Impact of Biogenic Amines on the Growth of Green Microalgae

2021 ◽  
Vol 11 ◽  
pp. 144-150
Author(s):  
Alexander V. Oleskin ◽  
Andrey L. Postnov ◽  
Cao Boyang

Background: The goal of this research project was to test various neuroactive amines in the capacity of growth stimulators/accelerators of the green microalgae Scenedesmus quadricauda and Chlorella vulgaris that have much biotechnological potential because they can be used for producing drugs, food ingredients, cosmetics, and biofuel. The issue of the ecological role of the biogenic amines in terms of interspecies communication in aqueous ecosystems was also addressed in this work. Methods: S. quadricauda strain GEHD and C. vulgaris strain ALP were cultivated in the light with constant aeration at 24oC in a minerals-containing medium. Experimental systems contained 1, 10 or 100 mM of dopamine hydrochloride, histamine hydrochloride, norepinephrine hydrochloride, or serotonin hydrochloride that were added at inoculation as freshly prepared aqueous solutions. Algal cells were counted using a light microscope , and their number in 1 mL of culture was calculated. The culture liquid and sonicated biomass of S. quadricauda and C. vulgaris were tested for the presence of endogenous amines using high-performance liquid chromatography (HPLC) with an amperometric detector. Results: The biogenic amines serotonin, norepinephrine, dopamine, and histamine significantly stimulated the growth of S. quadricauda, at concentrations of 1 and/or 10 mM but not 100 mM. Histamine was the most efficient stimulator, causing an average 65% increase in biomass accumulation at the end of the cultivation period. The effects of serotonin, dopamine and histamine on C. vulgaris were reported in our previous publication [1], but this work contains the results of our experiments with the previously untested norepinephrine that slightly stimulated the growth of C. vulgaris. HPLC analysis failed to reveal any endogenous amines in the culture liquid and biomass of both microalgae. Conclusions: Since biogenic amines stimulate the growth of the microalgae S. quadricauda and C. vulgaris but are not synthesized by them, we suggest that the algae normally respond to amines produced by other components of aqueous ecosystems, including zooplankton and fish that are known to release significant amounts of biogenic amines into the environment. The data obtained hold some promise with regard to developing a relatively economical technique of boosting algal biomass production.

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Muhammad Asad Ullah ◽  
Faiza Zareen Gul ◽  
Taimoor Khan ◽  
Muhammad Naeem Bajwa ◽  
Samantha Drouet ◽  
...  

AbstractAjuga integrifolia Buch. Ham. ex D.Don, a member of Lamiaceae family is pharmaceutically an active perennial herb widely spread in China, Afghanistan and Pakistan Himalayan region. The application of biotic elicitors is a promising approach to cover limitations of in vitro cell technology and challenges faced by pharmaceuticals industry for bulk up production. The current study involved the induction of agitated micro-shoot cultures with the aim to investigate the growth-promoting as well as phytochemicals enhancement role of yeast extract (YE) and pectin (PE). The results showed that both elicitors induced a considerable physiological response. Biomass accumulation was observed maximum (DW: 18.3 g/L) against PE (10 mg/L) compared to YE and control. Eleven secondary phytocompounds were quantified using high-performance liquid chromatography. PE (50 mg/L) was found to be effective in elicitation of rosmarinic acid (680.20 µg/g), chlorogenic acid (294.12 µg/g), apigenin (579.61 µg/g) and quercetin (596.89 µg/g). However, maximum caffeic acid (359.52 µg/g) and luteolin (546.12 µg/g accumulation was noted in PE (1 mg/L) treatment. Harpagide, aucubin, harpagoside and 8-O-acetyl-harpagoside production was suppressed by both elicitors except for YE (100 mg/L). Catalpol accumulation in micro-shoot cultures was also downregulated except in response to YE (50 and 100 mg/L). Antioxidant activity and anti-inflammatory activity remained higher under PE (50 mg/L) and YE (100 mg/L) respectively. Therefore, results suggested that Ajuga integrifolia micro-shoot cultures treated with yeast extract and pectin might be an efficient bio-factory to produce commercially potent specific secondary metabolites.


Author(s):  
D. E. Newbury ◽  
R. D. Leapman

Trace constituents, which can be very loosely defined as those present at concentration levels below 1 percent, often exert influence on structure, properties, and performance far greater than what might be estimated from their proportion alone. Defining the role of trace constituents in the microstructure, or indeed even determining their location, makes great demands on the available array of microanalytical tools. These demands become increasingly more challenging as the dimensions of the volume element to be probed become smaller. For example, a cubic volume element of silicon with an edge dimension of 1 micrometer contains approximately 5×1010 atoms. High performance secondary ion mass spectrometry (SIMS) can be used to measure trace constituents to levels of hundreds of parts per billion from such a volume element (e. g., detection of at least 100 atoms to give 10% reproducibility with an overall detection efficiency of 1%, considering ionization, transmission, and counting).


