scholarly journals Box-Behnken experimental design for extraction optimization of cytotoxic activity from Curcuma aeruginosa rhizome

2020 ◽  
Vol 11 (2) ◽  
pp. 1631-1637
Author(s):  
Rani Khoiriyah ◽  
Made Artika I ◽  
Waras Nurcholis
Keyword(s):  
Experimental Design ◽  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Pharmacological Activities ◽  
Solid Ratio ◽  
Bioactive Content ◽  
The Common

Curcuma aeruginosa is the common name of temuhitam in Indonesia, and the rhizome of parts plant had several pharmacological activities. Generally, pharmacological activities were associated with bioactive content in the extract of medicinal plants. Several factors can influence the bioactive extraction from medicinal plants such as solvent types, extraction time, extraction technique, and liquid-to-solid ratio. In this research, the extraction factors for extraction yield and cytotoxic activity of C. aeruginosa rhizome were optimized using the Box-Behnken experimental design. Effect of ethanol concentration, the ratio of liquid to solid, and extraction time for the maceration process was studied. The cytotoxic activity was determined by the brine shrimp lethality test. The optimum value that maximizes the extraction yield was 70 % ethanol, 300:15 ml/g liquid to solid ratio, and 1-day extraction time. The optimum value that maximizes the cytotoxic activity was 70 % ethanol, 150:15 ml/g liquid to solid ratio, and 2-day extraction time. The predicted extraction yield and cytotoxic activity at these projected values are 14.78 % and 78.26 mg/l, respectively. In this model, Adeq Precision (10.35 and 4.16), R-Squared (0.86 and 0.79), and F-value (7.92 and 2.04) is rational to fit the model for extraction yield and cytotoxic activity from C. aeruginosa rhizome.

scholarly journals Box-Behnken Experimental Design for Extraction of Spinosin from Ziziphus mauritiana Lam. Seeds

2019 ◽  
Vol 31 (5) ◽  
pp. 1045-1048
Author(s):  
Quang Thuong Tran ◽  
Loan Thi Thanh Vu ◽  
Tram Huyen Le ◽  
Ly Thi Phuong Giang ◽  
Toyonobu Usuki
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Factorial Design ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Raw Material ◽  
Extraction Time ◽  
Ziziphus Mauritiana ◽  
Solid Ratio ◽  
Extraction Parameters

The objective of this study was to evaluate the effects of extraction parameters (ethanol concentration, extraction ratio of solvent to raw material and extraction time) on the yield of spinosin from the seeds of Ziziphus mauritiana Lam. Box-Behnken factorial design was used in association with response surface methodology. The optimal extraction conditions were an ethanol concentration of 61 %, 40 (v/w) liquid-to-solid ratio, and 2.6 h extraction time with reflux. Spinosin was purified using Diaion HP20SS as an adsorbent. The maximum spinosin yield was 1.18 mg/g raw material. Present findings can be applied to future approaches for maximizing the extraction of spinosin from Z. mauritiana Lam. seeds.

scholarly journals Effect of Time, Solvent-Solid Ratio, Ethanol Concentration and Temperature on Extraction Yield of Phenolic Compounds From Olive Leaves

2018 ◽  
Vol 8 (2) ◽  
pp. 2805-2808
Author(s):  
N. Elboughdiri
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimal Conditions ◽  
Total Phenolic Compounds ◽  
Olive Leaves ◽  
Factors Affecting ◽  
Solid Ratio

This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Comparison of Two Protein Extraction Methods for Tea Residues

2011 ◽  
Vol 138-139 ◽  
pp. 933-936 ◽  
Author(s):  
Xuan Chen ◽  
Hong Yu Luo ◽  
Jun Yu ◽  
Peng Xiang Yue ◽  
Lin Zhou ◽  
...  
Keyword(s):  
High Temperature ◽  
Protein Extraction ◽  
Ethanol Concentration ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Factorial Experiments ◽  
Extraction Technology ◽  
Digestion Method ◽  
Solid Ratio ◽  
Lower Temperature

Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

scholarly journals Optimisation of High Hydrostatic Pressure Assisted Extraction of Anthocyanins from Rabbiteye Blueberry Pomace

2017 ◽  
Vol 35 (No. 2) ◽  
pp. 180-187 ◽  
Author(s):  
Zhang Haining ◽  
Ma Yongkun
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Anthocyanin Content ◽  
Vaccinium Ashei ◽  
Box Behnken Design ◽  
Solid Ratio ◽  
Assisted Extraction

