Diagnostic system of determination of an acute cholangitis

Objecive. To elaborate a diagnostic system, permitting to confirm or exclude the diagnosis of an acute cholangitis in patients, suffering biliary ducts obstruction. Materials and methods. The wok is based on analysis of clinical, laboratory and instrumental indices in 174 patients, suffering the biliary ducts obstruction (in 18 - with an acute cholangitis). Results. The diagnostic system was constructed, taking into account informativity of such indices, as ratio of the segmented and stab neutrophils quantity, quantity of stab neutrophils, bilirubin, a gallbladder wall thickness, quantity of monocytes, leukocytes, the Charcot’s triad presence, the body temperature, level of amylase, the duodenal papilla magna size, quantity of lymphocytes, presence of excluded gallbladder, presence of cholecystectomy in anamnesis, the immobile calculus of duodenal papilla magna, tumor, the eosinophils quantity, as well as the presence of any choledocholithiasis and age. Conclusion. The elaborated diagnostic scheme for determination of an acute cholangitis in patients, suffering biliary ducts obstruction, owes high security (≥ 95%), because the part of failed diagnosis did not exceeded 5% and have constituted 2.8%.

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MULTISENSORY LEARNING SYSTEM FOR NON-INVASIVE DETERMINATION OF THE FUNCTIONAL STATE OF THE BODY IN EXTREME CONDITIONS

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES ◽

10.37747/2312-640x-2020-18-176-179 ◽

2020 ◽

pp. 176-179

Author(s):

A. Zaitseva ◽

Y. Kislyakov ◽

S. Avduschenko

Keyword(s):

Diagnostic System ◽

The Body ◽

Learning System ◽

Extreme Conditions ◽

Non Invasive ◽

Metabolic Products ◽

Multisensory Learning ◽

Analytical System ◽

Invasive Diagnostics

The project is implementing a new principle of non-invasive diagnostics and biomedical research using an artificial, multi-sensory, trained analytical system to monitor the professional fitness of military personnel in terms of exhaled air condensate, in which significant metabolic products are present. The results of the studies show that the subjects are divided into groups of different adaptability and sensitivity to extreme conditions. The proposed method based on the multi-sensory trained diagnostic system allows non-invasive instrumental control and selection of spe- cialists for their functioning in extreme conditions of high mountains.

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Contactless diagnostics of the technical state of the valve mechanism of the internal combustion engine

Системи озброєння і військова техніка ◽

10.30748/soivt.2020.64.06 ◽

2020 ◽

pp. 49-53

Author(s):

В.В. Войтов ◽

Ю.В. Чепурний ◽

А.В. Статигін

Keyword(s):

Acoustic Noise ◽

Combustion Engine ◽

Diagnostic System ◽

Acoustic Vibration ◽

The Body ◽

Technical State ◽

Valve Mechanism ◽

Directional Microphone ◽

Diagnostics System

The aim of the study is to analyze the possibilities of developing a contactless diagnostics system of the technical state of the valve mechanism, which excludes mounting sensors on the body of the diagnosed equipment, by using a directional microphone as a sensitive element. In order to test the proposed hypothesis, a directional microphone designed to measure the level of acoustic noise, sound pressure level and to obtain amplitude-frequency characteristics in the range of 10-10 kHz has been used. In the work, an experimental determination of the spectrum of the valve mechanism acoustic vibration and its comparison with the spectrum obtained from an accelerometer has been carried out; the principle of building an automated diagnostic system using a directional microphone has been formulated.

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Gender determination of caucasoid hair using image analysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100146928 ◽

1993 ◽

Vol 51 ◽

pp. 216-217

Author(s):

T.B. Ball ◽

W.M. Hess

Keyword(s):

Image Analysis ◽

Cross Sections ◽

Scalp Hair ◽

The Body ◽

Equivalent Diameter ◽

Cross Sectional ◽

Hair Samples ◽

Length Width ◽

Morphological Differences

It has been demonstrated that cross sections of bundles of hair can be effectively studied using image analysis. These studies can help to elucidate morphological differences of hair from one region of the body to another. The purpose of the present investigation was to use image analysis to determine whether morphological differences could be demonstrated between male and female human Caucasian terminal scalp hair.Hair samples were taken from the back of the head from 18 caucasoid males and 13 caucasoid females (Figs. 1-2). Bundles of 50 hairs were processed for cross-sectional examination and then analyzed using Prism Image Analysis software on a Macintosh llci computer. Twenty morphological parameters of size and shape were evaluated for each hair cross-section. The size parameters evaluated were area, convex area, perimeter, convex perimeter, length, breadth, fiber length, width, equivalent diameter, and inscribed radius. The shape parameters considered were formfactor, roundness, convexity, solidity, compactness, aspect ratio, elongation, curl, and fractal dimension.

