scholarly journals Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus) terhadap Kadar Glutathion Dan Interleukin 6 Serum Tikus Wistar Jantan yang Diberi Pakan Tinggi Kolesterol

2020 ◽  
Vol 11 (1) ◽  
pp. 77
Author(s):  
I Gusti Agung Dewi Sarihati ◽  
I Gusti Ayu Sri Dhyanaputri
Keyword(s):  
Interleukin 6 ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Male Rats ◽  
P Value ◽  
High Cholesterol ◽  
Endothelial Cell Function ◽  
Cosmos Caudatus ◽  
Male Wistar Rats

<p>Chronic dyslipidemia, especially in hypercholesterolemia, can directly disrupt endothelial cell function through increased formation of oxidized LDL which will stimulate an inflammatory response including interleukin 6 (IL-6) release. For this reason, it is necessary to have anti-inflammation that can reduce the effects of hypercholesterolemia on blood vessels. Polyphenols have anti-inflammatory effects and kenikir leaves are vegetables that are rich in polyphenols. This study aims to prove the effect of kenikir leaf (<em>Cosmos caudatus</em>) ethanol extract on IL-6 levels of serum of male Wistar rats fed high cholesterol. The method used is a true experimental study with a Post-test Only Control Group Design. Samples using 24 male rats were divided into 4 groups DS, DTK, Ss, and Cc. DTK, Ss and Cc groups were given high cholesterol feed for 20 weeks. In the 17th to 20th weeks the Ss group was given additional simvastatin and the Cc group was given an additional extract of <em>Cosmos caudatus</em> leaf. Whereas the DS group was only given standard feed for 20 weeks. After 20 weeks all rats in all four groups were examined serum GSH and IL-6 levels. The results showed the highest GSH serum levels average in Cc with p-value=0,016 but not different from Ss. IL-6 serum levels in DTK were higher than those of the other three groups and were significantly different with p-value=0,035. The difference occurs between DTK and DS, Ss and Cc, not different between DS, Ss and Cc. The ethanol extract of kenikir leaf influences the serum IL-6 levels of male Wistar rats fed high cholesterol.</p>

Safety Evaluation of Crude Ethanol Extract of Waltheria Indica Linn. Root In Male Wistar Rats

2021 ◽  
Vol 3 (1) ◽  
pp. 112-125
Author(s):  
A. Basiru
Keyword(s):  
Wistar Rats ◽  
Safety Evaluation ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Hepatic Necrosis ◽  
Control Group ◽  
Percentage Increase ◽  
Male Wistar Rats ◽  
Group A ◽  
Renal Tubular

Safety evaluation of Waltheria indica Linn. root was carried out in Wistar rats. Twenty adult male Wistar rats were randomly divided into four groups (n=5); A–D. Group A served as control group while groups B, C and D were administered with 200, 400 and 800 mg/Kg of crude ethanol extract of Waltheria indica Linn. root. After 28 days of administration, the rats were sacrificed and blood samples were collected and serum prepared. Haematological and biochemical parameters, as well as serum electrolyte were determined according to standard procedures. The 200 mg/Kg of Waltheria indica Linn. root caused the highest percentage increase in body weight. The kidney was also significantly increased at 400 mg/kg. The total WBC and lymphocytes counts were significantly increased at 400 mg/kg. The serum levels of ALT and chloride were significantly increased at 800 mg/kg. Waltheria indica Linn. root also caused hepatic necrosis and renal tubular degeneration in Wistar rats. Therefore, Waltheria indica Linn. root should be used with caution especially at higher dose despite its therapeutic potentials.

scholarly journals Centella asiatica ethanol extract increases hippocampal brain derived neurotrophic factor in male Wistar rats

2018 ◽  
Vol 37 (2) ◽  
pp. 143
Author(s):  
Astri Handayani ◽  
Sophie Yolanda ◽  
Ria Kodariah
Keyword(s):  
Synaptic Plasticity ◽  
Protein Expression ◽  
Adult Male ◽  
Wistar Rats ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Memory Formation ◽  
Male Rats ◽  
Hippocampal Region ◽  
Male Wistar Rats

Background<br />Synaptic plasticity, which primarily takes place in the hippocampus, is the molecular basis of long- term memory formation. Brain derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays a significant role in synaptic plasticity and memory formation. When BDNF is released, it binds to its receptor and activates various intracellular signal transduction pathways leading to synaptic plasticity. Several methods to improve memory function in humans have been studied, one of which is the use of herbal compounds, such as Centella asiatica (CeA), an herbaceous plant that has been used for improving memory. This study aims to examine the effects of CeA ethanol extract on BDNF protein expression in the CA1 hippocampal region in adult male rats.<br /><br />Methods<br />A randomized experimental design was performed involving 18 adult male Wistar rats. The rats were randomized into three groups: one control/distilled water group and two groups treated with doses of CeA ethanol extract of 300 mg/kgBW (CeA300) and 600 mg/kgBW (CeA600), respectively. CeA ethanol extract was administered orally for 28 consecutive days with weekly weight-adjusted dose. After 28 days, the rats were decapitated, and the hippocampus was isolated from the brain. BDNF protein expression was assessed using immunohistochemistry. Data was analyzed using Kruskal-Wallis test and continued with post-hoc analysis. <br /><br />Results<br />There was a significant increase in BDNF protein expression in the CeA600 group compared to the control group (p&lt;0.001). <br /><br />Conclusion<br />Administration of CeA ethanol extract increased BDNF protein expression in the CA1 hippocampal region of adult male rats.

