scholarly journals Evaluation of the analgesic activity of ethanolic extract of Populus deltoides leaves in mice

2020 ◽  
Vol 11 (4) ◽  
pp. 5942-5947
Author(s):  
Vijay Bahadur Maurya ◽  
Vishnu Prasad Yadav ◽  
Vinay Kumar
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Populus Deltoides ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Scientific Basis ◽  
Traditional Use ◽  
Writhing Test ◽  
Peripheral Mechanism ◽  
Analgesic Activities

The ethanolic leaves extract of Populus deltoides was tested for the presence of various phytoconstituents and designed to evaluate the analgesic activity in mice. The peripheral analgesic activity of ethanolic leaves extract of P.deltoides (250 and 500 mg/kg) was studied by using acetic acid stimulated writhing test and central analgesic activity of P.deltoides was studied by using hotplate process. The ethanolic leaves extract of P.deltoides  professed the existence of a variety of chemical constituents like alkaloids, saponins, flavonoids, terpenes and steroids. Leaves extract of P.deltoides appreciably decreased the writhing actions in acetic acid-induced writhing test in mice and amplified the respond time in hotplate test. These results suggest that the extract may have NSAIDs like activity through the peripheral mechanism and central analgesic activities via opioid receptors. From our study, we endowed that leaves extract of P.deltoides has feasible to analgesic activity. This study reveals that it can be used in the management of pain and provide a scientific basis for its traditional use. 

Anti-diarrheal, Analgesic and Anti-microbial activities of the plant Lalmesta (Hibiscus sabdariffa): A review

2012 ◽  
Vol 1 (3) ◽  
Author(s):  
Md Raihan Sarkar ◽  
SM Moazzem Hossen ◽  
Md Sariful Islam Howlader ◽  
Md Ashfaqure Rahman ◽  
Avijit Dey
Keyword(s):  
Antimicrobial Activity ◽  
Body Weight ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Castor Oil ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Hibiscus Sabdariffa ◽  
Phytochemical Screening ◽  
Writhing Test

The aim of this study was to phytochemically investigate the ethanolic extract of Hibiscus sabdariffa Linn. calyces and to evaluate the analgesic, anti-microbial and anti-diarrheal activities of this. The calyces of Hibiscus sabdariffa were separated from the other plant parts and sun dried and extracted using ethanol and phytochemically and pharmacologically evaluated.Different Phytochemical tests were performed for phytochemical screening and for determining the functional groups. Different methods like Disc diffusion method for antimicrobial activity determination, Castor oil induced diarrhea in mice method for the antidiarrheal study and Acetic acid induced writhing test for analgesic activity were employed. Phytochemical screening of the calyces of Hibiscus sabdariffa ensured the presence of alkaloid, flavonoids, saponins, tannins in the crude ethanolic extract. The peripheral analgesic activity was evaluated by acetic acid induced Writhing method. The extract produced 66.85% (p< 0.001) inhibition of writhing in mice at the dose of 500-mg/kg body weight, which is comparable to diclofenac sodium (78.45% (p<0.001) at the dose of 25mg/kg). The anti-diarrheal activity of the crude extract of Hibiscus sabdariffa was evaluated using the model of castor oil induced diarrhea in mice. The crude etanolic extract of Hibiscus sabdariffa (calyces) showed a marked antidiarrhoeal activity at dose of 500 mg/kg-body weight as compared to the standard antidiarrhoeal agent loperamide (dose:50mg/kg-body weight). Hibiscus sabdariffa caused an increase in latent period i.e. delayed the onset of diarrhoeal episode and decreased the frequency of defecation. Anti-microbial activity was tested using a number of micro-organisms. The peripheral analgesic activity of the ethanolic extract of the calyces of Hibiscus sabdariffa against acute inflammatory pain was significantly high as compared to potent inhibitory activity of Diclofenac (25mg/kg). Therefore, it is likely that the ethanolic extract at a dose of 400mg/kg might suppress the formation of these substances or antagonize the action of these substances and thus exerts its analgesic activity in acetic acid-induced writhing test. Anti-diarrheal activity was present in the ethanolic extract (500mg/kg) which indicate that the drug in decreased intestinal motility. This plant sample didn’t show any antimicrobial activity. DOI: http://dx.doi.org/10.3329/ijpls.v1i3.12978 International Journal of Pharmaceutical and Life Sciences Vol.1(3) 2012

scholarly journals Scientific Approaches in Screening the Analgesic property of Sida Cordata

