scholarly journals Induksi Mutasi Fisik dengan Iradiasi Sinar Gamma pada Kacang Merah

2017 ◽  
Vol 1 (1) ◽  
pp. 49
Author(s):  
Siti Marwiyah ◽  
Heny Purnamawati ◽  
Pinni Iyunika Sembiring
Keyword(s):  
Gamma Ray ◽  
Mutation Induction ◽  
Seed Color ◽  
Color Code ◽  
Kidney Beans ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Four Levels ◽  
Red Bean ◽  
Dose Change

Physical mutation induction by gamma ray irradiation can increase the genetik diversity to support the selection ofcertain characters in plant breeding. Varieties is the key to improving the productivity of kidney beans (Phaseolus vulgaris) in Indonesia. This study aimed to study the effect of physical mutation induction by gamma ray irradiation of the red bean genotypes Brast-1. The research was conducted from February to May 2016. The genetik material Brast genotype-1 was irradiated with four levels of gamma ray (55, 110, 165 and 220 Gy). Weight of 100 grains M1 at a dose of 55 Gy were significantly different from M0 is a positive change from the gamma ray irradiation treatment. Effect of gamma ray irradiation was indicated by the changing of the morphology of leaves, pods and seed color code of M2.Keywords: dose, change, genotype, M2 seed

scholarly journals Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation

2015 ◽  
Vol 2 (1) ◽  
pp. 26-32
Author(s):  
Syarifah Iis Aisyah ◽  
Yodi Marthin ◽  
M. Rizal M. Damanik
Keyword(s):  
Gamma Irradiation ◽  
Dose Level ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
Coleus Blumei ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Short Time

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun

scholarly journals Gamma Irradiation Treatment of Bactrocera dorsalis Hendel (Diptera: Tephritidae) in Snake Fruit

2019 ◽  
Vol 23 (2) ◽  
pp. 242
Author(s):  
Novi Kusumaning Astuti ◽  
Suputa Suputa ◽  
Nugroho Susetyo Putra ◽  
Murni Indarwatmi
Keyword(s):  
Gamma Ray ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
The Third ◽  
Minimum Dose ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

Snake fruit (Salacca zalacca) is a unique fruit and it has decadent prospects to be developed as an export commodity. Nevertheless, oriental fruit fly, Bactrocera dorsalis Hendel seems to decrease the quantity and quality of this fruit. On the other side, irradiation has been developed as a standard quarantine treatment to disinfest fruit fly on fruit. The objective of this research was to determine impact of irradiation by Cobalt-60 gamma-ray on the development and survivorship of eggs and the third instar of fruitfly larvae using in-vitro and in-vivo approaches and minimum dose of Cobalt-60 gamma rays applied for snake fruit. Six doses of gamma-ray, i.e. 0 (control), 25, 50, 75, 100, 125 and 150 Gy were used in this experiment. The results showed that Cobalt-60 disturbed development and survivor rate of B. dorsalis. The development of eggs into pupa was failed when treated with Cobalt-60 at any doses, while the third instar larvae failed to become adult when irradiated with 75, 100 and 150 Gy of gamma-ray. The impacts were increased with the increment of dose. Furthermore, impact of gamma-ray irradiation was greater on eggs compared to the third instar of larvae. The minimum dose of irradiation to prevent adults emerge was 118 Gy.

scholarly journals Keragaman warna bunga m1 tanaman aster (Callistephus chinensis) Hasil induksi mutasi iradiasi sinar gamma

2018 ◽  
Vol 2 (1) ◽  
pp. 52
Author(s):  
Yanuar Rizqiani ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Research Design ◽  
Gamma Ray ◽  
Flower Colour ◽  
Purple Colour ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Flower Diameter ◽  
Gamma Ray Irradiation ◽  
Completely Randomized Design ◽  
Stalk Length

