scholarly journals Effect of Time, Solvent-Solid Ratio, Ethanol Concentration and Temperature on Extraction Yield of Phenolic Compounds From Olive Leaves

2018 ◽  
Vol 8 (2) ◽  
pp. 2805-2808
Author(s):  
N. Elboughdiri
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Optimal Conditions ◽  
Total Phenolic Compounds ◽  
Olive Leaves ◽  
Factors Affecting ◽  
Solid Ratio

This study aims to determine the factors affecting the process of extraction of phenolic compounds from olive leaves. Two methods of extraction were used in this work and different tests were implemented with the aim of optimizing the recovery of phenolic compounds from olive leaves. The factors considered were the extraction time, temperature, solvent-solid ratio and the ethanol concentration. Obtained results show that the recommended optimal conditions for the extraction of the total phenolic compounds from olive leaves were found at 40°C with a solvent/solid ratio of 30:1 and ethanol concentration of 80% (v/v) when the dried olive leaves are stored at -21°C at the beginning of the experiments.

scholarly journals Extraction of Polyphenols from Olive Leaves Employing Deep Eutectic Solvents: The Application of Chemometrics to a Quantitative Study on Antioxidant Compounds

2022 ◽  
Vol 12 (2) ◽  
pp. 831
Author(s):  
Hamida Akli ◽  
Spyros Grigorakis ◽  
Abdessamie Kellil ◽  
Sofia Loupassaki ◽  
Dimitris P. Makris ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Deep Eutectic Solvents ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Olive Leaves ◽  
Box Behnken Design ◽  
Solid Ratio

The extraction of phenolic compounds from olive leaves was optimized using three glycerol-based deep eutectic solvents (DESs) with lysine, proline, and arginine. A three-level Box–Behnken design was used to examine the influence of the liquid/solid ratio, concentration of DESs, and extraction temperature on the yield of the extraction process. A second-order polynomial model was used for predicting the polyphenol extraction yield. The optimal predicted conditions were used for extractions and they provided the highest total phenol yields with the glycerol–lysine exhibiting the best performance. Quantification of tyrosol, hydroxytyrosol, oleuropein, luteolin-7-O-glucoside, and rutin in the extracts showed high content in tyrosol in all DESs, particularly with glycerol–lysine and relatively similar contents with other studies for the other phenolic compounds. Finally, a linear relationship between tyrosol content and the total phenolic content of the extracts was observed.

scholarly journals Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaeaL.)

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pu-jun Xie ◽  
Li-xin Huang ◽  
Cai-hong Zhang ◽  
Feng You ◽  
Cheng-zhang Wang ◽  
...  
Keyword(s):  
Ethanol Concentration ◽  
Extraction Yield ◽  
Optimal Operation ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Olive Leaves ◽  
Sem Images ◽  
Reduced Pressure ◽  
Operation Conditions

Oleuropein was extracted fromFrantoioolive leaves using reduced-pressure boiling extraction coupled with ultrasound-assist (URPE). Four important factors, extraction temperature, ultrasonic power, ethanol concentration, and the ratio of solid to liquid, were selected to carry out the response surface methodology (RSM) for seeking optimal conditions on high oleuropein extraction yield at different levels. Box-Behnken design was employed to investigate the effects of the four factors on it. The results showed that the ratio of solid to liquid was the most significant factor of all on oleuropein yield. The optimal operation conditions were obtained as follows: ethanol concentration 75% (v/v), extraction temperature 53°C, ultrasonic power 600 W, and the ratio of solid to liquid 1 : 31. Under these optimal conditions, oleuropein extraction yield was 7.08%, which was close to the predicted value 7.121%. The scanning electron microscope (SEM) images of olive leaves after extraction were provided as well. It was seen that, compared with the untreated leaves, URPE could effectively break cells within the olive leaves.

scholarly journals Recycling of pomegranate peel and mandarin peel with ultrasound assisted solvent extraction

2020 ◽  
Vol 3 (1) ◽  
pp. 161-169
Author(s):  
Merve Hüseyinbaş ◽  
Berkay Korkmaz ◽  
Selin Yücelen ◽  
Afife Güvenç
Keyword(s):  
Particle Size ◽  
Extraction Yield ◽  
Green Technology ◽  
Extraction Time ◽  
Total Phenolic ◽  
Distilled Water ◽  
Pomegranate Peel ◽  
Solid Ratio ◽  
Mandarin Peel ◽  
Ultrasound Assisted