2021 ◽  
Vol 23 (09) ◽  
pp. 1252-1262
Author(s):  
Srinivasan K ◽  
◽  
Sivakumar T ◽  
Kiruthiga N ◽  
Haja Sherief S ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Vehicle Control ◽  
Laboratory Animals ◽  
Hot Plate ◽  
Plate Method ◽  
Writhing Test ◽  
Whole Plant ◽  
Indian Medicine

Pain is likely the most common symptomatic complaint in medicine; an understanding of its mechanism is critical to interpret it. Nociception refers to the detection of noxious stimuli by nociceptors, followed by transduction and transmission of the sensory nervous information from the periphery to the brain. Plants have played a unique holistic role for the provision of food, drugs, clothing, shelter, etc. Natural compounds have been extensively explored for new drug discoveries. Indeed, plants have been used as medicines for more than 5000 years, as a source of antibiotics, antineoplastic, analgesics, cardioprotective, among others. About 70–90% of the population in developing countries continue to use ancient medicines based on plant extracts. Sida cordata popularly known as “bala” is regarded as a valuable drug in the Ayurvedic System of Indian Medicine. In order to validate the ethnomedical claim of Sida cordata as pain reliever, a study was conducted to evaluate the analgesic activity of ethanolic extract of Sida cordata whole plant in laboratory animals. For centrally mediated analgesic activity, eddy’s hot plate method was employed and Pentazocine was uses as reference control. For peripherally mediated analgesic activity, acetic acid induced writhing model was employed and Dicofenac was used as reference control. Sida cordata ethanolic extract was administered orally at the doses of 100 and 200 mg/kg. In eddy’s hot plate method, both the doses of Sida cordata significantly increased the reaction time compare to vehicle control. In acetic acid induced writhing test, both the doses of Sida cordata significantly reduced the number of writhing compare to vehicle control. In both the models, the effect produced by Sida cordata was comparable to that of respective reference control. The results obtained suggest that the ethanolic extract of Sida cordata has showed marked analgesic activity in experimental animal models and this strappingly supports the ethnopharmacological applications of the plant for the target activity.

scholarly journals A Study of Analgesic and Antimicrobial Potential of Mitragyna Parvifolia

2009 ◽  
Vol 1 (01) ◽  
Author(s):  
Kaushik D. ◽  
Khokra S. L. ◽  
Khokra S. L. ◽  
Kaushik P. ◽  
Saneja A. ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Hot Plate ◽  
Standard Drug ◽  
Writhing Test ◽  
E Coli

The present study was designed to evaluate the analgesic and antimicrobial activities of ethanolic extract of leaves of Mitragyna parvifolia plant (Rubiaceae) family. The analgesic activity was carried out on swiss albino male mice by Eddy’s hot plate and Acetic acid induced writhing test. The extract showed only moderate analgesic potential in acetic acid induced writhing test at all the test doses while the extract at the dose of 500 mg/kg (P less than 0.01) showed strong analgesic activity comparable to standard drug Diclofenac sodium (50 mg/Kg, i.p.) in hot plate method. The extract in different concentrations was also tested for antibacterial activity using agar well diffusion method. The extract significantly inhibited S. aureus and showed some degree of inhibition against P. aeruginosa and E. coli.

scholarly journals In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Shahriar Ahmed ◽  
Ahmed Rakib ◽  
Md. Ashiqul Islam ◽  
Bibi Humayra Khanam ◽  
Farhana Binta Faiz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Acetic Acid ◽  
Molecular Docking ◽  
Analgesic Activity ◽  
In Silico ◽  
Antineoplastic Activity ◽  
Paw Edema ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Abstract Background The study was conducted to evaluate the analgesic, anti-inflammatory, antipyretic properties of ethanolic extract of Tacca integrifolia rhizome (EETI) in mice and cytotoxic effects in brine shrimp nauplii followed by a PASS prediction study for some isolated compounds of T. integrifolia. Additionally, this experiment included the in silico molecular docking and ADME/T property analyses of some phytochemicals. Methods Formalin- induced paw licking test and acetic acid-induced writhing test for analgesic activity, carrageenan-induced paw edema test for anti-inflammatory potential and Brewer’s yeast-induced pyrexia test for antipyretic activity were applied. Antinociceptive and antineoplastic activity for breast cancer were revealed with PASS program. Schrodinger suite 2015 was used to evaluate the binding interaction and ADME/T properties of selected phytoconstituents with estrogen receptor alpha. Results In formalin-induced paw licking test, EETI at the doses of 200 and 400 mg/kg BW showed highly significant inhibition of writhing in both neurogenic and inflammatory phases. While EETI also exhibited highly significant, compared to control, writhing inhibition for both the doses in acetic acid-induced writhing test. Moderate anti-inflammatory effect at a dose of 400 mg/kg BW was noticed in paw-edema test. It also showed 77.51% of maximum antipyretic effect which was significantly effective compared to standard drug paracetamol (150 mg/kg) in Brewer’s yeast-induced pyrexia test. The EETI showed potential cytotoxic activity with LC50 value of 114.46 μg/mL. The PASS prediction revealed the potential antinociceptive and antineoplastic activity of target compounds. The compounds betulinic acid, quercetin-3-α-arabinoside, catsanogenin were found to be effective in molecular docking study. Conclusion It is evident that the EETI possesses highly significant analgesic activity with remarkable anti-inflammatory and antipyretic activity. The phytoconstituents have potential antinociceptive and antineoplastic (breast cancer) activity. Graphical abstract

