scholarly journals AKTIVITAS ANALGESIK EKSTRAK ETANOL BULU BABI (ECHINOMETRA MATHAEI) PADA MENCIT PUTIH JANTAN

2020 ◽  
Vol 1 (02) ◽  
pp. 7
Author(s):  
Lestiono Lestiono ◽  
Angelica Kresnamurti
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Negative Control ◽  
Test Method ◽  
Flavonoid Compound ◽  
Writhing Test ◽  
Peripheral Pain ◽  
Echinometra Mathaei

Sea urchin (Echinometra mathaei) is a potential marine resourches that had not been explored widely. It has flavonoid, triterpenoid, alkaloid, saponin compound with high antioxidant activity. Antioxidant activity and flavonoid compound were potentially correlated with analgesic activity. This study aimed  to examine e the potential of Echinometra mathaei as an analgesic in white male mice that were induced by acetic acid to produce peripheral pain in vivo (streatching in mice) with writhing test method. The amount of stretching was observed for 1 hour after administration of ethanol extract of Echinometra mathaei orally. Balb/c white mice which devided into 6 groups randomlly, as negative control group, a group that induced acetic acid 1%,  Asetosal group with dose of 65 mg/kgBW, and Echinometra Mathaei groups with the dose of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. The results of this study showed that all dosage of Echinometra mathaei decrease the peripheral compared to negative control (P,0.05). The percentage inhibition of pain  at 200 mg/kgBW (72.59%) was the highest among other doses was significant different from asetosal group (52.53%). This research concluded that Echinometra mathaei has the analgesic activity to inhibit peripheral pain .    Keywords: Echinometra mathaei, ethanol extract, analgesic, mice, writhing test

scholarly journals Perbandingan Aktivitas Analgetik Infusa dan Ekstrak Etanol Umbi Akar Tawas Ut (Ampelocissus rubiginosa Lauterb.)

2019 ◽  
Vol 6 (2) ◽  
pp. 40
Author(s):  
Khoerul Anwar ◽  
Muhammad Riswandi ◽  
Nurlely Nurlely
Keyword(s):  
Analgesic Activity ◽  
Acetic Acid Solution ◽  
Ethanol Extract ◽  
Negative Control ◽  
Tuberous Root ◽  
Control Group ◽  
Coarse Powder ◽  
Writhing Test ◽  
Group I ◽  
Root Tuber

ABSTRAK Umbi akar tawas ut (Ampelocissus rubiginosa Lauterb.) secara empiris digunakan untuk mengurangi nyeri. Masyarakat menggunakannya dengan cara meminum air seduhannya. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas analgetik dari infusa dan ekstrak etanol umbi akar A. Rubiginosa. Uji analgetik dilakukan menggunakan metode geliat (Writhing test) dengan pembanding parasetamol. Tiga puluh ekor mencit dibagi 6 kelompok masing-masing 5 ekor per kelompok. Kelompok I kontrol positif (parasetamol 65,25 mg/kgBB), kelompok II kontrol negatif (Na-CMC), kelompok III infusa A. rubiginosa 25 ml/kgBB, dan kelompok IV ekstrak etanol A. rubuginosa 500 mg/kgBB. Sesudah diberi perlakuan secara per oral sesuai kelompoknya, 30 menit kemudian diinduksi dengan asam asetat secara intraperitoneal. Jumlah geliat dihitung setiap 5 menit setelah pemberian larutan asam asetat 1% dengan selama 1 jam. Hasil penelitian menunjukkan persen proteksi pemberian parasetamol 65,25 mg/kgBB, infusa A. rubiginosa 65,25 mg/kgBB dan ekstrak etanol A. rubiginosa 500 mg/kgBB secara berurutan adalah 76,04; 87,41 dan 63,77%. Dari penelitian dapat disimpulkan bahwa infusa umbi akar A. rubiginosa memiliki aktivitas analgetik yang kuat. Kata kunci: Ampelocissus rubiginosa Lauterb., analgetik, infusa, ekstrak etanol  ABSTRACT Tuberous root of tawas ut (Ampelocissus rubiginosa Lauterb.) empirically used to reduce pain. People use it by drinking boiled water of A. rubiginosa coarse powder. This study aims to determine the comparison of analgesic activity of infusion and ethanol extract of A. rubiginosa tuberous root. Analgesic test was performed using a stretching method (Writhing test) with paracetamol as comparison. Thirty mice were divided into 6 groups of 5 individuals per group. Group I was positive control (paracetamol 65.25 mg / kgBW), negative control group II (Na-CMC), group III A. rubiginosa infusion 25 ml / kgBW, and group IV ethanol extract A. rubiginosa 500 mg / kgBW. After being treated orally according to the group, 30 minutes later induced with acetate acid intraperitoneally. The amount of stretching was calculated every 5 minutes after giving 1% acetic acid solution for 1 hour. The results showed percent protection of paracetamol 65.25 mg / kgBB, A. rubiginosa infusion 65.25 mg / kgBB and ethanol extract A. rubiginosa 500 mg / kgBB was 76.04; 87.41 and 63.77% respectively. From the research it can be concluded that A. rubiginosa root tuber infusion has a strong analgesic activity. Keyword: Ampelocissus rubiginosa Lauterb., analgetic, infusa, ethanol extract

