PHOTODYNAMIC THERAPY WITH METHYLENE BLUE IN CHILDREN WITH CEREBRAL PARALYSIS

2021 ◽  
pp. 31-35
Author(s):  
Kashtanova M.S. ◽  
Morozova N.S. ◽  
Aslanova D.R.
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Bacterial Biofilm ◽  
Treatment Technique ◽  
Healthy Children ◽  
Oral Diseases ◽  
Active Forms Of Oxygen ◽  
Periodontal Tissues ◽  
Cerebral Paralysis ◽  
Dental Diseases

Children with cerebral palsy suffer from dental diseases almost three times more often than healthy children. Risk factors that cause the development of dental diseases in children with CP include low rates of unstimulated salivation rate, acidity, increased saliva osmolarity and total protein concentration, which indicates a violation of hydration. The above factors contribute to the formation of acquired pellicles and biofilms due to increased bacterial agglutination. Ultimately, this contributes to the accelerated formation of plaque. There is a tendency to increase the concentration of hemoglobin and reduce the degree of oxygenation of oxyhemoglobin and, as a result, to increase the active forms of oxygen and nitrogen, which, in turn, have a toxic effect and lead to cell death. Photodynamic therapy (PDT) using photosensitizers (FS) improves blood filling and the degree of oxygenation in periodontal tissues, and also allows the elimination of bacterial biofilm. At the same time, opto-spectral diagnostics painlessly in the monitoring mode allows to identify and fix tissue areas with low hemoglobin oxygenation rates for precision photodynamic effects. PDT is a method used as a therapy for oral diseases of various origins, such as cancer, precancerous diseases, caries, periodontitis and gingivitis. Initially, methylene blue was the first dye used in medicine as an antiseptic, as well as the first tested and approved photosensitizer for antimicrobial photodynamic therapy. The use of photodynamic therapy with methylene blue as a photosensitizer can allow to deactivate inflammation and completely destroy bacteria in the biofilm, which will contribute to the functional normalization of gum tissue and restore oxygenation in the microcirculatory bed. The advantages of this method are antibacterial, anti-inflammatory and wound healing effects. The article presents the literature data on photodynamic therapy with the use of methylene blue in children, on the methodology and results of the use of this treatment technique in practice.

scholarly journals Methylene Blue-Mediated Photodynamic Therapy Induces Macrophage Apoptosis via ROS and Reduces Bone Resorption in Periodontitis

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Chunlan Jiang ◽  
Wenyi Yang ◽  
Chengyi Wang ◽  
Wei Qin ◽  
Jiajun Ming ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Bone Resorption ◽  
Laser Irradiation ◽  
Alveolar Bone ◽  
Fluorescence Probe ◽  
Periodontal Tissues ◽  
Macrophage Apoptosis

Aim. To investigate whether methylene blue-mediated photodynamic therapy (MB-PDT) can affect the “fate” of macrophages in vitro or in periodontitis tissues and to explore the potential mechanism. Methods. For in vitro treatments, THP-1 macrophages were divided into three experimental groups: C/control, no treatment; MB, methylene blue treatment; and MB-PDT, MB and laser irradiation treatment. Then, apoptosis and apoptosis-related proteins were detected in each group. For in vivo treatments, periodontitis was ligature-induced in the first molars of the bilateral maxilla in 12 Sprague Dawley (SD) rats. After six weeks, the ligatures were removed and all the induced molars underwent scaling and root planning (SRP). Then, the rats were divided into three groups according to the following treatments: SRP, saline solution; MB, phenothiazinium dye; and MB-PDT, MB and laser irradiation. Apoptotic macrophages, inflammation levels, and alveolar bone resorption in the periodontal tissues of rats were analyzed in each group. Results. In vitro, flow cytometry analysis demonstrated that 10 μM MB and 40 J/cm2 laser irradiation maximized the apoptosis rate (34.74%) in macrophages. Fluorescence probe and Western blot analyses showed that MB-PDT induced macrophage apoptosis via reactive oxygen species (ROS) and the mitochondrial-dependent apoptotic pathway. Conversely, the addition of exogenous antioxidant glutathione (GSH) and the pan-caspase inhibitor Z-VAD-FMK markedly reduced the apoptotic response in macrophages. In vivo, immunohistochemistry, histology, radiographic, and molecular biology experiments revealed fewer infiltrated macrophages, less bone loss, and lower IL-1β and TNF-α levels in the MB-PDT group than in the SRP and MB groups (P<0.05). Immunohistochemistry analysis also detected apoptotic macrophages in the MB-PDT group. Conclusion. MB-PDT could induce macrophage apoptosis in vitro and in rats with periodontitis. This may be another way for MB-PDT to relieve periodontitis in addition to its antimicrobial effect. Meanwhile, MB-PDT induced apoptosis in THP-1 macrophages via the mitochondrial caspase pathway.

