AKTIVITAS EKSTRAK METANOL DAUN ULIN (EUSIDEROXYLON ZWAGERI TEIJSM & BINN) TERHADAP EMPAT JENIS BAKTERI PATOGEN

Abstrak: Suku Dayak Uud Danum di Kalimantan Barat memanfaatkan daun Ulin (Eusideroxylon zwageri Teijsm & Binn) sebagai tumbuhan obat dalam pengobatan tradisional untuk mengobati berbagai penyakit seperti diare, demam dan penguat badan. Penelitian ini dilakukan untuk menganalisis aktivitas ekstrak metanol dari daun ulin terhadap beberapa jenis bakteri patogen. Daun ulin diekstraksi dengan menggunakan metanol, kemudian diuji dengan metode difusi terhadap empat jenis bakteri yaitu Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi pada empat level konsentrasi (1, 5, 10 dan 15 mg/ml) dan E. coli (50, 100, 150 dan 200 mg/ml). Hasil pengujian memperlihatkan bahwa semua level konsentrasi berpengaruh signifikan terhadap hambatan pertumbuhan bakteri patogen. Semakin tinggi konsentrasi ekstrak metanol daun ulin semakin tinggi diameter hambat yang dihasilkan. Diameter hambat tertinggi ditunjukkan oleh konsentrasi 15 mg/ml pada jenis bakteri S. typhi (12,33 mm) dan E. coli pada konsentrasi 200 mg/ml (22,67 mm). Hasil pengujian membuktikan bahwa daun ulin yang secara tradisional digunakan dalam pengobatan tradisional memiliki potensi sebagai antibakteri alami.Kata kunci: Ulin, antibakteri, Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi, dan Escherichia coli.Abstract: Dayak Uud Danum of West Kalimantan traditionally use ulin leaves (Eusideroxylon zwageri Teisjm & Binn) to cure various diseases namely diarrhea, fever, and tonic. This present study aims to evaluate the activity of methanol extract of ulin leaves against several types of pathogenic bacteria. Ulin leaves were extracted with methanol; thus, the extract resulted tested in terms of disc diffusion Kirby and Bauer against four types of bacteria such as Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi (1, 5, 10, and 15 mg/ml), and Escherichia coli (50, 100, 150, and 200 mg/ml). The results showed that all levels of concentration significantly affect the inhibition growth of pathogenic bacteria. The higher the concentration of methanol extract of ulin leaves used, the higher the inhibition zone produced. The highest response inhibition growth showed from the highest level of 15 mg/ml on S. tyhpi (12.33 mm), and E. coli was 200 mg/ml (22.67 mm). It suggested that the leaves of ulin which traditionally used as medicinal plant have the potency as natural antibacterial agents.Keywords: Ulin, antibacterial, Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi, and Escherichia coli.

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Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus

Jurnal MIPA ◽

10.35799/jm.3.2.2014.5989 ◽

2014 ◽

Vol 3 (2) ◽

pp. 129

Author(s):

Megawati S. Saroinsong ◽

Febby E. F. Kandou ◽

Adelfia Papu ◽

Marina F. O. Singkoh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methanol Extract ◽

Inhibition Zone ◽

E Coli ◽

Activity Test ◽

And Staphylococcus Aureus ◽

Paper Disc ◽

Coli Growth

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.

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Uji Efek Antibakteri Jamur Endofit Akar Bakau Rhizophora stylosa Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

Jurnal e-Biomedik ◽

10.35790/ebm.2.1.2014.3694 ◽

2014 ◽

Vol 2 (1) ◽

Author(s):

