scholarly journals The antimicrobial and wound healing effect of Aloe Vera in induced diabetic rats

2014 ◽  
Vol 13 (1) ◽  
pp. 7
Author(s):  
R. A.M. Jawad
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Wound Management ◽  
Control Group ◽  
Leaf Extracts ◽  
Fibroblast Migration ◽  
Aloe Vera Gel ◽  
Group 2

The study was conducted on sixteen streptozotocin induced diabetic rats to investigate whether the topical application of Aloe Vera gel and alcoholic Aloe Vera leaf extract could improve the wound healing in diabetic rats. The rats were weight, matched, and placed in to four groups 4 rats of each group. Full thickness circular skin wound (2cm in diameter) was experimentally created dorsal to the neck area of each rat. Animals in group (1) left without dressing as a control group. Animals in group (2 and 3) were treated topically with Aloe Vera gel (100%) and alcoholic Aloe Vera leaf extracts (100%) respectively, while the animals of group (4) were treated with povidone iodine (4%). Treated groups were dresses twice daily for 14 successive days. The treated wound in groups 2, and 3 were significantly healed faster in comparison to the wound of control group. The effect produced by the Aloe Vera with reference to the wound contraction, wound closure, decreased in surfaces area of wound, and tissue regeneration at the wound site. Histologically wounds treated with Aloe Vera show increase in macrophages, fibroblast migration, collagen regeneration and epithelialization compared with the control group. The wet, dry granulation tissue weight, and hydroxyproline content increased significantly when compared to control. Aloe Vera was seen exhibited antimicrobial activity against some pathogenic microorganism. In conclusion Aloe Vera have strong wound healing property and effective as topical preparation and formulated as fairly economical therapeutic agent for wound management.

scholarly journals Anti-diabetic effect of combined treatment with Aloe vera gel and Metformin on alloxan-induced diabetic rats

2018 ◽  
Vol 4 (1) ◽  
pp. 1-5
Author(s):  
F.O. Atanu ◽  
◽  
O.J. Avwioroko ◽  
S. Momoh ◽  
◽  
...  
Keyword(s):  
Combined Treatment ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Atherogenic Index ◽  
Control Group ◽  
Diabetic Patients ◽  
Aloe Vera Gel ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Aim: The present study investigated the effects of intensive glucose regulation with combined treatment of Aloe vera gel (a herbal agent) and Metformin in an alloxan-induced diabetic rat model. Methods: Forty rats were assigned to the following groups and treated for 21 days: Group 1 (normal untreated control), Group 2 (untreated diabetic rats, DR), Group 3 (DR + 300 mg/kg PE of Aloe vera ), Group 4 (DR + 2 mg/kg Metformin) and Group 5 (DR + 300 mg/kg PE of Aloe vera + 2 mg/kg Metformin). The effect of the treatments on fasting blood glucose (FBG) level, lipid profile, renal function, atherogenic index, pancreas and kidney histopathologies were assessed. Results: FBG level in Group 5 rats decreased by 28.4, 38.0 and 69.0% at Day 7, 14 and 21, respectively. Hyperlipidaemia, high atherogenic index, increased plasma creatinine and urea levels observed in the diabetic rats were ameliorated by the single and combined treatments with Aloe vera and Metformin. Reduced level of HDL-cholesterol in the untreated diabetic rats significantly improved by 160.0, 89.8 and 178.7% respectively in Group 3, 4 and 5 animals. The pancreas and kidney histopathologies indicated signs of recovery in Group 5 rats unlike those of Group 2 that had evidence of necrotic cells in both the acini and islet in pancreas and total glomeruli erosion in kidney. Conclusion: Aloe vera in combination with Metformin for treatment of diabetic patients could avert diabetes-associated dyslipidaemia, improve cellular integrity and increase, thereby preventing patients from risk of cardiovascular diseases and kidney failure.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

scholarly journals A Comparison of Wound Healing Rate Following Treatment with Aftamed and Chlorine Dioxide Gels in Streptozotocin-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Fouad Al-Bayaty ◽  
Mahmood Ameen Abdulla
Keyword(s):  
Wound Healing ◽  
Chlorine Dioxide ◽  
Cellular Proliferation ◽  
Radical Scavenging ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Thickness Skin ◽  
Group 2