scholarly journals Analgesic, Anti-Inflammatory, and GC-MS Studies onCastanospermum australeA. Cunn. & C. FraserexHook.

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Thankarajan Sajeesh ◽  
Thangaraj Parimelazhagan
Keyword(s):  
Acetic Acid ◽  
Analgesic Activity ◽  
Ethanol Extract ◽  
Hot Plate ◽  
Plate Method ◽  
Writhing Test ◽  
Paw Oedema ◽  
Soxhlet Apparatus ◽  
Anti Inflammatory ◽  
Ethanol Extracts

The present study was aimed to evaluate the analgesic and anti-inflammatory properties ofCastanospermum australeand to profile phytochemicals by GC-MS. The ethanolic extracts were prepared by successive solvent extraction using Soxhlet apparatus. The analgesic activity was analyzed by hot plate method and acetic acid-induced writhing test whereas anti-inflammatory study was done by carrageenan induced paw oedema model. The acute toxicity study revealed that ethanol extracts of leaf and bark ofC. australewere safe even at a higher dose of 2000 mg/kg whereas ethanol extract of seed was toxic at the same dose. In both hot plate method (5.85 s) and acetic acid-induced writhing test (57%), the leaf ethanol extract exhibited significant analgesic activity (P<0.001) at a dose of 400 mg/kg. The anti-inflammatory activity of leaf extract was exhibited by the reduction in paw linear diameter by 64.76% at 400 mg/kg in carrageenan induced paw oedema. The GC-MS analysis of the ethanol extract of leaf revealed sixteen major compounds of which 1,7-dimethyl-4,10-dioxa-1,7-diazacyclododecane, (+)-N-methylephedrine, and permethylspermine were found to be pharmaceutically and the most important. These findings justify thatC. australecan be a valuable natural analgesic and anti-inflammatory source which seemed to provide potential phytotherapeutics against various ailments.

Analgesic Effectivty Of Nanoparticles Chitosan-Ethanol Leaf Extract Papaya (Carica Papaya L.) In White Male Mice (Mus Mucculus)

2017 ◽  
Vol 15 (2) ◽  
pp. 228
Author(s):  
Juliferd Gredi ◽  
Wintari Taurina ◽  
Mohamad Andrie
Keyword(s):  
Leaf Extract ◽  
Carica Papaya ◽  
White Male ◽  
Ethanol Extract ◽  
Test Method ◽  
Ionic Gelation ◽  
Male Mice ◽  
Writhing Test ◽  
Effi Ciency ◽  
Optimal Formula