Michele Sumampouw

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Endophytic Fungi ◽

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Inhibition Zone ◽

Potato Dextrose Agar ◽

Rhizophora Stylosa ◽

E Coli

Abstract: The aim of this study is to investigate the antibacterial effect of endophytic fungi originated from the root of mangrove Rhizophora stylosa growing on Mangrove Plantation Area around DAS Simpang 5 Jl. Piere Tendean Manado. The method of this research was using combination of PDA and NA media. Two species of endophytic fungi were extracted from the root of R. stylosa, black mycelium fungi as isolate A and brown mycelium fungi as isolate B. The activities of both isolates were tested against pathogenic bacteria Staphylococcus aureus and Escherichia coli. The result showed the same inhibition zone of isolate A and B to S. aureus, while, isolate A showed bigger the inhibition zone in comparison to isolate B against E. coli. As a conclusion, the endophytic fungi taken from the root of R. stylosa have antibacterial activity toward S. aureus and E. coli. Keywords: endophytic fungi, Rhizophora stylosa, antibacterial, Staphylococcus aureus, Escherichia coli. Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari jamur endofit yang diambil dari akar bakau Rhizophora stylosa yang ditanam di sekitar Perairan Daerah Aliran Sungai Simpang Lima Jl. Piere Tendean Manado. Metode penelitian dilakukan dengan menggunakan kombinasi media Potato Dextrose Agar (PDA) dan Nutrient Agar (NA). Dari akar bakau R. stylosa diperoleh dua jenis jamur endofit yaitu isolat A dengan karateristik miselium jamur berwarna hitam dan isolat B yang dengan karakteristik miselium berwarna coklat. Kedua isolat ini selanjutnya diujikan aktivitasnya terhadap bakteri Staphylococcus aureus dan Escherichia coli. Hasil penelitian menunjukkan isolat A dan B memberikan zona hambat yang sama terhadap bakteri Staphylococcus aureus dan pada bakteri Escherichia coli isolat A menunjukkan diameter zona hambat yang lebih besar dibandingkan isolat B. Kesimpulan, Jamur endofit yang diisolasi dari akar bakau R. Stylosa memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan E. coli. Kata kunci: jamur endofit, Rhizophopra stylosa, antibakteri, Staphylococcus aureus, Escherichia coli.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI SPONS Leucetta chagosensis DARI PERAIRAN PULAU MANTEHAGE SULAWESI UTARA TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽

10.35799/pha.10.2021.34032 ◽

2021 ◽

Vol 10 (2) ◽

pp. 834

Author(s):

Eunike Pelealu ◽

Defny S. Wewengkang ◽

Surya Sumantri Abdullah

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Bacterial Growth ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Disk Diffusion ◽

E Coli ◽

Activity Test ◽

Escherichia Coli Bacteria ◽

Metabolite Content

ABSTRACTSponges are one of the biota components that make up coral reefs which are quite widely distributed. The metabolite content in the sponge can ward off and inhibit the pathogenic bacteria that interfere with it. This study aims to determine the activity of inhibiting bacterial growth from the extract and fraction of Leucetta chagosensis sponge against the growth of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The samples were extracted using the maceration method with 95% ethanol solvent and then fractionated using 3 solvents with different polarity levels, namely methanol, n-hexane and chloroform. Activity test using the disk diffusion agar method of Kirby and Bauer. Only the MeOH fraction was able to inhibit the growth of E. coli bacteria with an average inhibition zone of 6.88 mm. Whereas for S.aureus bacteria extracts and all fractions showed activity to inhibit bacterial growth with an average inhibition zone of EtOH (6.61 mm), CHCI3 (6.68 mm), n-hexane (7.83 mm) and MeOH (8.00 mm), respectively. All activities that are shown are categorized as weak (weak).Keywords: Antibacterial, Leucetta chagosensis, Staphylococcus aureus, Escherichia coli ABSTRAKSpons merupakan salah satu komponen biota penyusun terumbu karang yang penyebarannya cukup luas. Kandungan metabolit yang ada di dalam spons dapat menangkal dan menghambat bakteri patogen pengganggunya. Penelitian ini bertujuan untuk melihat aktivitas menghambat pertumbuhan bakteri dari ekstrak dan fraksi spons Leucetta chagosensis. terhadap pertumbuhan bakteri Gram positif Staphylococcus aureus dan Gram negatif Escherichia coli. Sampel di ekstraksi menggunakan metode maserasi dengan pelarut etanol 95% lalu di fraksinasi dengan menggunakan 3 pelarut dengan tingkat kepolaran yang berbeda yaitu metanol, n-heksan dan kloroform. Uji aktivitas menggunakan metode disk diffusion agar Kirby dan Bauer. Hanya fraksi MeOH yang mampu menghambat pertumbuhan bakteri E.coli dengan zona hambat rata-rata 6,88 mm. Sedangkan terhadap bakteri S.aureus ekstrak dan semua fraksi menunjukan aktifitas menghambat pertumbuhan bakteri dengan rata-rata zona hambat masing-masing EtOH (6,61 mm), CHCl3 (6,68 mm), n-Heksan (7,83 mm), dan MeOH (8,00 mm). Semua aktivitas yang ditunjukan dikategorikan lemah (weak).Kata kunci : Antibakteri, Leucetta chagosensis, Staphylococcus aureus, Escherichia coli

