scholarly journals Surgical Treatment of Displaced Abomasum in Cattle Using Ljubljana Method

2010 ◽  
Vol 79 (3) ◽  
pp. 469-473 ◽  
Author(s):  
Jože Starič ◽  
Halil Selcuk Biricik ◽  
Gurbuz Aksoy ◽  
Tomaž Zadnik

Displacement of the abomasum (DA) is an increasingly common disease in dairy cattle in intensive production. This study presents surgical treatment of DA using Ljubljana method in years 2005 and 2006. Slovenian Black and White dairy cows (n = 30) with left DA in 19 cows, right DA in four cows, anterior DA in two cows and abomasal volvulus in five cows were used. The surgical treatment was successful in 28 cows, 2 had to be euthanized due to complications associated with left DA and abomasal volvulus. One month after the surgery all 28 cows were productive. Omentopexy using Ljubljana method can be used as a clinical procedure as well as procedure performed in ambulatory practice.

2016 ◽  
Vol 52 ◽  
pp. 6-12 ◽  
Author(s):  
M. V. Gladiy ◽  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holyosa ◽  
A. V. Tuchyk ◽  
...  

The main goal of dairy breeds selection should be improving breeding and productive qualities of animals under modern conditions. The majority of farms, using native breeds to produce milk, has created optimal conditions for keeping and feeding, selection and matching, growing of replacements etc. Further improvement of created native dairy breeds for economically useful traits occurs at total use of purebred Holstein bulls (semen) of foreign selection. In order to realistically assess milk productivity (milk yield, fat content in milk and fat yield) of Ukrainian Black-and-White and Red-and-White Dairy cows should be conducted a comparative analysis of Holstein cows under the same conditions of feeding and keeping. It was established that Ukrainian Red-and-White Dairy cows were characterized by the highest milk yields for 305 days of all lactations, taken into account, the among three investigated breeds. Their milk yield during the first lactation was 5933 kg of milk, during the second – 6393 kg, the third – 6391 kg and during higher lactation – 6650 kg. Ukrainian Black-and-White Dairy cows were second by milk yield (except for the second lactation), during the first lactation – 5932 kg of milk, the third – 6462 kg and higher – 6541 kg, and Holstein cows were third, during the first lactation – 5794 kg of milk, the second – 6381 kg, the third – 6335 kg and higher – 6469 kg. The fat content was almost the same and varied within 3.49-3.58% in milk of Ukrainian Red-and-White Dairy cattle, 3.50-3.60% in milk of Ukrainian Black-and-White Dairy cattle and 3.50-3.56% in Holsteins’ milk. The difference between the breeds was within 0.01-0.04%. All the investigated breeds had predominance in fat yield for three lactations over standards of these breeds: Ukrainian Red-and-White Dairy cows from 75.1 to 93.4 kg, Ukrainian Black-and-White Dairy cows – 75.1-89.0 kg respectively and Holstein cows – 41.9-60.2 kg. It was found different level of positive correlation between milk yield and fat yield in all the cases and high correlation (r = 0.604-0.921, P < 0.001) in five cases (41.7%) Negative correlation coefficients indicate that selection of animals to higher milk yield in the herd will decrease the second trait – fat content in milk. Positive and highly significant correlation between milk yield and fat yield indicates that selection of cows in the herd to higher milk yields will increase fat yield. It was revealed that bulls were among the factors impacted the milk productivity (milk yield, fat content, fat yield) of three investigated breeds. So, the force (η²x) of father’s impact on milk yield was15.4-47.9%, fat content – 22.0-43.4% and fat yield – 14.9-47.7% taking into account a lactation and a breed. The force of lines impact (η²x) was second; it was on milk yield 6.1-24.5%, fat content – 4.1-17.1 and fat yield – 5.8-23.5%. The force of breeds impact (η²x) was last; it was on milk yield 0.3-2.9%, fat content – 0.2-0.3% and fat yield – 0.6-2.7%. So, the comparative studies of milk productivity of Ukrainian Red-and-White and Black-and-White Dairy cattle with Holsteins indicate that under similar conditions of feeding and keeping, these native breeds can compete with Holstein cattle. The milk yield for 305 days of higher lactation was 6650 kg of milk in Ukrainian Red-and-White Dairy cows, 6541 kg in Ukrainian Black-and-White Dairy cows and 6469 kg in Holsteins. It was found the inverse correlation r = -0.025-0.316 between milk yield and fat content in milk in most cases. Selection and matching of animals in the herd should be carried out simultaneously on these traits. It was found positive repeatability of milk yields between the first and second, the third and higher lactations (rs = 0.036-0.741), indicating the reliability of forecasting increase in milk productivity during the next lactations in all herd. Bulls have the greatest impact (η²x) on milk productivity among the factors taken into account: milk yield – 15.4-47.9%, fat content in milk – 22.0-43.4% and fat yield – 14.9-47.7%.