scholarly journals Evaluating the Effects of Noise Pollution on the Levels of Blood Cortisol, Testosterone, and Thyroid in Male Wistar Rats

Health Scope ◽  
2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Seyed-Hosein Abtahi-Eivary ◽  
Ali Tajpoor ◽  
Ali Firoozi ◽  
Shahrzad Mehrzad ◽  
Mohammad Hosein Beheshti
Keyword(s):  
Wistar Rats ◽  
Statistical Tests ◽  
Noise Pollution ◽  
Well Being ◽  
Serum Levels ◽  
Sound Level ◽  
Male Rats ◽  
Control Group ◽  
Male Wistar Rats ◽  
Commercial Kits

Background: Noise pollution is a global problem causing changes in the secretion of various hormones and consequently affecting social well-being and quality of life in cities. Objectives: This study aimed to investigate the effect of noise pollution on the levels of testosterone, thyroid, and cortisol hormones in male rats. Methods: In this experimental study, a total of 70 male Wistar rats (200 - 250 g) were randomly assigned into one control and six experimental groups, with 10 rats in each group. Experimental groups were exposed to noise with different intensity (dB) and time (min) as follows: (I) 60 dB, 30 min; (II) 60 dB, 60 min; (III) 85 dB, 30 min; (IV) 85 dB, 60 min; (V) 110 dB, 30 min; (VI) 110 dB, 60 min; (VII) controls. Animals in the experimental groups were exposed to noise in an acoustic chamber designed for this purpose for 50 days. The Noise.exe software was used to generate noise, and the sound level meter (model TES 1358) was used to determine the accuracy of the intensity and frequency of sound. To determine plasma levels of the hormones, appropriate research and commercial kits were used, which were based on the ELISA method. To determine the concentration of hormones other than TSH, human assay kits were used. All statistical tests were performed in SPSS software version 21. Results: Serum levels of cortisol in the 110-dB (30 and 60 min), 65-dB (60 min), and 85-dB (60 min) groups were significantly higher than the control group (P ≤ 0.05). Also, cortisol levels in the 65-dB and 85-dB (30 minutes) groups were higher than the control group; however, the increase was not significant (P > 0.05). The levels of T4, T3, and TSH in the 60-dB and 85-dB groups were significantly lower than in the control group (P ≤ 0.05). The serum levels of T4, T3, and TSH hormones in the 110-dB group were insignificantly lower than the control group (P > 0.05). The serum level of testosterone in the 110-dB group was significantly lower than the control group (P ≤ 0.05). The mean serum levels of testosterone in the 65-dB and 85-dB groups were insignificantly lower than the control group (P > 0.05). Conclusions: Based on this study, exposure to noise pollution increased cortisol secretion and decreased T4, T3, TSH, and testosterone levels in rats. As this hormonal imbalance may create direct and indirect effects, studies and strategies are recommended to control the imbalance of hormones in the polluted environments.

scholarly journals Pengaruh ekstrak melon terhadap kadar HbCO pada tikus Wistar jantan yang dipapar asap rokok

2017 ◽  
Vol 13 (3) ◽  
pp. 105
Author(s):  
Yuyun Erlina Susanti ◽  
Bambang Wirjatmadi ◽  
Merryana Adriani
Keyword(s):  
Cigarette Smoke ◽  
Wistar Rats ◽  
Smoking Behavior ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Adult Males ◽  
Control Group Design ◽  
Post Test ◽  
Male Wistar Rats

Background: Smoking is something that is usual for most Indonesian society, especially adult males. According to Riskesdas, smoking behavior of the population trends to increase. A previous study suggested that exposure to cigarette smoke produce CO bond to hemoglobin. Melon (Cucumis melo) contains antioxidants that prevent tissue damage.Objective: Analyze differences in levels HbCO Wistar male rats in treated and untreated extracts of melon.Method: The research was a laboratory experiment with the post test control group design and complete random design. The subjects were 25 male Wistar rats aged 3 months. Subjects were divided into 5 groups and each group received treatment for 28 days. Data of level HbCO was collected and analyzed by Anova One Way test at 95% confidence level.Results: The results showed a difference between the average levels of HbCO male Wistar rats (p value=0,000) treated and untreated extracts of melon.Conclusion: Melon extracts effect on decreasing the levels of HbCO due to exposure to cigarette smoke. There were differences in levels of male Wistar rats HbCO on treated and untreated extracts of melon and be given at a dose of 250 IU/day.

