scholarly journals Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Md Nuruzzaman Munsi ◽  
Nathu Ram Sarker ◽  
Razia Khatun ◽  
Mohammed Khorshed Alam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Public Health Importance ◽  
Milk Samples ◽  
First Choice

Cow’s milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Gram’s staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

scholarly journals The Inhibition Potentials of Different Honey against Staphylococcus aureus, Escherichia coli and Bacillus species Isolated from Clinical Source

2020 ◽  
pp. 46-55
Author(s):  
Akani, Nedie Patience ◽  
Amadi Wemedo, Samuel ◽  
Njoku, Onyedikachi Egbuchulem
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Inhibition Efficiency ◽  
Minimum Bactericidal Concentration ◽  
Bacterial Species ◽  
Diffusion Method ◽  
Bacillus Species ◽  
Clinical Source

As a result of the increased prevalence of antibiotic resistance among different bacteria, different plants and other natural products have been studied and found to be highly effective against pathogenic bacteria. Honey, over the years has been used as an antibacterial agent to treat certain infections caused by bacteria and is believed to be effective especially in rural areas. This study was thus aimed at comparing the effect of different honey samples against some pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Bacillus cereus) isolated from clinical source. This study was carried out in the microbiology laboratory, department of microbiology Rivers State University Nigeria from January 2018 to August 2019. The antibacterial sensitivity test was carried out using agar well diffusion method while the Minimum inhibitory concentration and Minimum bactericidal concentration were determined using broth tube micro dilution technique in two fold dilution. The inhibition efficiency of the honey samples on the test organisms increased with increasing concentration from 20 to 100% as 100% concentration had the highest zone of inhibition. Staphylococcus aureus (6.33 mm – 26.33 mm) was the most sensitive to the honey samples while Bacillus cereus (0.00 – 19.67 mm) was less sensitive. At concentrations of 20 – 80%, raw and Rowse honey were more effective on E. coli compared to Princenic Global honey, while at 100%, Princenic Global honey was more effective on Staphylococcus aureus. Raw and Rowse honey were more effective at 20 -60% concentrations followed by Princenic Global honey; whereas at 80 -100% concentrations, Raw and Princenic Global honey were more effective. Bacillus cereus was resistancet to the honey samples at 20 – 60% but sensitive at 80 – 100% concentrations to Rowse, Raw and Princenic Global honey. The inhibition efficiency of the honey samples on the growth of the tested organisms was found to be dependent on the concentration and type of honey used, as well as they type of organism tested. The result of the minimum inhibitory and minimum bactericidal concentration showed that Staphylococcus aureus was inhibited most at a lower concentration of 25% compared to other bacteria isolates. All honey samples tested did not show any bactericidal effect but was bacteriostatic to some of the tested organisms. Pharmacological standardization and clinical evaluation on the effect of honey is essential before honey can be used as a preventive and curative measure to common diseases related to the tested bacterial species.

scholarly journals Investigation of the antimicrobial activity of danofoloxacin against bacteria pathogens in goats

2020 ◽  
Vol 22 (97) ◽  
pp. 74-78
Author(s):  
T. I. Stetsko ◽  
Ya. M. Liubenko ◽  
V. N. Padovskyi ◽  
L. L. Ostrovska ◽  
O. Yo. Kalinina ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
Chemotherapeutic Agents ◽  
Diffusion Method ◽  
Bacteriostatic Activity ◽  
Intestinal Infection ◽  
Sensitivity Testing ◽  
First Choice

