Optimization of Extraction of Bioactive Material-Polysaccharides from the Roots of Taraxacum mongolicum by Box-Behnken Experimental Design

2013 ◽  
Vol 749 ◽  
pp. 354-358
Author(s):  
Kai Chang ◽  
Guang Ren Sun ◽  
Da Di Yao
Keyword(s):  
Experimental Design ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Changbai Mountains ◽  
Extraction Temperature ◽  
Single Variable ◽  
Medical Systems ◽  
Extraction Parameters ◽  
Materials Used

Dandelion (Taraxacum spp) is an medical active materials used in many traditional and modern herbal medical systems, its polysaccharides have many bioactivities. Taraxacum mongolicum is one of the most widely consumed as Chinese herbal medicine and root tea in Changbai Mountains area, Northeast China. In this study, a procedure for the extraction of polysaccharides from the root of T mongolicum(TMRPS) was established. Based on single-variable tests experiment to determine the ranges of the extraction parameters (extraction temperature (X1), extraction time (X2), and ratio of solvent to raw material (X3)), response surface methodology (RSM) was used to optimize for these extraction parameters (X1, X2, X3) enhancing the forward extraction efficiency of TMRPS by a three-level three-variable Box-Behnken experimental design. The results showed that the ranges of the extraction parameters were determined as 60°C-80°C(X1),1h-3h (X2),20-40(v/m)(X3) by single-variable tests and the maximal yield (9.01%) of TMRPS was reached at extraction temperature 70.2°C, extraction time 1.79 h and ratio of solvent to raw material 28.0 (v):1 (m). Convenient for experiment, appropriate readjustment of these extraction parameters was made. Under these readjusted conditions, the mean extraction yield of TMRPS was 8.85%, which was near to theoretical calculation and indicates that these results were agree closely with model predictions.

scholarly journals Box-Behnken Experimental Design for Extraction of Spinosin from Ziziphus mauritiana Lam. Seeds

2019 ◽  
Vol 31 (5) ◽  
pp. 1045-1048
Author(s):  
Quang Thuong Tran ◽  
Loan Thi Thanh Vu ◽  
Tram Huyen Le ◽  
Ly Thi Phuong Giang ◽  
Toyonobu Usuki
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Factorial Design ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Raw Material ◽  
Extraction Time ◽  
Ziziphus Mauritiana ◽  
Solid Ratio ◽  
Extraction Parameters

The objective of this study was to evaluate the effects of extraction parameters (ethanol concentration, extraction ratio of solvent to raw material and extraction time) on the yield of spinosin from the seeds of Ziziphus mauritiana Lam. Box-Behnken factorial design was used in association with response surface methodology. The optimal extraction conditions were an ethanol concentration of 61 %, 40 (v/w) liquid-to-solid ratio, and 2.6 h extraction time with reflux. Spinosin was purified using Diaion HP20SS as an adsorbent. The maximum spinosin yield was 1.18 mg/g raw material. Present findings can be applied to future approaches for maximizing the extraction of spinosin from Z. mauritiana Lam. seeds.

Optimization of Crude Polysaccharides Extraction from Plantago ovata Forsk Seed by Response Surface Methodology

2020 ◽  
Vol 16 (3) ◽  
pp. 354-363
Author(s):  
Ehsan Sadeghi ◽  
Reza Mohammadi ◽  
Mohammad H. Shirgardoun ◽  
Fatemeh Askari ◽  
Masoumeh Taghizadeh ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Food Packaging ◽  
Polynomial Equation ◽  
Edible Films ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Plantago Ovata

Background: Plantago ovata Forsk (Psyllium seed) is an annual plant of plantago genus. This plant widely grows in India and Iran. The Psyllium seed contains mucilage, protein, sugar, fat and tannins. Seed’s husk is a known source to extract hydrocolloid. Objective: The aim of this study was to optimize gum extraction from the Psyllium seed. Methods: The response surface methodology (RSM) was used to estimate the effect of independent variables on the extraction yield of Psyllium gum. The obtained experimental data were fitted to a second- order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. A central composite design (CCD) was used for experimental design and analysis of the results to obtain the best possible combination of extraction temperature (X1= 60-80°C), extraction time (X2= 1-3 h), and the ratio of water to raw material (X3= 30-70%) for the maximum gum extraction. Results: Optimum extraction conditions were extraction temperature of 79°C, extraction time of 2.5 h, and the ratio of water to the raw material of 57%. The experimental extraction yield under optimal conditions was found to be 9 ± 0.25%, which was in agreement with the predicted value of 9.4%. Conclusion: This study showed that Psyllium gum can be used as a hydrocolloid source for pharmaceutical and food industry such as edible films for food packaging.

scholarly journals Optimization of Extraction Parameters of Polyphenols from Mango Seed Kernel through Response Surface Methodology