scholarly journals A Original Article ANALGESIC ACTIVITY OF BARK AND LEAVES OF FICUS RELIGIOSA L. FROM NEPAL

2020 ◽  
pp. 32-35
Author(s):  
DHARMA RAJ MARASINI ◽  
JITENDRA PANDEY ◽  
LAXMAN PAUDEL SHARMA ◽  
LAXMI PAUDEL ◽  
ROSHNI GYAWALI ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Test Group ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Latency Time ◽  
Hot Plate ◽  
Ficus Religiosa ◽  
Standard Drug ◽  
Ethanolic Extracts

Objective: Because of adverse side effects, caused by NSAIDs, tolerance, and dependence induced by opiates, the use of these analgesic agents has not been successful in all cases. Therefore, alternative analgesic drugs from plant sources are the new target now days. The objective of this study was to evaluate the analgesic activity of ethanolic extracts of stem barks and leaves of Ficus religiosa. Methods: The analgesic activity of ethanolic extract of stem barks and leaves was evaluated in the Swiss albino mice model using acetic acid-induced writing response and Eddy’s hot plate method. Analgesic activity was demonstrated with the percentage inhibition of acetic acid induced writings and the percentage increased in latency time of paw licking. The potency of test extracts was compared with standard drug, Diclofenac. Results: Ethanolic extract of leaves and bark of F. religiosa showed potential analgesic activity from both methods. From Eddy’s hot plate model, it was observed that the percentage of increased latency time at 90 min by ethanolic extract of leaves and stem bark was found to be 70.81 % (8.54 min) and 70.78 % (8.53 min) respectively at a dose of 400 mg/kg. Both of these results are statistically significant (p<0.05) as compared to the test group. Furthermore, both of these extracts showed the dose-dependent and time-dependent increased in latency time and these results are compared to that of standard drug Diclofenac. Similarly, ethanolic extract of leaves and stem at 400 mg/kg significantly inhibited the number of writhings induced by acetic acid. The percentage inhibition of writhings by ethanolic extract of leaves at a dose of 400 mg/kg was 68.47 % which was similar to that of standard drug Diclofenac (68.47 %). However, ethanolic extract of bark showed relatively lower percentage inhibition (60.79 %) as compared to leaf extract and standard, but the result was significant as compared to that of the test group (p<0.05). Conclusion: Ethanolic extracts of F. religiosa stem bark and leaf possess both central and peripheral analgesic properties and these effects may be beneficial for the management of pain.

Evaluation of the Anti-Inflammatory and Analgesic Activities of the Total Saponins Extracted from Fermented Polygala japonica Houtt

2013 ◽  
Vol 641-642 ◽  
pp. 931-934
Author(s):  
Ming Xing Liu ◽  
Ting Zhu ◽  
She Ne Guo ◽  
Hong Da Zhu
Keyword(s):  
Experimental Study ◽  
Acetic Acid ◽  
Vascular Permeability ◽  
Analgesic Activity ◽  
Inflammatory Activity ◽  
Hot Plate ◽  
Plate Models ◽  
Anti Inflammatory ◽  
Analgesic Activities

This study was to investigate the anti-inflammatory and analgesic activities of the total saponins extracted from fermented Polygala japonica Houtt (FPH) compared with that of unfermented Polygala japonica Houtt (UFPH). The total saponins extracted from FPH and UFPH were evaluated for anti-inflammatory activity in xylene-induced ear swelling and acetic acid-induced vascular permeability models in mice, analgesic activity in acetic acid-induced writhing and hot plate models in mice. The total saponins extracted from FPH had the significant anti-inflammatory (p<0.001) and analgesic (p<0.01) activities with the doses of 6 g/kg b.w. in mice. The results of this experimental study thus strongly support the potential significant use of the total saponins extracted from FPH for pain and inflammatory.

scholarly journals Evaluation of analgesic activity of turmeric (Curcuma longa Linn.) in Wister rats