scholarly journals Bitter Leaves Ethanol Extract (Vernonia amygdalina) Decreases Total Cholesterol Serum Level of Hypercholesterolemic Male Wistar Rats

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Ivana C Hasan ◽  
Hendra Subroto ◽  
Grace Puspasari
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Plasma Lipid ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Control Group ◽  
Standard Diet ◽  
Group Vi ◽  
Significant Difference ◽  
Male Wistar Rats

Hypercholesterolemia is an important risk factors of atherosclerosis. Bitter leaf is believed to have potency in decreasing plasma lipid. The aim of this study is to study the effect of bitter leaf ethanol extract in decreasing total cholesterol serum levels on hypercholesterolemic male Wistar rats. This is a true experimental laboratory study with complete randomized design. Antidyslipidemia effect was tested with HFF induction and Propiltiourasil 0,01% towards 30 rats which were divided into 6 groups: groups I as normal control (standard diet), groups II, III, and IV were given bitter leaf ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW dose, group V as positive control group with Simvastatin, and group VI as negative with aquades. HFF  were given for 14 days then the study was continued for 14 days. The percentage data of the declining total cholesterol levels before and after treatments is analyzed with ANAVA, continued with LSD test. The result between group II, III, and IV with group VI shows a significant difference p<0,01. We concluded bitter leaf ethanol extract can decrease the level of total cholesterol serum of hypercholesterolmic Wistar rats.

scholarly journals Synthesis of Silver Nano-particles by Electrochemical Method and the Effects on the Serum Levels of Thyroid Hormones (T3, T4) in Adult Male Rats

2019 ◽  
Vol 13 (4) ◽  
pp. 21-25
Author(s):  
Zohreh Parang ◽  
◽  
Davood Moghadamnia ◽  
Keyword(s):  
Adult Male ◽  
Electrochemical Method ◽  
Wistar Rats ◽  
Serum Levels ◽  
Nano Particles ◽  
Male Rats ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Mean

Background: Silver nano-particles have anti-fungal properties. In the present study, silver nano-particles were synthesized by electrochemical method and its effects on the serum levels ofT3 and T4 hormones were investigated in adult male rats. Methods: In this experimental study, 28 adult male Wistar rats weighing approximately 180-220g were divided into 4 groups of 7 rats. The control group (no treatment). The experimental groups 1and 2 received silver intraperitoneal nano-particle doses of 25 and 100 mg/kg, respectively, for 14 days. The nano-aprticles had been synthesized at 75 seconds interval. The experimental group 3 received silver nano-particles that were synthesized at 300 seconds interval with an intraperitoneal dose of 25 mg/kg for 14 days. At the end of this period, blood samples were obtained from the rats’ hearts, and the serum levels of T3 and T4 hormones were measured. The results were statistically analyzed using ANOVA and Duncan tests. Results: At the completion of the study, there was no significant difference in the mean body weights in all experimental groups compared to control group. The results showed that the mean serum levels of T3 hormone in the experimental group1 increased significantly relative to the control group. However, there was no significant difference in the mean serum levels of T4 hormone in all experimental groups compared to that in the control group (P < 0.05). Conclusions: Silver nano-particles increased the serum T3 hormone level in male Wistar rats.

Anti-diabetogenic and in vivo antioxidant activity of ethanol extract of Dryopteris dilatata in alloxan-induced male Wistar rats

Biomarkers ◽  
2021 ◽  
pp. 1-15
Author(s):  
Akpotu E. Ajirioghene ◽  
Samuel I. Ghasi ◽  
Lawrence O. Ewhre ◽  
Olusegun G. Adebayo ◽  
Jerome N. Asiwe
Keyword(s):  
Antioxidant Activity ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Male Wistar Rats

scholarly journals Effect of different types of stress on adrenal gland parameters and adrenal hormones in the blood serum of male Wistar rats

2009 ◽  
Vol 61 (2) ◽  
pp. 187-194 ◽  
Author(s):  
M. Adzic ◽  
Ana Djordjevic ◽  
Jelena Djordjevic ◽  
Ana Niciforovic ◽  
Marija Radojcic
Keyword(s):  
Blood Serum ◽  
Wistar Rats ◽  
Adrenal Glands ◽  
Acute Stress ◽  
Serum Levels ◽  
Combined Stress ◽  
Adrenal Hormones ◽  
General Adaptation ◽  
Different Types ◽  
Male Wistar Rats

In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamine, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.

scholarly journals The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

2017 ◽  
Vol 15 (2) ◽  
pp. 196
Author(s):  
Much Ilham Novalisa Aji Wibowo ◽  
Nur Aeni ◽  
Zidna Mazayatul Huda ◽  
Nunuk Aries Nurulita
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Male Wistar Rats ◽  
Hepatoprotective Agent ◽  
Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

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