scholarly journals ACINETOBACTER SPECIES

2018 ◽  
Vol 25 (12) ◽  
pp. 1949-1953
Author(s):  
Aneela Khawaja ◽  
Faiqa Arshad ◽  
Sadaf Aleem
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Acinetobacter Species ◽  
Clinical Specimens ◽  
Sensitivity Pattern ◽  
Acinetobacter Spp

Introduction: The genetic competencies of bacteria and the resistance have been impeding the usefulness of antibiotic therapy. There has been an alarming increase in the infections caused by Acinetobacter spp. especially the multidrug resistance pattern has narrowed the therapeutic ranges. Objectives: To determine the prevalence and antibiotic sensitivity pattern of Acinetobacter spp., among clinical specimens of tertiary care hospital.Study Design: Descriptive study. Place & Duration of Study: Pathology Department, PGMI, from January 2015 to December 2015. Materials & Methods: Total 8465 clinical specimens were inoculated. Acinetobacter spp. was identified and isolated by the preliminary microbiological and biochemical tests. Antimicrobial susceptibility testing was implemented by modified Kirby-Bauer disk diffusion method as per CLSI guidelines (2015). Results: Acinetobacter spp. isolated in 234 (7.29%) clinical specimens among 3208 (37.89%) culture positive isolates. Out of total 234 Acinetobacter spp. isolates 144 (61.54%) were recovered from male patients and 90 (38.46%) from female patients. the frequency of Acinetobacter spp. isolates was seen highest in CSF (23.07%) and lowest in HVS (5.52%) specimens. Maximum samples were recovered from surgical wards 85 (36.32%), while from pediatrics department only 20 (8.54%) samples. Only, 140 (59.82%) isolates were sensitive to tigecycline; while 216 isolates were (92.30%) resistant to salbactam. Conclusion: The progressively increasing threat of Acinetobacter resistant infections can be minimized by judicial use of antibiotics, and implementation of strict infection control policy in health care settings.

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals TO STUDY ANTIBIOTIC SENSITIVITY PATTERN OF NONFERMENTATIVE GRAM NEGATIVE BACILLI FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL, JAIPUR.

2020 ◽  
Vol 4 (5) ◽  
Author(s):  
Kirti Hemwani ◽  
P. S. Nirwan ◽  
Preeti Shrivastava ◽  
Abhiraj Ramchandani
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Polymyxin B ◽  
Hospital Environment ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Pathogenic Potential ◽  
Gram Negative ◽  
Sensitivity Pattern

Background: Nonfermentative gram negative bacilli (NFGNB) frequently considered as commensals or contaminants but the pathogenic potential of nonfermenters has been proved beyond doubt. They are resistant to commonly used antimicrobials. Aim: This study was undertaken to identify the nonfermenters isolated from various clinical samples and to know their Antibiotic sensitivity pattern. Materials and Methods: The present study was carried out on 150 strains of Nonfermenters isolated from 1200 various non repetitive clinical samples received in Department of Microbiology, NIMS Jaipur. Nonfermenters were identified using a standard protocol and their antibiotic susceptibility testing was performed with the help of the modified Bauer disc diffusion method. Results: Out of 150 nonfermenters isolated, Pseudomonas aeruginosa was the most common isolate 134 (89.33%) followed by Acinetobacter baumannii 16 (10.67%). Among all clinical samples Pus and Wound Discharge yield maximum isolates of NFGNB i.e. 54 (36%) % followed by sputum (39.0%). Most sensitive drug against NFGNB was Polymyxin-B (100%) followed by Imipenem (86 %) and Amikacin (71.33 %). Conclusion: Nonfermenters have a great potential to survive in a hospital environment so implementation of antibiotic stewardship programs and strict infection control practices will be required to prevent or slow down their emergence and spread. Keywords:  Nonfermenters,  Polymyxin-B, Pseudomonas, Acinetobacter.

scholarly journals Bacterial Etiology of Wound Exudates in Tertiary Care Cancer Patients and Antibiogram of the Isolates

2020 ◽  
Vol 13 ◽  
pp. 117863372095207
Author(s):  
Kiran Duwadi ◽  
Sujan Khadka ◽  
Sanjib Adhikari ◽  
Sanjeep Sapkota ◽  
Pabitra Shrestha
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Methicillin Resistance ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Biochemical Tests ◽  
Cross Sectional

