scholarly journals Stereological and Histopathological Effects of Trigonella foenum-graecum Seeds on Rabbits Ovary

2020 ◽  
Vol 44 ((E0)) ◽  
pp. 28-32
Author(s):  
Tamara N. Dawood
Keyword(s):  
The Other ◽  
Control Group ◽  
Female Gonad ◽  
Trigonella Foenum Graecum ◽  
Histological Sections ◽  
Vascular Congestion ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain ◽  
Positive Effect ◽  
Histopathological Effects

This study aimed to investigate the impacts of the Trigonella foenum-graecum (T. foenum-graecum) seeds on the female gonad. A total of twenty local rabbits were used in this study; were divided into four groups (5 each): first group (G1) was considered as the control group. The second group (G2), third group (G3) and fourth group (G4) were fed daily1.5%, 3%, and 4.5% of T. foenum-graecum seeds respectively for 60 days (twice daily). At the end of the experiment, the animals were euthanized by diethyl ether (C2H52O). Then the abdomen was incised, and the samples of ovaries were collected and fixed by 10% neutral buffered formalin. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with Hematoxylin and Eosin stain. The result showed that the numbers of primary and secondary follicles were significantly P< 0.05 decreased in G3and G4 compared with the control (G1) and G2. The numbers of Graafian follicles were significantly P<0.05 decreased G4 compared with other groups. The diameters of the primary, secondary, and Graafian follicles were significantly lower than the other groups. The thickness of the granulosa cell layer in G3and G4 were significantly lower than the other groups. The histological figures declared that the ovary of G2 was similar to that in G1. The histological sections of G3 and G4 were revealed marked cortical and medullary vascular congestion and focal hemorrhage; there were also marked follicular degeneration and cystic necrosis. The study concluded that the low concentration of T. foenum-graecum (fenugreek) seeds do not have any positive effect in terms of ovarian stimulation

scholarly journals The Repercussion of Ai Chi on Aged People’s Balance

2014 ◽  
Vol 4 (3) ◽  
pp. 247-256
Author(s):  
Pablo Javier Olabe Sánchez ◽  
Andrés Martínez-Almagro Andreo
Keyword(s):  
Fall Prevention ◽  
Controlled Trial ◽  
Intervention Group ◽  
The Other ◽  
Control Group ◽  
Aged People ◽  
Randomized Controlled ◽  
Risk Of Fall ◽  
And Control ◽  
Positive Effect

To practice strength and balance activities are shown as useful tools to fall prevention by diverse metanalysis. On the other side, aquatic environment offers interesting properties for a therapeutic reeducation, being the Ai Chi one of its applications. Aim: To evaluate the repercussion of an Ai Chi programme on aged people’s balance. Method: A randomized controlled trial was developed comparing two groups formed by aged people with risk of fall according to the Timed Up & Go test. Intervention Group (IG) followed twelve Ai Chi sessions combined with their thermal programme; and Control Group (CG) just followed the thermal programme. Both groups spent two weeks in the Balneario of Archena (Murcia, Spain). Results: Both groups improve their balance; however, only the IG reached the non-risk of fall from the Timed Up & Go test. Conclusions: The combination of Ai Chi sessions and a thermal programme had a positive effect on aged people’s balance, showing a therapeutic and clinical relevance.

The influence of low-frequency laser emission on phyto- and zooplankton productivity properties

2020 ◽  
Vol 12 (2) ◽  
pp. 196-201
Author(s):  
Lidiia Khuda ◽  
Larysa Cheban ◽  
Oleksii Khudyi
Keyword(s):  
Population Density ◽  
Catalase Activity ◽  
Laser Emission ◽  
Low Frequency ◽  
Positive Influence ◽  
The Other ◽  
Control Group ◽  
Microalgae Biomass ◽  
Frequency Laser ◽  
Positive Effect

We studied the possibility of using low frequency laser emission to improve production properties of feed hydrobionts, in particular microalgae Desmodesmus armatus and Daphnia magna, which are highly applied in aquaculture. It was shown that low-frequency laser emission with the wavelength 650 nm has a positive influence on production properties of both phyto- and zooplankton. In 24 hours the biomass of irradiated D. armatus was 25% larger than non-irradiated one. Meanwhile, application λ 420 and 530 nm did not show credible accumulation of microalgae biomass. If it comes to daphnia, positive dynamic in population density enlarging was observed only since 5-7th day of cultivation, depending on emission exposition. Differences in low-frequency laser emission with λ 420 and 530 efficacy on D. magna production properties were observed while using different exposition. Thus, maximal culture density was observed when λ 420 nm for 90 s and λ 650 nm for 60 s were applied. Longer irradiation with red laser did not have positive effect. It was established that in daphnia tissues, which were influenced by λ 420 nm laser emission, TBA-active products were accumulating more. Meanwhile, for daphnias, which were irradiated with red laser, TBA amount was not different from the control group one. Catalase activity increased while applying λ 650 nm laser emission. On the other hand, λ 420 nm did not cause credible increasing of catalase activity in comparison with control group. Taking received results about photobiological effects of low-frequency laser emission on D. armatus and D. magna into consideration, both biomasses enlargement during co-cultivation can be obtained if wavelength 650 nm is used.

