scholarly journals An Economical Non-Antibiotic Alternative to Antibiotic Therapy for Subclinical Mastitis in Cows

2021 ◽  
Vol 41 (04) ◽  
pp. 475-480
Author(s):  
Misbah Ijaz
Keyword(s):  
Antibiotic Therapy ◽  
Trisodium Citrate ◽  
Subclinical Mastitis ◽  
Comparable Efficacy ◽  
Buffer System ◽  
Suitable Alternative ◽  
Cell Counts ◽  
Oral Formulations ◽  
Cu And Zn ◽  
Vit C

The economic importance of mastitis and antibiotic resistance is dictating to search non-antibiotic alternatives for the therapy. Trisodium citrate (TSC) being buffer system of the glandular tissue and, vitamin C (Vit. C), zinc and copper being important ingredients required for functioning of immune system fancy chances for a suitable alternative mastitis therapy. The current study was planned to evaluate therapeutic efficacy and cost effectiveness of these ingredients in subclinical mastitis. For this purpose, 40 sub-clinically mastitis cows were randomly divided into 2 equal groups. Group T1 was treated orally with TSC, Vit. C, ZnSo4 and CuSo4, while group T2 was treated with standard antibiotic therapy. Milk pH significantly (P<0.05) differed between the two treatments till day 7th post-initiation of treatment when T1 restored the pH values within normal range earlier than T2. A non-significant (P>0.05) difference was observed in milk pH, fat, lactose, proteins, TS, SNFs, somatic cell counts and restoration of milk yield between the two treatments indicting comparable efficacy. A statistically significant (P<0.05) difference was observed in serum Cu and Zn levels indicating that the supplementation of Cu and Zn led to higher serum values in animals of T1. The use of non-antibiotic oral formulations as mastitis therapy resulted in a net profit of Rs. 457/animal/day. The oral non-antibiotic antibacterial formulation is a therapeutically and economically suitable alternative to rational antibiotic-based therapy to treat subclinical mastitis in dairy cows.

scholarly journals Will Fly Repellency Using Deltamethrin Reduce Intramammary Infections, Stress and Fatigue Indicators of Dairy Ewes under Intensive Management?

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 232
Author(s):  
Konstantinos V. Arsenopoulos ◽  
Georgios Sioutas ◽  
Eleutherios Triantafillou ◽  
Athanasios I. Gelasakis ◽  
Elias Papadopoulos
Keyword(s):  
Creatine Kinase ◽  
Animal Health ◽  
Serum Cortisol ◽  
Antibiotic Use ◽  
Subclinical Mastitis ◽  
Northern Greece ◽  
Intramammary Infections ◽  
Dairy Ewes ◽  
Stressful Environment ◽  
Cell Counts

Intramammary infections (IMIs) caused by various pathogens may lead to clinical or subclinical mastitis, challenging the health and welfare status of infected animals and decreasing the quantity and quality of the produced milk. Additionally, the zoonotic potential of some of the pathogens isolated from IMI cases, the emergence of antibiotic resistance due to the extensive antibiotic use for IMI treatment, and the accumulation of antibiotic residues in milk and meat represent significant concerns for public health. Therefore, the investigation of IMI risk factors and the proposal of efficient measures to mitigate their effects on animal health and welfare is crucial. Although fly infestation is considered to play a significant role in the transmission of IMI pathogens, its adverse effects on udder health and the overall comfort status of dairy ewes have not been quantified and assessed on an evidential basis. Hence, the objectives of this study were to assess, for the first time, the fly repellent effect of deltamethrin and link it to: (i) the occurrence of common bacterial IMI; (ii) the somatic cell counts in milk; and (iii) the serum cortisol and creatine kinase levels (stress and fatigue indicators). The study was carried out in an intensive dairy sheep farm in northern Greece, during peak fly season. Deltamethrin treatment was associated with a reduced (i) number of flies (mostly Musca domestica) landing on treated ewes, compared to untreated ones (p < 0.05); (ii) colony-forming units in the case of Non-aureus Staphylococci IMIs (p < 0.05); and (iii) number of somatic cells in the milk (p < 0.001). Finally, serum cortisol and creatine kinase levels were significantly lower in deltamethrin-treated ewes (p < 0.001), indicating a less stressful environment for them.

scholarly journals Prevalence of coagulase-negative staphylococci in bovine mastitis in Zimbabwe

2002 ◽  
Vol 73 (2) ◽  
Author(s):  
T. Kudinha ◽  
C. Simango
Keyword(s):  
Somatic Cell ◽  
Enteric Bacteria ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Bacillus Species ◽  
Small Scale ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts

This study was carried out to determine the prevalence of coagulase-negative staphylococci in clinical and subclinical mastitis in commercial and small-scale farms in Zimbabwe. Thirty five quarter milk samples from clinical mastitis cases and 371 quarter milk samples from cows with subclinical mastitis were cultured for bacterial pathogens. The most frequent pathogens isolated in clinical mastitis were the enteric bacteria (31.4 %), followed by coagulase negative staphylococci (22.9 %) and then Staphylococcus aureus (17.1 %), whereas in subclinical mastitis S. aureus (34.2 %) and coagulase-negative staphylococci were (33.2 %) the most common. Bacillus species were only isolated in milk samples from subclinical mastitis. Coagulase-negative staphylococci were observed in mixed infections with other bacteria in only 2.2 % of the 406 milk samples from clinical and subclinical mastitis where they were isolated together with Bacillus species in 6 of the 9 mixed infection cases. About 95 % of the milk samples from which 131 coagulase-negative staphylococci were isolated had correspondingly high somatic cell counts. The coagulase-negative staphylococci isolated most frequently were S. chromogenes (7.9 %), S. epidermidis (7.4 %) and S. hominis (5.9 %). They were all associated with high somatic cell counts. All the coagulase-negative staphylococci isolates were susceptible to cloxacillin and erythromycin, and more than 90 %of the isolates were susceptible to neomycin, penicillin and streptomycin. The highest resistance was to tetracycline (17.6 %), followed by lincomycin (13.7 %). About 8 % of the isolates were resistant to both penicillin and streptomycin.

Evaluation of diagnostic procedures for subclinical mastitis in meat-producing sheep

2003 ◽  
Vol 70 (2) ◽  
pp. 139-148 ◽  
Author(s):  
Archie CA Clements ◽  
David J Taylor ◽  
Julie L Fitzpatrick
Keyword(s):  
Diagnostic Procedures ◽  
Subclinical Mastitis ◽  
Gel Formation ◽  
Atmospheric Conditions ◽  
Operating Characteristics ◽  
California Mastitis Test ◽  
Clinically Normal ◽  
Cell Counts ◽  
Incubation Temperatures

Samples of foremilk were collected from 261 clinically normal glands of 150 ewes, and tested using the California mastitis test (CMT). Further samples were collected from 195 of these glands for determination of automated somatic cell counts (SCC), and from 60 of these glands for bacteriological assessment. The sensitivity and specificity of CMT for detecting samples with SCC above different threshold levels and for CMT and SCC in determining bacteriological status were evaluated using two-graph receiver operating characteristics (TG-ROC). Milk samples were obtained subsequently from ten CMT positive, and five CMT negative first- and second-lactation ewes. Samples were cultured using a variety of media, incubation temperatures and atmospheric conditions, immediately after collection, and 1 week after storage at 4°C and −21°C. Results suggested that CMT is best used as a diagnostic test for ovine subclinical mastitis (SCM) with a cut-off of 3 (distinct gel formation), and that automated SCC thresholds of >1200×103 cells/ml are appropriate, especially where low prevalences are expected (e.g. <5%). Additionally, this study showed that routine bacteriological methods were appropriate for isolation of most species of pathogen responsible for ovine SCM, but storage of samples prior to culture, either at 4°C or −21°C, was detrimental to the isolation of several of these organisms.

Using dairy herd improvement records and clinical mastitis history to identify subclinical mastitis infections at dry-off

2008 ◽  
Vol 75 (2) ◽  
pp. 240-247 ◽  
Author(s):  
Audrey H Torres ◽  
Päivi J Rajala-Schultz ◽  
Fred J DeGraves ◽  
Kent H Hoblet
Keyword(s):  
Dairy Herd ◽  
Cost Effective ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Somatic Cell Counts ◽  
Dry Cow Therapy ◽  
Cell Counts ◽  
On Farm ◽  
First 90 Days ◽  
Major Pathogens

Interest in selective dry cow therapy (SDCT) has been increasing owing to concerns over development of antimicrobial resistance. Implementation of SDCT, however, requires a quick and cost-effective on-farm method for identifying cows for treatment and cows that can be left without treatment. The objective of the present study was to evaluate the use of clinical mastitis (CM) history and somatic cell counts (SCC) from monthly Dairy Herd Improvement (DHI) records in identification of infected and uninfected cows at dry-off. A total of 647 Holstein cows were classified as uninfected or infected at dry-off based on CM history and varying number of monthly SCC records (with three different SCC cut-offs). Cows were considered uninfected based on the following criteria: (1) SCC <100 000 cells/ml and no CM during the lactation; (2) SCC <200 000 cells/ml and no CM during the lactation; (3) as criterion two, but additionally a cow was also considered uninfected if it experienced a case of CM during the first 3 months of the lactation and the SCC was <100 000 cells/ml for the rest of the lactation; (4) SCC <300 000 cells/ml and no CM during the lactation; otherwise they were considered infected. Infected and uninfected cows at dry-off were most efficiently identified using three months' SCC records with a threshold of 200 000 cells/ml for cows without CM during the lactation and a threshold of 100 000 cells/ml during the rest of lactation for cows with CM during the first 90 days in milk. Moreover, this criterion also most efficiently identified cows infected with major pathogens only at dry-off. The success of the criteria used for identifying infected and uninfected cows will, however, depend on herd characteristics, such as prevalence of infection and type of pathogens present in the herd.

