DETERMINATION OF ANTIFUNGAL ACTIVITY OF HERBAL OINTMENT PREPARED FROM LEAF EXTRACT OF Cassia fistula ON LABORATORY SPECIMEN OF Candida albicans

2020 ◽  
Vol 10 (3) ◽  
pp. p99100
Author(s):  
Fernando KAB ◽  
Waliwita WALC
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Cassia Fistula ◽  
Laboratory Specimen

scholarly journals Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

2014 ◽  
Vol 43 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Cíntia Lima Gouveia ◽  
Isabelle Cristine Melo Freire ◽  
Maria Luísa de Alencar e Silva Leite ◽  
Rebeca Dantas Alves Figueiredo ◽  
Leopoldina de Fátima Dantas de Almeida ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Sodium Bicarbonate ◽  
Fungal Growth ◽  
Antifungal Effect ◽  
Dental Prostheses ◽  
Diffusion Technique ◽  
Zones Of Inhibition ◽  
And Control

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

A rapid Candida albicans hyphal-form growth inhibition assay: determination of myelomonocytic-mediated antifungal activity

Mycoses ◽  
2009 ◽  
Vol 34 (3-4) ◽  
pp. 119-123 ◽  
Author(s):  
Lucia Scaringi ◽  
Elisabetta Blasi ◽  
Paola Cornacchione ◽  
Carla Bietta ◽  
F. Bistoni
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Inhibition Assay ◽  
Growth Inhibition Assay

scholarly journals IN VITRO ANTIFUNGAL ACTIVITY OF TIMOL AND CARVACROL ON CANDIDA SPECIES

2019 ◽  
Vol 4 ◽  
Author(s):  
Luciana Thaís Rangel Souza ◽  
Cecília Correia Costa ◽  
Mateus Cardoso Oliveira ◽  
Isabel Celeste Caires Pereira Gusmão
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Candida Krusei ◽  
Minimum Fungicidal Concentration ◽  
Sabouraud Agar ◽  
In Vitro Antifungal Activity

Aim: to evaluate the in vitro action of thymol and carvacrol against the yeasts of Candida albicans ATCC10231 and Candida krusei ATCC34135. Method: A laboratory study was performed to evaluate antifungal activity. The characterization of the Minimal Inhibitory Concentration (MIC) of the thymol essential oil was carried out using the technique where the microdilution is performed, in which a plate containing 96 wells is used. The determination of the Minimum Fungicidal Concentration (MFC) was performed by dripping 10 μL of each of the concentrations evaluated on Sabouraud agar plates. Results: The MIC of thymol and carvacrol for C. albicans was 40 μg/mL and for Candida krusei it did not present antifungal activity. While the MIC of nystatin was 0.03mg for both species with thymol and carvacrol. Conclusion: Thymol presented satisfactory antifungal activity against the pathogens studied, but carvacrol did not present antifungal activity.

scholarly journals FORMULASI DAN UJI EFEKTIVITAS ANTIFUNGI KRIM EKSTRAK ETANOL DAUN SALAM (Syzygium polianthum (Wight) Walp.) TERHADAP JAMUR Candida albicans

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 841
Author(s):  
Sheren N. Lolowang ◽  
Paulina V.Y. Yamlean ◽  
Karlah L.R. Mansauda
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Physical Stability ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Statistical Test ◽  
Adhesion Test ◽  
Physical Evaluation ◽  
Dispersion Test