Experimental Subarachnoid Hemorrhage in Rats 

1997 ◽  
Vol 87 (6) ◽  
pp. 1486-1493 ◽  
Author(s):  
Daniel J. Cole ◽  
Jeffrey C. Nary ◽  
Lowell W. Reynolds ◽  
Piyush M. Patel ◽  
John C. Drummond
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Subarachnoid Hemorrhage ◽  
Neuronal Death ◽  
Autologous Blood ◽  
The Other ◽  
Cerebral Hypoperfusion ◽  
Control Group ◽  
Experimental Subarachnoid Hemorrhage ◽  
Hematoxylin And Eosin

Background Hemodilution with diaspirin crosslinked hemoglobin (DCLHb) ameliorates occlusive cerebral ischemia. However, subarachnoid hemoglobin has been implicated as a cause of cerebral hypoperfusion. The effect of intravenous DCLHb on cerebral perfusion and neuronal death after experimental subarachnoid hemorrhage was evaluated. Methods Rats (n = 48) were anesthetized with isoflurane and subarachnoid hemorrhage was induced by injecting 0.3 ml of autologous blood into the cistema magna. Each animal received one of the following regimens: Control, no hematocrit manipulation; DCLHb, hematocrit concentration decreased to 30% with DCLHb; or Alb, hematocrit concentration decreased to 30% with human serum albumin. The experiments had two parts, A and B. In part A, after 20 min, cerebral blood flow (CBF) was assessed with 14C-iodoantipyrine autoradiography. In part B, after 96 h, in separate animals, the number of dead neurons was determined in predetermined coronal sections by hematoxylin and eosin staining. Results Cerebral blood flow was greater for the DCLHb group than for the control group; and CBF was greater for the Alb group than the other two groups (P &lt; 0.05). In one section, CBF was 45.5 +/- 10.9 ml x 100 g(-1) x min(-1) (mean +/- SD) for the control group, 95.3 +/- 16.6 ml x 100 g(-1) x min(-1) for the DCLHb group, and 138.1 +/- 18.7 ml x 100 g(-1) x min(-1) for the Alb group. The number of dead neurons was less in the Alb group (611 +/- 84) than in the control group (1,097 +/- 211), and was less in the DCLHb group (305 +/- 38) than in the other two groups (P &lt; 0.05). Conclusions These data support a hypothesis that hemodilution decreases hypoperfusion and neuronal death after subarachnoid hemorrhage. The data do not support the notion that intravascular molecular hemoglobin has an adverse effect on brain injury after subarachnoid hemorrhage.

scholarly journals The Value of Cytology Smears forAcanthamoebaKeratitis

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Sangita P. Patel ◽  
Jamie L. Schaefer ◽  
Ryan Jaber ◽  
Joyce Paterson ◽  
Weiguo Liu ◽  
...  
Keyword(s):  
The Other ◽  
Slit Lamp ◽  
Microscope Slide ◽  
Slit Lamp Examination ◽  
Difficult Diagnosis ◽  
Eye Pain ◽  
Peripheral Ring ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Purpose.Acanthamoebakeratitis remains a difficult diagnosis despite advances in genetic and imaging technologies. The purpose of this paper is to highlight the utility of cytology smears for diagnosis ofAcanthamoebakeratitis.Methods. This is a case study of the diagnostic course for a patient with suspectedAcanthamoebakeratitis.Results. A 40-year-old male with poor contact lens hygiene presented with severe left eye pain. Slit lamp examination showed two peripheral ring infiltrates without an epithelial defect. The epithelium over both infiltrates was removed with a Kimura spatula. Half of the sample was smeared on a dry microscope slide and the other half was submitted forAcanthamoebaculture and PCR. Both culture and PCR were negative forAcanthamoeba, but hematoxylin and eosin stain of the smear revealed double-walled cysts.Conclusion. H&E staining of corneal cytology specimens is an efficient and readily available test for diagnosis ofAcanthamoebakeratitis.

scholarly journals Research in the precaution of recombinant human erythropoietin to steroid-induced osteonecrosis of the rat femoral head