According to the data of Food and Agriculture Organization, approximately one third of the food produced in the world (1.6 billion tons/year) is lost when it is not reused at the end of consumption. For the recovery and reuse of valuable ingredients (flavonoids, anthocyanins, phenolic acids) in ‘Katırbaşı’ pomegranate peel and ‘Satsuma’ type mandarin peel, which are food industry wastes, ultrasound assisted extraction method (UAEM) which is green technology was used. The effects of various solvents and particle size on extraction yield were investigated by applying solvent / solid ratio of 5 ml/g , particle size of 0.5-1 mm pomegranate peels and 10 min. extraction time for UAEM. The highest efficiency was achieved by using water with 18%. Total phenolic content (TPC) values are 16.55 mg GAE/g dry peel(dp), 14.4 mg GAE/g dp, 12.55 mg GAE/g dp, 10.9 mg GAE/g dp, 14.45 mg GAE/g dp for distilled water, methanol, 50% methanol-water, ethanol, 70% ethanol-water solvents, respectively, for 0.5-1 mm particle size. TPC was found as 11.75 mg GAE/g dp for particle size of 0.25mm and below and 17.5 mg GAE/g dp for the range of particle size of 1-2 mm using water. Also, similar experiments were made for mandarin peels; TPC and AA (Antioxidant Activity, with DPPH) were determined. The parameters were chosen as different solvent type (water, 100% ethanol, 80% ethanol-water, 100% methanol, 80% methanol-water), extraction time (10 and 20 min.) and solvent/solid ratio (10, 20 ml/g) as well. TPC and AA were found 17.8 mg GAE/g dp and 90% for 100% methanol, 10 minutes and 20 ml/g solvent/solid ratio, respectively. When the ultrasound application time was 20 min, TPC and AA were found 18.24 mg GAE/g dp and 90% for 20 ml/g solvent/solid ratio and 80% methanol, respectively. The highest extraction yield was 19% using distilled water for 20 min, 10 ml/g solvent/solid ratio.

scholarly journals Ultrasound-Assisted Extraction of Total Phenolic Compounds fromInula helenium

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Jin Wang ◽  
Yong-Ming Zhao ◽  
Ya-Ting Tian ◽  
Chun-Lin Yan ◽  
Chun-Yan Guo
Keyword(s):  
Phenolic Compounds ◽  
Ethanol Concentration ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Liquid Ratio ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Time ◽  
Ultrasound Assisted ◽  
Solid Liquid

Ultrasound-assisted extraction (UAE) of phenolic compounds fromInula heleniumwas studied. Effects of ethanol concentration, ultrasonic time, solid-liquid ratio, and number of extractions were investigated. An orthogonal array was constructed to optimize UAE process. The optimized extraction conditions were as follows: ethanol concentration, 30%; solid-liquid ratio, 1 : 20; number of extractions, 2 times; extraction time, 30 min. Under the optimal conditions, the yield of total phenolic compounds and chlorogenic acid was6.13±0.58and1.32±0.17 mg/g, respectively. The results showed that high amounts of phenolic compounds can be extracted fromI. heleniumby ultrasound-assisted extraction technology.

scholarly journals Ekstraksi Senyawa Fenolik Daun Kenikir (Cosmos caudatus) menggunakan Microwave Assisted Extraction (MAE)

2018 ◽  
Vol 11 (1) ◽  
pp. 59-70
Author(s):  
Angky Wahyu Putranto ◽  
Shinta Rosalia Dewi ◽  
Ni'matul Izza ◽  
Dian Rahmat Yuneri ◽  
Maria Yeniaska S. Dachi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Microwave Assisted ◽  
Cosmos Caudatus ◽  
Assisted Extraction

