scholarly journals Seroprevalence of Human, Sheep and Goat brucellosis in Raparin District

2019 ◽  
Vol 7 (1) ◽  
pp. 58-79
Author(s):  
Rzgar Qadir Pirot ◽  
Adel Kamal Khdir
Keyword(s):  
Animal Species ◽  
Bacterial Disease ◽  
Age Group ◽  
Serological Tests ◽  
Middle East Countries ◽  
Total Prevalence ◽  
Gender And Age ◽  
Human Sera ◽  
The Rose ◽  
Positive Results

   Brucellosis is a worldwide disease caused by an infectious bacterial disease called Brucella, it has some species in genera. Prevalence of the brucellosis in many area of the world particularly in some Mediterranean and Middle East countries. A total 494 sera samples were collected from 36 herds in Raparin district including 113 human sera (herder), 199 sheep and 182 goat sera sample.Using three serological such as Rosbengal test (RBT), 2-Mercaptoethanol test (2ME) and Enzyme linked immune sorbent Assay (ELISA) detect the prevalence of brucellosis in the district according to animal species, gender, vet office area and age group with a comparison between serological tests which was used in this study during august 2017 till jun 2018 . All samples collected from adults of both sexes from different seven geographical areas in Raparin district. The total prevalence of brucellosis was 26.3% this value varied according to ifferent areas. The highest in Sangasar and Bngrd was 37.3%, and 35.3% while the lowest in Rnia and Zharawa was 13.2%, and 16.9% respectively, using the Rose-bengal test as a screening test to identify the prevalence of the disease. The total prevalence was varied according to the animal species, gender and age group. The study revealed that the prevalence of the disease varied according to the used type of the serological tests. RBT recorded the highest 26.3% then the 2-Mercapto-ethanol test recorded (14.4%) and ELISA recorded (12.4%), When comparing the positive results of ELISA and other serological tests the results reveals significant (P<0.05) difference between ELISA and other serological tests.

scholarly journals Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
James M. Akoko ◽  
Roger Pelle ◽  
AbdulHamid S. Lukambagire ◽  
Eunice M. Machuka ◽  
Daniel Nthiwa ◽  
...  
Keyword(s):  
Host Species ◽  
Animal Species ◽  
Control Strategies ◽  
Serum Samples ◽  
Cross Transmission ◽  
History Of ◽  
Human Ecosystems ◽  
Human Participants ◽  
The Rose ◽  
Positive Results

AbstractBrucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations.

Diabetes related disease and disorder in a Chinese Q&A website: Comparison on frequency and position (Preprint)

2020 ◽  
Author(s):  
Wei Feng ◽  
Ruocheng Huang ◽  
Shan Lu ◽  
Tao Shan ◽  
Hong Wang ◽  
...  
Keyword(s):  
Kidney Failure ◽  
Medical Information ◽  
Healthcare Management ◽  
Medical Community ◽  
Circulation System ◽  
Age Group ◽  
Disease Category ◽  
Old Patients ◽  
Gender And Age ◽  
Question And Answer

BACKGROUND With the development of the Internet, online medical community can help patient access to medical information and relevant decisions more conveniently, and meet the needs of patients for their own healthcare management. Mining these Q&A (Question and Answer) data, we can help doctors give more targeted feedback which improve the efficiency of question-and-answer, and patient satisfaction. OBJECTIVE This study aimed to (1) analysis frequency and position of diabetes related diseases or symptoms in Q&A website and (2) find out the differences of disease terms in gender and age using in the questions. METHODS We collected 5766 Q&A diabetes related data on the website of Chunyuyisheng from June 2012 to April 2020. In 38176 combined sentences, a vocabulary contains 3 categories of 3851 word and 2094 ICD (International Classification of Diseases) matching terms were obtained by calculating the similarity using word vectors. Proportion of the frequency of words and Mann-Whitney U test on word position were used to quantify the difference in patient’s gender and age group. RESULTS The vocabulary of the disease category accounts for 70%. We analyzed the word frequency and position in questions for different gender and age group. For gender, women participate in question answering more, accounting for 53% of total questions. They pay more attention to pregnancy, sleep and thyroid gland related vocabulary compared to men. Men focus more on circulation system, kidney failure related vocabulary. For different age group, pregnancy, glucose regulation, digestive and respiratory system related vocabulary have a higher proportion for patients under 40 years old. Patients over 40 years old pay more attention on kidney failure, cerebral ischaemia, infectious and circulation system. CONCLUSIONS This study provides a new insight into frequency and position of diabetes related diseases or symptoms in online medical services. It can show patients’ different attention by comparing disease or symptom categories for gender and age with ICD disease codes. The frequency and position of disease category words in patients’ conversation can be used for further risk evaluation for chronic diseases research.