The purpose of this study was to evaluate the influence of high hydrostatic pressure assisted extraction (HHPE) on the anthocyanins from blueberry (Vaccinium ashei) pomace. From the Plackett-Burman Experimental Design (PBD) only the liquid-solid ratio, ethanol concentration, and extraction pressure were found to significantly affect the extraction yield of anthocyanin content. Hence, the outcome of Box-Behnken Design suggested that the optimal operating conditions of the HHPE for the yield of anthocyanin content were liquid-solid ratio 41 ml/g, ethanol concentration 63%, and extraction pressure 443 MPa. At these conditions, 107.9 mg/100 g anthocyanins was obtained, which was more than by the control extraction (67.63 mg/100 g). 10 anthocyanins were identified by HPLC-ESI-MS, malvidin-3-galactoside and malvidin-3-glucoside were the major anthocyanins.

Optimization of Extraction of Bioactive Material-Polysaccharides from the Roots of Taraxacum mongolicum by Box-Behnken Experimental Design

2013 ◽  
Vol 749 ◽  
pp. 354-358
Author(s):  
Kai Chang ◽  
Guang Ren Sun ◽  
Da Di Yao
Keyword(s):  
Experimental Design ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Changbai Mountains ◽  
Extraction Temperature ◽  
Single Variable ◽  
Medical Systems ◽  
Extraction Parameters ◽  
Materials Used

Dandelion (Taraxacum spp) is an medical active materials used in many traditional and modern herbal medical systems, its polysaccharides have many bioactivities. Taraxacum mongolicum is one of the most widely consumed as Chinese herbal medicine and root tea in Changbai Mountains area, Northeast China. In this study, a procedure for the extraction of polysaccharides from the root of T mongolicum(TMRPS) was established. Based on single-variable tests experiment to determine the ranges of the extraction parameters (extraction temperature (X1), extraction time (X2), and ratio of solvent to raw material (X3)), response surface methodology (RSM) was used to optimize for these extraction parameters (X1, X2, X3) enhancing the forward extraction efficiency of TMRPS by a three-level three-variable Box-Behnken experimental design. The results showed that the ranges of the extraction parameters were determined as 60°C-80°C(X1),1h-3h (X2),20-40(v/m)(X3) by single-variable tests and the maximal yield (9.01%) of TMRPS was reached at extraction temperature 70.2°C, extraction time 1.79 h and ratio of solvent to raw material 28.0 (v):1 (m). Convenient for experiment, appropriate readjustment of these extraction parameters was made. Under these readjusted conditions, the mean extraction yield of TMRPS was 8.85%, which was near to theoretical calculation and indicates that these results were agree closely with model predictions.

scholarly journals Recycling of pomegranate peel and mandarin peel with ultrasound assisted solvent extraction

2020 ◽  
Vol 3 (1) ◽  
pp. 161-169
Author(s):  
Merve Hüseyinbaş ◽  
Berkay Korkmaz ◽  
Selin Yücelen ◽  
Afife Güvenç
Keyword(s):  
Particle Size ◽  
Extraction Yield ◽  
Green Technology ◽  
Extraction Time ◽  
Total Phenolic ◽  
Distilled Water ◽  
Pomegranate Peel ◽  
Solid Ratio ◽  
Mandarin Peel ◽  
Ultrasound Assisted

According to the data of Food and Agriculture Organization, approximately one third of the food produced in the world (1.6 billion tons/year) is lost when it is not reused at the end of consumption. For the recovery and reuse of valuable ingredients (flavonoids, anthocyanins, phenolic acids) in ‘Katırbaşı’ pomegranate peel and ‘Satsuma’ type mandarin peel, which are food industry wastes, ultrasound assisted extraction method (UAEM) which is green technology was used. The effects of various solvents and particle size on extraction yield were investigated by applying solvent / solid ratio of 5 ml/g , particle size of 0.5-1 mm pomegranate peels and 10 min. extraction time for UAEM. The highest efficiency was achieved by using water with 18%. Total phenolic content (TPC) values are 16.55 mg GAE/g dry peel(dp), 14.4 mg GAE/g dp, 12.55 mg GAE/g dp, 10.9 mg GAE/g dp, 14.45 mg GAE/g dp for distilled water, methanol, 50% methanol-water, ethanol, 70% ethanol-water solvents, respectively, for 0.5-1 mm particle size. TPC was found as 11.75 mg GAE/g dp for particle size of 0.25mm and below and 17.5 mg GAE/g dp for the range of particle size of 1-2 mm using water. Also, similar experiments were made for mandarin peels; TPC and AA (Antioxidant Activity, with DPPH) were determined. The parameters were chosen as different solvent type (water, 100% ethanol, 80% ethanol-water, 100% methanol, 80% methanol-water), extraction time (10 and 20 min.) and solvent/solid ratio (10, 20 ml/g) as well. TPC and AA were found 17.8 mg GAE/g dp and 90% for 100% methanol, 10 minutes and 20 ml/g solvent/solid ratio, respectively. When the ultrasound application time was 20 min, TPC and AA were found 18.24 mg GAE/g dp and 90% for 20 ml/g solvent/solid ratio and 80% methanol, respectively. The highest extraction yield was 19% using distilled water for 20 min, 10 ml/g solvent/solid ratio.