scholarly journals Application of the Support Vector Machine to Predict Subclinical Mastitis in Dairy Cattle

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Nazira Mammadova ◽  
İsmail Keskin
Keyword(s):  
Support Vector Machine ◽  
Dairy Cattle ◽  
Somatic Cell ◽  
Control Period ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Support Vector ◽  
Cross Sectional ◽  
Somatic Cell Counts ◽  
Cell Counts

This study presented a potentially useful alternative approach to ascertain the presence of subclinical and clinical mastitis in dairy cows using support vector machine (SVM) techniques. The proposed method detected mastitis in a cross-sectional representative sample of Holstein dairy cattle milked using an automatic milking system. The study used such suspected indicators of mastitis as lactation rank, milk yield, electrical conductivity, average milking duration, and control season as input data. The output variable was somatic cell counts obtained from milk samples collected monthly throughout the 15 months of the control period. Cattle were judged to be healthy or infected based on those somatic cell counts. This study undertook a detailed scrutiny of the SVM methodology, constructing and examining a model which showed 89% sensitivity, 92% specificity, and 50% error in mastitis detection.

scholarly journals Endovascular Biopsy: Evaluating the Feasibility of Harvesting Endothelial Cells Using Detachable Coils

2013 ◽  
Vol 19 (4) ◽  
pp. 399-408 ◽  
Author(s):  
Daniel L. Cooke ◽  
Hua Su ◽  
Zhengda Sun ◽  
Yi Guo ◽  
Diana Guo ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Endothelial Cell ◽  
Light Microscopy ◽  
Vascular Tissue ◽  
Cellular Material ◽  
Comparable Efficacy ◽  
Cell Counts ◽  
Detachable Coils ◽  
Endothelial Cell Counts

The absence of safe and reliable methods to harvest vascular tissue in situ limits the discovery of the underlying genetic and pathophysiological mechanisms of many vascular disorders such as aneurysms. We investigated the feasibility and comparable efficacy of endothelial cell collection using a spectrum of endovascular coils. Nine detachable coils ranging in k coefficient (0.15–0.24), diameter (4.0 mm–16.0 mm), and length (8.0 cm–47.0 cm) were tested in pigs. All coils were deployed and retrieved within the iliac artery of pigs (three coils/pig). Collected coils were evaluated under light microscopy. The total and endothelial cells collected by each coil were quantified. The nucleated cells were identified by Wright-Giemsa and DAPI stains. Endothelial and smooth muscle cells were identified by CD31 and α-smooth muscle actin antibody staining. Coils were deployed and retrieved without technical difficulty. Light microscopy demonstrated sheets of cellular material concentrated within the coil winds. All coils collected cellular material while five of nine (55.6%) coils retrieved endothelial cells. Coils collected mean endothelial cell counts of 89.0±101.6. Regression analysis demonstrated a positive correlation between increasing coil diameter and endothelial cell counts (R2 = 0.52, p = 0.029). Conventional detachable coils can be used to harvest endothelial cells. The number of endothelial cells collected by a coil positively correlated with its diameter. Given the widespread use of coils and their well-described safety profile their potential as an endovascular biopsy device would expand the availability of tissue for cellular and molecular analysis.

Subclinical mastitis in dairy cows in Swiss organic and conventional production systems

2006 ◽  
Vol 74 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Markus Roesch ◽  
Marcus G Doherr ◽  
Walter Schären ◽  
Melchior Schällibaum ◽  
Jürg W Blum
Keyword(s):  
Dairy Cows ◽  
Production Systems ◽  
Post Partum ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococci ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Cell Counts ◽  
Conventional Production

The objective was to compare the prevalence of subclinical mastitis (SM) and of udder pathogens in 60 Swiss organic (OP) and 60 conventional production systems (CP). Cows (n=970) were studied for SM prevalence and udder pathogens at median 31 d and 102 d post partum. Cows showing a [ges ]1+ positive California Mastitis Test (CMT) in at least one quarter were considered to have SM. Cow-level prevalences of SM for visits at 31 d and 102 d post partum (39% and 40% in OP and 34% and 35% in CP) were similar, but quarter-level prevalences of SM were higher (P<0·02) in OP than CP (15% and 18% in OP and 12% and 15% in CP). Median somatic cell counts in milk at 31 d post partum were higher (P<0·05) in OP than CP cows (43000 and 28000 cells/ml, respectively), but were similar at 102 d post partum in OP and CP cows (45000 and 38000 cells/ml, respectively). In milk samples from quarters showing a CMT reaction [ges ]2+ the prevalences of coagulase negative staphylococci were lower (P<0·05) at 102 d post partum, whereas prevalences of non-agalactiae streptococci were higher (P<0·05) in OP than in CP cows at 31 d and 102 d post partum. In conclusion, under Swiss conditions, subclinical mastitis is a greater problem in organic than in conventional production systems, but differences are not marked.