The aims of research was to determine the effect of gamma ray on the flower colour of Daisies. The research design was completely randomized design with five replicates. The irradiation treatment of gamma ray were 0 Gy, 5 Gy, 10 Gy, 15 Gy, 20 Gy. Parameters observed were stalk length, time of flowering, number of flowers, flower diameter, and flower colour. The collected data were analyzed by analysis of variance (ANOVA) and continued by Least Significance Different (LSD) of 5% level. The result showed that irradiation of gamma ray did not affected stalk length, number of flowers, and time of flowering. The gamma ray irradiation had a significant effect on flower diameter. Gamma ray irradiation significantly decreased the diameter of flower. The flower colour at doses 0 Gy (control) was purple. Colour flower was varied from dark purple to pink at irradiation 10 Gy and 15 Gy. Keywords: Callistephus chinensis, mutation, irradiation, colour of flower.

scholarly journals Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

2008 ◽  
Vol 58 (3) ◽  
pp. 331-335 ◽  
Author(s):  
Hiroyasu Yamaguchi ◽  
Akemi Shimizu ◽  
Konosuke Degi ◽  
Toshikazu Morishita
Keyword(s):  
Dose Rate ◽  
Dna Content ◽  
Gamma Ray ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Mutation Induction ◽  
Gamma Ray Irradiation

scholarly journals Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Rossa Yunita ◽  
ISWARI S. DEWI ◽  
ENDANG GATI LESTARI ◽  
RAGAPADMI PURNAMANENGSIH ◽  
SUCI RAHAYU ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Gamma Ray ◽  
Upland Rice ◽  
Mutation Induction ◽  
Selection Methods ◽  
Rice Varieties ◽  
Drought Tolerant ◽  
Gamma Ray Irradiation

Abstract. Yunita R, Dewi IS, Lestari EG, Purnamanengsih R, Rahayu S, Mastur. 2020. Formation of upland rice drought-tolerant mutants by mutation induction and in vitro selection. Biodiversitas 21: 1476-1482. Increased production can be done by increasing the planting area, but the available land is sub-optimal land with drought stress. Drought-tolerant rice varieties are needed to utilize this land. To create drought-tolerant rice can use induction mutation and in vitro selection methods. This study aims to obtain tolerant upland rice mutants through mutation and in vitro selection. The parents used were Batutegi and Situpatenggang varieties. This research consisted of several main activities, namely callus induction, determination of LD 50% value ofPEG determination of LC 50% value of gamma-ray irradiation, mutation induction and in vitro selection, bud regeneration and acclimatization. The results of this study are mutant callus formed by gamma-ray irradiation at a dose of 24.68 Gy for Situpatenggang and 22.15 Gy for Batutegi (LD50) and selected on PEG media at a dose of 24.11% for Situpatenggang and 25.18% for Batutegi (LC50). The mutant callus regenerated on MS + BA 3 mg/L + Zeatin 0.1 mg/L produced 83 Situpatenggang shoots and 73 Batutegi shoots and successfully acclimatized were 52 Situpatenggang lines and 49 Batuteg linesBatutegi

scholarly journals Induksi Mutasi Kara Benguk (Mucuna pruriens L.) Menggunakan Iradiasi Sinar Gamma

2020 ◽  
Vol 22 (2) ◽  
pp. 105
Author(s):  
Siti Hartati Yusida Saragih ◽  
Khairul Rizal ◽  
Kamsia Dorliana Sitanggang
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mucuna Pruriens ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Group Design ◽  
Planting Material ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves

<p>Breeding kara benguk with mutation induction techniques using gamma ray radiation is expected to produce genetic diversity. The purpose of the study is to determine the value of Lethal Dose 50 (LD50) and obtain the genetic diversity of the kara benguk plant. The research was conducted at PAIR BATAN and Agrotechnology experiment, Universitas Labuhanbatu from May-July 2020. The planting material used was kara benguk seeds. The method used was Randomized Complete Group Design (RCGD) with one factor which was dose of irradiation with six levels (0, 200, 250, 300, 350 and 400 Gy) and three replications. Each replication consisted of 20 plants, so there are a total of 360 experimental plants. The results showed that the value of LD50 kara benguk in 3 weeks after planting was 281,472 Gy. Gamma ray irradiation increases the high genetic diversity of plants and the number of leaves at a dose of 350 Gy.</p>

scholarly journals Keragaan Anggrek Persilangan ♀ Vanda Celebica X ♂ Vanda Dearei Hasil Iradiasi Sinar Gamma