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Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli. (Antibacterial Test of Chitosan Nanoparticles against Staphylococcus Aureus and Escherichia coli)

JURNAL BIOS LOGOS ◽

10.35799/jbl.10.1.2020.27652 ◽

2020 ◽

Vol 10 (1) ◽

pp. 7

Author(s):

Alce Magani ◽

Trina Tallei ◽

Beivy Kolondam

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Chitosan Nanoparticles ◽

Inhibition Zone ◽

Antibacterial Test ◽

E Coli ◽

Inhibition Of Growth ◽

Significant Difference ◽

One Way Anova

Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli.(Antibacterial Test of Chitosan Nanoparticles against Staphylococcus Aureus and Escherichia coli) Alce K. Magani*, Trina E. Tallei, Beivy J. KolondamProgram Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] (Article History: Received 30-12-2019; Revised 15-01-2020; Accepted 23-01-2020) Abstrak Antibakteri merupakan zat yang dapat menghambat pertumbuhan bakteri dan dapat membunuh bakteri penyebab infeksi. Staphylococcus aureus dan Escherichia coli merupakan bakteri Gram positif dan Gram negatif yang dapat menimbulkan infeksi atau penyakit dalam tubuh. Penelitian ini bertujuan untuk menguji aktivitas bakteri patogen dengan memakai nanopartikel kitosan sebagai antibakteri yang dibuat dalam empat konsentrasi (0,5%, 1%, 1,5% dan 2%) serta penggunaan kontrol asam asetat 1%, ciprofloxacin dan air steril sebagai pembanding. Metode penelitian yang digunakan yaitu metode gelasi ionik untuk pembuatan nanopartikel kitosan dan difusi agar untuk pengujian antibakteri. Data dianalisis dengan One Way Anova yang dilanjutkan dengan metode BNT (Beda Nyata Terkecil). Hasil penelitian diperoleh penghambatan pertumbuhan bakteri S. aureus dan E. coli tertinggi pada konsentrasi 0,5%, dengan diameter zona hambat hari pertama sampai hari ketiga 12,31 mm, 9,98 mm, dan 20,46 mm pada S. aureus dan 15,88 mm, 18,71 mm, dan 20,43 mm pada E. coli, kategori kuat, dan bersifat bakteriostatik dan penghambatan terendah pada konsentrasi 2% dengan diameter zona hambat pada S. aureus yaitu 5,56 mm, 5,50 mm, dan 5,40 mm, dan pada E. coli yaitu 5,93 mm, 9,64 mm, dan 12,58 mm, kategori sedang, dan bersifat bakteriostatik. Kata kunci: Kitosan, nanopartikel kitosan, aktivitas antibakteri. Abstract Antibacteria is a substance that can inhibit the growth of bacteria and able to kill bacteria that cause infections. Staphylococcus aureus and Escherichia coli are Gram positive and Gram negative bacteria that able to cause infections or diseases. This study aimed to examine the activity of pathogenic bacteria by using chitosan nanoparticles as antibacterial. The treatments were made in four concentrations (0.5%, 1%, 1.5% and 2%) and, for comparison, there were also acetic acid control, ciprofloxacin and sterile water. The research method used is the ionic gelation method for the manufacture of chitosan nanoparticles and agar diffusion for antibacterial testing. Data were analyzed with One Way Anova followed by LSD (Least Significant Difference) method. The results showed the highest inhibition of growth of S. aureus and E. coli bacteria at a concentration of 0.5%, with a diameter of inhibition zones of the first day to the third day of 12.31 mm, 9.98 mm, and 20.46 mm in S. aureus and 15,88 mm, 18,71 mm, and 20,43 mm in E. coli, the strong category, and are bacteriostatic and the lowest inhibition was at 2% concentration with inhibition zone diameters in S. aureus namely 5.568 mm, 5.50 mm, and 5, 40 mm, and in E. coli, 5.93 mm, 9.63 mm and 12.58 mm, the medium category and bacteriostatic.Key words: Chitosan, nanoparticles chitosan, antibacterial activity.