ABSTRACT Bay leaf (Syzygium polianthum (Wight) Walp.) are used as natural medicinal ingredients because contain compounds such as flavonoid, saponin and tannin that can inhibit the growth of fungal. The purpose of this study was to test the antifungal effectiveness of bay leaf ethanol extract cream and evaluate the preparation of physical stability. The research that was carried out was laboratory experimental. Bay leaf extract was obtained by maceration using 96% ethanol. The cream preparation was made with variations in the concentration of Bay leaf ethanol extract formula 1%; 3%; 6% and 9%. The method used to test the antifungal effectiveness is the well method. The result of the antifungal test showed an average diameter value for formula I (1%) 6.67 mm; formula II (3%) 9.5 mm; formula III (6%) 10.83 mm and formula IV (9%) 15 mm. Statistical test of ethanol extract cream of Bay leaf produced the largest inhibiton zone of 15 mm at a concentration of 9%. Physical evaluation showed that the cream preparations met organoleptic requirements, homogeneity, pH 4.83, dispersion  test 5.30 cm, adhesion test 7.01 seconds. It can be concluded that the ethanol extract of bay leaf can be formulated into a cream preparation that is physically stable and has strong antifungal activity.  Keywords: Bay Leaf, Antifungal Cream, Candida albicans  ABSTRAK Daun Salam (Syzygium polianthum (Wight) Walp.) dimanfaatkan sebagai bahan obat alami karena memiliki kandungan senyawa flavonoid, saponin dan tannin yang dapat menghambat pertumbuhan jamur. Penelitian ini bertujuan menguji efektivitas antijamur sediaan krim ekstrak etanol daun salam dan mengevaluasi kestabilan fisik sediaan. Penelitian yang dilakukan bersifat eksperimental laboratorium. Ekstrak daun Salam diperoleh dengan cara maserasi menggunakan etanol 96%. Sediaan krim dibuat dengan variasi konsentrasi formula ekstrak daun Salam  1%; 3%; 6% dan 9%. Metode yang digunakan untuk uji efektivitas antijamur yaitu metode sumuran. Hasil pengujian antijamur menunjukkan nilai diameter rata-rata pada formula I (1%) 6.67 mm; formula II (3%) 9.5 mm; formula III (6%) 10.83 mm dan formula IV (9%) 15 mm. Uji statistika krim ekstrak etanol daun salam menghasilkan zona hambat terbesar yaitu 15 mm pada formula IV konsentrasi 9%. Evaluasi fisik menunjukkan bahwa sediaan krim memenuhi persyaratan organoleptik, homogenitas, pH 4.83, daya sebar 5.30 cm, dan daya lekat 7.01 detik. Dapat disimpulkan bahwa ekstrak etanol daun salam dapat diformulasikan menjadi sediaan krim yang stabil secara fisik dan memiliki aktivitas antijamur yang kuat. Kata kunci: Daun Salam, Krim Antijamur, Candida albicans

scholarly journals Antifungal Activity of Topical Formulation Containing Artemisia Herba Alba Asso Essential Oil

2020 ◽  
Vol 2 (4) ◽  
pp. 69-73
Author(s):  
Asma Boukhennoufa ◽  
Yamina Maizi ◽  
Aicha Tir Touil Meddah ◽  
Boumediene Meddah
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Ph Value ◽  
Topical Formulation ◽  
Synthetic Drugs ◽  
Zones Of Inhibition ◽  
Yeasts And Molds ◽  
Indirect Source

Given the increase in antibiotic resistance phenomena and the undesirable effects of synthetic drugs. Medicinal plants are used as a direct or indirect source of the active ingredients. Our research constitutes a development of essential oils from Artemisia herba alba Asso cultivated in the Mascara region. A hydrophobic ointment has been formulated based on this essential oil and tested against five strains of Candida albicans. Several physicochemical and microbiological tests were used to verify the quality and the toxicity of the product. Then, the determination of the antifungal activity of this preparation was assessed against five strains of Candida albicans (S1, S2, S3, S4 and S5) by the disk-diffusion agar method. The results revealed that this preparation was devoid of total aerobic germs, yeasts and molds.  However, the pH value was found equal to 5.98.  In addition, the irritation primary index was marked less than 0.5. The ointment was powerful against S1, S3, S4 and S5 strains with inhibition diameters ranging from 16± 4 mm to 23 ± 2 mm. The Nystatin ointment was observed active against strains S2, S4 and S5 with diameters of the zones of inhibition; 21 ± 2 mm, 21± 1 mm and 20 ± 3 mm respectively. The ointment formulated with essential oil from Artemisia herba alba asso has proven useful against candidiasis caused by Candida albicans species.

scholarly journals Anti-fungal activity of Carica papaya leaf extract against candida albicans and its synergy with fluconazole: an in-vitro study

2020 ◽  
Vol 10 (1) ◽  
pp. 101
Author(s):  
Nilofer Noorie M. ◽  
Chenthamarai G.
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Synergistic Activity ◽  
Disc Diffusion ◽  
Disc Diffusion Assay ◽  
Diffusion Assay

Background: In this study objectives were to evaluate the antifungal activity in increasing concentrations of ethanolic extract and aqueous extract of Carica papaya against Candida albicans and to assess the synergistic activity of ethanolic extract of Caricia papaya with flucanazole as a potential antifungal.Methods: The aim of the study was to evaluate the antifungal activity in increasing concentrations, 500 µl/ml, 750 µl/ml and 1000 µl/ml of ethanolic extract and aqueous extract of caricia papaya against Candida albicans and the synergistic activity with Fluconazole was assessed by observing the zone of inhibition in agar disc diffusion assay and by observing the turbidity in minimum inhibitory concentration (MIC) assay.Results: It was observed that ethanolic extract of Carica papaya leaf showed significant antifungal activity in higher concentration of 1000µg/ml with zone diameter of 11.97±0.15 mm in disc diffusion assay and MIC of 350 µg/ml. The ethanolic extract of Carica papaya leaf with fluconazole showed synergistic activity with zone diameter of 13.6±0.45 mm in disc diffusion assay and MIC was 125µg/ml, whereas the standard drug Fluconazole’s zone of inhibiton was 12.83±0.9 in disc diffusion and MIC was 500 µg/ml.Conclusions: From this study, we can safely conclude that the Carica papaya leaf extract has a significant antifungal property and exhibit synergistic effect when used with fluconazole.