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Sri Hartati ◽  
Ahmad Yunus ◽  
Fajar Nugroho
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Color Change ◽  
Leaf Length ◽  
Leaf Color ◽  
Color Changes ◽  
Dose Irradiation ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

<p>Variation on orchid is an advantage that allows plant as breeding material. Orchid in plant breeding has a target to increase plant genetic diversity character who favored by the consumer. Efforts to increase genetic diversity among others by artificial mutation using gamma ray irradiation. This research aims to study the influence of gamma ray irradiation dose against the performance of morphology and growth of crossbred orchids ♀Vanda celebica x ♂Vanda dearei. Research using completely randomized design single factor that is dose gamma ray irradiation 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray and 50 Gray. Data were analyzed descriptively, compared plant irradiation treatments with control plants to determine the effect of irradiation on morphology and growth plant. The results showed dose irradiation treatment increased the diversity of plant height, leaf amount, leaf length, leaf color changes, the amount and length of roots. 10 Gray dose irradiation increases as height and length plant leave. 30 Gray dose irradiation increase in the number of leaves. Leaf color change occurs at 20 irradiation treatments Gray and 50 Gray.</p>

scholarly journals Radiosensitivitas Kalus Embriogenik Kopi Robusta Bp 436 Terhadap Iradiasi Sinar Gamma

2019 ◽  
Vol 6 (1) ◽  
pp. 41
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
Enny Randriani ◽  
Laela Sari ◽  
Anne Nuraini
Keyword(s):  
Genetic Diversity ◽  
Somatic Embryos ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Robusta Coffee ◽  
Randomized Design ◽  
Young Leaves ◽  
Gamma Ray Irradiation

<p><em>High genetic diversity is one factor that determines the success of plant breeding. Mutation induction by gamma ray irradiation is one method to improve plant genetic diversity. This study aimed to 1) obtain growth regulators composition suitable in inducing embryogenic callus, 2) determine the effect of gamma ray irradiation on the growth and development of somatic embryos, and 3) obtain lethal dose (LD) <sub>20</sub> and <sub>50</sub> values in Robusta coffee  BP 436. The study was conducted at the Tissue Culture Laboratory, Industrial and Beverage Crops Research Institute,  from May 2017 to December 2018. Explants used were young leaves of Robusta coffee BP 436. Callus induction used ½ MS media with 2,4-D (4.52 μM) and 2-iP (0.00; 4.93; 9.86; 14.79; and 19.72 μM) treatment. Mutation induction was performed using gamma radiation dosed at 0, 10, 20, 30, 40, and 50 Gy treatments. The regeneration media was ½ MS containing GA<sub>3</sub> (0 and 1 mg/l). The study used a complete randomized design with 10 replications. The results showed the best combination of plant growth regulator to induce the callus was 2.4-D 4.52 μM + 2-iP 19.72 μM. The fresh weight of cultures was inhibited above 30 Gy, whereas the number of somatic embryos decreased at doses above 10 Gy. Addition of GA<sub>3</sub> 1 mg/l in regeneration media increased the number of somatic embryos in torpedo phase, but not in gamma irradiation exposed calluses. The LD<sub>20</sub> and LD<sub>50</sub> of Robusta coffee BP 436 are 16.81 and 28.52 Gy, respectively.</em></p>

scholarly journals Studies on the Preservation of Foods and the Changes of their Nutrients Components by Gamma-Ray Irradiation IV. Effects on the red bean

RADIOISOTOPES ◽  
1965 ◽  
Vol 14 (2) ◽  
pp. 93-96
Author(s):  
Seiko OKANOUE ◽  
Matue FUKUTANI ◽  
Mizuho SUGIHARA ◽  
Isao HASHIDA
Keyword(s):  
Gamma Ray ◽  
Gamma Ray Irradiation ◽  
Red Bean
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