts ofParkinsonia aculeataL. (Fabaceae) were assessed for its antioxidant potential byin vitromethods. Phytochemical analysis and antioxidant activity of plant extracts were studied using differentin vitroassays. UPLC analysis of extracts was carried out for the identification of chemical constituents. The total phenolic contents of the butanol and hexane leaf extract were 42 mgGAE/g and 34 mgGAE/g whereas flavonoid contents of these extracts were found to be 0.044 mgRE/g and 0.005 mgRE/g, respectively. Among both extracts, butanol extract shows maximum inhibition (%) of 93.88%, 80.02%, 52.06%, 94.68%, and 69.37% in DPPH, non-site-specific and site-specific, FTC, and TBA assays and absorbance of 0.852 and 0.522 in reducing power and CUPRAC assay at the highest concentration tested. The FRAP and TAC values of butanol extract were found to be 678 μM Fe(II)/g and 36 mgAAE/100 mg. UPLC analysis of extracts revealed the presence of various polyphenols. The tested plant extracts were found to possess potent antioxidant and free radical scavenging activity which may be due to the presence of flavonoids and polyphenols.

scholarly journals In-vitro study of antiproliferative and anthelminthic property of medicinal plants of Kokrajhar, Assam

2021 ◽  
Author(s):  
Ananta Swargiary ◽  
Mritunjoy Kumar Roy ◽  
Akalesh Kumar Verma
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Anthelmintic Activity ◽  
In Vitro Study ◽  
Total Phenolic ◽  
First Choice ◽  
Different Doses

Abstract People living in far-flung areas of the world, especially ethnic tribal people practice traditional medicine as the first choice of disease treatment. The present study investigates the antioxidant, cytotoxicity, and anthelmintic activity of four medicinal plants traditionally used by tribal communities of Bodoland Region of Assam. Total phenolic and flavonoid content was estimated following spectrophotometry method. Antioxidant activity was measured by total antioxidant assay, FRAP, DPPH, ABTS, and TBARS assay. Antiproliferative and apoptosis-inducing activity of plants were carried out in DL cells. Cells were treated for 24 h with different doses of plant extracts. Furthermore, anthelmintic study was carried out by treating the helminth parasite at different doses of plant extracts. Phytochemical and antioxidant study showed rich TPC, TFC, and free radical scavenging activity in H. japonicum and H. sibthorpioides. Both the antiproliferative and anthelmintic activity showed a dose-dependent efficacy in all the plants. H. japonicum showed the strongest anthelmintic activity with LC50 212.78 µg/mL followed by H. sibthorpioides (5.36 mg/mL), C. halicacabum (13.40 mg/mL), and A. scholaris (18.40 mg/mL). On the other hand, H. sibthorpioides showed stronger antiproliferative and apoptosis-inducing activity compared to other plants. The study observed a positive correlation between the antioxidant property and antiproliferative and anthelmintic activities of the plants. We, therefore, conclude that the secondary metabolites along with antioxidant molecules may have combined effects contributing the antiproliferative and anthelmintic activity of the plants.

scholarly journals Nutritional potential of selected species of Arisaema Mart. from Nepal

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Laxmi Dhakal ◽  
Biva Aryal ◽  
Giri Joshi ◽  
Deepak Pant
Keyword(s):  
Phosphorus Content ◽  
Radical Scavenging Activity ◽  
Starch Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Aerial Parts ◽  
Dpph Radical Scavenging ◽  
Nutritional Potential ◽  
Made In

Abstract. Dhakal L, Aryal B, Joshi GP, Pant DR. 2020. Nutritional potential of selected species of Arisaema Mart. from Nepal. Biodiversitas 21: 5703-5709. Species of Arisaema Mart. are believed to be poisonous, but few species are used as food in different communities. Therefore, an assessment of nutritional properties of six selected taxa of Arisaema including three edible taxa was made in order to find out their nutritional potential. Methanolic or aqueous extracts of samples (rhizomes and aerial parts) were analyzed for various nutritional and anti-nutritional parameters. On nutritional basis, the total starch content and total reducing sugar were found highest (25.71±0.04mg/g powder) and (46.73±0.06 mg/g extract), respectively in aerial parts of A. echinatum. Total protein and phosphorus content was highest in rhizome of A. tortuosum var. curvatum. Similarly, extracts of aerial parts of A. tortuosum var. curvatum also possessed highest amount of total phenolic content (52.67±0.24mg GAE/g), total flavonoid content (14.13 ± 0.03 mg QE/g), and also demonstrated best antioxidant activity (IC50, 108.59±0.54 µg/mL) in terms of DPPH radical scavenging activity among the selected taxa. Inhibition of α-amylase and α-glucosidase in vitro was found to be highest (78.82 %) in rhizome extracts of A. echinatum and extracts of aerial parts of A. concinnum (34.82 %), respectively.

Phytochemical screening, in vitro antioxidant and enzyme inhibitory proprieties and acute toxicity of extracts from the aerial parts of Ephedra nebrodensis, source of bioactive compounds

2021 ◽  
Vol 24 ◽  
Author(s):  
Meriem Hamoudi ◽  
Djouher Amroun ◽  
Sabrina Boutefnouchet ◽  
Chawki Bensouici ◽  
Samira Kaoula ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Total Phenolic ◽  
Aerial Parts

