scholarly journals A study of Serum Steroid Hormones Concentrations Of Pregnant Cows

2009 ◽  
Vol 33 (1) ◽  
pp. 180-182
Author(s):  
AL-Anbaky K. I. H.
Keyword(s):  
Blood Serum ◽  
Corpus Luteum ◽  
Steroid Hormones ◽  
Peripheral Blood ◽  
Hormone Level ◽  
Serum Concentrations ◽  
Jugular Veins ◽  
Serum Hormones ◽  
Blood Samples

The objective of present work is to estimated peripheral blood serum concentrations of pregnancyhormones, oestradiol , progesterone and testosterone , in cows . For this purpose 24 Frezain- Holsteincows at different stages of pregnancy the blood samples were taken from jugular veins. The serumwere separated and frozen at – 20 c until analysis. The serum hormones were measured by a specificELISA technique (ELISA Linear Multi Reader). The data were represented Mean + S.D. Progesteronewas high during pregnancy reaching a maximum of 91.94 + 26.09 ng/ml during last thirds (6-8months) of pregnancy , but was below 9.12 + 2.41 ng/ml for several months during the pregnancy.Oestradiol levels varied from 9.0+ 2.89 pg/ml in the first thirds of pregnancy to 282.6 + 48 .514 pg/mlduring the last month of gestation. While testosterone hormone level was low 0.32 + 0.12 ng /mlduring pregnancy. The result indicated that the major sources of hormones appeared to be the 0vary(corpus luteum ) and the uterus (placenta). The ovarian contribution was greater during the first – thirdsof pregnancy than later, whereas that made by the placenta was higher during the last thirds ofpregnancy

PROLACTIN IN MAINTENANCE OF THE CORPUS LUTEUM OF EARLY PSEUDOPREGNANCY IN THE GOLDEN HAMSTER

1981 ◽  
Vol 90 (2) ◽  
pp. 145-150 ◽  
Author(s):  
K. H. HARRIS ◽  
B. D. MURPHY
Keyword(s):  
Single Dose ◽  
Corpus Luteum ◽  
Golden Hamster ◽  
Peripheral Blood ◽  
Plasma Levels ◽  
Progesterone Level ◽  
Control Group ◽  
Blood Samples

The role of prolactin in the maintenance of the corpus luteum of pseudopregnancy was studied in the golden hamster. Nine groups of seven to fourteen animals each received 1 mg bromocriptine at 11.00 h on days 1, 2 or 3 of pseudopregnancy (three groups for each day). On each day of treatment with bromocriptine, one group of hamsters was injected with bovine prolactin 4 h before bromocriptine, and one group received prolactin 4 h before bromocriptine for three consecutive days following treatment with bromocriptine. One group received bromocriptine only. These nine groups were compared with a control group of animals given 0·85% saline instead of bromocriptine and prolactin. Peripheral blood samples were taken from all hamsters at 11.00 h on days 3, 4, 5 and 6 of pseudopregnancy and plasma levels of progesterone were determined by radioimmunoassay. Luteolysis, indicated by a decline in progesterone level by 24 or 48 h after treatment with bromocriptine, occurred in all hamsters given bromocriptine alone, whether it was administered on day 1, 2 or 3. Pretreatment with a single dose of prolactin did not mitigate the bromocriptine-induced fall in progesterone. In the majority of cases, pretreatment with prolactin plus daily doses of prolactin maintained the progesterone at levels not different from saline-treated hamsters. These data suggest that prolactin is a necessary luteotrophin during early pseudopregnancy without which luteolysis ensues.

Effects of an LH-releasing hormone antagonist on the secretion of LH, FSH, prolactin and ovarian steroids at different stages of the luteal phase in the stumptailed macaque (Macaca arctoides)

1986 ◽  
Vol 111 (1) ◽  
pp. 83-90 ◽  
Author(s):  
H. M. Fraser ◽  
M. Abbott ◽  
N. C. Laird ◽  
A. S. McNeilly ◽  
J. J. Nestor ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Luteal Phase ◽  
High Amplitude ◽  
High Serum ◽  
Serum Concentrations ◽  
Blood Samples ◽  
Luteal Function ◽  
Late Luteal Phase ◽  
Lhrh Antagonist ◽  
Lh Releasing Hormone