scholarly journals 229 Sleep-wake behavior, meal timings and digital media duration of Indians during COVID-19 Lockdown

SLEEP ◽  
2021 ◽  
Vol 44 (Supplement_2) ◽  
pp. A91-A92
Author(s):  
Babita Pande ◽  
Meenakshi Sinha ◽  
Ramanjan Sinha
Keyword(s):  
Digital Media ◽  
Sleep Duration ◽  
Sleep Onset ◽  
Therapeutic Management ◽  
Daily Routine ◽  
Age Group ◽  
Younger Age ◽  
Gender And Age ◽  
Delayed Sleep ◽  
The Impact

Abstract Introduction Lockdown and stay home order has been imposed on people in many countries including India to prevent the community transmission of COVID-19 pandemic. However this social restriction led to disturbed daily routine and lifestyle behaviour that is needed to be attended for proper therapeutic management of overall health during such crisis. The impact of lockdown on the most apparent behavioral changes viz. sleep-wake behaviour, major meal timings, and digital screen duration of Indians were investigated. In addition the effects of gender and age were explored. Methods After seeking permission from Ethical Institution, an online questionnaire based survey was circulated within India in the first week of May, 2020 for which total 1511 male and female (age ≥18 years) subjects participated. The sleep-wake behavior observed were sleep-wake timings, sleep duration, mid sleep time (MST) as function of lockdown, and social (lockdown) jetlag (SJL = MST before lockdown-MST during lockdown). Results The sleep onset-wakeup and meal times were significantly delayed during lockdown, which was more pronounced in younger age group. The sleep duration increased, specifically in young individuals during lockdown. Females showed more delayed sleep onset-waking times and first meal timing with longer sleep duration during lockdown. Increased digital media duration was observed in all age groups, primarily in males. The younger age group and specifically female reported higher SJL and delayed MST. A positive association was obtained between sleep duration & first meal time, and SJL & major meal timings/screen duration, and a significant negative relationship of sleep duration and SJL with age. Conclusion The study shows delayed sleep-wake schedule, meal timings and increased digital media duration among Indians during COVID-19 lockdown compared to before lockdown. Also, gender and age emerged as important mediating factors for this alteration. The pandemic has given opportunity to sleep more and compensate for the sleep. In spite of that, the higher social jetlag in young age group and female showed the compromised sleep and maladaption with societal timing. These findings have applied implications in sleep health during longer social isolation conditions and for proper therapeutic management. Support (if any) No

scholarly journals Antibody responses in patients with farmer's lung disease to antigens fromThermoactinomyces vulgaris

1975 ◽  
Vol 74 (1) ◽  
pp. 35-41 ◽  
Author(s):  
M. R. Hollingdale
Keyword(s):  
Lung Disease ◽  
Sodium Periodate ◽  
Latex Agglutination ◽  
Antibody Responses ◽  
Muramic Acid ◽  
Farmer’S Lung ◽  
Farmer's Lung ◽  
Human Sera ◽  
Thermoactinomyces Vulgaris ◽  
Positive Results

SUMMARYA serological analysis of mycelial antigens ofThermoactinomyces vulgarisin immunodiffusion with human sera revealed five individual antigens. Three antigens were proteins, sensitive to pronase and soluble in phenol. Two were cationic polysaccharides, sensitive to sodium periodate, and containing glucosamine and muramic acid.Latex coated with mycelial antigens was compared with precipitin tests in detecting antibodies toT. vulgaris;the number of positive results detected by each test differed slightly, and a combination of the two tests detected the highest number. Counterimmunoelectrophoresis (CIE) was shown to be a very sensitive method for detecting precipitins, but not for their measurement. A prospective evaluation of immunodiffusion, latex agglutination and CIE as potential serodiagnostic techniques for farmer's lung disease is suggested.

