scholarly journals Impact of the Diet on the Formation of Oxidative Stress and Inflammation Induced by Bacterial Biofilm in the Oral Cavity

Materials ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1372
Author(s):  
Ilona Rowińska ◽  
Adrianna Szyperska-Ślaska ◽  
Piotr Zariczny ◽  
Robert Pasławski ◽  
Karol Kramkowski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oral Cavity ◽  
Pathogenic Bacteria ◽  
Soft Tissues ◽  
Bacterial Flora ◽  
Bacterial Biofilm ◽  
Oral Microbiome ◽  
Diffusion Method ◽  
Oral Diseases ◽  
Periodontal Tissues

The diet is related to the diversity of bacteria in the oral cavity, and the less diverse microbiota of the oral cavity may favor the growth of pathogenic bacteria of all bacterial complexes. Literature data indicate that disturbances in the balance of the bacterial flora of the oral cavity seem to contribute to both oral diseases, including periodontitis, and systemic diseases. If left untreated, periodontitis can damage the gums and alveolar bones. Improper modern eating habits have an impact on the oral microbiome and the gut microbiome, which increase the risk of several chronic diseases, including inflammatory bowel disease, obesity, type 2 diabetes, cardiovascular disease and cancer. The subject of our consideration is the influence of the traditional diet on the formation of oxidative stress and inflammation caused by bacterial biofilm in the oral cavity. Through dental, biomedical and laboratory studies, we wanted to investigate the effect of individual nutrients contained in specific diets on the induction of oxidative stress inducing inflammation of the soft tissues in the oral cavity in the presence of residual supra- and subgingival biofilm. In our research we used different types of diets marked as W, T, B, F and noninvasively collected biological material in the form of bacterial inoculum from volunteers. The analyzed material was grown on complete and selective media against specific strains of all bacterial complexes. Additionally, the zones of growth inhibition were analyzed based on the disc diffusion method. The research was supplemented with dental and periodontological indicators. The research was supplemented by the application of molecular biology methods related to bacterial DNA isolation, PCR reactions and sequencing. Such selected methods constitute an ideal screening test for the analysis of oral bacterial microbiota. The obtained results suggest that certain types of diet can be an effective prophylaxis in the treatment of civilization diseases such as inflammation of the oral cavity along with periodontal tissues and gingival pockets.

scholarly journals LPS fromP. gingivalisand Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
L. Gölz ◽  
S. Memmert ◽  
B. Rath-Deschner ◽  
A. Jäger ◽  
T. Appel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Periodontal Ligament ◽  
Inflammatory Diseases ◽  
Periodontal Pathogen ◽  
Oral Diseases ◽  
Redox Systems ◽  
Periodontal Ligament Fibroblasts ◽  
Prolonged Incubation ◽  
Periodontal Tissues ◽  
Pdl Cells

Oxidative stress is characterized by an accumulation of reactive oxygen species (ROS) and plays a key role in the progression of inflammatory diseases. We hypothesize that hypoxic and inflammatory events induce oxidative stress in the periodontal ligament (PDL) by activating NOX4. Human primary PDL fibroblasts were stimulated with lipopolysaccharide fromPorphyromonas gingivalis(LPS-PG), a periodontal pathogen bacterium under normoxic and hypoxic conditions. By quantitative PCR, immunoblot, immunostaining, and a specific ROS assay we determined the amount of NOX4, ROS, and several redox systems. Healthy and inflamed periodontal tissues were collected to evaluate NOX4 and redox systems by immunohistochemistry. We found significantly increased NOX4 levels after hypoxic or inflammatory stimulation in PDL cells (P<0.001) which was even more pronounced after combination of the stimuli. This was accompanied by a significant upregulation of ROS and catalase (P<0.001). However, prolonged incubation with both stimuli induced a reduction of catalase indicating a collapse of the protective machinery favoring ROS increase and the progression of inflammatory oral diseases. Analysis of inflamed tissues confirmed our hypothesis. In conclusion, we demonstrated that the interplay of NOX4 and redox systems is crucial for ROS formation which plays a pivotal role during oral diseases.