Presence of cathelicidin-1 in milk as an indicator of the severity of mammary infection in ewes

2020 ◽  
Vol 17 ◽  
Author(s):  
Angeliki I. Katsafadou ◽  
Natalia G.C. Vasileiou ◽  
George T. Tsangaris ◽  
Katerina S. Ioannidi ◽  
Athanasios K. Anagnostopoulos ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Milk Yield ◽  
Inverse Correlation ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Somatic Cell Counts ◽  
Milk Samples ◽  
Cell Counts ◽  
The Mean ◽  
Important Disease

: Aims: The importance of cathelicidin-1 as an indicator of the severity of mammary infection in ewes. Background: Mastitis is an important disease of sheep, affecting their health and welfare. Objective: The association of the presence of cathelicidin-1 in milk samples from ewes with mastitis with the severity of the infection. Methods: Ewes were intramammarily inoculated with Mannheimia haemolytica or Staphylococcus chromogenes. Conventional (clinical, bacteriological and cytological examinations; milk yield measurements) and proteomics evaluation (2-DE, MALDI-TOF MS) to record cathelicidin-1 spot optical densities in milk samples were recorded. Results: Ewes challenged with M. haemolytica developed clinical and ewes challenged with S. chromogenes subclinical mastitis (P=0.05). The challenge organism was isolated from milk samples from inoculated mammary glands; increased somatic cell counts were also recorded. Cathelicidin-1 was detected in milk samples from the inoculated side of udders of all ewes. Mean spot density of cathelicidin-1 from samples from inoculated glands of ewes challenged with M. haemolytica was higher than from ewes challenged with S. chromogenes: 2896 ± 973 versus 1312 ± 361 (P =0.034). There were significant correlations between the presence of clinical mastitis / somatic cell counts with the spot density of cathelicidin-1 on 2-DE gels (P=0.043 and P=0.023, respectively). There was also a significant inverse correlation between the mean spot densities of cathelicidin-1 in milk samples and the milk yield of respective ewes on D10 (P =0.031). Conclusion: Potentially, cathelicidin-1 could be used as a marker to indicate the severity of damage to the mammary parenchyma.

Isolation and characterization of mastitis pathogens and milk composition changes in Murrah buffaloes (Bubalus bubalis) during winter season

2016 ◽  
Author(s):  
Sunita Thakur ◽  
Mahendra Singh ◽  
Gajendra Kumar Aseri ◽  
Archana Verma ◽  
Sikander S. Khan
Keyword(s):  
Winter Season ◽  
Subclinical Mastitis ◽  
Bubalus Bubalis ◽  
Milk Composition ◽  
Milk Samples ◽  
Murrah Buffaloes ◽  
California Mastitis Test ◽  
Isolation And Characterization ◽  
Cell Counts ◽  
Quality Of Milk

Present investigation was carried out to study the prevalence of bacterial pathogens in clinical and subclinical mastitis in buffaloes during winter season. A total of 118 Murrah buffaloes were screened using California mastitis Test (CMT). Milk samples were collected and analyzed for fat, protein, lactose, SNF, pH, Electrical conductivity (EC) and Somatic Cell Counts (SCC). Out of 118 milk samples, 60 samples (50.84%) were negative for CMT and 58 samples (49.15%) were detected positive for mastitis incidence. Milk pH and EC was significantly higher (p<0.05) in mastitis affected samples than the normal ones, however, protein, fat, SNF and lactose were lower (p<0.05). SCC ranged between 1.28-1.48 x 105 cells/ml (in normal milk samples as compared to 3.85-6.21x 105 cells/ ml (p<0.05) in mastitis milk samples. Out of 58 samples only 51 samples exhibited bacterial growth. The culturally examined and characterized samples revealed S. aureus (35.29%) as the predominant bacteria followed by S. agalactiae with an isolation rate of 25.49%. The incidence of coliforms bacteria was not detected in any of the sample cultured and examined. It was concluded that mastitis incidence adversely affect quality of milk by increasing the SCC, pH and EC of milk. Appropriate measures needs to be taken to prevent the incidence of S. aureus bacteria which was the major causative agent.

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