Background: Due to the strong association between the chemistry of medicinal plants and their biological properties, it is important to determine their phytochemical composition to justify experimental tests. Objective: The aim of this study was to evaluate the in vitro antioxidant and the enzyme inhibitory properties and to identify the bioactive compounds present in the extracts of Ephedra nebrodensis growing in Algeria. Methods: Total phenolic and flavonoids content in these extracts were quantified by Folin-Ciocalteu and aluminum chloride methods. The antioxidant capacity was assessed using DPPH, ABTS, β-carotene/linoleic acid, CUPRAC and FRAP assays and in vitro cholinesterase activity against acetylcholinesterase and butyrylcholinesterase were evaluated. The chemical constituents of the extracts were analyzed by high-performance liquid chromatography coupled to mass spectrometric detection and gas chromatography coupled to mass spectrometric detection. For the acute toxicity study, extracts were administered to mice at single dose of 2 g/kg and 5 g/kg by gavage. Results: Plant extracts were rich in phenolic compounds. Ethyl acetate extract presented the highest phenolic (238.44 ± 1.50 µg GAE /mg of extract) and flavonoid (21.12 ± 0.00 µg QE /mg of extract) contents. Likewise, ethyl acetate extract showed potent radical scavenging and reducing properties. Ethanol: acetone extract showed inhibitory activity against acetylcholinesterase, and was a potent inhibitor of butyrylcholinesterase. In all extracts, flavonoids were the most abundant compounds. The phytochemical investigation showed the presence of alkaloids (ephedrine and pseudo-ephedrine). In the acute toxicity, the LD50 was superior to 5 g/kg body weight. There are not alterations in the histology of the liver and kidneys. Conclusion: This study demonstrated a good antioxidant potential and anticholinesterase activity of aerial parts of E. nebrodensis.

scholarly journals PHARMACOGNOSTICAL AND PHARMACOLOGICAL EVALUATION OF CRINUM LATIFOLIUM L.

2018 ◽  
Vol 10 (11) ◽  
pp. 17
Author(s):  
Puspendra Kumar Shukla ◽  
Manish Kumar ◽  
Ankita Misra ◽  
Bhanu Kumar ◽  
Ruchi Dwivedi ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Oleanolic Acid ◽  
Aerial Part ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Root Extract ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Radical Scavenging Assay

Objective: Pharmacognostical study along with the development of a quantitative HPTLC method for Crinum latifolium and evaluation of its traditional claims.Methods: Quantification of three marker compounds oleanolic acid, linoleic acid, and lupeol was done through HPTLC. In vitro antioxidant activity was determined by six different models, namely total phenolic and total flavonoid content, DPPH radical scavenging assay, ferric reducing power, antioxidant capacity and hydroxyl radical scavenging assay. In vitro antidiabetic activity was evaluated by α-amylase inhibition assay based on starch iodine and DNS method.Results: The content of oleanolic acid, linoleic acid, and lupeol were found to be higher in aerial parts like 0.015%, 0.048%, and 0.028% respectively, while in root extract 0.006%, 0.027% and 0.025% respectively on a dry weight basis. Free radical scavenging activity was done by DPPH assay, showing the IC50 value of 410±1.105 µg/ml in roots and 441.95±1.788 in aerial parts. In vitro antidiabetic potential of both the parts were assessed by starch iodine color assay and DNS method of alpha-amylase inhibition model. In 3,5 DNS assay, IC50 of extract from aerial parts was 282.21±2.151µg/ml whereas in root extract it was 193.33±2.45µg/ml. Iodine-starch assay of C. latifolium (aerial part) shown the IC50 value of 340.81±0.49 µg/ml and C. latifolium (root) of 74.64±1.28 µg/ml.Conclusion: The results indicate that the aerial parts of the plant possess more antidiabetic potential in comparison to the root. Thus, the aerial part can be used to get better results as a drug and roots can be used as an alternative.

GC‐MS and antioxidant capacity analyses of cowpea seeds oils

2013 ◽  
Vol 43 (2) ◽  
pp. 116-127 ◽  
Author(s):  
Muhammad Nadeem Asghar ◽  
Javaid Akhtar ◽  
Muhammad Shafiq ◽  
Iram Nadeem ◽  
Muhammad Ashfaq ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Cation ◽  
Vegetable Oils ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Content Type ◽  
Percent Inhibition ◽  
White Star

PurposeThe cowpea plant, being affordable and protein‐rich, is considered poor man's meat. The aim of this paper is to undertake a detailed investigation regarding in vitro total antioxidant capacity (TAC) and chemical constituents of the vegetable oils from seeds of this plant, taking its nutritional value into consideration.Design/methodology/approachVegetable oils of different indigenous cowpea varieties were obtained using soxhelt extraction assembly and subjected to GC‐MS analyses and various antioxidant assays including 2,2′‐azinobis(3‐ethylbenzothiazoline‐6‐sulpohonic acid) (ABTS) radical cation scavenging, ferr, 2.2′‐diphenyl‐1‐picrylhydrazil (DPPH) radical scavenging, total phenolic contents (TPC), lipid peroxidation inhibition, and iron chelation activity.FindingsVarious chemical constituents including different hydrocarbons, tocopherols, ketones, fatty esters, estragole and cedrene were identified. TPCs were found to be 5.439, 5.7279, 7.6126, 6.7573 and 10.0591 mg/L gallic acid equivalent for S.A. Dandy, Elite, White Star, CP‐386 and FBD Rawan varieties, respectively. Employing ABTS radical decoloration assay a significant linear correlation (R2=0.997, 0.996, 0.997, 0.996 and 0.997 for S.A. Dandy, Elite, White Star, CP‐386 and FBD Rawan varieties, respectively) was found between the percent inhibition of ABTS radical cation and the amount of vegetable oils. The percent inhibition of the Fe(II)‐Ferrozine complex formation was found to be 29.45, 53.76, 82.91, 86.59 and 57.87 for the same varieties, respectively.Originality/valueGC‐MS and standard in vitro antioxidative capacity analyses data clearly demonstrated the potency of the cowpea as antioxidant and radical scavenger plant which may be used as a good source of natural antioxidants. The plant seeds may prove a better and cost‐effective substitute of expensive food items.

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