Background and Purpose. This study aimed to evaluate the wound healing activities of Aftamed and chlorine dioxide gels in streptozotocin-induced diabetic rats. Experimental Approach. Forty-eight Sprague Dawley rats were chosen for this study, divided into 4 groups. Diabetes was induced. Two-centimeter-diameter full-thickness skin excision wounds were created. Animals were topically treated twice daily. Groups 1, the diabetic control group, were treated with 0.2 mL of sterile distilled water. Group 2 served as a reference standard were treated with 0.2 mL of Intrasite gel. Groups 3 and 4 were treated with 0.2 mL of Aftamed and 0.2 mL of chlorine dioxide gels respectively. Granulation tissue was excised on the 10th day and processed for histological and biochemical analysis. The glutathione peroxidase ,superoxide dismutase activities and the malondialdehyde (MDA) levels were determined. Results. Aftamed-treated wounds exhibited significant increases in hydroxyproline, cellular proliferation, the number of blood vessels, and the level of collagen synthesis. Aftamed induced an increase in the free radical-scavenging enzyme activity and significantly reduced the lipid peroxidation levels in the wounds as measured by the reduction in the MDA level. Conclusions. This study showed that Aftamed gel is able to significantly accelerate the process of wound healing in diabetic rats.

scholarly journals The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Martha Orendu Attah ◽  
Tonye Watson Jacks ◽  
Attah Jacob ◽  
Otong Eduitem ◽  
Barnabas John
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Contraction Rate ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion:  Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

scholarly journals A Comparative Study of the Effects of Nigella sativa Oil Gel and Aloe Vera Gel on Wound Healing in Diabetic Rats

2018 ◽  
Vol 23 ◽  
pp. 2515690X1877280 ◽  
Author(s):  
Yunita Sari ◽  
Iwan Purnawan ◽  
Dhadhang Wahyu Kurniawan ◽  
Eman Sutrisna
Keyword(s):  
Wound Healing ◽  
Nigella Sativa ◽  
Wound Care ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
The Other ◽  
Diabetic Ulcers ◽  
Nigella Sativa Oil ◽  
Aloe Vera Gel ◽  
Important Evidence

Clinicians and wound care nurses in Indonesia usually use Nigella sativa oil (NSO) gel and aloe vera (AV) gel to treat diabetic ulcers. However, there are no studies directly comparing the effects of NSO and AV gels on wound healing, so it is unknown which of these 2 plants is better at promoting wound healing in diabetic ulcers. If the comparative efficacy between these 2 gels was known, it would be important evidence favoring the clinical use of one or the other product in Indonesia. The aim of this study was to investigate and compare the effectiveness of NSO and AV gels on wound healing in a rat model of diabetic ulcers. This experimental study involved 3 groups: NSO gel, AV gel, and controls. Our study showed that from day 5 onward, necrotic tissue and inflammation decreased in the AV gel group compared with the other groups. The wound areas on days 6 ( P = .020) and 7 ( P = .021) were significantly smaller in the AV gel group than in the NSO gel group. Reepithelialization was also better in the AV gel group than in the other groups. This is the first study to compare the effects of AV and NSO gels on wound healing in diabetic ulcers. Our study indicates that the AV gel is better than the NSO gel. Therefore, it is recommended that clinicians and wound care nurses use AV gel instead of NSO gel for the topical treatment of diabetic ulcers.

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

scholarly journals Aloe Gel Enhances Angiogenesis in Healing of Diabetic Wound

2011 ◽  
Vol 3 (3) ◽  
pp. 204
Author(s):  
Djanggan Sargowo ◽  
Adeodatus Yuda Handaya ◽  
Mohammad Aris Widodo ◽  
Diana Lyrawati ◽  
Askandar Tjokroprawiro
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Diabetic Wound ◽  
Aloe Vera Gel ◽  
Diabetic Wound Healing ◽  
Aloe Gel ◽  
Oxide Synthase