Abstrak. Kenikir (Cosmos caudatus) adalah tanaman yang sering dimanfaatkan daunnya untuk bahan pangan maupun juga sebagai obat-obatan karena memiliki kandungan senyawa fenolik yang cukup tinggi. Ekstraksi senyawa fenol menggunakan metode konvensional masih memerlukan waktu yang cukup lama dan jumlah pelarut yang banyak. Sehingga perlu dicari alternatif metode ekstraksi yang memiliki waktu ekstraksi dan kebutuhan pelarut yang relatif rendah seperti menggunakan Microwave Assisted Extraction (MAE). Tujuan dari penelitian ini adalah melakukan ekstraksi senyawa fenolik dari daun kenikir dengan metode MAE terhadap total fenol dan aktivitas antioksidan. Penelitian ini menggunakan 2 faktor perlakuan yaitu rasio pelarut terhadap kenikir segar yaitu 1:4, 1:6, dan 1:8 (b/v) dan waktu ekstraksi 2, 3 dan 4 menit. Penelitian ini menjelaskan bahwa nilai total fenol tertinggi (17,162 ± 0,11 mg GAE/g berat segar) diperoleh pada perlakuan rasio pelarut 1:8 (b/v), sedangkan aktivitas antioksidan tertinggi (4,203 ± 0,26 mg/ml) pada perlakuan rasio pelarut 1:4 (b/v). Waktu perlakuan 3 menit menunjukkan hasil yang optimal untuk nilai total fenol dan aktivitas antioksidan. Hasil tersebut juga menunjukkan nilai yang lebih baik jika dibandingkan dengan metode konvensional baik dengan maserasi biasa dan maserasi dengan waterbath. Extraction of Phenolic Compounds from Cosmos caudatus using Microwave Assisted Extraction (MAE) Abstract. Cosmos caudatus, a plant commonly used its leaves for food and also as a medicine since it contains high phenolic compounds. The extraction of phenolic compounds using conventional methods still require more extraction time and need much more of solvent. Therefore, it was necessary to find an alternative extraction method that has a rapid extraction time and a few solvent need such as using Microwave Assisted Extraction (MAE). The aim of this study is to extract of phenolic compounds from Cosmos caudatus with MAE method of total phenol and antioxidant activity. This study using 2 treatment factors, the sampel-solvent ratio of 1:4, 1:6, and 1:8 (w/v) and the extraction time of 2, 3 and 4 minutes. This study explained that the highest total phenolic compounds (17.162 ± 0.11 mg GAE/g fw) and antioxidant activity (4.203 ± 0.26 mg/ml) were obtained at 1:8 and 1:4 (w/v) of sampel-solvent ratio, respectively. The extraction time of 3 minutes was the optimal condition for total phenolic compounds and antioxidant activity. These results also showed the highest parameter value when compared with both conventional methods: maceration and maceration with waterbath. 

scholarly journals Box-Behnken experimental design for extraction optimization of cytotoxic activity from Curcuma aeruginosa rhizome

2020 ◽  
Vol 11 (2) ◽  
pp. 1631-1637
Author(s):  
Rani Khoiriyah ◽  
Made Artika I ◽  
Waras Nurcholis
Keyword(s):  
Experimental Design ◽  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Pharmacological Activities ◽  
Solid Ratio ◽  
Bioactive Content ◽  
The Common

Curcuma aeruginosa is the common name of temuhitam in Indonesia, and the rhizome of parts plant had several pharmacological activities. Generally, pharmacological activities were associated with bioactive content in the extract of medicinal plants. Several factors can influence the bioactive extraction from medicinal plants such as solvent types, extraction time, extraction technique, and liquid-to-solid ratio. In this research, the extraction factors for extraction yield and cytotoxic activity of C. aeruginosa rhizome were optimized using the Box-Behnken experimental design. Effect of ethanol concentration, the ratio of liquid to solid, and extraction time for the maceration process was studied. The cytotoxic activity was determined by the brine shrimp lethality test. The optimum value that maximizes the extraction yield was 70 % ethanol, 300:15 ml/g liquid to solid ratio, and 1-day extraction time. The optimum value that maximizes the cytotoxic activity was 70 % ethanol, 150:15 ml/g liquid to solid ratio, and 2-day extraction time. The predicted extraction yield and cytotoxic activity at these projected values are 14.78 % and 78.26 mg/l, respectively. In this model, Adeq Precision (10.35 and 4.16), R-Squared (0.86 and 0.79), and F-value (7.92 and 2.04) is rational to fit the model for extraction yield and cytotoxic activity from C. aeruginosa rhizome.

scholarly journals Extraction of Antioxidant Compounds and Pigments from Spirulina (Arthrospira platensis) Assisted by Pulsed Electric Fields and the Binary Mixture of Organic Solvents and Water

2021 ◽  
Vol 11 (16) ◽  
pp. 7629
Author(s):  
Francisco J. Martí-Quijal ◽  
Francesc Ramon-Mascarell ◽  
Noelia Pallarés ◽  
Emilia Ferrer ◽  
Houda Berrada ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Binary Mixture ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Total Phenolic Compounds ◽  
Extraction Technology ◽  
Pre Treatment

