PROOXIDANT AND ANTIOXIDANT BLOOD SYSTEMS AND CYTOKINE PROFILE IN PATIENTS WITH ABDOMINAL SEPSIS

2020 ◽  
pp. 113-118
Author(s):  
Miziev I.A. ◽  
Akhkubekov R.A. ◽  
Ivanova Z.O.
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Abdominal Sepsis ◽  
The Body ◽  
Simultaneous Increase ◽  
Inclusion Criteria ◽  
Blood Cytokines ◽  
Nbt Test ◽  
Excessive Entry ◽  
Plasma Antioxidant

Aim. to study the dynamics of clinical and laboratory parameters of endogenous intoxication and prooxidant and antioxidant blood systems in patients with abdominal sepsis. Material and methods. 57 patients with abdominal sepsis were examined. The inclusion criteria for the study were the presence of a clinic of abdominal sepsis and changes in the parameters of the prooxidant and antioxidant blood systems and blood cytokines. Results. In patients with abdominal sepsis, signs of oxidative damage to lipids and proteins were found, as evidenced by a significant increase in the MDA level up to 5.8 μmol / L, catalase content in erythrocytes 94 mmol / min*gHb, spontaneous NBT test activity up to 95% and a decrease in ceruloplasmin in the blood up to 194 mg / l. With abdominal sepsis, a significant increase in the level of TNF-82.7 pkg/ml, IL-10 to 167.6 pkg/ml, IL-6 to the maximum values in this study up to 286.4 pg/ml and IL to 34 pkg was /ml observed. Conclusion. It was found that in patients with abdominal sepsis there is an increase in lipid peroxidation indicators - malondialdehyde in the blood plasma, the activity of the spontaneous NBT test, depending on the phase of the disease and the presence of complicated forms. Changes in the antioxidant defense of the body in abdominal sepsis are manifested by inhibition of the plasma antioxidant ceruloplasmin with a simultaneous increase in catalase in erythrocytes, depending on the phase of the disease and also on the presence of complicated forms of the disease. As a result of this study, increased production of a complex of both pro-inflammatory and anti-inflammatory cytokines with their rapid and excessive entry into the peripheral blood was revealed.

scholarly journals INFLUENCE OF «METISEVIT» ON THE ACTIVITY OF ENZYME AND NONENZYME LINK OF ANTIOXIDANT PROTECTION UNDER THE BULL’S BODY CADMIUM LOADING

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 52-59
Author(s):  
B.V. Gutyj ◽  
Y. Lavryshyn ◽  
V. Binkevych ◽  
O. Binkevych ◽  
О. Paladischuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Cadmium Stress ◽  
Antioxidant Defense System ◽  
The Body ◽  
Antioxidant Protection ◽  
Young Cattle

The article contains the research results of the effect of cadmium chloride on the indexes of enzyme and nonenzyme systems of  antioxidant defense system in young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E. It is established that feeding calves at a dose of toxicant 0.04 mg / kg activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E in the blood of experimental animals decreased throughout the experiment. The lowest indicators of antioxidant in the blood of young cattle is set on the twenty -fourth day of the experiment, which is associated with increased activation of lipid peroxidation and the balance between antioxidant system and lipid peroxidation intensity. Given the cadmium load of young cattle it is used a new integrated drug with antioxidant action «Metisevit», which includes metifen, sodium selenite and vitamin E wich is founded as stimulating effects on the activity of antioxidant protection. In particular,it is established probable increase in activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, vitamin A and vitamin E in the blood of young cattle, which has performed cadmium stress. These changes occur through comprehensive action components of the drug «Metisevit» that leads to the normalization of metabolic processes and free radical in the body of the bull. The results of the research indicate antioxidant drug «Metisevit» in the application of its young cattle and the validity of his administration to improve the body's antioxidant status of chronic cadmium toxicosis.

scholarly journals L-glutamine ameliorates adipose-hepatic dysmetabolism in OC-treated female rats

2020 ◽  
Vol 246 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kehinde Samuel Olaniyi ◽  
Lawrence Aderemi Olatunji
Keyword(s):  
Lipid Peroxidation ◽  
Uric Acid ◽  
Antioxidant Defense ◽  
Glycogen Synthesis ◽  
Lactate Production ◽  
The Body ◽  
Female Rats ◽  
Hepatic Lipid ◽  
Glutamyl Transferase ◽  
Adipose Dysfunction