2017 ◽  
Vol 6 (3) ◽  
pp. 568 ◽  
Author(s):  
Sangita Jogdand ◽  
Jagruti Bhattacharjee
Keyword(s):  
Analgesic Activity ◽  
Normal Saline ◽  
Curcuma Longa ◽  
Ethanolic Extract ◽  
Albino Rats ◽  
Time Intervals ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Analgesic Activities

Background: NSAIDs like Aspirin etc. are randomly used for mild to severe types of pain but long-term and injudicious use of NSAIDs lead to a number of side effects. The present study is designed for exploring the analgesic potential of Curcuma longa Linn (Turmeric) in albino rats, which may widen the therapeutic horizon for the said agent.Methods: Ethanolic extract of Curcuma longa in the doses 100, 200 and 400mg/kg is given orally to 6 Wister rats against a control of normal saline and a standard using Aspirin (300mg/kg) and the animals were subjected to Eddy’s hot plate test at different time intervals i.e., 30, 60, 90 and 120 minutes after administration of the drugs and the parameters were noted.Results: The analgesic activity of Curcuma longa showed significant (p<0.05) increase in mean basal reaction time in Hot plate method when compared to the control (Normal saline). As the dose of the ethanolic extract of Curcuma longa was gradually increased from 100, 200 and 400mg/kg respectively, the analgesic activity significantly increased (<0.05). Effect of curcuma longa at a dose of 400mg/kg is found to be comparable with Aspirin.Conclusions: The results of this study suggest that turmeric (Curcuma longa) has significant analgesic activities in rats. 

scholarly journals AKTIVITAS ANALGESIK EKSTRAK ETANOL BULU BABI (ECHINOMETRA MATHAEI) PADA MENCIT PUTIH JANTAN

2020 ◽  
Vol 1 (02) ◽  
pp. 7
Author(s):  
Lestiono Lestiono ◽  
Angelica Kresnamurti
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Negative Control ◽  
Test Method ◽  
Flavonoid Compound ◽  
Writhing Test ◽  
Peripheral Pain ◽  
Echinometra Mathaei

Sea urchin (Echinometra mathaei) is a potential marine resourches that had not been explored widely. It has flavonoid, triterpenoid, alkaloid, saponin compound with high antioxidant activity. Antioxidant activity and flavonoid compound were potentially correlated with analgesic activity. This study aimed  to examine e the potential of Echinometra mathaei as an analgesic in white male mice that were induced by acetic acid to produce peripheral pain in vivo (streatching in mice) with writhing test method. The amount of stretching was observed for 1 hour after administration of ethanol extract of Echinometra mathaei orally. Balb/c white mice which devided into 6 groups randomlly, as negative control group, a group that induced acetic acid 1%,  Asetosal group with dose of 65 mg/kgBW, and Echinometra Mathaei groups with the dose of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. The results of this study showed that all dosage of Echinometra mathaei decrease the peripheral compared to negative control (P,0.05). The percentage inhibition of pain  at 200 mg/kgBW (72.59%) was the highest among other doses was significant different from asetosal group (52.53%). This research concluded that Echinometra mathaei has the analgesic activity to inhibit peripheral pain .    Keywords: Echinometra mathaei, ethanol extract, analgesic, mice, writhing test

scholarly journals In vivo Analgesic activity of methanolic extract of Helianthus annuus seeds

2016 ◽  
Vol 5 (4) ◽  
pp. 38-40 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Ahmed Tanjimul Islam ◽  
Mir Monir Hossain ◽  
Kishor Mazumder
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Helianthus Annuus ◽  
Analgesic Activity ◽  
Latency Period ◽  
Pharmaceutical Journal ◽  
Mice Model ◽  
Hot Plate ◽  
Writhing Test

The sunflower seed is the seed of the sunflower (Helianthus annuus). The methanol extract of seeds of Helianthus annuus were screened for analgesic activity in mice model to systematically explore the medicinal values of the plant. Acetic acid induced writhing and hot plate methods were used to confirm the central and peripheral analgesic action. In case of acetic acid-induced writhing test the extract showed significant (P <0.05) analgesic potential at doses 100 and 200 mg/kg body weight (50.35 and 57.85% inhibition, respectively). In the hot plate method, increase (p < 0.05) of latency period was also observed in comparison to standard aspirin. At 60 minutes, the latency period of two different doses (100 and 200 mg/kg body weight) was found at 13 ± 0.91 and 16.5 ± 1.55 second. The results obtained support the use of Helianthus annuus seeds in painful conditions acting both centrally and peripherally.Islam et al., International Current Pharmaceutical Journal, March 2016, 5(4): 38-40http://www.icpjonline.com/documents/Vol5Issue4/02.pdf

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