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Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli.

JURNAL BIOS LOGOS ◽

10.35799/jbl.10.1.2020.27978 ◽

2020 ◽

Vol 10 (1) ◽

pp. 7

Author(s):

Alce K Magani ◽

Trina E Tallei ◽

Beivy J Kolondam

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Chitosan Nanoparticles ◽

Inhibition Zone ◽

E Coli ◽

Inhibition Of Growth ◽

Significant Difference ◽

Testing Data ◽

One Way Anova

Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli.(Antibacterial Test of Chitosan Nanoparticles against Staphylococcus aureus and Escherichia coli) Alce K. Magani*, Trina E. Tallei, Beivy J. KolondamProgram Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] (Article History: Received 30-12-2019; Revised 15-01-2020; Accepted 23-01-2020) Abstrak Antibakteri merupakan zat yang dapat menghambat pertumbuhan bakteri dan dapat membunuh bakteri penyebab infeksi. Staphylococcus aureus dan Escherichia coli merupakan bakteri Gram positif dan Gram negatif yang dapat menimbulkan infeksi atau penyakit dalam tubuh. Penelitian ini bertujuan untuk menguji aktivitas bakteri patogen dengan memakai nanopartikel kitosan sebagai antibakteri yang dibuat dalam empat konsentrasi (0,5%, 1%, 1,5% dan 2%) serta penggunaan kontrol asam asetat 1%, ciprofloxacin dan air steril sebagai pembanding. Metode penelitian yang digunakan yaitu metode gelasi ionik untuk pembuatan nanopartikel kitosan dan difusi agar untuk pengujian antibakteri. Data dianalisis dengan One Way Anova yang dilanjutkan dengan metode BNT (Beda Nyata Terkecil). Hasil penelitian diperoleh penghambatan pertumbuhan bakteri S. aureus dan E. coli tertinggi pada konsentrasi 0,5%, dengan diameter zona hambat hari pertama sampai hari ketiga 12,31 mm, 9,98 mm, dan 20,46 mm pada S. aureus dan 15,88 mm, 18,71 mm, dan 20,43 mm pada E. coli, kategori kuat, dan bersifat bakteriostatik dan penghambatan terendah pada konsentrasi 2% dengan diameter zona hambat pada S. aureus yaitu 5,56 mm, 5,50 mm, dan 5,40 mm, dan pada E. coli yaitu 5,93 mm, 9,64 mm, dan 12,58 mm, kategori sedang, dan bersifat bakteriostatik. Kata kunci: Kitosan, nanopartikel kitosan, aktivitas antibakteri. Abstract Antibacteria is a substance that can inhibit the growth of bacteria and able to kill bacteria that cause infections. Staphylococcus aureus and Escherichia coli are Gram positive and Gram negative bacteria that able to cause infections or diseases. This study aimed to examine the activity of pathogenic bacteria by using chitosan nanoparticles as antibacterial. The treatments were made in four concentrations (0.5%, 1%, 1.5% and 2%) and, for comparison, there were also acetic acid control, ciprofloxacin and sterile water. The research method used is the ionic gelation method for the manufacture of chitosan nanoparticles and agar diffusion for antibacterial testing. Data were analyzed with One Way Anova followed by LSD (Least Significant Difference) method. The results showed the highest inhibition of growth of S. aureus and E. coli bacteria at a concentration of 0.5%, with a diameter of inhibition zones of the first day to the third day of 12.31 mm, 9.98 mm, and 20.46 mm in S. aureus and 15,88 mm, 18,71 mm, and 20,43 mm in E. coli, the strong category, and are bacteriostatic and the lowest inhibition was at 2% concentration with inhibition zone diameters in S. aureus namely 5.568 mm, 5.50 mm, and 5, 40 mm, and in E. coli, 5.93 mm, 9.63 mm and 12.58 mm, the medium category and bacteriostatic.Key words: Chitosan, nanoparticles chitosan, antibacterial activity.