Adipose dysfunction and inflammation with or without hepatic defects underlie metabolic obesity. Glutamine (GLU) improves glucoregulation and metabolic indices but its effects on adipose function and hepatic lipid deposition in estrogen-progestin oral contraceptive (EPOC) users are unknown. Therefore, we hypothesized that GLUT supplementation would protect against adipose dysfunction and excess hepatic lipid influx and deposition in EPOC-treated animals by suppressing adenosine deaminase/xanthine oxidase (ADA/XO) activity and improving glucose-6-phosphate dehydrogenase (G6PD)-dependent antioxidant defense. Female Wistar rats weighing 150–180 g were allotted into control, GLUT, EPOC and EPOC + GLUT groups (six rats/group). The groups received vehicle (distilled water, p.o.), GLUT (1 g/kg), EPOC containing 1.0 µg ethinylestradiol plus 5.0 µg levonorgestrel and EPOC plus GLUT, respectively, daily for 8 weeks. Results showed that the administration of EPOC caused glucose dysregulation and increased triglyceride-glucose index and visceral adiposity, but the body weight and liver weight were not affected. However, EPOC significantly decreased adipose lipid, G6PD and glutathione and increased glycogen synthesis, ADA, XO, uric acid, lipid peroxidation, lactate production and gamma-glutamyl transferase activity (GGT). On the other hand, EPOC increased hepatic lipid, ADA, XO, uric acid, lipid peroxidation and lactate production and decreased glycogen synthesis, G6PD and glutathione. Nevertheless, supplementation with glutamine attenuated these alterations. Collectively, the present results indicate that EPOC causes metabolically induced obesity which is associated with adipose dysfunction and hepatic metabolic disturbance. The findings also suggest that glutamine confers metabo-protection with corresponding improvement in adipose and hepatic metabolic function by suppression of ADA/XO activity and enhancement of G6PD-dependent antioxidant defense.

scholarly journals State of antioxidant protection system in carp hepatopancrease affected by associative Aeromonas Hydrophila infection with Saprolegnia Sp.

2020 ◽  
pp. 85-89
Author(s):  
Kh. Ya. Solopova ◽  
◽  
O. I. Vishchur ◽  
M. Z. Korylyak ◽  
I. Ye. Solovodzinska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Aeromonas Hydrophila ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Defense System ◽  
Peroxidation Processes ◽  
Experimental Group

Bacterial diseases are responsible for heavy mortality in both wild and cultured fish. Co-infections are very common in nature and occur when hosts are infected by two or more different pathogens either by simultaneous or secondary infections so that two or more infectious agents are active together in the same host. Associative infections pose a significant threat to fish farming, so it is necessary to develop effective means of prevention and treatment of these infections; it is advisable to study in detail their impact on various fish. The aim of the study was to determine the intensity of lipid peroxidation processes and the activity of the antioxidant defense system in the body of carp suffering from aeromonosis and the associated infection of aeromonosis with saprolegniosis. The research was conducted at the Lviv Research Station of the Institute of Fisheries of NAAS. There were three groups of fish, 5 in each. The control group consisted of clinically healthy fish, the first experimental group (D1) – carps affected by Aeromonas hydrophila infection, the second experimental group (D2), which consisted of carps affected by associative Aeromonas hydrophila infection with Saprolegnia Sp. The results of studies have shown that carp with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. leads to the activation of lipid peroxidation processes, especially the primary product of LPO. Thus, in the hepatopancreas of fish there was an increase of 1.4 (p <0.05) in the reasonable content of diene conjugates and there was a tendency to increase the content of TBA-active products. At the same time, the inhibitory effect of pathogenic factors of this disease on the activity of the enzymatic link of the antioxidant defense system was stated. In particular, carp patients with associative infection ‒ of Aeromonas hydrophila infection with Saprolegnia Sp. had higher catalase and superoxide dismutase activity, respectively, 1.3 (p <0.05) and 2.3 (p <0.01) times. The results of experimental studies made it possible to expand and deepen the mechanisms of the pathogenesis of the co-infection of Aeromonas hydrophila infection with Saprolegnia Sp. and to use the data to develop effective treatments.

scholarly journals Typical relationships between the indexes of lipid peroxidation/ antioxidant protection and immune regulation in the patients with chronic atrophic gastritis and stomach cancer