scholarly journals THE INFLUENCE OF THE AGE ON THE PREVALENCE OF GINGIVAL RECESSION IN PATIENTS WITH PERIODONTITIS

2020 ◽  
pp. 25-31
Author(s):  
P. Mazur ◽  
I. M. Suprunovych
Keyword(s):  
Oral Cavity ◽  
Soft Tissues ◽  
Age Groups ◽  
Gingival Recession ◽  
Root Surface ◽  
Population Exposure ◽  
Moderate Degree ◽  
Gingival Margin ◽  
Periodontal Tissues ◽  
Study Results

Gingival recession is a common clinical condition in the dental practice, which is characterized by the root surface exposure due to the apical displacement of the marginal gingival tissues. Since the teeth' root surfaces become open to the environment of the oral cavity, the consequences of gingival recession are a disturbance of aesthetics (mainly when they occur in the frontal area), the development of hypersensitivity root caries, and non-carious cervical lesions. Despite the numerous studies carried out, the exact mechanism of the development of gingival recession is not fully understood, and it is generally accepted that it has a multifactorial etiology. Gingival recession can occur directly due to an infectious and inflammatory process in the periodontal tissues, anatomical features, as a result of the effect of mechanical or iatrogenic factors on soft tissues, or their action in combination with each other. The prevalence of gingival recessions worldwide varies from 22 to 100% and mainly depends on age, the size of the studied population, diagnostic criteria, and statistical data processing methods.  The aim was to determine the influence of age on the prevalence of gingival recession in patients with periodontitis.  Materials and methods. One hundred thirty-three patients with periodontitis aged 29 to 59 years were included in the study and divided into four groups according to their age: 20–29 years, 30–39 years, 40–49 years, 50–59 years. Inclusion criteria were the presence of periodontitis, age over 20 years, and the presence of more than 20 teeth in the oral cavity.  Participants were interviewed about demographic data, smoking status, and oral hygiene skills. All patients underwent a clinical examination using the clinical and instrumental program Florida Probe system. Gingival recession was recorded in the presence of root surface exposure of 1 mm or more. According to the size of the exposed surfaces of the tooth roots, three degrees of severity of gingival recession were assessed: mild gingival recession (less than 3 mm), moderate gingival recession (3 to 4 mm), severe gingival recession (5 mm or more). The severity of gingival recession was estimated in mm as the distance between the cemento-enamel junction and the gingival margin. Subsequently, the gingival recession was classified using the Miller recession classification [7]. Results of the studies and their discussion. The study results showed that a high prevalence of gingival recession was found in patients with periodontitis in the Ukrainian population. Exposure of the surface of the teeth' roots by 1 mm or more was present in around 2233 teeth, which corresponds to 65.86 (61.4-70.3)% of all examined teeth. The average number of teeth with the gingival recession increases with age: in those aged 20-29, the gingival recession was present in 42,86% of the teeth, in patients 30-39 years of age, the gingival recession was present in around 59,59% of the teeth, in patients 40-49 years of age the gingival recession was present in around 70,49% of the teeth, in those, older than 50 years, the gingival recession was present in around 82.72% of the teeth. Incisors and first molars were the most affected teeth by the gingival recession. In patients with periodontitis, the gingival recession of mild degree (up to 3 mm) was determined around 44.01 (40.8-47.2) % of teeth, the gingival recession of moderate degree (from 3 to 4 mm) – around 17.41 (14.3-20.5) % of teeth, the gingival recession of severe degree (5 mm or more) – around 4.42 (2.7-6.2)% of teeth. The severity of recession in patients of different age groups was determined: the gingival recession's severity increases with age. The average severity of gingival recession (the length from the cemento-enamel junction to the gingival margin) was determined in patients of different age groups: in the group of 20 years old, it was 0.28 ± 0.28 (M ± SD) mm, in the group of 30 years old – 0.43 ± 0.37 (M±SD) mm, in the group of 40 years old – 0.78 ± 0.70 (M ± SD) mm, in the group of 50 years old – 1.20 ± 0.70 (M±SD) mm. According to the Millers classification, Class III of the gingival recession was more common in age groups I and II, Class IV of gingival recession, which have an unfavourable prognosis of treatment, were more often manifested in older patients age groups.