Papaya leaves has been shown to have efficacy as an analgesic. Analgesic effect caused by the flavonoids in leaves of papaya. To enhance the analgesic effi cacy papaya into dosage formulatio of nanoparticles. The purpose of this research was to create and characterized nanoparticles from ethanol extract of papaya leaves then tested for effectiveness as an analgesic. This study used polymer chitosan and NaTPP to created nanoparticles through ionic gelation method and analgesic activity tested using writhing test method and statistical tested. Optimal formula of nanoparticles were produced at concentration of 0.2% chitosan, Na-TPP extract 0.1% and 4% with a ratio of 6: 1: 1. The nanoparticles was formed a size of 255.4 nm with polidispers index of 0.7, the zeta potential of + 11,1mV, the adsorption effi ciency by 87% and rounded morphology (spherical). Analgesic effectiveness tested by comparison between the groups ethanol extract of papaya and the groups nanoparticles of chitosan-ethanol extract papaya statistically using the T-test Independent. The results of this research indicate that there are statistically signifi cant differences (p <0.05). This results shows that the preparation nanoparticles of chitosan-ethanol extract papaya is more effective as an analgesic than the ethanol extract of papaya leaves.

scholarly journals UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

scholarly journals Anti-nociceptive Potential of Ethanol Extract of Terminalia macroptera Guill&Perr (Combretaceae) Stem Bark in Mice

2021 ◽  
Vol 16 (2) ◽  
pp. 13-21
Author(s):  
S.A. Atunwa ◽  
M.O. Amali ◽  
S.O. Lawal ◽  
S.O. Usman ◽  
A.I. Olapade
Keyword(s):  
Acetic Acid ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Stem Bark ◽  
Negative Control ◽  
Flowering Plant ◽  
Control Group ◽  
Group I ◽  
Tail Immersion ◽  
Dose Dependent

Background: Terminalia macroptera Guill. &Perr. (Combretaceae) is a flowering plant with several ethno-medicinal claims. However, the dearth of information on its analgesic property has necessitated this study.Objectives: to evaluate the anti-nociceptive potential of ethanol extract of Terminalia macroptera stem bark (TMSB) in mice.Materials and Methods: Male and female mice of weight range 22 – 25g were randomly allotted into seven groups (n= 5) and treated as follows: Group I received 0.5 mL distilled water orally (negative control), Groups II-V were orally administered ethanol extract of T. macroptera stem bark (TMSB) at 50, 100, 200, and 400 mg/kg respectively while groups VI-VII received piroxicam 10 mg/kg and pentazocine 2 mg/kg intraperitoneally respectively as standards. The same treatment pattern was adopted for both pain models: tail immersion and acetic acid-induced writhing assays. Data were expressed as mean ± standard error of mean (SEM) using two-way analysis of variance (ANOVA) followed by Tukey’s and Bonferroni's multiple comparisons tests with p < 0.05 taken as significance.Results: The ethanolic extract of Terminalia macroptera stem bark showed significant dose-dependent anti-nociceptive activity at 100 and 400 mg/kg (2.95±0.41 and 2.9±0.31 respectively) 60 min post-treatment compared to the negative control group in the tail immersion test. Significant inhibition of nociception (0.20±0.20) was obtained at 400 mg/kg compared to the negative control group in the acetic acid-induced writhing test.Conclusions: The ethanol extract of Terminalia macroptera stem bark exhibited dose-dependent anti-nociceptive potential in both tail immersion and acetic acid-induced writhing assays in mice.

The Role of Andrographolide in Andrographis paniculata as a Potential Analgesic for Herbal Medicine based Drug Development

2021 ◽  
pp. 6269-6274
Author(s):  
Lidya Tumewu ◽  
Irfan Rayi Pamungkas ◽  
Hilkatul Ilmi ◽  
Achmad Fuad Hafid ◽  
Indah Setyawati Tantular ◽  
...  
Keyword(s):  
Body Weight ◽  
Acetic Acid ◽  
Herbal Medicine ◽  
Drug Development ◽  
Analgesic Activity ◽  
Active Ingredient ◽  
Active Substance ◽  
Ethanol Extract ◽  
Positive Control ◽  
Andrographis Paniculata