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DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

International Journal of Clinical and Biomedical Research ◽

10.31878/ijcbr.2019.54.03 ◽

2019 ◽

pp. 10-13

Author(s):

Ranu Kumar ◽

Prasad Kapildeo

Keyword(s):

Human Blood ◽

Blood Cells ◽

Blood Smear ◽

Clinical Laboratory ◽

White Blood Cells ◽

Healthcare Services ◽

Field Level ◽

Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

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Determination of Lead Content in Red Colored Lipsticks from Mandalay Market by Flame Atomic Absorption Spectrophotometer

Myanmar Health Sciences Research Journal ◽

10.34299/mhsrj.30.03.0045 ◽

2018 ◽

Vol 30 (3) ◽

pp. 167-173

Keyword(s):

Sampling Procedure ◽

The Body ◽

Lead Content ◽

Social Psychological ◽

Allowable Limit ◽

Flame Atomic Absorption ◽

Therapeutic Benefits ◽

Flame Atomic Absorption Spectrophotometer ◽

Exposure Levels

Red colored lipstick is the most widely used cosmetic product. Although lipstick gives a lot of social, psychological and therapeutic benefits, it may harm the consumers. Because some lipsticks contain a considerable amount of heavy metal especially lead. Lead is being used in lipstick mainly for the pigments required to obtain needed colors. Lead accumulates in the body over time and lead-containing lipstick applied several times a day, every day, combined with lead in water and other sources, could add up to significant exposure levels. Therefore, this study was aimed to determine lead content in red colored lipsticks from market. This study was laboratorybased, analytical study by using 25 lipstick samples. Red colored lipsticks were bought from Mandalay Market by random sampling procedure and they were completely coded to avoid the bias. Then, lead content in coded samples was determined by Flame AAS according to International Conference on Harmonization (ICH) guideline. Lead contents of 88% of the lipsticks samples were more than specified limit (20 ppm) of Food and Drug Administration, United States. All of them, lead content was highest in counterfeit lipsticks group. Among the tested lipstick samples, lipstick with lowest lead content was LE-RL 01 (15.74 ppm) and the lipstick with highest lead content was CF-RL 01(60.09 ppm). In conclusion, lead contents of red colored lipsticks (22 out of 25) from market samples were higher than allowable limit (20 ppm).

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Fast Method for the Determination of Residual Solvents in Radiopharmaceutical Products

Revista de Chimie ◽

10.37358/rc.17.4.5526 ◽

2017 ◽

Vol 68 (4) ◽

pp. 666-670 ◽

Cited By ~ 1

Author(s):

Mirela Mihon ◽

Catalin Stelian Tuta ◽

Alina Catrinel Ion ◽

Dana Niculae ◽

Vasile Lavric

Keyword(s):

Gas Chromatography ◽

Control Method ◽

Limit Of Detection ◽

The Body ◽

Biologically Active ◽

Injection Technique ◽

Fast Method ◽

Residual Solvent ◽

Residual Solvents

The aim of this work was the development and validation of a fast analytical method to determine the residual solvents content in radiopharmaceuticals such as: 18F-Fluorodeoxyglucose (18F-FDG), 18F-Fluoroestradiol (18F-FES), 18F-Fluorothymidine (18F-FLT),18F-Fluoromisonidazole (18F-FMISO). Radiopharmaceuticals are radioactive preparations for medical purposes used in nuclear medicine as tracers in diagnostic imaging and treatment of certain diseases. Positron Emission Tomography (PET) is a medical imaging technique that consists in introducing into the body of a small amount of a biologically active chemical compound labelled with a short lived positron-emitting radioisotope (18F, 11C, 68Ga). Residual solvents are critical impurities in radiopharmaceuticals that can affect labelling, stability and physicochemical properties of drugs. Therefore, the determination of these solvents is essential for quality control of radiopharmaceuticals. Validation of the control method for residual solvents by gas chromatography is referred by the European Pharmacopoeia using a special injection technique (head space). The parameters of the method, which comply with International Conference on Harmonization guidelines, are: accuracy, precision, linearity, limit of detection, limit of quantification and robustness. The proposed method (direct gas chromatography injection) proved to be linear, precise, accurate and robust. Good linearity was achieved for all the solvents and correlation coefficients (R2) for each residual solvent were found more than 0.99.