scholarly journals Oral flora of stray dogs and cats in Algeria: Pasteurella and other zoonotic bacteria

2020 ◽  
Vol 13 (12) ◽  
pp. 2806-2814
Author(s):  
Kahina Razali ◽  
Rachid Kaidi ◽  
Amine Abdelli ◽  
Mohamed Nabil Menoueri ◽  
Khatima Ait-Oudhia
Keyword(s):  
Oral Cavity ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Wound Complications ◽  
Bite Wound ◽  
Oral Flora ◽  
Stray Dogs ◽  
Stray Cats ◽  
Zoonotic Bacteria

Background and Aim: Knowledge of potentially pathogenic bacteria presents in the oral cavity of dogs and cats may be helpful in determining appropriate treatment for infected bite wounds. About 120.000 people are exposed to dog and cat bites every year in Algeria, but little is known about the dog and cat oral flora causing bite wound complications. The purpose of this study was to identify potential zoonotic bacteria from oral cavity of dogs and cats and to determine their susceptibility to antibiotics to contribute to the treatment of bite wound infection. Materials and Methods: Oral swabs from 100 stray dogs and 100 stray cats were collected and cultured in several media: Chocolate agar, MacConkey agar, and Mannitol Salt Agar. Bacterial isolates were identified using several commercial kits of the analytical profile index and tested for antibiotic susceptibility by disk diffusion method. Results: Overall, 185/200 (92.5%) dogs and cats carried zoonotic bacteria in their mouths, of which 55.13% (102/185) had at least two bacterial pathogens. 374 pathogenic strains belonging to 15 genera were isolated: Eleven were Gram-negative (Proteus, Pasteurella, Escherichia, Moraxella, Klebsiella, Acinetobacter, Enterobacter, Pseudomonas, Aeromonas, and Neisseria Haemophilus) and four were Gram-positive (Staphylococcus, Streptococcus, and Corynebacterium, Bacillus). Fifty-one strains of Pasteurella were isolated from 44 carriers of Pasteurella (21 Pasteurella multocida, 21 Pasteurella pneumotropica, and 9 Pasteurella spp.). Pasteurella strains were tested for antibiotic resistance. Resistance to at least one drug was observed in 8 (15.68%) of Pasteurella isolates and two strains (3.92%) were found to be multidrug-resistant (to two or more drugs). Erythromycin, penicillin, and ampicillin were the antimicrobials to which the isolates showed greater resistance (7.84%, 5.88%, and 3.92%, respectively). Conclusion: To the best of our knowledge, this study is the first in Algeria to detect potential human pathogenic bacteria in the oral cavity of dogs and cats. It reveals that these animals have multiple zoonotic bacteria in their mouths including Pasteurella species, which may be multidrug-resistant.