Background: Andrographis paniculata is a herbaceous plant in the Acanthaceae family, that is widely used as a traditional medicine in Asian countries and known to exhibit a wide range of pharmacological effects. Recent studies have provided an overview of the great potential of A. paniculata as an analgesic. The ethanol extract and ethyl acetate (EA) fraction of A.paniculata were shown to contain diterpene lactone compounds, which may be useful as a potential active ingredient in analgesic drugs. The development of a herbal medicine based drug requires an effective and high quality active ingredient. Therefore, this research was aimed to compare the analgesic activity of ethanol extract and EA fraction based on their andrographolide content and further to determine the more viable active substance for analgesic herbal medicine based drug development. Method: The andrographolide content in the ethanol extract and EA fraction was determined by High Pressure Liquid Chromatography (HPLC). Measurement of analgesic activity was performed by writhing test. The experimental animals were randomly divided into eight groups consisting of 5 mice in each. Group 1 (negative control) received 1% Tween-80 in normal saline. Group 2 (positive control) received a standard analgesic drug (diclofenac sodium) at a dose of 40 mg/kg body weight. Group 3, 4, and 5 received ethanol extract while Group 6, 7, and 8 received EA fraction, each at a dose of 12.5, 25, and 50 mg andrographolide/kg body weight, respectively. Each mouse was injected intraperitoneally with 1% acetic acid at a dose of 10 ml/kg body weight 30 minutes after oral administration of the treatments. The number of writhes were counted 5 min after acetic acid injection over a period of 45 min. Results: Andrographolide content in ethanol extract and EA fraction was 15.66±0.28 and 21.25±1.08 % w/w, respectively. Ethanol extract and EA fraction displayed analgesic activity of 67.68% and 70.91% respectively, at a dose of 50 mg andrographolide/kg body weight. The positive control at a dose of 40 mg/kg body weight showed an analgesic activity of 74.33%. Statistical analysis showed no significant differences between EA fraction at a dose of 50 mg andrographolide/kg body weight and ethanol extract at the same dose as well as the positive control (P> 0.05). The effective dose 50% (ED50) of the ethanol extract and EA fraction was determined to be 29.49 and 25.55 mg/kg body weight, respectively. Conclusion: It was possible to use andrographolide content as an indicator for the analgesic activity of A.paniculata. Ethanol extract and EA fraction of A. paniculata at the same dose of andrographolide showed similar analgesic activity. The amount of ethanol extract which needed to reach similar analgesic activity was higher than EA fraction. Therefore, EA fraction likely has greater potential as an analgesic active substance due to its higher content of andrographolide; however further study is needed to develop it as a dosage form.

scholarly journals In vivo and in vitro pharmacological activities of Tacca integrifolia rhizome and investigation of possible lead compounds against breast cancer through in silico approaches