2017 ◽  
Vol 45 (4) ◽  
pp. 1324-1331 ◽  
Author(s):  
Lu-yong Jiang ◽  
Xiao Yu ◽  
Qing-jiang Pang
Keyword(s):  
Femoral Head ◽  
Recombinant Human Erythropoietin ◽  
Positive Control ◽  
The Other ◽  
Positive Control Group ◽  
Control Group ◽  
Human Erythropoietin ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Experimental Group

Objective To elucidate the effects of recombinant human erythropoietin (rHuEPO) on steroid-induced osteonecrosis of the femoral head in rats. Methods Twenty-four adult Wistar rats were randomly divided into three groups of eight rats each. The rats in the positive control group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks. The rats in the negative control group were injected with sodium chloride alone. The rats in the experimental group were injected with dexamethasone at 1 mg/kg twice a week for 5 weeks and rHuEPO (500 u/d/kg) daily for 5 weeks. The femoral head on one side was examined by hematoxylin and eosin staining, and that on the other side was examined by CD31 staining of the capillaries. Results Hematoxylin and eosin staining in the positive control group showed that the bony trabeculae had become obviously narrow and sparse with discontinuity of the integrity. The integrity of the trabeculae was better in the experimental group than positive control group. The CD31 expression was lower in the positive control group than in the other two groups. Conclusion rHuEPO can effectively prevent osteocyte apoptosis, delaying or decreasing osteonecrosis of the femoral head.

scholarly journals The effect of Ferbanat L nano-fertilizer on the growing of Petunia x grandiflora ’Musica Blue’

2014 ◽  
Vol 20 (3-4) ◽  
Author(s):  
Sz. Kisvarga ◽  
R. Kerezsi ◽  
I. Kohut ◽  
A. Tillyné Mándy
Keyword(s):  
Chlorophyll Content ◽  
Shoot Length ◽  
The Other ◽  
Culture Period ◽  
Control Group ◽  
Flower Buds ◽  
Treatment Groups ◽  
Remarkable Increase ◽  
Size Number ◽  
Positive Effect

During our experiment, the effect of Ferbanat L concentrations were examined by Petunia x grandiflora ‘Musica Blue’ production. The leaf and shoot length, number of flower buds, diameter of flowers, and the date of appearing the first buds were measured. The solution of Ferbanat L in 0.1 % concentration was the most effective on shoot length (21.7 cm). Remarkable increase was observed by the other treatment groups as well comparing to the control group (5.6 cm), the plants treated with solution of Ferbanat L 0.2% (16.5 cm) and 0.3% (14.4 cm) had significant effect as well. The nano-fertilizer had not positive effect on the other examined parameters as leaf and flower size, number of flower buds or chlorophyll content. The culture period shortened five days.

scholarly journals B6 Mouse Strain: The Best Fit for LPS-Induced Interstitial Cystitis Model

2021 ◽  
Vol 22 (21) ◽  
pp. 12053
Author(s):  
Ching-Hao Chen ◽  
Chun-Hou Liao ◽  
Kuo-Chiang Chen ◽  
Kuan-Lin Wang ◽  
Xiao-Wen Tseng ◽  
...  
Keyword(s):  
Interstitial Cystitis ◽  
Mouse Strain ◽  
Chronic Inflammatory Disease ◽  
Bladder Function ◽  
Mouse Strains ◽  
Control Group ◽  
Bladder Pain ◽  
Model Studies ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Stain

Interstitial cystitis (IC) is a chronic inflammatory disease characterized by bladder pain and increased urinary frequency. Although the C57BL/6J (B6) and FVB/NJ (FVB) mouse strains are commonly used as animal models for studies involving the urinary system, few reports have compared their lower urinary tract anatomy, despite the importance of such data. Our study aimed to characterize bladder function changes in FVB and B6 mouse strains with lipopolysaccharide (LPS)-induced IC, to understand mouse model-based bladder research. The bladder function parameters were measured by cystometrogram. Histological assay was examined by hematoxylin and eosin stain, Masson’s trichrome stain, and immunofluorescence staining. Results indicated that the two strains in the control group exhibited different bladder structures and functions, with significant anatomical differences, including a larger bladder size in the FVB than in the B6 strain. Furthermore, cystometry tests revealed differences in bladder function pressure. LPS-treated B6 mice presented significant changes in peak pressure, with decreased intercontraction intervals; these results were similar to symptoms of IC in humans. Each strain displayed distinct characteristics, emphasizing the care required in choosing the appropriate strain for bladder-model studies. The results suggested that the B6 mouse strain is more suitable for IC models.

scholarly journals Investigation of The Biochemical, and Histopathological Effects of Alpinia Officinarum Rhizome Extract Against Cisplatin-Induced Hepatotoxicity in Rats