2018 ◽  
Vol 51 ◽  
pp. 112-118
Author(s):  
V. V. Machulnyi

Milk yield of cows in breeding herds, Cherkassy region, for last completed lactation averaged 6442 kg of milk with 3.62 % fat content. Currently the question of interconnection of cows’ milk production and reproductive capacity is especially important, because the significant increase in milk yields makes minimize calves. The successful conduct of breeding had set the goal to detect the best options for combining milk production and reproductive ability of animals.To characterize the level of milk production including reproductive ability of cows we used indicator of daily average milk yield per one day of periods between calving (PBC). The best service period can be considered period of 51-90 days, that allows you to get one calf per cow per year (average PBC is 351 days) and maintain high milk production (6431 ± 39.6 kg during 305 days of lactation). With this service period all indicators of milk production and reproductive ability are at high level. Reduced service period below recommended levels will reduce the duration of lactation and milk production, and increasing service period will increase the duration of lactation with a simultaneous reduction in milk production for 305 days. Herewith deterioration of reproductive ability is marked and manifested in the increase of the insemination index, decline in the reproductive capacity, increase of periods between calving and as a result, reduce of calves and culling barren cows.Analysis of genealogical structure of the studied herds points to low diversity (Ukrainian Black-and-White dairy cows belong to 6 lines and Ukrainian Red-and-White dairy cows – to 8 ones) with high affinity (by Chief 1427381.62 to R. Sovereign 198998 bloodline, by Starbuck 352790.79 to Elevation 1491007 and Ideal1013415 bloodlines).Genealogical structure of the herds is saturated a lot of bulls genealogical groups of Holstein cattle: Chief 1427381 – 11 bulls in the array of Ukrainian Black-and-White dairy breed and 5 ones in Ukrainian Red-and-White dairy breed, Starbuck 352790.79 – 10 sires in the array of Ukrainian Black-and-White dairy cattle and 3 ones in Ukrainian Red-and-White dairy breed, Elevation 1491007.65 – 4 sires in the array of Ukrainian Black-and-White dairy cattle and 3ones in Ukrainian Red-and-White dairy breed.Currently the sires of Chief 1427381.62 (7712-3,76-289,9-3,14-242,1), Starbuck 352790 (7857-3,87-295,4-3,07-241,2), Jocko Besne 694028588.94(6687-3,88-259,5-3,03-202,6), and Bell 1667366 bloodlines (61341-3,95-242,3-3,02-185,2) among sires of Ukrainian Black-and-White dairy cattle and the sires of Cavalier 1620273.72 (7285-3,98-289,9-3,08-224,4), Chief 1427381.62 (7522-3,76-282,8-3,00-225,7),Starbuck 392405 (7667-3,87-296,7-3,00-230,0), and Elevation 1491007.65 bloodlines (7544-3,80-286,7-3,5-230,0) among sires of Ukrainian Red-and-White dairy breed are classified to a highly productive and promising for breeding and wider use by productive qualities.After the first insemination the lowest proportion of fertilized cows is among offspring belonging to Chief 1427381 bloodline which is only 38 % among both breeds. However, the milk yield of breeding stock belonging to this bloodline is 7712 kg with 3.76 % fat content for Ukrainian Black-and-White dairy cows and 7522 kg of milk with 3.76 % fat content for Ukrainian Red-and-White dairy cows. Daughters belonging to Elevation1491007, Valiant 1650414, and Hanover 1629391 bloodlines with yields of 4566-5478 kg of milk per lactation had a part of fertilized cows, after the first insemination, at the level of 41-49 %. Fertilization after the first insemination above 50% was in daughters belonging to Cavalier 1620273.72 (52%), Starbuck 392405(50-55 %), Bell 1667366(56%) and Jocko Besne 694028588.94 bloodlines (64 %). The level of performance of these animals for milk yield ranged from 6134 kg to 7857 kg.Heritability coefficients of milk yield and duration of service period were low (0.118-0.289). A positive correlation can be explained by the fact that extension of service period (to a certain level) leads to growing number of milking days (duration of lactation) and defers a decrease in productivity of cows by physiological reasons, namely as a result of pregnancy. Low rates of heritability indicate little genetic diversity of traits and the significant influence of physiological and environmental factors in their formation.As Ukrainian Black-and-White and Ukrainian Red-and-White dairy breeds have a high proportion of heredity of Holstein breed which has genetically large fetus, there is need to study the characteristics of nature passing calving in cows of these breeds. Itis found the number of first-calf heifers which needed help was 52.4%. Animals calve alone in 47.6 % of all cases. Calving was without complications if the live weight of calves not exceeding 6-7% of mother’s weight. The cows calved without help of staff exceeded by 11.7% of the width of the pelvis in the buttocks, 7.5 % of the width of the ilium, and 7.1 % of its oblique length compared with animals of same age.