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Shahriar Ahmed ◽  
Ahmed Rakib ◽  
Md. Ashiqul Islam ◽  
Bibi Humayra Khanam ◽  
Farhana Binta Faiz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Acetic Acid ◽  
Molecular Docking ◽  
Analgesic Activity ◽  
In Silico ◽  
Antineoplastic Activity ◽  
Paw Edema ◽  
Writhing Test ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Abstract Background The study was conducted to evaluate the analgesic, anti-inflammatory, antipyretic properties of ethanolic extract of Tacca integrifolia rhizome (EETI) in mice and cytotoxic effects in brine shrimp nauplii followed by a PASS prediction study for some isolated compounds of T. integrifolia. Additionally, this experiment included the in silico molecular docking and ADME/T property analyses of some phytochemicals. Methods Formalin- induced paw licking test and acetic acid-induced writhing test for analgesic activity, carrageenan-induced paw edema test for anti-inflammatory potential and Brewer’s yeast-induced pyrexia test for antipyretic activity were applied. Antinociceptive and antineoplastic activity for breast cancer were revealed with PASS program. Schrodinger suite 2015 was used to evaluate the binding interaction and ADME/T properties of selected phytoconstituents with estrogen receptor alpha. Results In formalin-induced paw licking test, EETI at the doses of 200 and 400 mg/kg BW showed highly significant inhibition of writhing in both neurogenic and inflammatory phases. While EETI also exhibited highly significant, compared to control, writhing inhibition for both the doses in acetic acid-induced writhing test. Moderate anti-inflammatory effect at a dose of 400 mg/kg BW was noticed in paw-edema test. It also showed 77.51% of maximum antipyretic effect which was significantly effective compared to standard drug paracetamol (150 mg/kg) in Brewer’s yeast-induced pyrexia test. The EETI showed potential cytotoxic activity with LC50 value of 114.46 μg/mL. The PASS prediction revealed the potential antinociceptive and antineoplastic activity of target compounds. The compounds betulinic acid, quercetin-3-α-arabinoside, catsanogenin were found to be effective in molecular docking study. Conclusion It is evident that the EETI possesses highly significant analgesic activity with remarkable anti-inflammatory and antipyretic activity. The phytoconstituents have potential antinociceptive and antineoplastic (breast cancer) activity. Graphical abstract

scholarly journals KULIT BUAH JERUK LIMAU (Citrus amblycarpa (Hassk.) Osche) SEBAGAI ANALGESIK

Jurnal Kimia ◽  
2020 ◽  
pp. 24
Author(s):  
R. A. I. K. Maharani ◽  
N. K. Cahyaningsih ◽  
M. D. Abimanyu ◽  
K. W. Astuti
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Treatment Options ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Fruit Peel ◽  
Hot Plate ◽  
Inflammatory Drugs ◽  
Negative Controls

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the treatment options for relieving pain. However, long-term use can trigger gastrointestinal bleeding. Therefore, alternative analgesics which have the same therapeutic effect with lower side effects are needed. Limau (Citrus amblycarpa) is an empirical drug for tingling and cramping. The aim of the study is to determine the analgesic activity of ethanol extract of C. amblycarpa fruit peel. The method used in testing analgesic activity is the Hot Plate method. The study was conducted by dividing 30 mice into 6 groups. The group given CMC-Na 1% was used as a negative control, the group given suspension of sodium diclofenac dose of 6.5 mg/kg of body weight was used as a positive control, and the group given suspension of ethanol extract of C. amblycarpa fruit peel with dose variations 100, 300 and 600 mg/kg of body weight. The test animals were placed on top of the Hot Plate with a temperature of 70°C at 30 minutes after giving suspension test and the response time of mice to heat was observed every 30 minutes for 3 hours with cut off time 15 second. Based on the test results, it can be concluded that the administration of ethanol extract of C. amblycarpa fruit peel with 100, 300 and 600 mg/kg of body weight gave analgesic activity on mice compared to the negative controls (CMC-Na 1%).   Keywords: C. amblycarpa, Fruit Peel, Analgesics, Hot Plate 

scholarly journals Analgesic Activity of Ethanol Extract of Temu Giring Rhizome (Curcuma heyneana Val & Zijp) in Mice

2018 ◽  
Vol 1 (2) ◽  
pp. 9-13
Author(s):  
Marianne ◽  
Khairunnisa ◽  
Wilda
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Significance Level ◽  
Treatment Groups ◽  
Infra Red ◽  
Thermal Induction