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A Case of Neurosarcoidosis Mimicking Brain Tumor

Current Medical Imaging Formerly Current Medical Imaging Reviews ◽

10.2174/1573405616666201118114152 ◽

2020 ◽

Vol 16 ◽

Author(s):

Lutfullah Sari ◽

Abdusselim Adil Peker ◽

Dilek Hacer Cesme ◽

Alpay Alkan

Keyword(s):

Brain Tumor ◽

Contrast Enhancement ◽

Clinical Laboratory ◽

Vasogenic Edema ◽

Pulmonary Sarcoidosis ◽

Brain Parenchyma ◽

The Body ◽

Oligoclonal Bands ◽

Flair Sequence ◽

History Of

Background: Neurosarcoidosis manifests symptomatically in 5% of patients with sarcoidosis and diagnosis can be challenging if not clinically suspected. Cerebral mass-like presentation of neurosarcoidosis rarely reported in the literature. We presented a woman with neurosarcoidosis who had a cerebral mass-like lesion which completely disappeared after medical treatment. Discussion: A 37-year-old woman with history of pulmonary sarcoidosis referred to the emergency service of our hospital with a one-month history of progressive dizziness, nausea and seeing flashing lights. At neurologic examination, numbness and weakness on the left side of the body, deviation of uvula toward the right side was seen. Cranial MRI demonstrated a 2.5x2 cm in size mass lesion which hypointense on T1 WI, heterogeneous hyperintense on T2 and FLAIR sequence with peripheral vasogenic edema and heterogeneous, irregular contrast enhancement simulating brain tumor. Also, leptomeningeal and nodular contrast enhancement was seen on brainstem, cerebellar vermis, perimesencephalic cistern and left frontal, bilateral parietooccipital sulcus. In laboratory tests; The level of serum angiotensin-converting enzyme (ACE) was 53 IU/mL (N:8-52 IU/mL) and cerebrospinal fluid (CSF) ACE was 23 IU/mL (N:0-2.6 IU/mL). CSF cytology analysis was normal. Pattern 2 oligoclonal bands were present. With these clinical, laboratory and radiological findings, cerebral involvement of sarcoidosis was suspected. Biopsy was not performed due to the high risk of morbidity caused by the deep location of the lesion.Patient was treated with methylprednisolone and Azathioprine for a month.On post-treatment control imaging; lesion disappeared completely without residual leptomeningeal and nodular contrast enhancement.Also, neurologic symptoms were decreased remarkably. Conclusion: Multi-system inflammatory disorders like sarcoidosis, can present with mass-like lesion in the brain parenchyma. While early diagnosis is important to prevent unnecessary interventions like biopsy and surgery, it is crucial to initiate the necessary treatment with the aim of recovery without sequelae. Radiological and clinical follow-up are fundamental in differential diagnosis.

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The pathway through LC-MS method development: in-house or ready-to-use kit-based methods?

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2019-0916 ◽

2020 ◽

Vol 58 (6) ◽

pp. 1002-1009 ◽

Cited By ~ 4

Author(s):

Caroline Le Goff ◽

Jordi Farre-Segura ◽

Violeta Stojkovic ◽

Patrice Dufour ◽

Stéphanie Peeters ◽

...

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Method Development ◽

Clinical Laboratory ◽

Cross Reactivity ◽

Tandem Mass ◽

Chromatography Tandem Mass Spectrometry ◽

Liquid Chromatography Tandem Mass

AbstractHistorically, the determination of low concentration analytes was initially made possible by the development of rapid and easy-to-perform immunoassays (IAs). Unfortunately, typical problems inherent to IA technologies rapidly appeared (e.g. elevated cost, cross-reactivity, lot-to-lot variability, etc.). In turn, liquid chromatography tandem mass spectrometry (LC-MS/MS) methods are sensitive and specific enough for such analyses. Therefore, they would seem to be the most promising candidates to replace IAs. There are two main choices when implementing a new LC-MS/MS method in a clinical laboratory: (1) Developing an in-house method or (2) purchasing ready-to-use kits. In this paper, we discuss some of the respective advantages, disadvantages and mandatory requirements of each choice. Additionally, we also share our experiences when developing an in-house method for cortisol determination and the implementation of an “ready-to-use” (RTU) kit for steroids analysis.

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