MO432COMPARATIVE ANALYSIS OF INDIRECT IMMUNOFLUORESCENCE AND ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR ANTI-PLA2R ASSESMENT IN PATIENTS WITH PRIMARY MEMBRANOUS NEPHROPATHY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Olga Galkina ◽  
Evdokia Bogdanova ◽  
Irina Zubina ◽  
Elena Levykina ◽  
Alexei Smirnov
Keyword(s):  
Indirect Immunofluorescence ◽  
Membranous Nephropathy ◽  
Laboratory Diagnosis ◽  
Response To Therapy ◽  
Control Group ◽  
Course Of Disease ◽  
Phospholipase A2 Receptor ◽  
Gender And Age ◽  
Positive Results

Abstract Background and Aims Antibodies to M-type phospholipase A2 receptor (PLA2R-Ab) are considered to be a promising biomarker for laboratory diagnosis of primary membranous nephropathy (PMN) and may be useful in the evaluation of the response to therapy and CKD prognosis. The aim of the study was to compare two immunoassay methods – indirect immunofluorescence (IIF) and enzyme immunoassay (ELISA) for the determination of circulating PLA2R-Ab in patients with PMN. Method The study included 54 patients aged 55 (40-63) yrs. (M: F [33:21]) with PMN before treatment (n=16) and treated with immunosuppressive therapy (IST) (n=38), and apparently healthy individuals of the corresponding gender and age (n=10). Proteinuria and estimated glomerular filtration rate (eGFR) were determined in all participants. The levels of PLA2R-Ab were determined by IIF and quantitative/ semi-quantitative ELISA (EURUIMMUN AG test, Germany). In 16 PMN patients without treatment and 28 PMN patients treated with IST the level of PLA2R-Ab was measured one time and in 10 PMN patients treated IST – in dynamic, from 2 to 5 times. Statistical comparisons among groups were performed using Mann–Whitney U-test and Kruskal-Wallis H tests. The association between variables was estimated using Spearman’s coefficient. Sensitivity and specificity of the methods were calculated. Results The correlation coefficient between IIF and ELISA was 0.82 (p &lt;0.005). There were more PLA2R-Ab-positive cases detected by ELISA, both before treatment (ELISA - 80%, IIF - 67%) and among patients treated with IST (ELISA - 63%, IIF - 50%). In control group, ELISA showed no positive results for PLA2R-Ab (specificity was 100%). The levels of proteinuria and eGFR were associated with autoantibodies determined by ELISA, both quantitative and semi-quantitative (proteinuria: r = 0.69, p = 0.001; eGFR: r = -0.38, p = 0.035) but not by IIF (proteinuria: r=0.33, p=0.061; eGFR: r=-0.26, p=0.082). The levels of PLA2R-Ab measured by ELISA correlated with the course of disease in patients treated with IST, while IIF did not show any dynamics is some cases. Conclusion Both quantitative and semi-quantitative ELISA were considered to be more preferable methods since the obtained results correlate with renal dysfunction and allow to assess the concentration of PLA2R-Ab in the course of disease more accurately, that may contribute to timely correction of treatment and improvement of outcome.

scholarly journals Impact of Gender and Age on Hyperthermia-Induced Changes in Respiration of Liver Mitochondria

Medicina ◽  
2018 ◽  
Vol 54 (4) ◽  
pp. 62 ◽  
Author(s):  
Giedrė Šilkūnienė ◽  
Rasa Žūkienė ◽  
Zita Naučienė ◽  
Laima Degutytė-Fomins ◽  
Vida Mildažienė
Keyword(s):  
Thermal Inactivation ◽  
Liver Mitochondria ◽  
Female Rats ◽  
Ros Generation ◽  
Age Group ◽  
Male And Female Rats ◽  
Gender And Age ◽  
Induced Changes ◽  
Gender Dependence ◽  
Malate Oxidation

Aim: This study aimed to compare hyperthermia-induced changes in respiration and generation of reactive oxygen species (ROS) in liver mitochondria derived from animals of different gender and age. Methods: The effects of hyperthermia (40–47 °C) on oxidation of different substrates and ROS production were estimated in mitochondria isolated from the liver of male and female rats of the 1–1.5, 3–4, or 6–7 months age. Results: Gender-dependent differences in response of respiration to hyperthermia were the highest at 3–4 months of age, less so at 6–7 months of age, and only minor at juvenile age. Mild hyperthermia (40–42 °C) stimulated pyruvate + malate oxidation in mitochondria of females, but inhibited in mitochondria of males in the 3–4 month age group. The resistance of mitochondrial membrane to hyperthermia was the highest at 3–4 month males, and the lowest in the 6–7 month age group. Inhibition of glutamate + malate oxidation by hyperthermia was caused by thermal inactivation of glutamate dehydrogenase. ROS generation at 37 °C was higher at 1–1.5 month of age, but the increase in ROS generation with rise in temperature in this age group was the smallest, and the strongest in 6–7 month old animals of both genders. Conclusions: The response to hyperthermia varies during the first 6–7 months of life of experimental animals: stronger gender dependence is characteristic at 3–4 months of age, while mitochondria from 6–7 months animals are less resistant to hyperthermia.