Fluoroquinolones are critical antimicrobials for both human and veterinary medicine. Due to their unique mechanism of antimicrobial action and good pharmacokinetic properties, they are often the first choice drugs in the treatment of bacterial infections in animals. The purpose of the investigation was to study the antimicrobial activity of a third-generation fluoroquinolone antibiotic of danofloxacin against bacteria, pathogens of respiratory and intestinal infection in goats. The samples of the nasal outflows (respiratory infection) and fecal masses (intestinal infection) were collected from clinically ill goats for microbiological studies. The sensitivity test of the microflora of the biomaterial, carried out by the disco-diffusion method, showed that the microorganisms of all the samples were sensitive to danofloxacin. Bacteria Streptococcus pneumonia (n = 10), Staphylococcus aureus (n = 4) and Escherichia coli (n = 2) were isolated and identified from nasal exudate samples (n = 10). Pathogenic strains of Escherichia coli were isolated from all faecal samples (n = 12). The degree of bacteriostatic activity of danofloxacin was determined by establishing its minimum inhibitory concentration (MIC) for bacterial isolates by sequential dilutions in a liquid nutrient medium. The average MIC of danofloxacin for Streptococcus pneumoniae isolates was 0.26 ± 0.13 μg/ml and for Staphylococcus aureus isolates – 0.25 ± 0.075 μg/ml. For Escherichia coli strains isolated from faeces of goats suffering from coli infection, the average MIC of danofloxacin was 0.38 ± 0.12 μg/ml (range 0.2 to 0.8 μg/ml). Antimicrobial sensitivity testing have shown a high level of bacteriostatic activity of danofloxacin against bacteria, pathogens of respiratory and intestinal infections in goats. This may be the argument for the use of danofloxacin-based chemotherapeutic agents in the treatment of bacterial infections in goats, especially for the empirical approach to therapy.

scholarly journals Bacterial Etiology of Wound Exudates in Tertiary Care Cancer Patients and Antibiogram of the Isolates

2020 ◽  
Vol 13 ◽  
pp. 117863372095207
Author(s):  
Kiran Duwadi ◽  
Sujan Khadka ◽  
Sanjib Adhikari ◽  
Sanjeep Sapkota ◽  
Pabitra Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Methicillin Resistance ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Biochemical Tests ◽  
Cross Sectional

Introduction: Patients with malignancies frequently develop infections as a result of surgical procedures and fungating wounds leading to pus formation. This cross-sectional study was conducted to explore the bacteriological spectra of infections of various cancer sites and their antibiotic sensitivity patterns among the patients visiting minor operation theatre (OT) of B.P. Koirala Memorial Cancer Hospital (BPKMCH), Chitwan, Nepal. Methods: Over a period of 3 months from September to November 2018, a total of 183 wound exudates and pus samples were collected and analyzed by standard microbiological procedures. Isolates were identified based on the colony characters, Gram staining and an array of biochemical tests. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion technique according to criteria set by CLSI, 2016. Methicillin resistance in Staphylococcus aureus was tested with the help of cefoxitin using disc diffusion method. Results: Out of the 183 samples, 149 (81.4%) were culture positive. Among 13 different isolates identified, S. aureus (43.0%) was predominant followed by E. coli (14.0%). Higher incidence of bacteria was seen among the males (52.3%), in the age group 51 to 60 years (26.8%) and among the patients undergoing surgical intervention to deal with cancer (34.2%). The prevalence of wound infection was significantly affected by gender, age, and treatment regimen ( P < .01). Out of the total 68 S. aureus isolates, 38 (44.1%) were deemed as Methicillin-resistant Staphylococcus aureus (MRSA). Among the 158 isolates, 85 (53.8%) were multi-drug resistant (MDR). Cefepime was the most effective antibiotic for Gram positive isolates whereas both imipenem and meropenem were found to be equally more effective for Gram negative isolates. Conclusion: This study suggests that patients with malignancies harbor pathogenic bacteria; therefore, prudent use of antibiotics is essential to prevent the emergence of MDR pathogens.

scholarly journals Molecular characterization of Escherichia coli isolated from milk samples with regard to virulence factors and antibiotic resistance

2021 ◽  
pp. 2410-2418
Author(s):  
Waleed Younis ◽  
Sabry Hassan ◽  
Hams M. A. Mohamed
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
16S Rrna ◽  
Virulence Factors ◽  
Pathogenic Bacteria ◽  
Human Life ◽  
Raw Milk ◽  
Biochemical Tests ◽  
E Coli ◽  
Milk Samples