BACKGROUND: Diabetic micro and macroangiophathy lead to the incident of diabetic foot ulcers characterized by an increased number of circulating endothelial cells (CECs) and decreased function of endothelial progenitor cells (EPCs). This fact is correlated with ischemia and diabetic wound healing failure. Aloe vera gel is known to be able to stimulate vascular endothelial growth factor (VEGF) expression and activity by enhancing nitric oxide (NO) production as a result of nitric oxide synthase (NOS) enzyme activity. Aloe vera is a potential target to enhancing angiogenesis in wound healing.OBJECTIVE: The objective of this study was to explore the major role of Aloe vera gel in wound healing of diabetic ulcers by increasing the level of EPCs, VEGF, and endothelial nitric oxide synthase (eNOS), as well as by reducing the level of CECs involved in angiogenesis process of diabetic ulcers healing.METHODS: The experimental groups was divided into five subgroups consisting of non diabetic wistar rats, diabetic rats without oral administration of aloe gel, and treatment subgroup (diabetic rats) with 30, 60 and 120 mg/day of aloe gel doses for 14 days. All subgroups were wounded and daily observation was done on the wounds areas. Measurement of the number of EPCs (CD34), and CECs (CD45 and CD146) was done by flowcytometry, followed by measurement of VEGF and eNOS expression on dermal tissue by immunohistochemical method on day 0 and day 14 after treatment. The quantitative data were analyzed by One-Way ANOVA and Linear Regression, with a cofidence interval 5% and significance level (p<0.05) using SPSS 16 software to compare the difference and correlation between wound diameters, number of EPCs and CECs as well as the levels of VEGF and eNOS.RESULTS: The results of this study showed that aloe gel oral treatment in diabetic wistar rats was able to accelerate the wound healing process. It was shown by significant reduction of wound diameter (0.27±0.02); the increased number of CECs (0.42±0.57), respectively (p<0.05). On the other hand, the wound diameter and eNOS indicators showed significant differences at the dose of 60 mg, while the number of EPCs and CECs and the level of VEGF showed significantly different results at a dose of 120 mg. Aloe gel oral therapy showed a positive indication of wound healing acceleration at the optimum dose range 60-120 mg a day.CONCLUSIONS: Aloe gel is potential to be a herbal therapy candidate for diabetic wound healing through enhancing EPCs homing, decreasing the CECs number, and stimulating the increase of VEGF and eNOS levels,hence proving to be a dominant factor in the angiogenesis process.KEYWORDS: aloe gel, diabetes, wound healing, angiogenesis

scholarly journals Evaluating the Effects of Aloe vera Gel on Cerebellum Istomorphometrical Changes in Diabetic Male Rats

2021 ◽  
Vol 23 (3) ◽  
Author(s):  
Mahmood Khaksary Mahabady ◽  
Naeem Erfani Majd ◽  
Mohammad Bahrami Tapebur ◽  
Yazdan Mazaheri
Keyword(s):  
Normal Saline ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Neuroprotective Effects ◽  
Aloe Vera Gel ◽  
Male Wistar Rats

Background: Diabetes mellitus can lead to histomorphometrical changes in the brain. Recent studies have shown that Aloe vera gel has antioxidant and neuroprotective effects, which is independent of glucose-lowering effects. Objectives: The present study aimed to investigate the effects of A. vera gel on histomorphometrical changes of cerebellum following streptozotocin (STZ)-induced diabetic male rats. Methods: A total of 25 male Wistar rats were randomly allocated into five groups as follows: (1) the control group received normal saline; (2) A. vera gel group; (3) diabetic group (normal saline); (4) treatment group diabetic rats, which received A. vera; and (5) diabetic rats which received insulin. A single dose of STZ [60 mg/kg; intraperitoneal (IP)] was used for the induction of diabetes in rats. All the treatments were administered daily for eight weeks. Subsequently, histomorphometrical changes were evaluated in the cerebellum of the rats. Results: The results showed that the number of granular and purkinje cells reduced in the cerebellum granulosa region, while the number of glial cells increased in the molecular region of the cerebellum in diabetic rats compared to the control group (P < 0.05). These changes were improved in treated rats by insulin or A. vera. Also, the thickness of molecular, purkinje, granular, and white matter layers at the apex of lobules and depth of sulcus in the diabetic group had a significant reduction compared to other groups (P < 0.001). Conclusions: Our results confirmed that improvement of the cerebellar tissue changes in diabetic rats following the use of A. vera gel is comparable to insulin. However, more investigations are required to determine the protective effects of A. vera gel against diabetes-induced cerebellum histomorphometrical changes.

scholarly journals A comparative study between aloe vera gel dressing and conventional dressing in chronic wounds

2017 ◽  
Vol 4 (10) ◽  
pp. 3427
Author(s):  
Virendra S. Athavale ◽  
Shivmurti N. Khandalkar ◽  
Megha Mahawar ◽  
Iresh Shetty ◽  
Aditya Lad
Keyword(s):  
Wound Healing ◽  
Surface Area ◽  
Chronic Wounds ◽  
Average Rate ◽  
Aloe Vera ◽  
Cost Effective ◽  
Control Group ◽  
Percentage Reduction ◽  
Aloe Vera Gel ◽  
Group A