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AKTIVITAS ANTIBAKTERI EKSTRAK METANOL BATANG BIDARA LAUT (Strychnos ligustrina) TERHADAP BAKTERI PATOGEN

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v19i1.1040 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Edy Kurniawan ◽

Dwi Soelistya Dyah Jekti ◽

Lalu Zulkifli

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Klebsiella Pneumonia ◽

E Coli

Abstract : Strychnos ligustrina stem has been empirically used by the people of West Nusa Tenggara and Bali in the treatment of malaria, tooth ache and diarrhea, but there is no scientific data that supports it. This study aims to determine and prove the antibacterial activity of Strychnos ligustrina methanol extract to pathogenic bacteria in vitro and in vivo. This research is an experimental study conducted by measuring the inhibition zone (mm) growth of pathogenic bacteria, determining minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) in vitro, and determining the percentage of antibacterial activity of methanol extract of S. ligustrina stem in vivo. The experiment was conducted using 4 groups of concentrations of S. ligustrina stem methanol extract in an in vitro study of 25, 50, 75, and 100% with ciprofloxacin as a positive control and aquadest as a negative control. In vivo studies experiments were carried out using 6 treatment groups of test animals male mice Balb / c (Mus musculus). The in vitro test results showed that methanol extract of S. ligustrina stems was able to inhibit the growth of pathogenic bacteria with medium categories of clinical isolates of Staphylococcus aureus and categories of weaks to Klebsiella pneumonia and Escherichia coli isolates. The minimum inhibitory concentration (MIC) for S. aureus and K. pneumonia bacteria isolates was at a concentration of 25% while for E. coli isolates at a concentration of 30%. The methanol extract of the S. ligustrina stem has no killing power against the pathogenic bacteria tested. Antibacterial activity in vivo was able to inhibit the growth of S. aureus pathogenic bacteria by 6.60% (at 25% concentration), 8.62% (at 50% concentration), and 17.31% (at 100% concentration), against K. pneumonia was 11.85% (at 25% concentration), 51.21% (at 50% concentration), and 65.92% (at 100% concentration), against E. coliat 19.18% (at concentration 25%), 29.98% (at 50% concentration), and 40.88% (at 100% concentration). Methanol extract of S. ligustrina stem proved to have antibacterial activity in vitro and in vivo. Key words: Srychnos ligustrina, pathogenic bacteria, antibacterial, in vitro, in Vivo. Abstrak : Strychnos ligustrina secara empiris telah digunakan oleh masyarakat Nusa Tenggara Barat dan Bali dalam pengobatan penyakit malaria, sakit gigi, dan diare, tetapi belum ada data ilmiah yang mendukung. Penelitian ini bertujuan untuk menentukan dan membuktikan aktivitas antibakteri ekstrak metanol batang bidara laut terhadap bakteri patogen secara in vitrodan in vivo. Penelitian ini merupakan penelitian eksperimental yang dilakukan dengan mengukur zona hambat (mm) pertumbuhan bakteri patogen, menentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) secara in vitro, serta menentukan persentase aktivitas antibakteri ekstrak metanol batang bidara laut secara in vivo. Percobaan dilakukan menggunakan 4 kelompok konsentrasi ekstrak metanol batang bidara laut pada penelitian in vitro yaitu 25, 50, 75, dan 100% dengan ciprofloxacin sebagai kontrol positif serta aquadest sebagai kontrol negatif. Pada penelitian in vivo percobaan dilakukan menggunakan 6 kelompok perlakuan hewan uji mencit jantan galur Balb/c (Mus musculus). Hasil uji in vitro menunjukkan ekstrak metanol batang bidara laut mampu menghambat pertumbuhan bakteri patogen dengan kategori sedang terhadap Staphylococcus aureus isolat klinis dan kategori lemah terhadap Klebsiella pneumonia dan Escherichia coli isolat klinis. Nilai konsentrasi hambat minimum (KHM) untuk isolat bakteri S. aureus dan K. pneumoniae adalah pada konsentrasi 25% sedangkan untuk isolat E. coli pada konsentrasi 30%. Ekstrak metanol batang bidara laut tidak memiliki daya bunuh terhadap bakteri patogen yang diuji. Aktivitas antibakteri secara in vivo mampu menghambat pertumbuhan bakteri patogen S. aureus sebesar 6,60% (pada konsentrasi 25%), 8,62% (pada konsentrasi 50%), dan 17,31% (pada konsentrasi 100%), terhadap K. pneumonia sebesar 11,85% (pada konsentrasi 25%), 51,21% (pada konsentrasi 50%), dan 65,92% (pada konsentrasi 100%), terhadap E. coli sebesar 19,18% (pada konsentrasi 25%), 29,98% (pada konsentrasi 50%), dan 40,88% (pada konsentrasi 100%). Ekstrak metanol batang bidara laut terbukti memiliki aktivitas antibakteri secara in vitro dan in vivo. Kata kunci: Srychnos ligustrina, bakteri patogen, antibakteri, in vitro, in vivo

