scholarly journals Dynamics of Matrix Metalloproteinases in Local Cold Injury

2019 ◽  
Vol 4 (5) ◽  
pp. 79-83 ◽  
Author(s):  
M. I. Mikhailichenko ◽  
K. G. Shapovalov ◽  
V. A. Mudrov ◽  
O. S. Gruzdeva
Keyword(s):  
Endothelial Dysfunction ◽  
Matrix Metalloproteinases ◽  
Specific Weight ◽  
Cold Injury ◽  
Biologically Active ◽  
Control Group ◽  
Control Parameters ◽  
Dual Nature ◽  
Leading Role ◽  
Local Cold Injury

The relevance of cold injury is due to its high specific weight in the structure of injuries, complexities of complex therapy, and unsatisfactory results of treatment. It is known that in the pathogenesis of local cold injury, the leading role belongs to endothelial dysfunction, which secretes a huge amount of biologically active substances, including matrix metalloproteinases.Aim: to determine the dynamics of the content of matrix metalloproteinases of the second subfamily (MMP-2, MMP-9) in the serum of patients with local cold injury.Materials and methods. The study included 80 patients (60 men, 20 women) with frostbite of the III–IV degree of the foot to the level of the lower third of the leg in the late reactive period and the period of granulation and epithelization.Results. In the late reactive period in patients with cryopreservation, the level of MMP-2 was 3.4 times higher relative to the control (p = 0.011), on day 30 MMP-2 values did not differ from the control parameters (p = 0.103). The level of collagenase B (MMP-9) in patients with local cold injury on day 5 was 14.5 times higher than the control parameters (p = 0.002), on day 30 – 12.5 times higher compared to the control group (p = 0.000094).Conclusion. During the analysis of the data obtained, we can think about the dual nature of collagenases during the processes of inflammation. In a situation of distress, collagenases prevent endothelial dysfunction by eliminating the cellular structures of the endothelium to ensure adequate metabolism in tissues. Their importance as markers of failure of adequate flow of reparative processes is not excluded.

scholarly journals Dynamics of the Level of Myeloperoxidase and Serum Calprotectin in Local Cold Injury

2020 ◽  
Vol 5 (3) ◽  
pp. 24-28
Author(s):  
M. I. Mikhailichenko ◽  
K. G. Shapovalov ◽  
V. A. Mudrov ◽  
O. S. Gruzdeva
Keyword(s):  
Endothelial Dysfunction ◽  
Blood Serum ◽  
Human Body ◽  
Cold Injury ◽  
Control Group ◽  
Multiplex Analysis ◽  
General Cooling ◽  
The Moment ◽  
Local Cold Injury

Relevance. Cold injury is defined as a complex of pathophysiological and pathomorphological changes arising as a result of local or general cooling of the human body. Endothelial dysfunction provokes a powerful cascade of cellular interactions with expressed excretory activity, which ultimately leads to a pronounced remodeling of microcirculation and a protracted process of inflammation in the focus of alterations. Aim of the study. To establish the dynamics of the level of myeloperoxidase and calprotectin in the serum of patients with local cold injury. Materials and methods. The study included 80 patients with III–IV degree lesions in the late reactive period and the period of granulation and epithelialization. The average age of patients was 38 ± 8 years. The myeloperoxidase level was measured on the 5th and 30th days from the moment of cryopreservation using multiplex analysis of blood serum. Results. The level of myeloperoxidase and serum calprotectin increases. In late reactive period (day 5), MPO level in patients with frostbite is 7.25 times higher in comparison with control values, in the period of granulation and epithelialization (day 30), it remains elevated, but only 3.63 times higher than in the control group. In the late reactive period, the level of calprotectin in the blood serum of patients with local cold injury was 4.6 times higher in comparison with control values, and on the 30th day of cryopreservation, the value of calprotectin was 4.5 times higher than in the control group. Changes in the level of myeloperoxidase and serum calprotectin reflect the flow of destructive and reparative mechanisms in tissues during local cold trauma and can be used in predicting an unfavorable prolonged course of the wound process.

scholarly journals Pathogenetic significance of endothelial dysfunction in formation of vascular wall hypertonia in local cold trauma

2020 ◽  
pp. 54-61
Author(s):  
М.И. Михайличенко ◽  
К.Г. Шаповалов ◽  
В.А. Мудров ◽  
С.А. Фигурский ◽  
Р.С. Емельянов
Keyword(s):  
Control Level ◽  
Lower Extremities ◽  
Fold Increase ◽  
Vascular Wall ◽  
Cold Injury ◽  
Circulating Endothelial Cells ◽  
Control Group ◽  
Blood Levels ◽  
Control Value ◽  
Local Cold Injury