The impact of the viral-bacterial consortium on occurrence and development of chronic periodontitis

2020 ◽  
Vol 25 (2) ◽  
pp. 84-89
Author(s):  
V. N. Tsarev ◽  
E. A. Yagodina ◽  
T. V. Tsareva ◽  
E. N. Nikolaeva
Keyword(s):  
Pathogenic Bacteria ◽  
Periodontal Diseases ◽  
Bacterial Consortium ◽  
Progressive Increase ◽  
Bacterial Biofilm ◽  
Herpes Simplex Viruses ◽  
Periodontal Tissues ◽  
Periodontal Inflammation ◽  
Immunological Mechanisms ◽  
The Impact

Relevance. The current theory of specific bacterial biofilm fails explain why a part of patients experiences inflammatory periodontal diseases while the absence of detected specific types of “red complex” bacteria.Purpose. To clarify the microbiological and immunological mechanisms of the influence of the viral and bacterial consortium in the etiology and pathogenesis of inflammatory periodontal diseases.Materials and methods. Articles survey with elements of metanalisis. Literature review based on discussion of research results on the topic of 48 sources including 33 foreign ones.Results. The review provides evidences of the possible participation of viruses of the Herpesviridae family in the development of chronic generalized periodontitis. Evidences for the role of herpes simplex viruses of type 1.2, Epstein-Barr virus, and cytomegalovirus in the development of periodontal inflammation are analyzed. It is proven that all herpesviruses induce the release of proinflammatory cytokines that activate osteoclasts and matrix metalloproteinases, as well as violate antibacterial immune mechanisms. In turn that leads to a progressive increase of periodontal pathogenic bacteria in both the biofilm and periodontal tissues.Conclusion. It is made a conclusion that an active herpetic infection can initiate damage to periodontal tissus and participate in the development of relapses of the disease.

scholarly journals Role of Bacterial Flora in Oral Cancer- An Insight

2021 ◽  
Author(s):  
R Ananthalakshmi ◽  
M Priya ◽  
Nadeem Jeddy ◽  
LJ Sailakshmi
Keyword(s):  
Oral Cavity ◽  
Human Body ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Fungal Species ◽  
The Body ◽  
Oral Carcinoma ◽  
Oral Diseases ◽  
Organ Systems

The human body contains about 1014 bacteria which usually colonise different parts of the body. The bacterial flora is important for a person’s health as well as normal functioning of tissue and organ systems. Bacteria are single celled organisms and are found on almost all surfaces of human body. They act in synergy with host immune mechanism and provide protection against various undesirable foreign invasions, especially in the oral cavity, where they exist in a diversified form which survive in a symbiotic relationship with the host. When there is a disturbance in this equilibrium due to various factors like trauma or tobacco smoking, betel nut chewing and alcohol intake, which makes the mucous membrane more permeable to invading microorganisms, these commensal bacterial species can become virulent and give rise to oral diseases ranging from dental caries to oral carcinoma. The effects of these bacteria can be either direct or indirect initiation of chronic inflammation, formation of procarcinogens that contribute to the development of oral carcinoma. This article focuses on the role of oral bacterial flora in initiation and progression of oral carcinogenesis. Various bacterial and fungal species associated with oral carcinoma and predominant bacterial species in oral cavity and oropharyngeal region along with measures to prevent their adverse effects are described.

scholarly journals The choice of antibacterial drugs in dentistry taking into account the oral microbiome

2021 ◽  
Vol 2 (2) ◽  
pp. 29-35
Author(s):  
I.P. Mazur
Keyword(s):  
Oral Cavity ◽  
Antibacterial Therapy ◽  
Antibacterial Agent ◽  
Oral Microbiome ◽  
Etiological Factor ◽  
Antibacterial Drugs ◽  
Periodontal Tissues ◽  
Inflammatory Processes ◽  
Microbial Associations ◽  
Systemic Antibacterial

The article presents modern data on the features of the oral microbiome and its medical significance when prescribing systemic antibacterial therapy for infectious and inflammatory processes in the oral cavity. The main microbial associations in normal conditions, as well as in diseases of periodontal tissues and apical periodontitis are presented. The use of antibacterial drugs in dentistry is aimed at eliminating the etiological factor of the pathogenic microflora of the oral cavity. The effectiveness of the treatment depends on how well the doctor aware of the main pathogens and microbial associations in generalized periodontitis, periodontitis, a choice of optimal antibacterial agent, compliance with indications and contraindications upon treatment.