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UJI DAYA HAMBAT EKSTRAK METANOL Selaginella delicatula DAN Diplazium dilatatum TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

JURNAL ILMIAH SAINS ◽

10.35799/jis.15.1.2015.8238 ◽

2015 ◽

Vol 15 (1) ◽

pp. 52 ◽

Cited By ~ 2

Author(s):

Stery B. Oroh ◽

Febby E.F. Kandou ◽

Johanis Pelealu ◽

Dingse Pandiangan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Methanol Extract ◽

Base Material ◽

Inhibition Zone ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Inhibition Zone Diameter ◽

E Coli ◽

Zone Diameter

ABSTRAK Penelitian ini bertujuan untuk menguji daya hambat ekstrak metanol Selaginella delicatula dan Diplazium dilatatum terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Pengujian daya hambat ekstrak terhadap bakteri menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas. Ekstrak S. delicatula menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 6.70 mm dan 7.80 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 8.40 mm dan 13.00 mm. Ekstrak D. dilatatum menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 6.70 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat berturut-turut 0.00 mm, 0.00 mm dan 8.00 mm. Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak S. delicatula dan D. dilatatum tergolong dalam kategori kuat dan sedang serta memiliki potensi sebagai bahan dasar antibakteri, karena kedua ekstrak menunjukkan daya hambatnya. Kata kunci : tumbuhan paku, Selaginella delicatula, Diplazium dilatatum, Antibakteri, Staphylococcus aureus, Escherichia coli INHIBITION TEST OF METHANOL EXTRACT OF Selaginella delicatula AND Diplazium dilatatum AGAINST BACTERIA Staphylococcus aureus AND Escherichiacoli ABSTRACT This study aimed to test the inhibition of methanol extract of Selaginella delicatula and Diplazium dilatatum on the growth of Staphylococcus aureus and Escherichia coli. Testing inhibition of the extracts to bacterial using the Kirby-Bauer method, is the paper disk diffusion method. S. delicatula extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 6.70 mm and 7.80 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 8.40 mm and 13.00 mm. D. dilatatum extract inhibited the growth of S. aureus at concentrations of extract 30%, 60% and 90% inhibition zone diameter respectively 0.00 mm, 0.00 mm and 6.70 mm; and inhibit the growth of E. coli with inhibition zone diameter respectively 0.00 mm, 0.00 mm and 8.00 mm. Based on the results obtained, it can be concluded that the extract of S. delicatula and D. dilatatum classified in the category of medium and strong and has potential as a base material for both extracts showed antibacterial inhibitory power. Keywords: ferns, Selaginella delicatula, Diplazium dilatatum, Antibacterial, Staphylococcus aureus, Escherichia coli.