scholarly journals Impact of the Diet on the Formation of Oxidative Stress and Inflammation Induced by Bacterial Biofilm in the Oral Cavity

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1372
Author(s):  
Ilona Rowińska ◽  
Adrianna Szyperska-Ślaska ◽  
Piotr Zariczny ◽  
Robert Pasławski ◽  
Karol Kramkowski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oral Cavity ◽  
Pathogenic Bacteria ◽  
Soft Tissues ◽  
Bacterial Flora ◽  
Bacterial Biofilm ◽  
Oral Microbiome ◽  
Diffusion Method ◽  
Oral Diseases ◽  
Periodontal Tissues

The diet is related to the diversity of bacteria in the oral cavity, and the less diverse microbiota of the oral cavity may favor the growth of pathogenic bacteria of all bacterial complexes. Literature data indicate that disturbances in the balance of the bacterial flora of the oral cavity seem to contribute to both oral diseases, including periodontitis, and systemic diseases. If left untreated, periodontitis can damage the gums and alveolar bones. Improper modern eating habits have an impact on the oral microbiome and the gut microbiome, which increase the risk of several chronic diseases, including inflammatory bowel disease, obesity, type 2 diabetes, cardiovascular disease and cancer. The subject of our consideration is the influence of the traditional diet on the formation of oxidative stress and inflammation caused by bacterial biofilm in the oral cavity. Through dental, biomedical and laboratory studies, we wanted to investigate the effect of individual nutrients contained in specific diets on the induction of oxidative stress inducing inflammation of the soft tissues in the oral cavity in the presence of residual supra- and subgingival biofilm. In our research we used different types of diets marked as W, T, B, F and noninvasively collected biological material in the form of bacterial inoculum from volunteers. The analyzed material was grown on complete and selective media against specific strains of all bacterial complexes. Additionally, the zones of growth inhibition were analyzed based on the disc diffusion method. The research was supplemented with dental and periodontological indicators. The research was supplemented by the application of molecular biology methods related to bacterial DNA isolation, PCR reactions and sequencing. Such selected methods constitute an ideal screening test for the analysis of oral bacterial microbiota. The obtained results suggest that certain types of diet can be an effective prophylaxis in the treatment of civilization diseases such as inflammation of the oral cavity along with periodontal tissues and gingival pockets.

scholarly journals Загальні та місцеві фактори ризику виникнення основних стоматологічних захворювань у дітей, хворих на олігофренію

2017 ◽  
Author(s):  
T. D. Zabolotny ◽  
G. Z. Dutko
Keyword(s):  
Gastrointestinal Tract ◽  
Underlying Disease ◽  
Main Group ◽  
Control Group ◽  
Healthy Children ◽  
Local Factor ◽  
Dental Tissues ◽  
Periodontal Tissues ◽  
Dental Diseases ◽  
Local Risk

Prevention of major dental diseases in children is one of the most urgent problems of dentistry in childhood. This is especially true in the category of children with central nervous system (CNS) disorders. It is known that deviations in the psychological development of children are often accompanied by somatic diseases. The literature highlights the dependence of dental status on somatic health.The aim of the study – to conduct an analysis of the somatic status of children with oligophrenic patients.Materials and Methods. The data presented during the examination of 263 children aged 12–18 years. The choice of this age group was subordinated to WHO (1997) requirements to obtain a reliable characterization of the state of solid dental tissues (group of children 12 years old) and periodontal tissues (group of children 15 years). A group of children 18 years old was selected to study the disease of hard tissues of teeth and periodontal tissues in the dynamics. Dental examination of this contingent of children was carried out at the psycho-neurological dispensary of the city of Lviv. The diagnosis of "oligophrenia" was established by a specialist of this specialty (psychiatrist). The control group included 99 students from several secondary schools in Lviv, of the same age, without psychoneurological abnormalities.Results and Discussion. Having conducted a detailed analysis of these indicators among the children of the main group, we have established their direct dependence on the severity of the underlying disease. According to the results of the research, in the main group there is a high percentage of children who were ill with infectious diseases (50 ± 3.9 %), indicating a decrease in the protective immune forces of the organism. Prevalent in the main group of children are diseases of the gastrointestinal tract. A significant number of children with oligophrenia who had a respiratory tract pathology (34.76 ± 3.72 %) were found. In addition to the general factors contributing to the occurrence of pathological processes in the tissues of the oral cavity, there are local, such as: anomalies of bite, dental arches, buckling of teeth, and the like.Conclusions. Some general and local risk factors for dental diseases characteristic of children with oligophrenia have been identified: pathological changes in the respiratory system (34.76 ± 3.72 %) and the gastrointestinal tract (32.32 ± 3.65 %) are more frequent than in mentally healthy children. Oral respiration is on the first place of the local factor of the occurrence of dental diseases in this contingent of children (59.15 ± 3.84 %). Particular weight is occupied by buccal pathology and teething brittleness (40.85 ± 3.84 %). We have also established the dependence of the general state of health of children-oligophrenic patients on the degree of reduction of their intelligence.