scholarly journals Characteristics of dental status of patients with inflammatory periodontal diseases

2017 ◽  
Vol 98 (2) ◽  
pp. 204-210
Author(s):  
N A Vasil’eva ◽  
A I Bulgakova ◽  
E S Soldatova
Keyword(s):  
Oral Cavity ◽  
Soft Tissues ◽  
Periodontal Diseases ◽  
Dental Status ◽  
Control Group ◽  
Periodontal Tissues ◽  
Healthy Donors ◽  
Severe Periodontitis ◽  
Local Trauma ◽  
History Of

Aim. Evaluation of dental status in patients with inflammatory periodontal diseases. Methods. The study of dental status was performed with the use of clinical and historical data from 269 patients with inflammatory periodontal diseases at the age of 18-65 years. Among examined patients there were 75 (27.9%) people with gingivitis, 54 (20.1%) with mild periodontitis, 66 (24.5%) with moderate and 74 (27.5%) with severe periodontitis. Control group consisted of practically healthy donors at the age of 18-52 years (40 people) with sanitized oral cavity. Results. From history and interviewing it was found that 72% of the surveyed patients brush their teeth twice a day, 24% - once a day and 4% of patients do not brush their teeth. Family history of periodontal diseases among parents was recorded by 86% of participants. Dental status was characterized by the increase of the values of all dental indices with increasing disease severity regardless of gender. Need for correction of the depth of the vestibule, lips, cords, and bridles was identified in 51.7% of cases in patients with gingivitis and in 96.6% of cases of periodontal diseases. With increasing severity of periodontitis index (sum) of teeth with decay and fillings and removed teeth was increased by 1.5 times in gingivitis, by 2.2 times in mild periodontitis, by 2.6 times in moderate and by 2.9 times in severe periodontitis compared to control group. Partial adentia in gingivitis is 3 times more prevalent in males than in females and in severe periodontitis is 1.6 times more prevalent in females than in males. Conclusion. Dental status of the patients with inflammatory periodontal diseases was characterized by increased values of all dental indices compared to control group that demonstrates typical course of inflammatory periodontal diseases and decrease of local immunologic resistance of oral cavity and the organism in total; timely correction of local factors (local trauma), anatomical and topographical features of dentofacial system and status of oral soft tissues allows prevention of pathologic processes in periodontal tissues.

scholarly journals Can Plant Materials Be Valuable in the Treatment of Periodontal Diseases? Practical Review

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2185
Author(s):  
Anna Gościniak ◽  
Magdalena Paczkowska-Walendowska ◽  
Agnieszka Skotnicka ◽  
Marek A. Ruchała ◽  
Judyta Cielecka-Piontek
Keyword(s):  
Pathogenic Bacteria ◽  
Antioxidant Activities ◽  
Periodontal Diseases ◽  
Public Awareness ◽  
Ageing Population ◽  
Oral Diseases ◽  
Fruit Peel ◽  
Root Cortex ◽  
Plant Materials ◽  
Periodontal Tissues

Periodontal diseases are one of the most significant challenges in dental health. It is estimated that only a few percent of the worldwide population have entirely healthy teeth, and according to WHO, oral diseases may affect up to 3.5 billion people worldwide. One of the most serious oral diseases is periodontitis, an inflammatory disease affecting periodontal tissues, caused by pathogenic bacteria and environmental factors such as the ageing population, abuse of tobacco products, and lack of adequate oral hygiene due low public awareness. Plant materials are widely and successfully used in the management of many conditions, including periodontitis. Plant materials for periodontitis exhibit antibacterial, anti-inflammatory, antioxidant activities and affect the periodontium structure. Numerous studies demonstrate the advantages of phytotherapy for periodontitis relief and indicate the usefulness of Baikal skullcap root, Pomegranate fruit peel and root cortex, Tea leaves, Chamomile flowers, Magnolia bark, Blackberry leaves and fruits, Cranberry fruits and Lippia sidoides essential oil. This review aims to analyze the use and applicability of selected plant materials in periodontitis management since it is of paramount importance to evaluate the evidence of the traditionally used plant materials in light of continuously growing interest in phytotherapy and its adjuvant role in the treatment of periodontitis.

