scholarly journals Keragaman warna bunga m1 tanaman aster (Callistephus chinensis) Hasil induksi mutasi iradiasi sinar gamma

2018 ◽  
Vol 2 (1) ◽  
pp. 52
Author(s):  
Yanuar Rizqiani ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Research Design ◽  
Gamma Ray ◽  
Flower Colour ◽  
Purple Colour ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Flower Diameter ◽  
Gamma Ray Irradiation ◽  
Completely Randomized Design ◽  
Stalk Length

The aims of research was to determine the effect of gamma ray on the flower colour of Daisies. The research design was completely randomized design with five replicates. The irradiation treatment of gamma ray were 0 Gy, 5 Gy, 10 Gy, 15 Gy, 20 Gy. Parameters observed were stalk length, time of flowering, number of flowers, flower diameter, and flower colour. The collected data were analyzed by analysis of variance (ANOVA) and continued by Least Significance Different (LSD) of 5% level. The result showed that irradiation of gamma ray did not affected stalk length, number of flowers, and time of flowering. The gamma ray irradiation had a significant effect on flower diameter. Gamma ray irradiation significantly decreased the diameter of flower. The flower colour at doses 0 Gy (control) was purple. Colour flower was varied from dark purple to pink at irradiation 10 Gy and 15 Gy. Keywords: Callistephus chinensis, mutation, irradiation, colour of flower.

scholarly journals Keragaan Anggrek Persilangan ♀ Vanda Celebica X ♂ Vanda Dearei Hasil Iradiasi Sinar Gamma

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Sri Hartati ◽  
Ahmad Yunus ◽  
Fajar Nugroho
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Color Change ◽  
Leaf Length ◽  
Leaf Color ◽  
Color Changes ◽  
Dose Irradiation ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

<p>Variation on orchid is an advantage that allows plant as breeding material. Orchid in plant breeding has a target to increase plant genetic diversity character who favored by the consumer. Efforts to increase genetic diversity among others by artificial mutation using gamma ray irradiation. This research aims to study the influence of gamma ray irradiation dose against the performance of morphology and growth of crossbred orchids ♀Vanda celebica x ♂Vanda dearei. Research using completely randomized design single factor that is dose gamma ray irradiation 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray and 50 Gray. Data were analyzed descriptively, compared plant irradiation treatments with control plants to determine the effect of irradiation on morphology and growth plant. The results showed dose irradiation treatment increased the diversity of plant height, leaf amount, leaf length, leaf color changes, the amount and length of roots. 10 Gray dose irradiation increases as height and length plant leave. 30 Gray dose irradiation increase in the number of leaves. Leaf color change occurs at 20 irradiation treatments Gray and 50 Gray.</p>

scholarly journals Microplastic Depuration on Asaphis Detlorata

2020 ◽  
Vol 1 (2) ◽  
pp. 37-46
Author(s):  
Dian Fatriani Indah Saputri ◽  
Anwar Daud ◽  
Rachman Syah ◽  
Agus Bintara Birawida ◽  
Hasnawati Amqam ◽  
...  
Keyword(s):  
Research Design ◽  
Analysis Of Variance ◽  
Experimental Animal ◽  
Experimental Research ◽  
River Estuary ◽  
Circulation System ◽  
Randomized Design ◽  
Water Circulation System ◽  
Completely Randomized Design ◽  
Post Hoc

Depuration is an effort to reduce/eliminate contamination including microplastics, which one is using a water circulation system. This study aims to determine the effective depuration time to reduce the microplastic content in Asaphis detlorata This study used a quantitative approach with experimental research design with a completely randomized design. There are 450 shells used as an experimental animal where is the treatment consisted of four depuration times, namely 1;2;3; and 4 days with 3 repetitions of each treatment, while the control shells were without depuration. Analysis of variance (ANOVA) was used to see the effect of depuration treatment on the microplastic content. If the effect of the treatment was significantly different, then the post hoc test was continued to determine the differences between treatments. The results showed that Asaphis detlorata obtained from the mouth of the Lakatong river estuary were contaminated with microplastics ranging from 0.6 to 8.1 MPs/shellfish and an average of 3.96 MPs/shellfish. Depuration time significantly affected the microplastic content in shellfish depuration effectiveness. There is a tendency that the longer depuration time is decreased microplastic content in shellfish. The effective depuration time to reduce the microplastic content in Asaphis detlorata was 3 and 4 days. Further research is needed for a more effective depuration for cleaning microplastics in shellfish.

scholarly journals Induksi Mutasi Kalus Embriogenik Gandum (Triticum aestivum L.) melalui Iradiasi Sinar Gamma untuk Toleransi Suhu Tinggi