scholarly journals MODERN ASPECTS OF THE PROBLEM OF RELATIONSHIP OF DISEASES OF PERIODONTAL AND DIABETES I TYPE IN CHILDREN

2019 ◽  
Vol 19 (4) ◽  
pp. 130-135
Author(s):  
I.O. Kuz ◽  
O.V. Sheshukova
Keyword(s):  
Type I Diabetes ◽  
Periodontal Diseases ◽  
Global Scale ◽  
Type I ◽  
Healthy Children ◽  
Scientific Publications ◽  
Periodontal Tissues ◽  
Dental Diseases ◽  
The One ◽  
Relationship Of

The literature review considers selected modern scientific publications by foreign, Ukrainian and Russian authors regarding pathological changes in the periodontal tissues of children with type I diabetes. Analysis of scientific sources reveals that diabetes is acquiring the characteristics of omnipresent epidemic not only in Ukraine but also in the global scale. The unprecedented increase in the incidence of type I diabetes, even in children under 3 years of age should be taken into account too. It should be borne in mind that diabetes is characterized by a significant prevalence, lifelong course, early disability and high mortality rates. Although there are a number of authors claiming that dental diseases in children with diabetes are not more pronounced than in healthy ones, the vast majority of scientists still acknowledge such interconnection. They emphasize the regenerative ability of the oral mucosa is reduced, periodontal disease is a complication of diabetes and requires appropriate measures in the form of early diagnosis, prevention and treatment. The review provides information on current views on pathogenetic common features for endocrinological diseases and periodontal pathologies, namely: angiopathy (at the level of blood vessels of the microvasculature), metabolic disorders, changes in lipid peroxidation, autoaggression and the occurrence of secondary immunodeficiency. Research data on the nature and degree of indicators’ changes of oral immunity in chronic catarrhal gingivitis in somatically healthy children and children with concomitant somatic pathology are quite contradictory. There exists a need for further review of existing and conduct modern clinical studies related to biochemical processes in saliva and other body fluids, which could clarify the interrelationship between gingivitis, on the one hand, and type I diabetes, on the other. Thus, a deep and comprehensive study of the abovementioned relationship remains expedient, and the development of therapeutic measures in children with inflammatory periodontal diseases is especially relevant.

scholarly journals Applications of Photodynamic Therapy in Management of Periodontal Disease - A Review

2018 ◽  
Vol 08 (04) ◽  
pp. 024-027
Author(s):  
Shiny Inasu ◽  
Biju Thomas
Keyword(s):  
Photodynamic Therapy ◽  
Periodontal Disease ◽  
Infection Control ◽  
Singlet Oxygen ◽  
Current Status ◽  
Target Cells ◽  
Oral Diseases ◽  
Dynamic Therapy ◽  
Reactive Agents ◽  
Photo Dynamic Therapy

AbstractA novel noninvasive photochemical approach for infection control, namely photodynamic therapy, has received much attention in the treatment of oral diseases which requires three nontoxic ingredients namely visible harmless light, a photosensitizer and oxygen are involved in this therapy. It is based on the principle that a photosensitizer binds to the target cells which when activated by light of a suitable wavelength results in the production of singlet oxygen and other very reactive agents that are extremely toxic to certain cells and bacteria. This article highlights the application of photo-dynamic therapy in management of periodontal disease and its current status.

scholarly journals Antimicrobial photodynamic therapy mediated by methylene blue in surfactant vehicle on periodontopathogens

2020 ◽  
Vol 31 ◽  
pp. 101784
Author(s):  
Ana Carolina A.C. Tortamano ◽  
Giuliana G. Anselmo ◽  
Claudio T. Kassa ◽  
Bianca Godoy-Miranda ◽  
Christiane Pavani ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy
Sign in / Sign up

Export Citation Format

Share Document