PHOTODYNAMIC THERAPY WITH METHYLENE BLUE IN CHILDREN WITH CEREBRAL PARALYSIS

2021 ◽  
pp. 31-35
Author(s):  
Kashtanova M.S. ◽  
Morozova N.S. ◽  
Aslanova D.R.
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Bacterial Biofilm ◽  
Treatment Technique ◽  
Healthy Children ◽  
Oral Diseases ◽  
Active Forms Of Oxygen ◽  
Periodontal Tissues ◽  
Cerebral Paralysis ◽  
Dental Diseases

Children with cerebral palsy suffer from dental diseases almost three times more often than healthy children. Risk factors that cause the development of dental diseases in children with CP include low rates of unstimulated salivation rate, acidity, increased saliva osmolarity and total protein concentration, which indicates a violation of hydration. The above factors contribute to the formation of acquired pellicles and biofilms due to increased bacterial agglutination. Ultimately, this contributes to the accelerated formation of plaque. There is a tendency to increase the concentration of hemoglobin and reduce the degree of oxygenation of oxyhemoglobin and, as a result, to increase the active forms of oxygen and nitrogen, which, in turn, have a toxic effect and lead to cell death. Photodynamic therapy (PDT) using photosensitizers (FS) improves blood filling and the degree of oxygenation in periodontal tissues, and also allows the elimination of bacterial biofilm. At the same time, opto-spectral diagnostics painlessly in the monitoring mode allows to identify and fix tissue areas with low hemoglobin oxygenation rates for precision photodynamic effects. PDT is a method used as a therapy for oral diseases of various origins, such as cancer, precancerous diseases, caries, periodontitis and gingivitis. Initially, methylene blue was the first dye used in medicine as an antiseptic, as well as the first tested and approved photosensitizer for antimicrobial photodynamic therapy. The use of photodynamic therapy with methylene blue as a photosensitizer can allow to deactivate inflammation and completely destroy bacteria in the biofilm, which will contribute to the functional normalization of gum tissue and restore oxygenation in the microcirculatory bed. The advantages of this method are antibacterial, anti-inflammatory and wound healing effects. The article presents the literature data on photodynamic therapy with the use of methylene blue in children, on the methodology and results of the use of this treatment technique in practice.

The effect of smoking on periodontal tissues

2020 ◽  
pp. 30-34
Author(s):  
E.D. Kostrigina ◽  
K.I. Тarasenko ◽  
Ya.S. Shinkina ◽  
E.A. Mitryahina ◽  
O.S. Banketova ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Computer System ◽  
Mechanism Of Action ◽  
Diagnostic Method ◽  
Soft Tissues ◽  
Periodontal Diseases ◽  
Treatment Plan ◽  
Etiological Factor ◽  
Periodontal Tissues

This article is a review of the work of domestic and foreign authors on the effect of smoking on periodontal tissues, the mechanism of action of nicotine on the microvasculature, hygiene and soft tissues of the oral cavity. A modern diagnostic method using the Florida Probe computer system is considered. A treatment plan is proposed for patients with inflammatory periodontal diseases, the etiological factor of which is exposure to nicotine.This article is a review of the work of domestic and foreign authors on the effect of smoking on periodontal tissues, the mechanism of action of nicotine on the microvasculature, hygiene and soft tissues of the oral cavity. A modern diagnostic method using the Florida Probe computer system is considered. A treatment plan is proposed for patients with inflammatory periodontal diseases, the etiological factor of which is exposure to nicotine.

Sign in / Sign up

Export Citation Format

Share Document