Background and Aim: Raw milk is considered an essential source of nutrition during all stages of human life because it offers a valuable supply of protein and minerals. Importantly, milk is considered a good media for the growth and contamination of many pathogenic bacteria, especially food-borne pathogens such as Escherichia coli. Thus, the objective of this study was to characterize E. coli and detect its virulence factors and antibiotic resistance from raw milk samples. Materials and Methods: Raw milk samples (n=100) were collected from different localities in Qena, Egypt, and investigated for the presence of E. coli using different biochemical tests, IMViC tests, serotyping to detect somatic antigen type, and molecularly by polymerase chain reaction (PCR) tests. The presence of different virulence and antimicrobial genes (hly, eae, stx1, stx2, blaTEM, tetA(A), and tetB genes) in E. coli isolates was evaluated using PCR. Results: The results demonstrated that 10 out of 100 milk samples were contaminated with E. coli. Depending on serology, the isolates were classified as O114 (one isolate), O27 (two isolates), O111 (one isolate), O125 (two isolates), and untypeable (five isolates) E. coli. The sequencing of partially amplified 16S rRNA of the untypeable isolates resulted in one isolate, which was initially misidentified as untypeable E. coli but later proved as Enterobacter hormaechei. Moreover, antibacterial susceptibility analysis revealed that nearly all isolates were resistant to more than 3 families of antibiotics, particularly to β-lactams, clindamycin, and rifampin. PCR results demonstrated that all E. coli isolates showed an accurate amplicon for the blaTEM and tetA(A) genes, four isolates harbored eae gene, other four harbored tetB gene, and only one isolate exhibited a positive stx2 gene. Conclusion: Our study explored vital methods for identifying E. coli as a harmful pathogen of raw milk using 16S rRNA sequencing, phylogenetic analysis, and detection of virulence factors and antibiotic-resistant genes.

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN SPONS Phyllospongia lamellose SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 252
Author(s):  
Sela S Lempoy ◽  
Widya A Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Isolation And Purification ◽  
Esherichia Coli ◽  
Identification Stage

ABSTRACT Sponges are one source of producing bioactive components from the sea. Bacteria Symbiosis with sponges are thought to have the potential to produce bioactive compounds that have been isolated from sponges. One of the potential of bioactive compounds which have been found and developed from sponges was antibacterial. The aim of this study was to determine the antibacterial activity of bacteria associated with Phyllospongia lamellose sponges against pathogenic bacteria namely Staphylococcus aureus and Esherichia coli and then proceed with identification of biochemistry in isolates which showed the greatest inhibitory activity. Three isolates of sponges symbiont bacteria were obtained through the isolation and purification stage which were then followed by testing of antibacterial activity with paper disk diffusion method. The antibacterial test results showed the diameter of the inhibitory zone against Staphylococcus aureus, were: SL1(8.67 mm), SL2 (9.33 mm) and SL3(9.00 mm) categorized as medium. Whereas the inhibiotion zone shown on Esherichia coli bacteria is also classified as medium, were: SL1(9.67 mm), SL2 (9.00 mm) and SL3 (9.33 mm). The three isolates continued to the identification stage biochemically. Each isolation was assumed as follows: Desulfotomaculum (SL1), Brochothrix (SL2) and Sulfidobacillus (SL3). Keyword         :Phyllospongia lamellose, isolation, biochemically identification ABSTRAK Spons merupakan salah satu sumber penghasil komponen bioaktif yang berasal dari laut. Bakteri yang bersimbiosis dengan spons diduga memiliki potensi dalam menghasilkan senyawa-senyawa bioaktif yang telah diisolasi dari spons. Potensi senyawa bioaktif yang telah ditemukan dan dikembangkan dari spons salah satunya ialah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri dari bakteri yang berasosiasi dengan spons Phyllospongia lamellose terhadap bakteri patogen yakni Staphylococcus aureus dan Escherichia coli dan kemudian dilanjutkan dengan identifikasi secara biokimia pada isolat yang menunjukkan daya hambat terbesar. Diperoleh 3 isolat bakteri simbion spons melalui tahap isolasi dan purifikasi yang kemudian dilanjutkan dengan pengujian aktivitas antibakteri melalui metode difusi kertas cakram. Hasil uji antibakteri menunjukkan diameter zona hambat terhadap bakteri Staphylococcus aureus yaitu: SL1(8.67 mm), SL2(9.33 mm) dan SL3 (9.00 mm) termasuk dalam kategori sedang. Sedangkan zona hambat yang ditunjukkan terhadap bakteri Escherichia coli juga tergolong kategori sedang yaitu: SL1(9.67 mm), SL2(9.00 mm) dan SL3 (9.33 mm). Ketiga isolat dilanjutkan ke tahap identifikasi secara biokimia. Masing-masing isolat diduga sebagai berikut: Desulfotomaculum (SL1), Brochothrix (SL2) dan Sulfidobacillus (SL3). Kata kunci          :Phyllospongia lamellose, isolasi, identifikasi biokimia.