2016 ◽  
Vol 52 ◽  
pp. 31-36
Author(s):  
V. S. Kozyr ◽  
A. D. Hekkiyev

It was proved that features of lactation curves of cows should be considered at developing breeding programs in dairy cattle breeding, contributing to an objective assessment of a genotype and thus, use of genetic and mathematical methods would increase probability of predicting performance for dairy herd.


2018 ◽  
Vol 5 (1) ◽  
pp. 51-56
Author(s):  
A. Shelyov ◽  
K. Kopylov ◽  
S. Kramarenko ◽  
O. Kramarenko

Aim. The aim of our work was to investigate the interbreed differentiation of Ukrainian Red-and-Motley and Black-and-White dairy cows based on the results of the analysis of allelic and genotypic polymorphism of DNA microsatellites. Methods. Genotyping of 88 DNA samples of two most numerous dairy cattle breeds in Ukraine – Ukrainian Red-and-Motley and Black-and-White dairy cows – was conducted by 10 loci, recommended by the International Society for Animal Genetics (ISAG). The analysis of allelic and genotypic polymorphism was performed using parametric and non-parametric methods. Results. Informative value of DNA microsatellites as markers of genetic processes, which take place in the populations of domestic animals, was assessed. Conclusions. The investigated breeds demonstrate a reliable level of genetic differentiation with a high level of similarity.


2017 ◽  
Vol 54 ◽  
pp. 19-29 ◽  
Author(s):  
N. P. Babik ◽  
Ye. I. Fedorovych ◽  
V. V. Fedorovych