Introduction: Patients with malignancies frequently develop infections as a result of surgical procedures and fungating wounds leading to pus formation. This cross-sectional study was conducted to explore the bacteriological spectra of infections of various cancer sites and their antibiotic sensitivity patterns among the patients visiting minor operation theatre (OT) of B.P. Koirala Memorial Cancer Hospital (BPKMCH), Chitwan, Nepal. Methods: Over a period of 3 months from September to November 2018, a total of 183 wound exudates and pus samples were collected and analyzed by standard microbiological procedures. Isolates were identified based on the colony characters, Gram staining and an array of biochemical tests. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion technique according to criteria set by CLSI, 2016. Methicillin resistance in Staphylococcus aureus was tested with the help of cefoxitin using disc diffusion method. Results: Out of the 183 samples, 149 (81.4%) were culture positive. Among 13 different isolates identified, S. aureus (43.0%) was predominant followed by E. coli (14.0%). Higher incidence of bacteria was seen among the males (52.3%), in the age group 51 to 60 years (26.8%) and among the patients undergoing surgical intervention to deal with cancer (34.2%). The prevalence of wound infection was significantly affected by gender, age, and treatment regimen ( P < .01). Out of the total 68 S. aureus isolates, 38 (44.1%) were deemed as Methicillin-resistant Staphylococcus aureus (MRSA). Among the 158 isolates, 85 (53.8%) were multi-drug resistant (MDR). Cefepime was the most effective antibiotic for Gram positive isolates whereas both imipenem and meropenem were found to be equally more effective for Gram negative isolates. Conclusion: This study suggests that patients with malignancies harbor pathogenic bacteria; therefore, prudent use of antibiotics is essential to prevent the emergence of MDR pathogens.

scholarly journals Beta-Lactamases Production in Multi-drug Resistant Acinetobacter species Isolated from Different Clinical Specimens

2019 ◽  
Vol 6 ◽  
pp. 44-50
Author(s):  
Mary Neupane ◽  
Sudeep K.C. ◽  
Subash Kumar Thakur ◽  
Om Prakash Panta ◽  
Dev Raj Joshi ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Acinetobacter Calcoaceticus ◽  
Clinical Samples ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Acinetobacter Species ◽  
Antibiotic Susceptibility Pattern ◽  
Beta Lactamases ◽  
Clinical Specimens ◽  
Acinetobacter Spp

Objectives: To determine the prevalence of Acinetobacter spp. from different clinical specimens and detect different types of β-lactamase enzymes. Methods: Different clinical samples were collected and 125 Acinetobacter spp. were isolated.  Various biochemical tests were carried out to speciate the Acinetobacter spp. The antibiotic susceptibility pattern and β-lactamase enzymes like Extended spectrum β-lactamase (ESBL), Metallo β-lactamase (MBL) and AmpC β-lactamase were determined. Results: Of the total 125 isolates, the most predominant species was Acinetobacter calcoaceticus-A. baumannii (Acb) complex (80%). Highest rate of isolation of Acinetobacter species were from in-patients (neonates’ blood sample).  Among all, 44.8% isolates were found to be MDR with the majority being resistant to aminoglycosides, carbapenems and fluoroquinolones but not to colistin. ESBL, MBL and AmpC beta-lactamase was detected in 43.2%, 15.2% and 1.6% of the isolates respectively. Conclusion: Acinetobacter calcoaceticus-A. baumannii complex should be considered for detection in hospitalized patients. The analysis of antibiotic susceptibility pattern and β-lactamases would be helpful to establish network surveillance in order to maintain and control the spread of these resistant strains.

scholarly journals Ecology of Bloodstream Infections and Temporal Trends of Their Antibiograms with Respect to Source and Duration of Incubation: A 5-Year Retrospective Observational Analysis

2020 ◽  
Vol 12 (01) ◽  
pp. 56-67
Author(s):  
Amit Banik ◽  
Valarie W. Lyngdoh ◽  
Elantamilan Durairaj ◽  
Anil C. Phukan ◽  
Raghavendra Kotal
Keyword(s):  
Tertiary Care ◽  
Temporal Trends ◽  
Bloodstream Infections ◽  
Diffusion Method ◽  
Blood Collection ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Sensitivity Testing ◽  
Disk Diffusion Method ◽  
Significant Difference