scholarly journals Epidemiological situation of bovine brucellosis in the State of Paraiba, Brazil

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3403 ◽  
Author(s):  
Inácio José Clementino ◽  
Ricardo Augusto Dias ◽  
Marcos Amaku ◽  
Fernando Ferreira ◽  
Evelise Oliveira Telles ◽  
...  
Keyword(s):  
Risk Factor ◽  
Odds Ratio ◽  
The State ◽  
Prevalence Rates ◽  
Risk Of Infection ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
The Rose ◽  
Positive Results ◽  
Epidemiological Situation

This study was performed to characterize the epidemiological status of brucellosis in the State of Paraíba, Brazil. The State was divided into three regions. Herds were randomly sampled in each region and a pre-established number of animals were sampled in each of these herds. A total of 3,489 serum samples from 674 herds were collected. In each herd, an epidemiological questionnaire was conducted. This questionnaire focused on herd traits, as well as husbandry and sanitary practices that could be associated with the risk of infection. The serum samples were screened for antibodies against Brucella spp. by the Rose-Bengal Test (RBT), and all positive sera were confirmed by the 2-mercaptoethanol test (2-ME). The herd was considered positive if at least one animal had positive results for both the RBT and the 2-ME test. The prevalence rates of infected herds and animals in the State were 4.6% [3.2-6.5%] and 2.5% [1.1-3.9%], respectively. The prevalence rates of infected herds and animals in the regions were, respectively: region 1, 3.2% [1.5-6.6%] and 1.7% [0.5-5.7%]; region 2, 2.2% [0.9-5.2%] and 0.7% [0.3-1.7%]; and region 3, 7.9% [5.0-12.2%] and 3.2% [1.6-6.3%]. The risk factor (odds ratio, OR) associated with the presence of the infection was Zebuine as the predominant breed (OR=12.30 [1.32-114.64]).

scholarly journals Etiological profile, gender difference and age group patterns of 415 patients presenting with lower gastrointestinal bleeding in the western region of Nepal

2015 ◽  
Vol 3 (2) ◽  
pp. 52-55
Author(s):  
Umid Kumar Shrestha
Keyword(s):  
Colon Cancer ◽  
Gender Difference ◽  
Gastrointestinal Bleeding ◽  
Lower Gastrointestinal Bleeding ◽  
Colon Polyp ◽  
Colon Polyps ◽  
Age Group ◽  
Gender And Age ◽  
Common Causes ◽  
Radiation Colitis

Background: Lower gastrointestinal bleeding (LGIB) is a common clinical condition associated with significant morbidity and mortality. The aims of our study were to determine the etiological profile, gender difference and age group patterns of LGIB in Nepal.Methods: A total of 415 consecutive patients presenting with LGIB from April 2011 to September 2014 underwent colonoscopy and the etiology of LGIB was determined. Each diagnosis was compared with respect to the gender and age group.Results: Among 415 LGIB patients (males 62.2%, females 37.8%), the different etiologies of LGIB were as following: hemorrhoid 35.2%, non-specific colitis 24.8%, colon polyp 18.3%, inflammatory bowel disease (IBD) 10.4%, colon cancer 6.5%, diverticulosis 1.7%, unknown 1.4%, upper gastrointestinal bleeding 1.2% and radiation colitis 0.5%. The overall LGIB was more common in male; however, colon polyps were diagnosed more commonly in female (p<0.001). Among LGIB patients, the hemorrhoid was more common in the age group of 30-49 years, colon polyp in 50-69 years, colon cancer in 70-84 years, and non-specific colitis and IBD in 16-29 years (p<0.001).Conclusion: Hemorrhoid and non-specific colitis were the common causes of LGIB in Nepal; colon polyp, IBD and colon cancer were the next common causes. More males presented with LGIB than females; however, colon polyp was diagnosed more in females, which needs to be explored further. When LGIB was presented after 50 years of age, the most likely causes were colon polyp and colon cancer; hence colonoscopy is recommended in all LGIB patients after 50 years.Journal of Advances in Internal Medicine 2014;3(2):52-55

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