2001 ◽  
Vol 17 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Juan Botella ◽  
María José Contreras ◽  
Pei-Chun Shih ◽  
Víctor Rubio

Summary: Deterioration in performance associated with decreased ability to sustain attention may be found in long and tedious task sessions. The necessity for assessing a number of psychological dimensions in a single session often demands “short” tests capable of assessing individual differences in abilities such as vigilance and maintenance of high performance levels. In the present paper two tasks were selected as candidates for playing this role, the Abbreviated Vigilance Task (AVT) by Temple, Warm, Dember, LaGrange and Matthews (1996) and the Continuous Attention Test (CAT) by Tiplady (1992) . However, when applied to a sample of 829 candidates in a job-selection process for air-traffic controllers, neither of them showed discriminative capacity. In a second study, an extended version of the CAT was applied to a similar sample of 667 subjects, but also proved incapable of properly detecting individual differences. In short, at least in a selection context such as that studied here, neither of the tasks appeared appropriate for playing the role of a “short” test for discriminating individual differences in performance deterioration in sustained attention.


2020 ◽  
Vol 16 (5) ◽  
pp. 675-688
Author(s):  
Shamsi Abbasalizadeh ◽  
Behzad Ebrahimi ◽  
Aslan Azizi ◽  
Rogaye Dargahi ◽  
Maryam Tayebali ◽  
...  

Constipation is a common public health concern experienced by all individuals during their life affecting the quality of life. In this paper, we aimed to provide an overview of the existing evidence regarding the role of food ingredients, including bran, prune, fig, kiwifruit, and flax-seed in constipation treatment. We searched Scopus, Pub Med, and Science Direct by using the keywords, “laxative foods” and “constipation”, for searching studies assessing laxative food ingredients and their beneficial effects on constipation treatment and/or control. Lifestyle modifications such as increasing dietary fiber and fluid intake and daily exercise are the proposed first line treatments for constipation. Optimizing ‘diet’ as an efficient lifestyle factor may contribute to the well-being of patients. The use of laxative food ingredients including bran, prune, fig, kiwifruit, flax-seed, probiotics, and prebiotics is a convenient alternative to cope with constipation. According to previous findings, laxative food ingredients could be considered as effective treatments for subjects suffering from constipation. Many studies have assessed the pharmacological and non-pharmacological roles of these ingredients in treating constipation, however, their importance has not been thoroughly investigated.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yasir Islam ◽  
Farhan Mahmood Shah ◽  
Xu Rubing ◽  
Muhammad Razaq ◽  
Miao Yabo ◽  
...  

AbstractIn the current study, we investigated the functional response of Harmonia axyridis adults and larvae foraging on Acyrthosiphon pisum nymphs at temperatures between 15 and 35 °C. Logistic regression and Roger’s random predator models were employed to determine the type and parameters of the functional response. Harmonia axyridis larvae and adults exhibited Type II functional responses to A. pisum, and warming increased both the predation activity and host aphid control mortality. Female and 4th instar H. axyridis consumed the most aphids. For fourth instar larvae and female H. axyridis adults, the successful attack rates were 0.23 ± 0.014 h−1 and 0.25 ± 0.015 h−1; the handling times were 0.13 ± 0.005 h and 0.16 ± 0.004 h; and the estimated maximum predation rates were 181.28 ± 14.54 and 153.85 ± 4.06, respectively. These findings accentuate the high performance of 4th instar and female H. axyridis and the role of temperature in their efficiency. Further, we discussed such temperature-driven shifts in predation and prey mortality concerning prey-predator foraging interactions towards biological control.


2021 ◽  
Vol 23 (3) ◽  
pp. 2038-2045
Author(s):  
Kyoungmin Min ◽  
Young-Han Shin

Prevention of the degradation of sodium-based layered cathode materials is the key to developing high-performance and high-stability sodium-ion batteries.


2021 ◽  
Vol 11 (10) ◽  
pp. 4610
Author(s):  
Simone Berneschi ◽  
Giancarlo C. Righini ◽  
Stefano Pelli

Glasses, in their different forms and compositions, have special properties that are not found in other materials. The combination of transparency and hardness at room temperature, combined with a suitable mechanical strength and excellent chemical durability, makes this material indispensable for many applications in different technological fields (as, for instance, the optical fibres which constitute the physical carrier for high-speed communication networks as well as the transducer for a wide range of high-performance sensors). For its part, ion-exchange from molten salts is a well-established, low-cost technology capable of modifying the chemical-physical properties of glass. The synergy between ion-exchange and glass has always been a happy marriage, from its ancient historical background for the realisation of wonderful artefacts, to the discovery of novel and fascinating solutions for modern technology (e.g., integrated optics). Getting inspiration from some hot topics related to the application context of this technique, the goal of this critical review is to show how ion-exchange in glass, far from being an obsolete process, can still have an important impact in everyday life, both at a merely commercial level as well as at that of frontier research.


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