Productive longevity is a very important feature, which is determined by a combination of genotype factors in specific environments. It is known that in recent decades to improve of productive qualities of domestic dairy cattle, the Holstein breeders of different breeds are used. Such measures, although allowed to increase the milk productivity of cows significantly, however, led to reduction in the duration of their productive use. Therefore, the purpose of our research was to study the duration and effectiveness of lifetime use of dairy cows, depending on the country of origin of their father. The research was conducted on animals of Holstein (n = 2902), Ukrainian Black-and-White (n = 14876) and Ukrainian Red-and-White (n = 2176) breeding in various regions of Ukraine. It was established that the controllable number of Holstein cows came from 116 bulls from five different countries. Cows originated from the bulls of Hungarian selection had the longest life and lactation. Cows that came from Canadian bulls had the best life-long yields and life-long number of milk fat. Cows from French bulls were characterized by the worst indicators of productive longevity. Among the descendants of the German bulls, the daughters of the following bulls: Lord 661287, Trend 2761400782690, Bg. Rodeo 27642626161 and Ingo 27677179331, American selection – daughters of B. Astronomer 2160438 and Bob Hubby 2109267, Canadian – daughters of Rock 373840409 and H. R. Artist 6284191, Hungarian – daughters of V.Vilmos 3101733688 and E.Samba 3035115974 and French – daughters of Brico 5794006324 had the best indicators of the duration and efficiency of lifelong use. The studied population of cows of Ukrainian Black-and-White dairy breed came from 167 bulls from eight countries. The largest number of daughters was obtained from domestic bulls (34.6%) and German (29.5%) selection. However, cows of Russian selection had the highest rates of longevity, productive use, lactation, the number of lactations per life, life expectancy and lifetime of milk fat. Daughter Valentina 373840175, Matadora 373840109, Piclenda 373880102 and Tigris 373880127 were lactured more than 4 lactations and had lifelong yield of over 25,000 kg, among the descendants of Russian bulls. Of the descendants of the domestic selection the daughters of the Abrykos 5806 lactated in an average of 6.2 lactation, and their lifetime yields were 38,401 kg. By the number of lactations per life and life-long yields of the daughters of the German bulls, the leader was Presbyter 27621490100, the American – Bob Hubby 2109267 and T. D. Fiasco 1709950, the Canadian – B. Goldgate 6387868 and B. Regensy 394223, the Netherlands – G. Tristan 3021652032 and Baneliai 243931215, Hungarian – E. Samba 3035115974 and P. Selvichar 3023006464. The controlled population of cows of Ukrainian Red-and-White dairy breed came from 69 bulls from five countries. More than 50% of cows of this breed are derived from bulls of German selection. However, the longest duration of productive use and the greatest lifelong yields had daughters of bulls from Canada and Ukrainian selection. Among the descendants of the domestic bulls, according to the indicators of lifetime productivity, the daughters of Khlor 2052 differ markedly. They lacted in average 7.7 lactations, and lifetime longevity was 41,000 kg. Among the descendants of Canadian bulls, the best of their productive use and their lifelong yields had daughters of V. Texel 393522, V.N.Dan 5510544 and Inhibitor 402151, German – Roman 660886883 and Tumpi 112367468, and American – Addikshn 17143107. The influence of the father's country origin on the longevity, productive use, lactation and the number of lactations per life, depending on the breed and the indicator, was within the range of 1.4–17.1, on the indicators of lifetime productivity – within 1.5–9.7%, and the influence of the father on these indicators was, respectively, 9.9–19.9 and 11.4–29.3%.


2018 ◽  
Vol 51 ◽  
pp. 69-73 ◽  
Author(s):  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holysa ◽  
A. V. Tuchyk ◽  
L. V. Marchuk ◽  
...  