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA Turbinaria ornata (Turner) J. Agardh SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 351
Author(s):  
Cicilia Kosasi ◽  
Widya A. Lolo ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Genus Bacillus ◽  
Biochemical Tests ◽  
Isolation And Purification ◽  
Turbinaria Ornata ◽  
Gram Staining ◽  
Escherichia Coli Bacteria

ABSTRACT Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test,  tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed  that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J.   Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya  sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.

scholarly journals Antibacterial Activity of Zingiber officinale (Ginger) against Clinical Bacterial Isolates

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Walla Fadel Mohammed ◽  
Bushra Hindi Saleh ◽  
Reem Naem Ibrahim ◽  
Mohammed Bdaiwy Hassan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Therapeutic Potential ◽  
Morphological Characteristics ◽  
Food Preservation ◽  
Biochemical Tests ◽  
Antibiotic Susceptibility Test

Aims: The objective of the present study was to investigate the phytochemical constituents and antibacterial activity of ginger extracts against some pathogenic bacteria responsible for Urinary tract infection. Study Design:  A total of 35 samples were collected from patients with UTIs and wound infections. Place and Duration of Study: The study was conducted at 2 hospitals in Baghdad from1/7/2017 to 1/9/2017 Methodology: The urine sample was collected using a sterile container, while a swap from the infected wound was also taken. The classical methods for diagnosis pathogenic bacteria in urine and wound are based on culture on different microbiological media including. Blood agar, nutrient agar, then incubated at 37°C for 24 hrs. The diagnostic procedures consisted of direct microscopy observation, Gram staining, Biochemical tests, Catalase and coagulase tests. Results: Results show that 30.55%, 38.8%, 19.46% and 11.11% isolates gave typical morphological characteristics and biochemical test for Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonae and Staphylococcus aureus respectively. Antibiotic susceptibility test reveals that Escherichia coli isolates were 100% sensitive to gentamicin, tetracycline, streptomycine. Pseudomonas aeruginosa isolates reveal that 100% of them were sensitive to gentamicin, Imipenem, ampicillin and streptomycine. Staphylococcus aureus isolates reveal that 100% of them were sensitive to Gentamycine, tetracycline and streptomycine. Klebsiella pneumonae isolates reveal that 100% of them were sensitive to nitrofurantoin and Imipenem.  Ginger roots extract at high concentration (250,500 mg/ml) have strong antibacterial activity against pathogenic bacteria (Staphylococcus aureus, Escherichia coli and Klebsiella pneumonae). Conclusion: This study has shown that ginger extracts possess medicinal properties, antibacterial activity and that the inhibition of bacterial growth was dose dependent. The results of the present study show that ginger extracts are more effective against all tested bacterial strains. The results of present study have provided the justification for therapeutic potential of ginger and also used as dietary supplement for food preservation.

scholarly journals Isolation of antibiotic Producing Microorganisms from some water bodies within Eastern and Greater Accra Regions of Ghana

2019 ◽  
Author(s):  
Daniel Amiteye ◽  
Nicholas Tete Kwaku Dzifa Dayie ◽  
Stephen Yao Gbedema ◽  
Vivian Etsiapa Boamah ◽  
Francis Adu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Antimicrobial Agents ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Water Bodies ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Biochemical Tests