Abstract Purpose Blood is one of the most important connective tissues of human body. Bloodstream infection can range from inapparent bacteremia till fulminant septic shock with high mortality. Presence of microbes in blood whether continuously, intermittently, or transiently is a grave risk to every organ of body. Culture of blood is a vital tool to diagnose such infections. Drug susceptibility patterns help in rationalizing therapy. Objective The aim of the study is to perform bacteriological analysis and assess drug sensitivity patterns of blood culture isolates and compare in light of other associated variables. Design Retrospective observational study was conducted from January 2009 to December 2013 at a tertiary care hospital at Shillong, India. Blood samples were collected with aseptic guidelines and cultured for 7 days. Growths were identified by standard biochemical tests and subjected to sensitivity testing according to Modified Kirby Bauer disk diffusion method. Data for source of blood collection and duration of incubation were noted and compared. Results A total of 658 (11.2%) pathogens were isolated from 5,867 bacteremia-suspected patient blood specimens. Contamination was observed at the rate of 1.21%. Gram-negative organisms were the predominant pathogens recovered, Klebsiella pneumoniae being the most common. No significant difference was observed between the number of organisms isolated within or beyond 48 hours. Acinetobacter baumannii and K. pneumoniae have significantly higher chances (p < 0.05) of isolation from central line catheters compared with peripheral venipuncture. Conclusion Successful treatment of sepsis depends on early diagnosis and proper antimicrobial therapy. Local knowledge of bacteriological profile and antimicrobial sensitivity patterns helps rationalize empiric treatment strategies.

scholarly journals Bloodstream infections and trends of antimicrobial sensitivity patterns at Port Blair

2018 ◽  
Vol 10 (03) ◽  
pp. 332-337 ◽  
Author(s):  
Amit Banik ◽  
Sanjeev H. Bhat ◽  
Abhay Kumar ◽  
Agnijeet Palit ◽  
Kandregula Snehaa
Keyword(s):  
Tertiary Care ◽  
Bloodstream Infections ◽  
The Body ◽  
Diffusion Method ◽  
Blood Collection ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Sensitivity Testing ◽  
Disk Diffusion Method ◽  
Antimicrobial Sensitivity

ABSTRACT PURPOSE: Bloodstream infection can range from inapparent bacteremia until fulminant septic shock with high mortality. Microorganisms present in circulating blood whether continuously, intermittently, or transiently are a threat to every organ in the body. Culture of blood is a vital tool to diagnose such infections. Drug susceptibility patterns help in rationalizing therapy. OBJECTIVE: The objective of this study was to perform bacteriological analysis and assess drug sensitivity patterns of isolates from blood stream infections. DESIGN: Retrospective observational study was conducted from May 2015 to February 2017 at a tertiary care hospital, Port Blair, India. Blood samples were collected with aseptic guidelines and cultured for 7 days. Growths were identified using standard biochemical tests and subjected to sensitivity testing according to Modified Kirby–Bauer’s disk diffusion method. Data for the source of blood collection and duration of incubation were noted and compared. RESULTS: A total of 270 (14.24%) pathogens were isolated from 1895 bacteremia suspect patient blood specimens. Contamination was observed at a rate of 1.63%. Gram-positive cocci (60.37%) were predominant organisms recovered followed by Gram-negative Bacilli (36.29%) and Yeasts (3.33%). Staphylococcus aureus, CoNS, and Acinetobacter spp. were the primary pathogens isolated. Aminoglycosides, carbapenems, and glycopeptides were the most effective drugs for treating bacteremia. CONCLUSIONS: Successful treatment of sepsis depends on early diagnosis and proper antimicrobial therapy. Local knowledge of bacteriological profile and antimicrobial sensitivity patterns helps rationalize empiric treatment strategies.

scholarly journals A study of microbial gamut and antibiograms in chronic suppurative otitis media

2018 ◽  
Vol 5 (1) ◽  
pp. 110
Author(s):  
Raj Tajamul Hussain ◽  
Sanam Altaf ◽  
Owais Makhdoomi ◽  
Mariya Ali
Keyword(s):  
Otitis Media ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Chronic Suppurative Otitis Media ◽  
Fungal Culture ◽  
Care Hospital ◽  
Biochemical Tests ◽  
Suppurative Otitis Media ◽  
Gram Staining ◽  
Sensitivity Pattern