2021 ◽  
Author(s):  
Atefeh Ashtari ◽  
Firoozeh Niazvand ◽  
Mahdi Azari ◽  
Narges Chamkouri
Keyword(s):  
Liver Damage ◽  
Biochemical Marker ◽  
The Other ◽  
Male Rats ◽  
Control Group ◽  
Marker Enzymes ◽  
Severe Liver ◽  
Alpinia Officinarum ◽  
Severe Liver Damage ◽  
Histopathological Effects

Abstract This study aimed to evaluate the effect of Alpinia officinarum rhizome extract (AORE) on cisplatin (CP)-induced hepatotoxicity in rats. In this regard, 44 male rats were divided into six groups including the control group, AORE control group, CP control group, and three groups of CP (7 mg/kg dose, on the tenth day) with AORE (at concentrations of 100, 200 and 400 mg/kg, daily for 14 days). After 14 days, the rats' livers were removed and their liver function was assessed using biochemical marker enzymes. Oxidative stress was assessed by evaluating malondialdehyde concentration and hepatic superoxide dismutase activity and a histopathological test was also conducted. According to the results, the group treated with CP had severe liver damage and the group treated with AORE had no harm compared with the control group. Among the groups receiving CP and AORE (at different concentrations), the group with 200 mg/kg AORE showed the lowest liver damage compared to the other two groups.

scholarly journals The Effect of Sea Lettuce (Ulva lactuca) Liquid Fertilizer and Zeolite Combinations on the Development of Cucumber (Cucumis sativus)

2019 ◽  
Vol 7 (7) ◽  
pp. 1021
Author(s):  
Mustafa Türkmen ◽  
Ayşe Su
Keyword(s):  
Cucumis Sativus ◽  
Organic Liquid ◽  
Germination Rate ◽  
Ulva Lactuca ◽  
The Other ◽  
Control Group ◽  
Liquid Fertilizer ◽  
Positive Effects ◽  
Fertilizer Material ◽  
Positive Effect

The combinations of organic liquid fertilizer material obtained from sea lettuce (Ulva lactuca) and different concentrations of zeolite were applied to the cucumber (Cucumis sativus) plant in six treatments with three replicates each. The combinations of the experimental groups were as follows; control group, G1 group 180 g Z (Zeolite), G2 group 90 g Z + 1140 ml Liquid Fertilizer (LF), G3 group 180 g Z + 620 ml LF, G4 group 270 g Z + 310 ml LF, G5 group 620 ml LF. Except the control group, all zeolites were applied with the first dose of liquid fertilizer, and other liquid fertilizer doses were given in three periods, after germination, flowering and yield. While the lowest germination rate was in control group, the highest rate was in group with 60 g zeolite. While the lowest seedling weight was observed in group with 90 g zeolite + 1140 ml liquid fertilizer, the highest rate was in group with 180 g zeolite + 620 ml liquid fertilizer. While the highest cucumber weight was observed in group with 270 g zeolite + 310 ml liquid fertilizer, the lowest was in group with 620 ml liquid fertilizer. Therefore, it may be said that zeolite has positive effect on germination. On the other hand, liquid fertilizer and zeolite combinations may have positive effects on the weight of the cucumber seedling and yield.

scholarly journals Environmentally persistant toxicant arsenic affects uterus grossly and histologically

2014 ◽  
Vol 11 (1) ◽  
pp. 61-68 ◽  
Author(s):  
MA Wares ◽  
MA Awal ◽  
SK Das ◽  
J Alam
Keyword(s):  
Female Genital Tract ◽  
Arsenic Exposure ◽  
Mature Female ◽  
Control Group ◽  
Veterinary Science ◽  
Uterine Gland ◽  
Hematoxylin And Eosin ◽  
Permanent Slide ◽  
Hematoxylin And Eosin Stain ◽  
Female Genital

The experiment was carried out to determine the gross and microscopic effects of arsenic on uterus of female Black Bengal Goats against control group in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202. A total of twelve mature female Black Bengal goats were studied, six from arsenic affected areas of Mymensingh district and theother six from Hill Tracts of Bangaldesh which are arsenic free. Gross parameters show slight variations in the morphology (color and shape) and biometry (size and weight) of uterus of arsenic affected Black Bengal goats, but this variation was statistically insignificant (p>0.05). For microscopic study, permanent slide was prepared by Mayer’s Hematoxylin and Eosin stain method. In microscopic level, there were significant variations in arsenic affected group of goats compared to the control group. Arsenic affected group of goats show thickening of uterine horn (p<0.01), uterine gland is lower in amount and diameter(p<0.01), cervical villi length was smaller (p<0.01), cervical wall thickness was higher (p<0.01). This work may be the tip of the iceberg representing the full extent of arsenic exposure to female genital tract. DOI: http://dx.doi.org/10.3329/bjvm.v11i1.13175 Bangl. J. Vet. Med. (2013). 11 (1): 61-68

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