2020 ◽  
Vol 22 (5) ◽  
pp. 897-906
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Atrophic Gastritis ◽  
Normal Distribution ◽  
Antioxidant Defense ◽  
Malonic Dialdehyde ◽  
Chronic Atrophic Gastritis ◽  
The Body ◽  
Control Group ◽  
Antioxidant Protection

Chronic atrophic gastritis and gastric cancer represent distinct steps of one pathogenic process. The risk of developing cancer of the stomach is directly proportional to the degree of atrophic changes simultaneously detected in antral segment and in the body of the stomach. The role of immune system in transformation of precancerous diseases into cancer is beyond doubt. During development of the malignant disease, the changes in lipid peroxidation systems – antioxidant defense become significant and contribute to the progression of the tumor and the development of metastases. A simultaneous study of lipid peroxidation and antioxidant defense indices along with phagocytic activity will allow us to evaluate relative contribution of these processes to development of chronic atrophic gastritis and gastric cancer. Purpose of the present study was to assess correlations between the lipid peroxidation indices, i.e. antioxidant protection, and chemiluminescent activity of neutrophilic granulocytes and monocytes in chronic atrophic gastritis and gastric cancer. Forty patients with chronic gastritis, 22 patients with chronic atrophic gastritis and 40 patients with gastric cancer were examined. The control group consisted of 50 practically healthy age-matched volunteers. Evaluation of spontaneous and induced production of reactive oxygen species by neutrophils and monocytes was carried out by chemiluminescent analysis. The parameters of lipid peroxidation/ antioxidant protection were determined by spectrophotometric methods. Statistical data processing was carried out using the Statistica v. 8.0 program (StatSoft Inc., USA). The normal distribution of indices was tested using the Kolmogorov–Smirnov method (adjusted by Lillefors). Quantitative indicators, given the normal distribution, were described using the median (Me) and interquartile scatter (Q0.25-Q0.75). To study statistical significance of differences between quantitative characteristics, the Mann–Whitney test was used. To study strength of relationships of these indicators, the Pearson rank correlation coefficient (r) was calculated. The critical significance level (p) when testing statistical hypotheses was taken equal to 0.05. Correlation analysis showed that the weight of positive correlations increases in patients with chronic atrophic gastritis, and it decreases in patients with gastric cancer, the strength of the correlation dependence and new relationships appear between chemiluminescent activity of neutrophils and monocytes in a spontaneous and induced state, and the amounts of malonic dialdehyde, enzyme activities of superoxide dismutase and catalase. In chronic atrophic gastritis and gastric cancer we have established the features of correlation patterns between lipid peroxidation/antioxidant protection indices, and activity of neutrophils and monocytes.

POSSIBILITIES OF ANTIOXIDANT PROTECTION IN PATIENTS WITH SUPERIOR HYPERPLASIA OF THE PEDESTAL GLAND AND ACCOMPANYING CHRONIC POSTATITIS

2020 ◽  
pp. 8-11
Author(s):  
Павел Георгиевич Осипов ◽  
Андрей Александрович Береш ◽  
Юрий Сергеевич Ханин ◽  
Олеся Игоревна Некрылова
Keyword(s):  
Lipid Peroxidation ◽  
Chronic Prostatitis ◽  
Antioxidant Defense ◽  
Prostate Gland ◽  
Antioxidant Defense System ◽  
Complex Treatment ◽  
Traditional Treatment ◽  
Important Place ◽  
Antioxidant Defense Systems ◽  
Benign Hyperplasia