Актуальность. В структуре общего травматизма местная холодовая травма имеет большой удельный вес. Выявление новых маркеров криоповреждения способствует более ранней диагностике глубины поражений тканей при обморожении, созданию новых подходов к хирургическому лечению и ускоренной реабилитации пострадавших. Цель исследования - изучение в динамике содержания оксида азота, асимметричного диметиларгинина и циркулирующих эндотелиальных клеток в сыворотке крови у пациентов с местной холодовой травмой. Методика. В исследование включено 80 пациентов с обморожениями нижних конечностей III-IV степени в позднем реактивном периоде и периоде гранулирования и эпителизации. Использован мультиплексный анализ сыворотки крови набором реагентов фирмы Biomedical (США), методом Hladovec (1978) и методом П.П. Голикова (2004) соответвенно. Результаты. У пациентов с криотравмой во всех исследуемых группах установлено снижение уровня оксида азота относительно контроля. У пострадавших с местной холодовой травмой на 5-е сут отмечено значительное снижение в крови уровня асимметричного диметиларгинина. на 30-е сут с момента криоповреждения уровень асимметричного диметиларгинина не отличался от показателей контрольной группы. При обморожении дистальных сегментов стоп концентрация асимметричного диметиларгинина в крови не менялась относительно контроля. У пациентов с тяжелыми обморожениями нижних конечностей уровень асимметричного диметиларгинина в крови снижался в 3,6 раза. У пациентов с местной холодовой травмой на 5-е сут установлено увеличение в крови уровня циркулирующих эндотелиальных клеток в 5,2 раза, на 30-е сут с момента криоповреждения уровень циркулирующих эндотелиальных клеток снижался, но, по-прежнему, превышал контрольные значения. У пострадавших с обморожением дистальных сегментов стоп содержание в крови циркулирующих эндотелиальных клеток увеличилось в 2,7 раза, с поражением проксимальных сегментов нижних конечностей - в 6,7 раза относительно здоровых добровольцев. У пациентов с наиболее тяжелыми обморожениями нижних конечностей уровень циркулирующих эндотелиальных клеток в крови повышался в 9 раз. The relevance of local cold injury remains high. In the structure of general injury prevalence, proportion of freeze burns is great. Identification of new markers of cold injury will help earlier determination of tissue lesion depth, development of new approaches in the surgical treatment of deep freeze burns of extremities, and promote rehabilitation of patients. The aim of this study was to elucidate the dynamics of the serum content of nitric oxide (NO), asymmetric dimethylarginine (ADMA), and circulating endothelial cells (CEC) in patients with local cold injury. Methods. The study included 80 patients with III-IV degree frostbite of the lower extremities in the late reactive period and the period of granulation and epithelization. Multiplex analyses of blood serum were performed with Biomedical (USA) reagent kits, according to methods by Hladovec (1978) and Golikov (2004). Results. In all groups of patients with frostbite, NO level was decreased compared to the control. Patients with local cold trauma displayed significant decreases in blood levels of ADMA on day 5; on day 30 after the cold trauma, the ADMA level did not differ from the control group. In patients with frostbite of the distal foot segments, the blood concentration of ADMA did not change from the control level. In patients with the most severe frostbite of the lower extremities, serum ADMA decreased 3.6 times. Patients with local cold trauma had a 5.2-fold increase in the blood level of CEC on day 5. On day 30 after the freeze burn, the amount of CEC was decreased but still remained above the control value. In patients with frostbite of the distal foot segments, the content of CEC increased 2.7 times compared to the control. In patients with lesions of more proximal segments of the lower extremities, the CEC index was increased 6.7 times compared to healthy volunteers. In patients with the most severe frostbite of the lower extremities, the blood content of CEC was 9 times increased.

scholarly journals Microcirculatory disturbances in the pathogenesis of local cold injuries

2019 ◽  
Vol 18 (2) ◽  
pp. 4-11
Author(s):  
M. I. Mikhailichenko ◽  
K. G. Shapovalov
Keyword(s):  
Nitric Oxide ◽  
Endothelial Dysfunction ◽  
Platelet Adhesion ◽  
Intercellular Adhesion ◽  
Cold Injury ◽  
Endocrine Activity ◽  
Microcirculatory Bed ◽  
Microcirculatory Disturbances ◽  
Microcirculatory Disorders ◽  
Local Cold Injury

The review is devoted to microcirculatory disorders in local cold injury and simultaneous endothelial dysfunction. The features of endocrine activity of endothelium, cytokine activity, expression of the main molecules of intercellular adhesion and the phenomenon of lymphocytic-platelet adhesion, metabolism of nitric oxide, the state of the microcirculatory bed of the victims in different periods of injury were described.