scholarly journals Antifungal activity of plant-based tinctures on Candida

RSBO ◽  
2013 ◽  
Vol 9 (1) ◽  
pp. 25-30
Author(s):  
Andreia Medeiros Rodrigues Cardoso ◽  
Yuri Wanderley Cavalcanti ◽  
Leopoldina de Fátima Dantas de Almeida ◽  
Ana Luíza Alves de Lima Pérez ◽  
Wilton Wilney Nascimento Padilha
Keyword(s):  
Candida Albicans ◽  
Statistical Analysis ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Inhibitory Concentration ◽  
Fungal Growth ◽  
Initial Concentration ◽  
Malva Sylvestris ◽  
Fungal Inhibition

To evaluate through determination of minimum inhibitory concentration (MIC) the antifungal activity of Salvia officinalis (sage), Anacardium occidentale (cashew) and Malva sylvestris (mallow) tinctures on Candida albicans (ATCC 40227), C. tropicalis (ATCC 13803) and C. krusei (ATCC 40147). Material and methods: In 96-well microplates, 100 µl of Sabouraud-Dextrose broth doubly concentrated, 100 µl of the tested tinctures and 10 µl of fungal inoculums (1.5 x 106organisms/ml) were inserted. The products were diluted from initial concentration of 100 mg/ml until 0.78 mg/ml. MIC corresponded to the lowest dilution at which there was no visible fungal growth. Nystatin (100,000 UI/ml) was used as control. Statistical analysis was performed by Kruskal-Wallis and Dunn tests (p < 0.05). Results: S. officinalis tincture did not inhibit the growth of C. albicans and C. tropicalis; MIC was 100 mg/ml for C. krusei. For A. occidentale, MIC was 100 mg/ml for C. albicans and C. krusei, and for C. tropicalis, there was no fungal inhibition. M. sylvestris tincture presented MIC at 25 mg/ml for C. krusei and 100 mg/ml for C. albicans and C. tropicalis. The best antifungal activity was showed by M. sylvestris tincture (p < 0.05). Conclusion: M. sylvestris tincture exhibited antifungal activity against all the tested strains at lower concentrations. S. officinalis tincture inhibited the action of C. krusei and A. occidentale tincture showed activity against C. albicans and C. tropicalis.

scholarly journals CABBAGELEAF EXTRACT(BRASSICA OLERACEA VAR. CAPITATA ALBA) AS A HERBAL MEDICINE FOR LEUCORRHEA

2017 ◽  
Vol 10 (14) ◽  
pp. 39
Author(s):  
Ami Tjitraresmi ◽  
Sri Agung Fitri Kusuma ◽  
Dewi Rusmiati
Keyword(s):  
Candida Albicans ◽  
Comparative Analysis ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Herbal Medicine ◽  
Contact Time ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Diffusion Method ◽  
Leaf Extracts

Objective:This study was aimed to examine antifungal activity of cabbage leaf extracts against Candida albicans, determined the minimum inhibitory concentration (MIC), analyzed the comparative value of the extracts with ketoconazole and studied the fastest contact time to eliminate Candida albicans. Methods: The extraction of fresh cabbage leaf was done using the maceration method. The antifungal activity test and its comparative analysis against ketokonazole were assessed using the agar diffusion method. The extracts were tested for determining minimum inhibitory concentration value (MIC) using solid medium. While the fastest contact time test was performed using turbidimetric method.Results: Based on its inhibitory diameter, cabbage leaf extracts gave potent antifungal activity against Candida albicans. The MIC concentrations of testing extracts were various ranged from 1.50 to 1.75%w/v. In a comparative analysis of the extracts with ketoconazole, showed that ketoconazole gave greater antifungal activity than the extract at the same concentration. Cabbage leaf extract with concentration 2.5% gave the fastest contact time (2.5 minutes) for eliminating Candida albicans, while Cabbage leaf extract with concentration 0.4% gave the longest contact time (15 minutes) for eliminating Candida albicans.Conclusion:Cabbage leaf extract has a potential antifungal activity against Candida albicans, and prospective to be developed as a topical herbal medicine for treating candidiasis.  

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