Created Ukrainian Red-and-White Dairy and Ukrainian Black-and-White Dairy cattle have a desirable level of reproductive and productive qualities.  These breeds are competing successfully with animals of the European selection, so comparative study of pedigree and productive qualities of animals at one of the farms has practical value. The studies were conducted in the herd of “Oleksandrivske” SE RF at the animals of Ukrainian Red-and-White Dairy and Ukrainian Black-and-White Dairy breeds of Vinnytsia region. The animals of both breeds were kept under the same technological conditions in housing for livestock. It was taken into account milk production (milk yield, fat content in milk and milk fat) for 305 days of lactation. Expert evaluation of the exterior of first-calf heifers has been conducted for 2015-2016. Assessment of type of body structure of cows was conducted on 100-point scale. Fatness of cows was determined on 5-point scale. The analysis of genealogies of 37 bulls which had progeny in the herd was conducted by breeding index, discharge of breeding values and milk production of ancestors. Milk production of the animals of the herd was more than 6000 kg of milk for 305 days of lactation. Comparing milk yields, it was found that the animals of Ukrainian Black-and-White Dairy breed dominated Ukrainian Red-and-White Dairy cows of the same age. It is noteworthy that biological feature of cows to increasing milk yield with each subsequent lactation is positive. Thus, the Ukrainian Black-and-White Dairy cows after the second calving in comparison with the first calving increased milk yield by 380 kg and the animals after the third calving and older comparatively with the second one – by 425 kg more. Also the Ukrainian Red-and-White Dairy cows increased milk yield, respectively, by 348 and 358 kg. The average body measurements of the first-calf heifers were inferior by targeted parameters of Ukrainian Red-and-White Dairy and Ukrainian Black-and-White Dairy cattle from -0.3 cm (girth of metacarpus) to -7.8cm (height in the sacrum) in most cases.  The total number of points for type of body structure was 87.9 at the animals of Ukrainian Black-and-White Dairy cattle and, respectively, 87.0 at the Ukrainian Red-and-White Dairy cows; it corresponds to the assessment "very good". This indicates the high estimation of individual parts of the exterior. The cows of the herd had the required fatness. The average of this trait at animals of Ukrainian Black-and-White Dairy breed was 3.0 points and at the cows of Ukrainian Red-and-White Dairy cattle was respectively 3.4 points. The genealogical structure of the current herd of “Oleksandrivske” SERF consists of two female contingents. The animals of Ukrainian Black-and-White Dairy cattle were derived from 18 bulls belonging to 8 bloodlines. Most of animals belong to R.O.R.Elevation 1491007 bloodline – 45.4%, H.H.Starbuck 352790 and P.F.A.Chief 1427381 bloodlines – 14.4%. Qualitative characteristic of bulls used for the female livestock of the herd, shows that in the vast majority they are valuable breeding bulls. However, it should be noted that there are progeny derived from bulls with low breeding value in the herd. Thus, bull Matrix Red 136549448 had SI-7 and H- and Trubadur 343643346 had SI-50 and H-. The use of bulls for female livestock of the herd with low breeding value is unacceptable for further breeding work. Expectation of improving the breeding traits in progeny from such fathers is unlikely. It is planned to accelerate the reproduction of valuable genotypes and increase the rate of genetic progress at the herd using embryos transplantation this year. Donors of embryos will be their own breeding resources. Using this method will make it possible to exclude from the selection process animals with exterior defects and low-yielding cows. The considered aspects of selective breeding work indicate prospects of using Ukrainian Red-and-White Dairy and Ukrainian Black-and-White Dairy cattle. This applies particularly to increase of milk yield to 8000 kg and more per lactation. Bulls which will be used for breeding stock should have high rates of breeding values (SI +1000 and P4 and more). Especially it applies such features as fat content in milk and some exterior defects.


2000 ◽  
Vol 70 (3) ◽  
pp. 92-101 ◽  
Author(s):  
Burim Ametaj ◽  
Brian Nonnecke ◽  
Ronald Horst ◽  
Donald Beitz

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.