ABSTRACTBackgroundMost antimicrobial agents used today are isolated and extracted from microbial source. The emergence of antimicrobial resistance and need for better, broad spectrum antimicrobial agent is always in high demand. In this study, a total of 112 aquatic microbial isolates from 14 sample sites of water bodies in Ghana were screened by agar-well diffusion method for the ability of antimicrobial metabolites.ResultsOut of these antibacterial activities, 10 inhibited the growth of at least one test microorganism with zones of growth inhibitions ranged between 2.5±0.5 - 35.5±0.5 mm againstStaphylococcus aureus(ATCC25923),Streptococcus pyogenes(clinical isolates), Escherichia coli(NCTC9002),Pseudomonas aeruginosa(ATCC27853),Neisseria gonorrhoeae(clinical isolate),Klebsiella pneumoniae (Clinical isolate),Bacillus subtilis(NCTC10073) andSalmonella typhi(NCTC 6017). The extracts of the isolates showed minimum inhibitory concentrations of which extract NKSEW3againstEscherichia coli, Klebsiella pneumoniaeandPseudomonas aeruginosawere 25.00, 12.50 and 25.00 mg/mL respectively while that of NKLS6were 12.50, 6.25 and 25.00 mg/mL. The isolates NKSEW3and NKLS6were identified to bePseudomonas speciesusing chromagar and biochemical tests. The GC-MS result for NKLS62 (a fraction obtained from NKLS6) revealed five compounds including; Tridecanal, 17-octadecanal, ethanol, 2-(9-octadecenyloxy)-, (Z), 2-pentadecanone, 6, 10, 14-trimethyl diisooctyl phthalate and 17-octadecanal (C18H34O) as good pharmacological agents.ConclusionMicroorganisms isolated from water bodies in Ghana have the potential of producing antimicrobial agents.Author SummaryIn this study we use the agar well-diffusion to screen the isolates of water and soil samples collected within Greater Accra and Eastern Regions in Ghana against the test organisms.

scholarly journals UJI POTENSI PROBIOTIK Lactobacillus plantarium SECARA IN-VITRO

ALCHEMY ◽  
2013 ◽  
Author(s):  
Anik Maunatin ◽  
Khanifa Khanifa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Growth Efficiency ◽  
Salmonella Typhi ◽  
Probiotic Potential ◽  
Number Of Cells

<p>Probiotic is a product containing non-pathogenic microbes live, which was given to animals or humans to fix the rate of growth, efficiency, and increase conversion ration of animal or human health by affecting positively the balance of the gut microbes and microbial pathogen control in the digestive tract. In order to potentially lactic acid bacteria as probiotic candidates must pass the test selection among others such as probiotic test resistance to acid pH, the bile salts, against pathogenic bacteria. This experimental research methods for descriptive use that aim to provide information about the probiotic potential  of Lactobacillus plantarum was isolated from the small intestine Mojosari duck (<em>Anasplathyrinchos</em>) with the tests in vitro i.e. the resistance of <em>Lactobacillus plantarum</em> on the condition of the acidicpH (2, 3, and 4), bile salt concentration of 0.3% (b/v), inhibition of pathogenic bacteria (<em>Escherichia coli, Staphylococcus aureus</em> and <em>Salmonella typhi</em>) The results showed that on pH 2 the number of cells of bacteria that lives was 4.3. 10<sup>7</sup> CFU/ml, pH 3 was  3.8. 10<sup>9</sup> CFU/ml and pH 4 was 2.7. 10<sup>10</sup> CFU/ml, the resistance of <em>Lactobacillus plantarum</em> on bile salt showed the number of cells of bacteria that lives was 1.2. 10<sup>9</sup> CFU/ml.inhibition of the pathogenic bacteria showed that<em>Lactobacillus plantarum</em>could inhibited strong on Escherichia coli and Staphylococcus aureus with diameter of zone was 12.7 mm and 13.3 mm but not<em>Salmonellatyphi</em> with diameter of zone was 9.3 mm.</p>

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