The application of pulsed electric fields (PEF) is an innovative extraction technology promoting cell membrane electroporation, thus allowing for an efficient recovery, from an energy point of view, of antioxidant compounds (chlorophylls, carotenoids, total phenolic compounds, etc.) from microalgae. Due to its selectivity and high extraction yield, the effects of PEF pre-treatment (3 kV/cm, 100 kJ/kg) combined with supplementary extraction at different times (5–180 min) and with different solvents (ethanol (EtOH)/H2O, 50:50, v/v; dimethyl sulfoxide (DMSO)/H2O, 50:50, v/v) were evaluated in order to obtain the optimal conditions for the extraction of different antioxidant compounds and pigments. In addition, the results obtained were compared with those of a conventional treatment (without PEF pre-treatment but with constant shaking). After carrying out the different experiments, the best extraction conditions to recover the different compounds were obtained after applying PEF pre-treatment combined with the binary mixture EtOH/H2O, 50:50, v/v, for 60–120 min. PEF extraction was more efficient throughout the study, especially at short extraction times (5–15 min). In this sense, recovery of 55–60%, 85–90%, and 60–70% was obtained for chlorophylls, carotenoids, and total phenolic compounds, respectively, compared to the maximum total extracted amount. These results show that PEF improves the extraction yield of antioxidant bioactive compounds from microalgae and is a promising technology due to its profitability and environmental sustainability.

scholarly journals Solid-Liquid Extraction Kinetics of Total Phenolic Compounds (TPC) from Red Dates

2018 ◽  
Vol 152 ◽  
pp. 01001 ◽  
Author(s):  
Chua Bee Lin ◽  
Chen Yen Leng
Keyword(s):  
Crude Extract ◽  
Extraction Time ◽  
Total Phenolic ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Extraction Kinetics ◽  
Solid Ratio ◽  
Extract Yield ◽  
Kinetics Of

Red dates are one of the most famous herbal plants in making traditional Chinese medicine. They contain large amount of bioactive compounds. The objectives of this research were to optimise the crude extract yield and total phenolic compounds (TPC) yield from red dates using response surface methodology (RSM) and model the extraction kinetics of TPC yield from red dates. Date fruits were dried in an oven under temperatures 50°C, 60°C, 70°C and 80°C until a constant weight was obtained. The optimum drying temperature was 60°C as it gave the highest crude extract yield and TPC yield. Besides that, single factor experiments were used to determine the optimum range of four extraction parameters which were: liquid-solid ratio (10-30 ml/g); ultrasonic power (70-90%); extraction temperature (50-70°C); and extraction time (40-60min). The optimum range of the four parameters were further optimised using the Box-Behken Design (BBD) of RSM. The extraction conditions that gave the highest crude extract yield and TPC yield were chosen. The optimum value for liquid-solid ratio, ultrasonic power, extraction temperature and extraction time were 30ml/g, 70%, 60°C and 60 min respectively. The two equations generated from RSM were reliable and can be used to predict the crude extract yield and TPC yield. The higher the extraction temperature, liquid-solid ratio, and extraction time and lower ultrasonic power, the higher the crude extract and TPC yield. Finally, the results of TPC yield versus time based on the optimum extraction parameters from RSM optimisation were fitted into three extraction kinetic models (Peleg’s model, Page’s model and Ponomaryov’s model). It was found that the most suitable kinetic model to represent the extraction process of TPC from red dates was Page’s model due to its coefficient of determination (R2) was the closest to unity, 0.9663 while its root mean square error (RMSE) was the closest to zero, 0.001534.

scholarly journals Reflux Extraction Optimization and Antioxidant Activity of Phenolic Compounds from Pleioblastus amarus (Keng) Shell

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 362
Author(s):  
Yuan Ma ◽  
Ailian Meng ◽  
Ping Liu ◽  
Yuanyuan Chen ◽  
Anqi Yuan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Composition Analysis ◽  
Extraction Temperature ◽  
Factors Affecting ◽  
Solid Ratio ◽  
Ic50 Values ◽  
Phenol Composition

Phenols were extracted from the Pleioblastus amarus (Keng) shell (PAS) using ethanol. A Plackett–Burman assessment indicated that the factors affecting polyphenol extraction included the ethanol concentration, extraction temperature, liquid to solid ratio, extraction time, and reflux extraction times; the best extraction parameters were the ethanol concentration of 75%, a 20:1 liquid to solid ratio, and an extraction time of 2.1 h. The number of polyphenols was 7.216 mg/g. Furthermore, the phenol composition analysis showed the presence of p-Coumaric acid (196.88 mg /mL) and rutin (312.9 mg /mL), which were used for the in vitro extraction and determination of the antioxidant activity. According to the A, B, C, and D antioxidant activity assays, the ethyl acetate phase was the strongest with low IC50 values of 0.169 ± 0.01 mg/mL, 0.289 ± 0.01 mg/mL, 0.372 ± 0.01 mg/mL, and 1.029 ± 0.03 mg/mL, respectively, confirming high antioxidant activity. For the n-butanol and petroleum ether phases, antioxidant activity was lower. This study showed that the polyphenol extract from Pleioblastus amarus (Keng) shell displayed excellent antioxidant activity, enhancing its practical application.

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