The role of matrix metalloproteinases and their inhibitors in patients with local cold injury

2020 ◽  
pp. 72-76
Author(s):  
M.I. Mikhaylichenko ◽  
K.G. Shapovalov ◽  
V.A. Mudrov ◽  
O.S. Gruzdeva
Keyword(s):  
Matrix Metalloproteinases ◽  
Cold Injury ◽  
Local Cold Injury

The state of lipid peroxidation and antioxidant protection systems in patients with different of local cold injury of the extremities

2020 ◽  
pp. 5-10
Author(s):  
O.S. Gruzdeva ◽  
K.G. Shapovalov ◽  
M.I. Mikhailichenko
Keyword(s):  
Lipid Peroxidation ◽  
The State ◽  
Cold Injury ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Protection Systems ◽  
Early Late ◽  
Free Radical Lipid Oxidation ◽  
Local Cold Injury

The authors studied the state of lipid peroxidation systems and antioxidant protection in of the extremities in different periods. It was revealed that in patients with frostbite of the radical oxidation and the level of antioxidant protection depended on periods of cold 20 people were in the control group, 30 patients with local cold trauma in the early reactive late reactive period (LRP) there were 40 people, and in the granulation and epithelization period (GEP) there were 15 people. There was a persistent increase in the concentration of the early, late reactive periods and the period of granulation and epithelization. In red blood cells, the content of intermediate intermediates of free radical lipid oxidation increases. The catalase activity in erythrocytes of victims with frostbite of the extremities decreases, but does not change in the blood serum. The dynamics of the serum in patients at different times of local cold injury in comparison with healthy people is not observed.

Sequence of increasing local cold injury

1951 ◽  
Author(s):  
Josef Pichotka ◽  
R. B. Lewis ◽  
Ella Freytag
Keyword(s):  
Cold Injury ◽  
Local Cold Injury

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

scholarly journals Chemical compositions, antimicrobial effects, and cytotoxicity of Asia minor wormwood (Artemisia splendens Willd.) growing in Iran

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Fariba Heshmati Afshar ◽  
Masumeh Zadehkamand ◽  
Zahra Rezaei ◽  
Abbas Delazar ◽  
Vahideh Tarhriz ◽  
...  
Keyword(s):  
Cell Line ◽  
Proliferative Activity ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Flavonoid Glycosides ◽  
Control Group ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Cytotoxicity Activity ◽  
Methanolic Extracts

Abstract Background Artemisia splendens from the Asteraceae family is a new source of biologically active compounds. The current study investigated to evaluate antimicrobial and cytotoxicity activity of methanolic extracts and their fractions obtained from aerial parts by agar disk diffusion and MTT methods, respectively. The active fractions were subjected to preparative HPLC for isolating the pure compounds, which were structurally elucidated, by 1H and 13C NMR. Results The results showed that the methanolic extract and its 60% SPE fraction have the anti-proliferative activity on A549 cell line in comparison with the control group. Meanwhile, the methanolic extract and its 40% SPE fraction can inhibit the growth of Gram-positive strains as anti-microbial activity. The 60% SPE fraction also illustrated anti-proliferative activity on the HT-29 cell line compared to the control group. Chromatographic separations via preparative HPLC yielded 5 flavonoids and three flavonoid glycosides. Conclusion Based on the results it can be concluded that A. splendens as a potential source of cytotoxic and antimicrobial compounds can be used in pharmaceutics.

A NEW APPROACH FOR INVESTIGATING HYPOTHALAMO-PITUITARY-GONADAL AND ADRENAL FEEDBACK CONTROL MECHANISMS: ACTIVE IMMUNIZATION WITH STEROIDS

1974 ◽  
Vol 76 (3) ◽  
pp. 556-569 ◽  
Author(s):  
E. Nieschlag ◽  
K. H. Usadel ◽  
H. K. Kley ◽  
U. Schwedes ◽  
K. Schöffling ◽  
...  
Keyword(s):  
Feedback Control ◽  
Leydig Cells ◽  
Positive Response ◽  
Active Immunization ◽  
Biologically Active ◽  
Control Group ◽  
Control Mechanisms ◽  
New Approach ◽  
Biological Neutralization ◽  
Group Serum

ABSTRACT A new method for the investigation of hypothalamo-pituitary-gonadal and adrenal feedback control mechanisms based on the biological neutralization of gonadal and adrenal steroids by active immunization is proposed. The regulatory influence of a given steroid in the feedback control is proved when reduction of the free, biologically active fraction of this steroid caused by antibody binding induces a positive response of the pituitary, thus effecting gonadal or adrenal hypertrophy and hyperfunction. The advantages and limitations of the new model are demonstrated by the effects of active immunization of rabbits with cortisol (F), aldosterone (Aldo), dehydroepiandrosterone (DHA), androstenedione (Δ4-A), testosterone (T), 5α-dihydrotestosterone (5α-DHT), 5β-DHT and oestradiol (E2). In the immunized animals and in a control group serum concentrations of total corticosteroids (TC), DHA, T, Δ4-A, E1, E2, LH and FSH, the percentage of binding of steroids in serum and the specificity of the antisera are determined. The testes are evaluated by histometry and the nuclear volume of the adrenocortical and Leydig cells is measured.

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