2015 ◽  
Vol 43 (1) ◽  
pp. 36 ◽  
Author(s):  
Ryan Budi Setiawan ◽  
Nurul Khumaida ◽  
Diny Dinarti
Keyword(s):  
High Temperature ◽  
Embryogenic Callus ◽  
Genetic Improvement ◽  
In Vitro Selection ◽  
Gamma Ray ◽  
Triticum Aestivum L ◽  
Temperature Tolerance ◽  
Randomized Design ◽  
Gamma Ray Irradiation

Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20±4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.<br />Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo

scholarly journals Total of Escherichia coli Excreta Broiler Given Enterococcus sp. as Probiotics Candidate of Poultry

2019 ◽  
Vol 4 (1) ◽  
pp. 6-12
Author(s):  
Muhammad Nur Hidayat ◽  
Khaerani Kiramang ◽  
Felis Gunawan
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Digestive Tract ◽  
Research Design ◽  
Antimicrobial Compound ◽  
Randomized Design ◽  
Completely Randomized Design

The purpose of this study was to see the effect of giving Enterococcus sp. bacteria at Escherichia coli of excreta broiler. The research design used was a completely randomized design consisting of four treatments, three replications, and each replication had four broilers. The treatment given consisted of T1 (0 mL/L), T2 (1 mL/L), T3 (3 mL/L), and T4 (5 mL/L) control treatments. The number of colonies of Enterococcus sp. given through drinking water every day, namely 107 CFU / mL. The results of the study respectively showed the number of Escherichia coli in the treatment of T1 (Log 7.54 CFU/g), T2 (Log 7.53 CFU/g), T3 (Log 7.48 CFU/g), and T4 (Log 6.78 CFU/g). The colony total of Escherichia coli of broiler excreta decreases with increasing doses of Enterococcus sp. It is shown that Enterococcus sp. has an antimicrobial compound activity which can inhibit pathogen growth in broiler digestive tract so that it has the potential to be developed as a probiotic

scholarly journals KUALITAS MOTILITAS DAN VIABILITAS SPERMATOZOA DARI SEMEN AFKIR SAPI LIMOUSIN PADA PENGENCER SUSU SKIM KUNING TELUR SITRAT DENGAN PENAMBAHAN BERBAGAI KADAR GLUKOSA

2020 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Rudi Irvanto ◽  
Hardijanto Hardijanto ◽  
Widya Paramita ◽  
Suherni Susilowati ◽  
Tita Damayanti L ◽  
...  
Keyword(s):  
Research Design ◽  
Glucose Level ◽  
Skim Milk ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Design ◽  
Completely Randomized Design

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Semen observation was done at 0 hour, 24 hours and 48 hours. Research design used in this study was completely randomized design with faktorial pattern with 5 replicates. Highest result in motility this research was showed at 24 hours with 30% value in glucose 2,5% treatment and 48 hours with 10% value. The lowest result showed in glucose 0% treatment at 24 hours and 0% at 48 hours. Highest result in viability showed on glucose 2,5% treatment with 62,6% value at 24 hours and at 48 hours with 53,4% value. Lowest result in viability showed on glucose 0% treatment with 44,2% value at 24 hours and 31,4% value at 48 hours.

scholarly journals SOMACLONAL PUTATIVE MUTANTS OF RICE TOLERANT TO SALINITY

2018 ◽  
Vol 19 (2) ◽  
pp. 67
Author(s):  
Rossa Yunita ◽  
Nurul Khumaida ◽  
Didy Sopandie ◽  
Ika Mariska
Keyword(s):  
Salinity Stress ◽  
Gamma Ray ◽  
Mutation Induction ◽  
Width Ratio ◽  
Growth Media ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design ◽  
Mg Content

<p class="abstrakinggris">Soil salinity could significantly reduce rice yield, therefore, varieties tolerant to salinity are urgent to be developed. Mutation induction could be used to create rice mutants tolerant to salinity. The study aimed to evaluate the tolerance of somaclonal mutants of rice to NaCl salinity in a greenhouse condition and characterize their tolerance mechanism. A total of 45 putative mutants were generated by a gamma ray mutation induction followed with <em>in vitro</em> selection in the growth media containing different NaCl concentrations in the greenhouse experiment. The study consisted of two-factor treatments, namely three levels of NaCl concentrations and 45 rice mutants suspected to be tolerant to salinity, arranged in a completely randomized design. Proline, cations (K, Na, Ca, and Mg) content, and stomata density were evaluated.  The results showed that eight mutants were tolerant to 150 mM NaCl, namely CH30, CH-4-2, II-13-42, II-13-7, II-13-10, II-13-13, II-13- 2, and IA-3-21. These tolerant mutants had a higher Na content compared to the check parent. The tolerant mutants had a high proline content, lower Na, and stable K, Mg and Ca cations as well as had a greater number of stomata and higher stomata length-width ratio. Some of the identified tolerant mutants demonstrated the tolerant mechanism against salinity stress. Further studies are required to evaluate these tolerant mutants in the field conditions under salinity stress.</p>

scholarly journals Gamma Irradiation Treatment of Bactrocera dorsalis Hendel (Diptera: Tephritidae) in Snake Fruit