Temu giring (Curcuma heyneana Val & Zijp) is a traditional medicinal plant that is believed in community as an analgesic. The objective of this research was to determine the analgesic activity of the C. heyneana rhizome by using infra red (IR) thermal induction method in mice. Mice were divided into 7 groups. Group 1 served as negative control, group 2,3,4,5 served as treatment groups which is  given ethanolic extract of C. heyneana rhizome at  dose of 5, 25, 125, and 625 mg/kg respectively, group 6 and 7 served as  comparable groups, given antalgin 65 mg/kg and morphine sulphate 1.3 mg/kg respectively. The observation have been done, included to pain resistance of mice which exposed by infra red (IR) every 10 minutes for 80 minutes. The data were analyzed by ANOVA at the significance level of 95%. Ethanolic extract of C. heyneana at the doses of 25, 125, and 625 mg/kg had significant effect to reduce the pain compared to the negative control (p<0.05). Ethanolic extract of C. heyneana rhizome at dose of 125 mg/kg, had the same effect to antalgin 65 mg/kg  (p≥0.05), while the ethanolic extract of C. heyneana at the dose of 625 mg/kg had the same effect as morphine sulfate 1.3 mg/kg (p≥0.05). It can be concluded that ethanolic extract of C. heyneana rhizome has analgesic activity.   Keywords: temu giring, analgesic, Curcuma heyneana, rhizome

scholarly journals Aktivitas Antihiperglikemik dari Ekstrak Etanol dan Heksana Tumbuhan Suruhan (Peperomia pellucida [L.] Kunth) pada Tikus Wistar (Rattus norvegicus L.) yang Hiperglikemik

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 109
Author(s):  
Sariyana Togubu ◽  
Lidya I. Momuat ◽  
Jessy E. Paendong ◽  
Navila Salma
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Tolerance Test ◽  
Negative Control ◽  
Hexane Extract ◽  
Test Method

Telah dilakukan penelitian kadar glukosa darah tikus wistar yang hiperglikemia pada pemberian ekstrak etanol dan heksana tumbuhan suruhan (Peperomia pellucida [L]. Kunth). Penelitian ini menggunakan metode uji toleransi glukosa, terhadap 16 ekor tikus jantan wistar yang hiperglikemia akibat diinduksi sukrosa. Selanjutnya, tikus dibagi dalam 4 kelompok perlakuan yaitu kelompok kontrol negatif (K-) diberi CMC 0,5%, kelompok kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok ekstrak etanol tumbuhan suruhan (EETS) dosis 40 mg/kgBB, dan kelompok ekstrak heksana tumbuhan suruhan (EHTS) dosis 40 mg/kgBB. Setiap kelompok terdiri dari 4 ekor tikus. Kadar glukosa darah tikus diukur dengan alat Drglukometer pada menit ke-30, 60, dan 120 setelah diberikan perlakuan. Hasil penelitian menunjukkan bahwa pada menit ke-120, kadar glukosa darah tikus pada kelompok EETS dan EHTS masing-masing turun sebesar 54,57% dan 51,25%, dan tidak berbeda nyata dengan K(+). Kadar glukosa darah kelompok K(+) dan EETS pada menit ke-120 mencapai kadar yang sama dengan keadaan basal (normal). Penelitian ini menyimpulkan bahwa ekstrak etanol dan heksana tumbuhan suruhan dengan dosis 40 mg/KgBB memiliki efek hipoglikemik pada tikus jantan wistar yang hiperglikemia.A study on blood glucose level in hyperglycemic wistar rats treated with ethanol and hexane extracts of suruhan (Peperomia pellucida [L]. Kunth) had been done. This study used glucose tolerance test method applied on 16 male wistar rats which were hyperglycemic by sucrose induction. The rats were divided into four treatment groups, each of which contained four rats, which were negative control (K-) group treated with CMC 0.5%, positive control (K+) group treated with glibenclamide 0.45 mg/kg body weight, group treated with ethanol extract of P. pellucida (EETS) 40 mg/kg body weight, and group treated with hexane extract of P. pellucida (EHTS) 40 mg/kg body weight. Glucose level in rat blood was measured using Drglucometer at minute 30, 60, and 120 after treatment. The results showed that at minute 120 the blood glucose level of EETS and EHTS was decreased by 54.57% and 51.25%, respectively, which were not significantly different from K(+). At minute 120, blood glucose of K(+) and EETS reached the same level with that of basal (normal) state. It was concluded that ethanol and hexane extract of P. pellucida 40 mg/kg body weight had hypoglycemic effect on hyperglycemic wistar rats.

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