Background: The aim of this study was to evaluate the effectiveness and rate of healing of Aloe vera gel in treatment of chronic wounds, to compare the effectiveness of Aloe vera gel with conventional dressing (normal saline and povidone iodine) and to assess the percentage reduction of wound healing with Aloe vera gel dressing.Methods: The study was conducted at Dr. D. Y. Patil Medical College and Hospital, DPU University, for a period of 2 months (from January 2017 to March 2017) and is a prospective and comparative randomized type of study using 50 cases (Group A and Group B, 25 each). The study was approved by the Institute’s Ethics Committee.Results: Data analysis showed that at the end of 4 weeks, mean surface area in Group A is reduced to 4.58 cm2 from 9.79 cm2 which is higher than that in the control group. Also, the average rate of healing in Aloe vera gel is more than control group. Percentage reduction in ulcer surface area was calculated to be much more in Group A as compared to Group B.Conclusions: The study concluded that Aloe vera gel is highly effective in treatment of chronic ulcers and stimulates the growth of wound healing. Thus, reducing the hospital stay. Apart from being efficacious in wound healing, Aloe vera gel is safe product. No allergic reactions/infections were associated with Aloe vera gel. Aloe vera gel not only heals faster but is also cost effective.

scholarly journals Pengaruh Pemberian Gel Lidah Buaya (Aloe vera) Terhadap Jarak Pinggir Luka pada Tikus Wistar

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Farhan Nazir ◽  
Asril Zahari ◽  
Eliza Anas
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Control Group ◽  
Nacl Solution ◽  
Wound Edge ◽  
Control Group Design ◽  
Group Design ◽  
Aloe Vera Gel ◽  
Edge Distance ◽  
Post Test

Abstrak Luka adalah hilang atau rusaknya sebagian jaringan tubuh yang menyebabkan gangguan kontinuitas jaringan tersebut. Lidah buaya telah digunakan sebagai pengobatan tradisional diberbagai kebudayaan diseluruh dunia. Tujuan penelitian ini adalah untuk melihat pengaruh gel lidah buaya terhadap penyembuhan luka, yang ditinjau dari jarakpinggir luka. Penelitian ini merupakan penelitian eksperimental dengan post-test only control group design. Subjek penelitian terdiri dari 24 ekor tikus Wistar betina yang dibagi menjadi empat kelompok; satu kelompok kontrol dan tiga kelompok perlakuan. Kelompok kontrol diberikan NaCl fisiologis sebagai terapi luka sedangkan kelompok perlakuandiberikan gel lidah buaya dengan berbagai frekuensi pemberian. Hasil penelitian menunjukkan bahwa pemberian olesan gel lidah buaya tidak memberikan pengaruh yang signifikan terhadap penyembuhan luka. Pada setiap variabel jarak pinggir luka terlihat bahwa kelompok kontrol memiliki hasil penyembuhan luka yang lebih baik dari hari ke hari. Secara umum, pada tujuh hari pertama (fase hemostasis dan inflamasi) ukuran jarak pinggir luka memiliki perbedaan yang bermakna (p<0,05). Pada hari-hari selanjutnya pada fase proliferasi, ukuran jarak pinggir luka secara umum tidak memiliki perbedaan yang bermakna (p>0,05). Kesimpulan penelitian ini adalah pemberian gel lidah buaya tidak lebih efektif dibandingkan NaCl fisiologis terhadap penyembuhan luka yang ditinjau dari jarak pinggir luka.Kata kunci:  gel lidah buaya, penyembuhan luka, jarak pinggir lukaAbstract A wound is a laceration or break in the living tissue which causes continuity damage within the tissue. Aloe vera has been used for traditional medication in many cultures all over the world. The objective of this study was to see the effect of Aloe vera gel on wound healing based on the wound edge distance. This research was experimental with post-test only control group design. Twenty-four female Wistar rats were divided into four groups; one control group and three treatment groups. The control group was given fisiological NaCl solution for open wound therapy andtreatments groups were given Aloe vera gel with varying frequencies of application. The results shows that Aloe vera wasn’t have a significant effect on wound healing. In each variable of wound edge distance, results show that the control group heals better from day to day. As seen in the general, in the seven first days (hemostatis and nflammation phase), the distance of wound edge was significantly different (p<0.05). In the following days (prol iferation phase), the distance of wound edge generally wasn’t significantly different (p>0.05). The conclusion of this study is Aloe vera gel isn’t effective when compared to fisiologic NaCl solution in healing the wound, based on the wound edge distance.   Keywords:  Aloe vera gel, wound healing, wound edge distance

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