2019 ◽  
Vol 23 (2) ◽  
pp. 242
Author(s):  
Novi Kusumaning Astuti ◽  
Suputa Suputa ◽  
Nugroho Susetyo Putra ◽  
Murni Indarwatmi
Keyword(s):  
Gamma Ray ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
The Third ◽  
Minimum Dose ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

Snake fruit (Salacca zalacca) is a unique fruit and it has decadent prospects to be developed as an export commodity. Nevertheless, oriental fruit fly, Bactrocera dorsalis Hendel seems to decrease the quantity and quality of this fruit. On the other side, irradiation has been developed as a standard quarantine treatment to disinfest fruit fly on fruit. The objective of this research was to determine impact of irradiation by Cobalt-60 gamma-ray on the development and survivorship of eggs and the third instar of fruitfly larvae using in-vitro and in-vivo approaches and minimum dose of Cobalt-60 gamma rays applied for snake fruit. Six doses of gamma-ray, i.e. 0 (control), 25, 50, 75, 100, 125 and 150 Gy were used in this experiment. The results showed that Cobalt-60 disturbed development and survivor rate of B. dorsalis. The development of eggs into pupa was failed when treated with Cobalt-60 at any doses, while the third instar larvae failed to become adult when irradiated with 75, 100 and 150 Gy of gamma-ray. The impacts were increased with the increment of dose. Furthermore, impact of gamma-ray irradiation was greater on eggs compared to the third instar of larvae. The minimum dose of irradiation to prevent adults emerge was 118 Gy.

scholarly journals Evaluasi Persentase Karkas Ayam Kampung Super Dengan Pemberian Jermai Jagung Fermentasi

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Frangki Sigaha ◽  
Ellen J Saleh ◽  
Srisukmawati Zainudin
Keyword(s):  
Research Design ◽  
Analysis Of Variance ◽  
Carcass Weight ◽  
Corn Straw ◽  
Average Value ◽  
Randomized Design ◽  
Native Chicken ◽  
Completely Randomized Design ◽  
Carcass Cuts

This study aims to determine the evaluation of the percentage of super native chicken carcasses by providing fermented corn straw. The research design used in the study was a Completely Randomized Design consisting of 5 treatments and 4 replications namely P0 (ration without treatment), P1 (ration containing 5% fermented corn straw), P2 (ration containing 10% fermented corn straw), P3 (ration ration containing 15% fermented corn straw) and P4 (ration containing 20% fermented corn straw). The data obtained were then analyzed using the Analysis of variance (ANOVA) and if the results were influential then proceed with the Duncan Distance test. The results showed that the treatment had no significant effect (P> 0.05) on the variables of life weight, carcass weight and percentage of carcass cut. The highest living weight was found in the P0 treatment (without using fermented corn straw) with the acquisition of an average value of 850.75 gr / head and produced the highest carcass weight of 197.25 gr. The highest percentage of carcass cuts was obtained at P3 treatment (15% fermented corn straw) with an average value of 23.88%.

scholarly journals Karakter Fisiologi dan Pendugaan Heritabilitas Tanaman M1 Bawang Merah (Allium Ascalonicum L.) Hasil Induksi Iradiasi Sinar Gamma

2018 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Lika Alfariatna ◽  
Florentina Kusmiyati ◽  
Syaiful Anwar
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Gamma Ray ◽  
Reductase Activity ◽  
Randomized Design ◽  
Heritability Estimation ◽  
Value Range ◽  
Gamma Ray Irradiation ◽  
Lethal Doses ◽  
Physiological Characters

ABSTRACTThe aim of the research was to determine the physiological characters, heritability estimation, and information of lethal doses (LD50) of M1 onion plant induced by gamma ray irradiation. The research was arranged in monofactor experimental with Complete Randomized Design (CRD) with 6 treatments and 5 replication, each replication consisted of 5 onion bulbs was irradiated by gamma ray of 0, 3, 6, 9, 12, 15 Gy. Parameters observed: the percentage of germination, chlorophyl content, nitrate reductase activity, heritability estimation and LD50. The data were analyzed by anova and followed by BNT 5% level. The result showed that irradiation significant affected to chlorophyl content and nitrate reductase activity. LD50 of onion is 7,64 Gy. Heritability value range from 52,22 – 80,51%. Keywords : Allium ascalonicum L.,irradiation, LD50, heritability

Sign in / Sign up

Export Citation Format

Share Document