Несмотря на достигнутые успехи в диагностике и лечении, на сегодняшний день проблема хронического простатита у пациентов с доброкачественной гиперплазией простаты продолжает оставаться актуальной. Тем временем, выздоровление или же стойкая ремиссия хронического простатита наступает только у 30-35% больных, которые получают традиционное лечение. В патогенезе хронического простатита важное место занимают мембранопатологические процессы, которые обусловлены активацией перекисного окисления липидов и нарушением состояния антиоксидантной системы защиты. У больных с хроническим простатитом и доброкачественной гиперплазией простаты наблюдается существенное усиление процессов липопероксидации на фоне сниженной функциональной способности антиоксидантных систем защиты организма. Поэтому равновесие в оксидантно-антиоксидантной системе является важным звеном в поддержании гомеостаза и, в частности, при патологии предстательной железы, предопределяет включение в комплексное лечение средств антиоксидантного действия. Перспективным можно считать применение препаратов с высоким содержанием биофлавоноидов и антиоксидантных витаминов. Включение в комплексное лечение таких пациентов препарата Кверцетина приводит к сокращению сроков нормализации клинико-лабораторных проявлений заболевания благодаря восстановлению равновесия между перекисным окислением липидов и состоянием антиоксидантной системы защиты Despite the successes achieved in the diagnosis and treatment, to date, the problem of chronic progression in patients with benign hyperplasia of the growth continues to remain relevant. Meanwhile, recovery or persistent remission of chronic prostatitis occurs only in 30-35% of patients who receive traditional treatment. In the pathogenesis of chronic prostatitis, membrane-pathological processes take an important place, which are caused by the activation of lipid peroxidation and impaired state of the antioxidant defense system. In patients with chronic prostate and benign hyperplasia, a significant increase in lipoperoxidation processes is observed against the background of a reduced functional ability of antioxidant defense systems. Therefore, the equilibrium in the oxidant-antioxidant system is an important link in the maintenance of homeostasis and, in particular, with the pathology of the prostate gland, allows the inclusion of antioxidant drugs in the complex treatment. The use of drugs with a high content of bioflavonoids and antioxidant vitamins can be considered promising. The inclusion of such patients in the complex treatment of the drug Quercetin leads to a reduction in the normalization period of the clinical and laboratory manifestations of the disease due to the restoration of the equilibrium between the peroxidation of the lipid peroxidation system and the state of lipid peroxidation

Pathogenetic Therapy of Patients with Recurrent Genital Herpes

2020 ◽  
Vol 65 (5-6) ◽  
pp. 30-34
Author(s):  
L. L. Logvina ◽  
D. N. Bayram ◽  
Z. A. Kambachokova ◽  
F. V. Shavaeva ◽  
Z. S. Krymshokalova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Plasma ◽  
Genital Herpes ◽  
Antioxidant Defense ◽  
Clinical Manifestations ◽  
Complex Treatment ◽  
Herpesvirus Infection ◽  
Recurrent Genital Herpes ◽  
Complex Therapy ◽  
Pathogenetic Therapy

Evaluation of the effectiveness of aminophthalhydroside in the complex treatment of patients with recurrent genital herpes was carried out. The efficacy of the drug was assessed by clinical, biochemical, and immunological criteria. The inclusion of aminophthalhydrazide in the complex therapy of patients with recurrent herpesvirus infection led to an earlier relief of clinical manifestations of the disease, an increase in the relapse period, a decrease in lipid peroxidation products in the blood plasma, an increase in the content of antioxidant defense components, and correction of immunological disorders.

Mechanisms of Nickel-Induced Cell Damage in Allergic Contact Dermatitis and Nutritional Intervention Strategies

2020 ◽  
Vol 20 (7) ◽  
pp. 1010-1014 ◽  
Author(s):  
Dana Filatova ◽  
Christine Cherpak
Keyword(s):  
Lipid Peroxidation ◽  
Contact Dermatitis ◽  
Oxidative Damage ◽  
Allergic Contact Dermatitis ◽  
Cell Damage ◽  
Consumer Products ◽  
The Body ◽  
Cellular Damage ◽  
Nutritional Interventions ◽  
Allergic Contact

Background: Hypersensitivity to nickel is a very common cause of allergic contact dermatitis since this metal is largely present in industrial and consumer products as well as in some commonly consumed foods, air, soil, and water. In nickel-sensitized individuals, a cell-mediated delayed hypersensitivity response results in contact to dermatitis due to mucous membranes coming in long-term contact with nickel-containing objects. This process involves the generation of reactive oxidative species and lipid peroxidation-induced oxidative damage. Immunologically, the involvement of T helper (h)-1 and Th-2 cells, as well as the reduced function of T regulatory cells, are of importance. The toxicity, mutagenicity, and carcinogenicity of nickel are attributed to the generation of reactive oxygen species and induction of oxidative damage via lipid peroxidation, which results in DNA damage. Objective: The aim of this research is to identify nutritionally actionable interventions that can intercept nickel-induced cell damage due to their antioxidant capacities. Conclusion: Nutritional interventions may be used to modulate immune dysregulation, thereby intercepting nickel-induced cellular damage. Among these nutritional interventions are a low-nickel diet and an antioxidant-rich diet that is sufficient in iron needed to minimize nickel absorption. These dietary approaches not only reduce the likelihood of nickel toxicity by minimizing nickel exposure but also help prevent oxidative damage by supplying the body with antioxidants that neutralize free radicals.

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