2020 ◽  
Vol 5 (8) ◽  
pp. 877-883
Author(s):  
Junior Franck Ekorong Akouan Anta ◽  
Dorothée Marcelle Biloa ◽  
K.S.M.S. Raghavarao
Keyword(s):  
Phenolic Compounds ◽  
Reducing Power ◽  
Extraction Yield ◽  
Agitation Speed ◽  
Extraction Time ◽  
Seed Kernel ◽  
Extraction Temperature ◽  
Mango Seed Kernel ◽  
Extraction Parameters ◽  
Mango Seed

Phenolic compounds constitute an essential part of the human diet, and are of considerable interest due to their antioxidant properties. The traditional maceration method has been used for the extraction of polyphenols from mango seed kernel (Mangifera indica L.). Highlight the effects of different extraction parameters is useful to optimize the process, as well as to predict the extraction yield within the experimental domain with enough precision and confidence. The purpose of this work is to probe the influence of extraction time, extraction temperature and agitation speed on the extraction yield of phenolic compounds, and the total reducing power of the extract. The Surface Methodology (RSM), using the Doehlert design, have been applied. Optimal values of extraction yield and total reducing power was 36.99 mg Gallic acid equivalent/g and 61.08 mg Ascorbic Acid Equivalent/g respectively. The optimal conditions are 60 minutes of extraction time, 68.7°C extraction temperature and 424 rpm for agitation speed. Under optimized conditions the experimental values well agreed with the values predicted by the model equations proposed.

scholarly journals Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

2021 ◽  
Vol 3 (4) ◽  
Author(s):  
Jirapornchai Suksaeree ◽  
Natawat Chankana ◽  
Chitradee Luprasong ◽  
Chaowalit Monton
Keyword(s):  
Optimal Condition ◽  
Computer Software ◽  
Hplc Method ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Time ◽  
Percentage Error ◽  
Extraction Temperature ◽  
Central Composite Experimental Design ◽  
Plant Raw Material

AbstractTrans-anethole possesses several biological and pharmacological effects. It is also used as masking agent in household products and as a flavoring agent in food. Clausena anisata (Willd.) Hook. f. ex Benth. leaves are reported as a source of trans-anethole that makes their anise-like odor. This work sought to optimize the dynamic maceration of C. anisata leaves to maximize the trans-anethole content. The circumscribed central composite experimental design was applied to investigate the effects of extraction temperature and time on extraction yield, trans-anethole content in the extract, and trans-anethole content in the plant raw material. The results showed that the extraction yield was high when C. anisata leaves were extracted over a long extraction time, while the extraction temperature had a lesser effect on the extraction yield. Trans-anethole content in the extract and plant raw material determined using a linear, specific, precise, and accurate HPLC method was high when a medium extraction temperature with a short extraction time was used. The optimal condition that maximized trans-anethole content involved an extraction temperature of 61.8 °C and an extraction time of 12.9 min, respectively. The percentage error of the prediction conducted by computer software was low, suggesting that the prediction was highly accurate. In conclusion, the optimal condition of dynamic maceration obtained from this work could be used as a guide for maximizing trans-anethole content from C. anisata leaves.

scholarly journals Polyphenols From Vitis vinifera Lambrusco By-Products (Leaves From Pruning): Extraction Parameters Evaluation Through Design of Experiment

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290 ◽  
Author(s):  
Massimo Tacchini ◽  
Ilaria Burlini ◽  
Immacolata Maresca ◽  
Alessandro Grandini ◽  
Tatiana Bernardi ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Antioxidant Capacity ◽  
Design Of Experiment ◽  
Extraction Yield ◽  
Wine Industry ◽  
Raw Material ◽  
Extraction Time ◽  
Total Phenolic ◽  
Vitis Vinifera L ◽  
By Products

Vitis vinifera L. leaves from pruning are by-products of the wine industry and represent an important source of secondary raw material, thanks to their polyphenols content. Optimization of the extraction processes is a key factor for their valorization, and Design of Experiment (DOE) could be a tool to obtain the most performing extract in terms of polyphenols quality/quantity and bioactivity. Vitis vinifera Lambrusco leaves were subjected to ultrasound-assisted extractions guided by a 23 factorial design. Three independent parameters (% solvent, time of extraction, and solvent:solid ratio) were considered to evaluate the extraction process by analyzing the extraction yield, the total phenolic content (Folin-Ciocalteu assay), and the antioxidant capacity (DPPH assay). Moreover, the content of the main molecules was identified and quantified by reversed-phase high-performance liquid chromatography coupled with diode array detection and mass spectrometry. The DOE highlighted the best extraction conditions that showed slight changes considering the different evaluating parameters. The highest extraction yield was obtained by extraction with 100% water, 60 minutes of extraction time, and 30:1 solvent:solid ratio, but it was neither the richest in polyphenols nor antioxidant capacity. The latter 2 characteristics were associated with the extraction performed using 50% ethanol, 35 minutes of extraction time, and a 20:1 solvent:solid ratio. That extract also exhibited the highest quantity of flavonols.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Optimization of the Ultrasonic Extraction of Gypenoside III from Gynostemma pentaphyllum by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1866-1870
Author(s):  
Xiao Dan Tang ◽  
Hai Yang Hang ◽  
Shao Yan Wang ◽  
Jing Xiang Cong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Gynostemma Pentaphyllum ◽  
Bioactive Component ◽  
Yield Value ◽  
Ultrasonic Time

Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.

scholarly journals Optimization of Supercritical Carbon Dioxide Extraction of Quercus infectoria Oil

2015 ◽  
Vol 74 (7) ◽  
Author(s):  
Liza Md Salleh ◽  
Stashia ELeaness Rosland Abel ◽  
Gholamreza Zahedi ◽  
Russly Abd Rahman ◽  
Hasmida Mohd Nasir ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Goodness Of Fit ◽  
Maximum Yield ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Predicted Values ◽  
Artificial Neural Network Ann ◽  
Quercus Infectoria

This current study focuses on the modelling and optimization of supercritical fluid extraction of Quercus infectoria galls oil. In this case, response surface methodology (RSM) and artificial neural network (ANN) were applied for the modelling and prediction of extraction yield of galls oil. A 17-run Box-Behnken Design (BBD) was employed to statistically optimize the process parameters of SC-CO2 extraction of Quercus infectoria galls at a condition as follows: pressure (5000, 6000, 7000 Psi), temperature (40, 50, 60°C) and extraction time (30, 45, 60 min). The maximum yield of the extracted oil is1.12 % and the optimum conditions are at an extraction pressure of 5574 Psi; extraction temperature of 75°C and extraction time of 54 min. Under the optimal conditions, the experimental results agree with the predicted values obtained through analysis of variance (ANOVA). This indicates a successful response surface methodology and highly satisfactory goodness of fit of the model used. The analysis of experimental design for process optimization results demonstrates that temperature and extraction time are the main parameters that influence the oil extraction of Quercus infectoria.

scholarly journals Extraction of Illegal Dyes from Red Chili Peppers with Cholinium-Based Deep Eutectic Solvents

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Shuqiang Zhu ◽  
Dongling Liu ◽  
Xinyue Zhu ◽  
Along Su ◽  
Haixia Zhang
Keyword(s):  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Raw Material ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Green Solvents ◽  
Hydrogen Bond Donor ◽  
Relative Standard ◽  
Orange G

Deep eutectic solvents (DESs) as a new kind of green solvents have been used to extract bioactive compounds but there are few applications in extracting chrysoidine dyes. In this study, we developed an ultrasonic-assisted extraction method with choline chloride/hydrogen bond donor (ChCl/HBD) DES for the extraction of chrysoidine G (COG), astrazon orange G (AOG), and astrazon orange R (AOR) in food samples. Some experimental parameters, such as extraction time, raw material/solvent ratio, and temperature, were evaluated and optimized as follows: the ratio of ChCl/HBD, 1 : 2 (v/v); the ratio of sample/DES, 1 : 10 (g/mL); extraction time, 20 min; extraction temperature, 50°C. Under the optimized conditions, the limits of detection (μg/mL) were 0.10 for COG and 0.06 for AOG and AOR. The relative standard deviations were in the range of 1.2–2.1%. The recoveries of the three dyes were in the range of 80.2–105.0%. By comparing with other commonly used solvents for extracting chrysoidine dyes, the advantages of DESs proved them to be potential extraction solvents for chrysoidine G, astrazon orange G, and astrazon orange R in foods.

scholarly journals OPTIMIZATION CONDITION EXTRACTION USED ENZYME WITH RESPONSE SURFACE METHODOLOGY TO EXTRACT ROSELLE PETALS (Hibiscus sabdariffa L.)

2019 ◽  
Vol 9 (2) ◽  
Author(s):  
Mardiah Mardiah ◽  
Rima Nidaul Hasanah ◽  
Noli Novidahlia ◽  
A.E. Zainal Hasan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Raw Material ◽  
Extraction Time ◽  
Anthocyanin Content ◽  
Extraction Temperature ◽  
Hibiscus Sabdariffa ◽  
Cellulase Enzymes ◽  
Yield Level

Roselle petals can be made into extracts as a source of raw material for food products and other products. Previous research states that the use of pectinase and cellulase enzymes can increase the yield including the anthocyanin content. This study aims to optimize the extraction conditions of roselle flower petals  (Hibiscus sabdariffa L.) using pectinase and cellulase enzymes by using Response Surface Methodology (RSM). The treatment conditions were using pectinase and cellulase enzyme (1: 1) with concentrations in the range between 500-1500 ppm, extraction temperature between 35-65 oC and extraction time between 30-90 minutes. Analysis of the best treatment was carried out on the yield level, anthocyanin content and total dissolved solids tested using central composite design, obtained R2 values for the three responses of 0.6. The results showed that based on the surface response, the optimum conditions of the best  extraction of the rosella at the addition of enzyme concentration of 1000 ppm, extraction temperature of 50 oC and extraction time of 10 minutes. In this condition, the yield of dried extracts of rosella was 84,20%, anthocyanins content 479,70 mg/L and total soluble solid 2,13 oBrix.

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