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UJI AKTIVITAS ANTI BAKTERI EKSTRAK METANOL DAUN BACCAUREA MACROCARPA TERHADAP ESCHERICHIA COLI DAN SALMONELLA TYPHI

Konservasi Hayati ◽

10.33369/hayati.v17i1.12879 ◽

2021 ◽

Vol 17 (1) ◽

pp. 10-16

Author(s):

Vivi Mardina ◽

Fitri Helmalia ◽

Fadhliani Fadhliani ◽

Lendawati Lendawati

Keyword(s):

Escherichia Coli ◽

Methanol Extract ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Positive Control ◽

Negative Control ◽

Optimal Concentration ◽

E Coli ◽

Herbal Plants

Antibiotics are drugs that are used to inhibit bacteria production. The long-term use of antibiotics can cause resistance problems. As a consequent it is necessary to find the alternative antibiotics that are derived from plants. The alternative antibiotics are considered safer, relatively cheap and easy to obtain. One of the herbal plants used is Baccaurea macrocarpa. The objective of this study was to identify the secondary metabolites of B.macrocarpa, the effect of methanol extract from B.macrocarpa leaves on the growth of Escherichia coli and Salmonella typhi bacteria and to measure the optimal concentration of the sample to inhibit the growth of E.coli and S.typhi bacteria. The research has been conducted for 3 months at the Universitas Samudra laboratory. The method used was the well method with E.coli and S.typhi bacteria test. Each treatment of the methanol extract of B. macrocarpa leaves with a concentration of 20%, 30% and 40% (g/mL). Positive control was ciprofloxacin and negative control was sterile distilled water. The results obtained the concentration of 40% (g/mL) as the optimal concentration to inhibit the growth of E. coli and S.typhi bacteria with the resulting inhibition zone of 6.3 mm and 5 mm. The results of the study concluded that the B. Macrocarpa leaf extract could be developed as a basic ingredient for antibiotics.

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Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

Asian Journal of Medical and Biological Research ◽

10.3329/ajmbr.v1i3.26462 ◽

2016 ◽

Vol 1 (3) ◽

pp. 457-462 ◽

Cited By ~ 4

Author(s):

Md Nuruzzaman Munsi ◽

Nathu Ram Sarker ◽

Razia Khatun ◽

Mohammed Khorshed Alam

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pathogenic Bacteria ◽

Bacterial Species ◽

Salmonella Typhi ◽

Diffusion Method ◽

Biochemical Tests ◽

Public Health Importance ◽

Milk Samples ◽

First Choice

Cows milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Grams staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

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Note: Effect of Some Spice Extracts on Bacterial Inhibition

Food Science and Technology International ◽

10.1177/1082013203038976 ◽

2003 ◽

Vol 9 (5) ◽

pp. 353-358 ◽

Cited By ~ 48

Author(s):

O. Sagdic ◽

A. G. Karahan ◽

M. Ozcan ◽

G. Ozkan

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Yersinia Enterocolitica ◽

Inhibition Zone ◽

Bacterial Strains ◽

Inhibitory Effects ◽

Antibacterial Effects ◽

E Coli ◽

Inhibition Zones ◽

Mrs Broth

Eighteen extracts of spices commonly consumed worldwide and grown naturally in Turkey were tested against twenty three bacterial strains to compare their antibacterial effects with eleven antibiotics. Eight pathogens and fifteen lactobacilli isolated from chick intestine were used as the test microorganisms. Pathogens (six different Staphylococcus aureus strains, Escherichia coli ATCC 25922 and Yersinia enterocolitica ATCC 1501) were grown in Nutrient broth and lactobacilli in MRS broth. Hop extracts formed inhibition zones against S. aureus strains of upto 36 mm. Inhibitory effects of hop extracts against S. aureuswere generally higher than that of erythromycin as antibiotic. Helichrysum compactum extract produced an inhibition zone of 23mm to E. coli ATCC 25922 and 26mm to Y. enterocolitica ATCC 1501. Helichrysum compactum extract inhibited the growth of Y. enterocolitica ATCC 1501 more than other spice extracts. While inhibition zones of these extracts against lactobacilli were found smaller than on S. aureus strains, inhibition zones of the same extracts against lactobacilli were found similar to those of E. coli ATCC 25922 and Y. enterocolitica ATCC 1501.

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