<p class="abstract"><strong>Background:</strong> The aim of the study was to discern the patterns in microbial diversity and the resistogram among the patients suffering from CSOM.</p><p class="abstract"><strong>Methods:</strong> A total number of 100 ear swabs were investigated for the present study. Their gram staining, direct microscopy with KOH, culture sensitivity, and biochemical tests were carried out to identify the organisms and to know their sensitivity pattern. All the swabs were collected from clinically diagnosed cases of chronic suppurative otitis media visiting otolaryngology outpatient department of tertiary care hospital. The study period was one year, from January 2016 to December 2017.  </p><p class="abstract"><strong>Results:</strong> Out of total 100 cases, 90 were culture<strong> </strong>positives, 6 showed no growth and 4 were<strong> </strong>skin contaminants (mirococci). Out of 90 culture positives, fungal culture was positive in 5 (5.5%) while combined bacteria and fungi obtained in 18 (20%) cases and only bacteria in 67 (74.4%) cases. Among the aerobic bacterial isolates, Pseudomonas aeruginosa was the most common bacteria isolated from the bacterial culture (n=36; 34.95%) followed by <em>Staphylococcus aureus </em>(n=28; 27.18%) and <em>Proteus</em> (n=13; 12.62%). Among the fungal isolates,<strong> </strong><em>Aspergillus niger </em>was predominant followed<strong> </strong>by Candida <em>albicans </em>&amp; <em>Aspergillus flavus.</em> Amikacin and imipenem were found to be the most effective antibiotics with low resistance rates.</p><p><strong>Conclusions:</strong> The present study gave an insight into the bacteriological profile of the cases of CSOM and their antibiotic sensitivity patterns. This in turn will ensure rational and judicious use of antibiotics and thus prevent emergence of resistant bugs and also the complications associated with CSOM.</p>

Antimicrobial susceptibility of clinical Staphylococcus aureus isolated from patients with an ear infection in Misan

2018 ◽  
Vol 9 (3) ◽  
pp. 1016
Author(s):  
Munaf Aal-Aaboda ◽  
Mohammed R. Al-Notazy
Keyword(s):  
Antibiotic Susceptibility ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Antibiotics Resistance ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Ear Infection ◽  
Mannitol Salt Agar ◽  
Causative Agents ◽  
High Level

The present study was targeted to examine the prevalence of multi-drug resistant Staphylococcusaureus,which has been carried out in Misan, Iraq at a local hospital from February 2016 to January 2017.A hundred and eighty ear swabs have been obtained from patients with ear infections with or without discharges. Culturing and identifying the causative agents, as well as the antibiotic sensitivity profile, have been done on the specimens. Swabs were collected under sterile conditions and instantly transferred to the laboratory sealed in brain heart broth tubes. The initial isolation was done on selective media to S. aureus(mannitol salt agar) at a temperature of 37°C for 24 - 48 hours and then the biochemical tests and identification were done in accordance with the standard monotonous techniques. Antibiotic susceptibility tests were done by the disk diffusion method. A hundred and forty-four isolates diagnosed with Staphylococcus aureusand eighteen isolates as other bacteria. S.aureusisolates tested for antibiotic susceptibility showed high resistance to ampicillin, carbenicillin and amoxicillin, mild resistance to co-trimoxazole and were susceptible to norfloxacin, rifampicin, and ciprofloxacin.Additionally, S.aureusisolates showed multiple antibiotic resistance (MAR). The MAR index of the isolates found to range between 0.35 and 0.7. In conclusion, an ear infection is mostly caused by Staphylococcus aureusand most of these isolates showed a high level of antibiotics resistance, which eventually may lead to too many health-related consequences in Misan, Southern Iraq and expose the needs for further studies to lessen the resistance to antibiotics.

scholarly journals Prevalence of Methicillin-resistant Staphylococcus Aureus (MRSA) in a Tertiary Care Hospital in Northern India

2010 ◽  
Vol 2 (02) ◽  
pp. 078-081 ◽  
Author(s):  
Shilpa Arora ◽  
Pushpa Devi ◽  
Usha Arora ◽  
Bimla Devi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Care Hospital ◽  
Methicillin Resistant ◽  
Sensitivity Pattern

ABSTRACT Aim: The emergence of Methicillin-resistant Staphylococcus aureus (MRSA) has posed a serious therapeutic challenge. We report the prevalence and antibiotic susceptibility pattern of MRSA in the hospitals attached to GMC, Amritsar, Punjab. Materials and Methods: The study comprised of 250 coagulase-positive staphylococci (COPS) isolated from a total of 6743 clinical specimens (like pus, blood, urine, high vaginal swab, sputum, etc.) of patients admitted in hospitals attached to Government Medical College, Amritsar from January 2008−February 2009. Routine antibiotic susceptibility testing was performed and interpreted as per standard guidelines. Methicillin resistance was detected using oxacillin and cefoxitin disc diffusion method, oxacillin screen agar method, and minimum inhibitory concentration using broth macrodilution method. Results: A total of 115 (46%) strains were found to be methicillin resistant. Multidrug resistance was observed in 73% MRSA strains. However, no strain was resistant to vancomycin. Conclusion: Regular surveillance of hospital-associated infection and monitoring of antibiotic sensitivity pattern is required to reduce MRSA prevalence.

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