2017 ◽  
Vol 53 ◽  
pp. 115-119
Author(s):  
A. V. Dymchuk ◽  
O. I. Lyubynskyy

The studies of the dynamics results of body weight, absolute and daily average, relative increases averaging bulls of Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Researches are conducted in the conditions of POSP «Napadivske» Kalyinivka district of the Vinnytsya area after materials of primary pedigree account. In an economy use the whole-year of the same type feeding. In the structure of ration 40% make the concentrated sterns and also high-quality silage and soilage. Average daily increases of bulls for period of age to realization for a slaughter made over 1000 grammes. The dynamics of growth of living mass, absolute, average daily and relative increases of bulls, is analysed Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Living mass and weight gain researches of age to 12-monthly age with an interval in 3 months after the generally accepted methods. Materials of researches are worked out the methods of mathematical statistics with the use of programmatic package of Statistica 8.0. Living mass of bulls of the second group at birth made 36,3 kg, that anymore on 0,8 kg by comparison to new-born bulls of the first group. In all next periods living mass of animals of the second group was greater as compared to the animals of the same age of the first. In 3 months advantage made 1,7 kg, in 6 months – 4,6 kg (P>0,95), in 9 months – 8,8 kg (P>0,95), in 12 months – 12,5 kg (P>0,99). Difference of absolute increases of bulls of the second group above the bulls of the same age of the first of age to to 3-monthly age made 0,8 kg in behalf of animals of the second group. In a period 3-6 months a difference is after this index between the first and second groups made 2,9 kg in behalf of the last. In periods 6-9 and reliable advantage of animals of the second group is 9-12 months set above the bulls of the same age of the first on 4,1 kg (P>0,95) and 3,7 kg (P>0,95) accordingly. In a period of age to 18-monthly age absolute increases of bulls of the second group were greater by comparison to bulls first on 12,5 kg (R>0,99). Average daily increases of bulls of the second group of age to 3-monthly age made 763,0 grammes, that anymore on 8,9 grammes by comparison to bulls of the first group. In periods 3-6 months the average daily increases of animals of the second group prevailed the bulls of the same age of the first on 32,6 grammes. In a period 6-9 months the average daily increases of animals of the second group were made by 1045,9 grammes, that for certain anymore on 45,9 grammes (P>0,95) by comparison to the bulls of the same age of the first. Maximal increases of bulls were in a period 9-12 months and made 1210,4 grammes in the animals of the second group and 1168,9 grammes – for the bulls of the same age of the first, that for certain anymore on 41,5 grammes (P>0,95). In a period of age to 12-monthly age the average daily increases of bulls of the second group were made by 970,6 grammes, that was for certain anymore on 31,8 grammes (R>0,99) by comparison to the bulls of the same age of the first group. Relative increases of bulls of both groups were high. From birth to 3-monthly age greater increases had bulls of the first group – 97,8%, and in periods 3-6, 6-9 and 9-12 months greater were increases of bulls of the same age of the second group – 56,4; 40,1 and 32,4% accordingly. For all probed periods of reliable advantage it is not discovered between the compared groups. It is set researches, that at the identical terms of feeding and maintenance more rapid bulls grow Ukrainian red-and-white dairy cattle. In all age-old periods they prevailed the bulls of the same age of the Ukrainian black-and-white dairy cattle, in a that number, beginning from 9-monthly age, advantage was reliable (P>0,95–0,99). Average daily increases of bulls of two breeds were the least in a period of age to to 3-monthly age – 754,1-763,0 grammes, and most in a period 9-12 months – 1168,9–1210,4 grammes.


1953 ◽  
Vol 20 (1) ◽  
pp. 1-12 ◽  
Author(s):  
C. P. Cox

1. Some questions concerning the interpretation of observations on milking dairy cattle are considered.2. A formula is presented for analysing the contributions of changes in the numerator and denominator to the changes in a percentage or fraction, and the formula is applied to changes in the composition of milk.3. The depressions of the butterfat and solids-not-fat percentages consequent on diets reduced in hay in a dairy-cattle feeding experiment are examined with this constituent analysis.The contributions of changes in the magnitudes of the aqueous, butterfat and solids-not-fat constituents to the difference between the butterfat percentages of cows on two treatments, 18 lb. hay/day (control) and 6 lb. hay /day, are about equal. There is an apparent qualitative difference between the effects of the two non-control treatments, and a reduced yield of butterfat would alone largely account for the lower butterfat percentage of cows on the 2 lb. hay /day treatment.


2017 ◽  
Vol 26 (3) ◽  
pp. 292-298 ◽  
Author(s):  
Cesar Augusto Barbosa de Macedo ◽  
Madlaine Frigo Silveira Barbosa de Macedo ◽  
Ana Carolina Miura ◽  
Alessandra Taroda ◽  
Sergio Tosi Cardim ◽  
...  

Abstract The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.


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