OPTIMIZATION OF PHYTOESTROGENS EXTRACTION FROM SOY GERM USING RESPONSE SURFACE METHODOLOGY

2021 ◽  
pp. 70-78
Author(s):  
Chau Le Minh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Extraction Time ◽  
Optimum Conditions ◽  
Potential Health ◽  
Box Behnken Design ◽  
Independent Variables ◽  
Solid Ratio

Phytoestrogens are phytochemicals with antioxidant activities and potential health benefits. Their contents in soy germ is the highest compared to that in parts of soy seed. Response surface methodology (RSM) using Box Behnken Design of four factors was employed to optimize the extraction conditions for phytoestrogens. The Box Behnken Design with five replicates at central point was applied. The four independent variables investigated in this experiment were extraction time (X1) 60-120 minutes, solvent/solid ratio (X2) 8-12, extraction pH (X3) 8-10, ethanol concentration (X4) 50-70%. The high coefficient values indicated that the variables were fitted to the regression for the total phytoestrogens (R2 = 0.9887). Optimum conditions for maximizing total crude phytoestrogen content were 90 minutes for the extraction time, 12/1 for the extraction ratio, 9 for the extraction pH, and 65%  for the ethanol concentration.

scholarly journals Ultrasound-Assisted Extraction of Taxifolin, Diosmin, and Quercetin from Abies nephrolepis (Trautv.) Maxim: Kinetic and Thermodynamic Characteristics

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1401
Author(s):  
Mengxia Wei ◽  
Ru Zhao ◽  
Xiaojin Peng ◽  
Chunte Feng ◽  
Huiyan Gu ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Ultrasound Irradiation ◽  
Optimal Operation ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Operation Conditions ◽  
Assisted Extraction ◽  
Irradiation Power ◽  
Ultrasound Assisted

Extraction behaviors of the 3 flavonoids taxifolin, diosmin, and quercetin have been investigated in Abies nephrolepis leaves and bark. The following operation parameters—ethanol volume fraction, liquid–solid ratio, temperature, ultrasound irradiation power and time, and ultrasound frequency—were varied to study their effect on the yield of the 3 flavonoids during extraction. The results showed that a low extraction efficiency occurred at 293.15 K due to slow kinetics, while the situation was significantly improved at 333.15 K. The kinetic data for the extraction yields of the 3 flavonoids achieved good fits by the first-order kinetic model. From the thermodynamic analysis results, we realized that the ultrasound-assisted extraction of taxifolin, diosmin, and quercetin from the leaves and bark of A. nephrolepis was a spontaneous and endothermic process in which the disorder increased (ΔG0 < 0, ΔH0 > 0, and ΔS0 > 0). According to the response surface methodology (RSM) analysis, under the optimal operation conditions (ethanol concentration of 50%, liquid–solid ratio of 20 mL/g, frequency of 45 kHz, extraction time of 39.25 min, ultrasound irradiation power of 160 W and temperature of 332.19 K), the total yield of the 3 flavonoids were 100.93 ± 4.01 mg/g from the leaves of A. nephrolepis (with 31.03 ± 1.51 mg/g, 0.31 ± 0.01 mg/g, 69.59 ± 2.57 mg/g for taxifolin, diosmin, and quercetin, respectively), and under the optimal operation conditions (ethanol concentration of 50%, liquid–solid ratio of 20 mL/g, frequency of 45 kHz, extraction time of 36.80 min, ultrasound irradiation power of 150 W and temperature of 328.78 K), 16.05 mg/g ± 0.38 mg/g were obtained from the bark of A. nephrolepis (with 1.44 ± 0.05 mg/g, 0.47 ± 0.01 mg/g, 14.14 ± 0.38 mg/g for taxifolin, diosmin, and quercetin, respectively), which were close to the prediction values.

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