ABSTRACT The role of the pituitary gonadotrophins in controlling luteal function in the stumptailed macaque has been investigated by examining profiles of serum concentrations of LH, FSH, progesterone and oestradiol in daily blood samples from 13 monkeys during the menstrual cycle, and in blood samples taken at hourly intervals between 09.00 and 21.00 h on different days of the luteal phase in 13 cycles. The effects of acute withdrawal of gonadotrophins was investigated by administering a single injection of 300 μg LHRH antagonist/kg body weight at different stages of the luteal phase during 28 cycles. Although there were high basal values and marked fluctuations of bioactive LH during the first 4 days after the LH peak, progesterone profiles showed no corresponding short-term changes, there being a slow and steady rise in progesterone concentrations during the sampling periods. After day 5, basal LH secretion decreased, but high amplitude LH pulses were identified which were associated with episodes of progesterone secretion. Administration of the LHRH antagonist caused a suppression of bioactive LH and progesterone concentrations at all stages of the luteal phase, although some basal secretion of progesterone was maintained through the 24-h period of effective antagonist gonadotroph blockade. Luteal function recovered apparently normally in all monkeys treated in the early–mid-luteal phase. Serum concentrations of FSH and oestradiol fluctuated comparatively less during the 12-h sampling periods, and the antagonist had less suppressive effects on the concentrations of these hormones. The LHRH antagonist had no apparent effect on prolactin release. It appears that the corpus luteum is relatively unresponsive to the high serum LH concentrations during the early luteal phase, but that responsiveness increases as the corpus luteum develops. The corpus luteum is, however, susceptible to withdrawal of LH not only in the mid–late luteal phase when the relationship with LH is apparent, but also during the early luteal phase. J. Endocr. (1986) 111, 83–90

The concentration of progesterone, 20α-dihydroprogesterone, testosterone, oestrone and oestradiol-17β in serum, amniotic fluid and placental tissue of pregnant rabbits

1982 ◽  
Vol 99 (4) ◽  
pp. 605-611 ◽  
Author(s):  
I. F. Lau ◽  
S. K. Saksena ◽  
R. Salmonsen
Keyword(s):  
Amniotic Fluid ◽  
Blood Serum ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Placental Tissue ◽  
Maternal Tissue ◽  
Pregnant Rabbit ◽  
P Content ◽  
The Mean ◽  
Mean Concentration

Abstract. The concentrations of progesterone (Δ4P), 20α-dihydroprogesterone (20α-DHP), testosterone (T), oestrone (E1) and oestradiol-17β (E2β) in peripheral blood serum (PBS), amniotic fluid (AF) and placental tissue of rabbits during gestation were determined by radioimmunoassay. The placenta of the 10-day pregnant rabbit was fragile and composed mainly of maternal tissue. By the 12th day of pregnancy it was separable into maternal and foetal placentae. The mean concentration of Δ4P in PBS rose from 200 pg/ml (day 1 of pregnancy) to 17–21 ng/ml (days 10–15) and decreased gradually to 1 ng/ml a few hours before parturition. The 20α-DHP in PBS also showed an increase from 1.5 ng/ml (day 1) to 12 ng/ml (day 6) but fluctuated thereafter. The concentration of 20α-DHP in the PBS tended to be lower than that of Δ4P during pregnancy until the regression of the corpus luteum. An interesting observation was an increase of T on days 6–8 of pregnancy, the time when implantation occurs. The concentrations of E1 and E2β in PBS remained very low throughout pregnancy. Δ4P and 20α-DHP in AF ranged between 25 pg to 1 ng/ml and in no case during the course of pregnancy were the levels of T, E1 and E2β in AF higher than in PBS. Where the maternal placental Δ4P content remained between 1–2 ng/placenta, the foetal placenta Δ4P rose to a level of 15 ng/placenta by day 31 of pregnancy. A similar trend was recorded for 20α-DHP content. It is concluded that although a parallelism between PBS and myometrial steroid concentration was observed, no relationship could be drawn between the concentrations of steroid in PBS and those of the placental tissue and AF.

Pituitary Growth Hormone and the Question of Pancreatic Secretion of Glucagon

1956 ◽  
Vol 188 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Otakar V. Sirek ◽  
Anna Sirek ◽  
Charles H. Best
Keyword(s):  
Growth Hormone ◽  
Blood Sugar ◽  
Peripheral Blood ◽  
Blocking Agent ◽  
Diabetic Animal ◽  
Jugular Veins ◽  
Appreciable Change ◽  
Blood Samples ◽  
Transient Rise ◽  
Cast Doubt

Experiments are presented in which normal dogs were injected with a highly purified growth hormone preparation. One hour later blood samples were drawn simultaneously from the pancreatico-duodenal and jugular veins. The ‘central’ and ‘peripheral’ blood samples were injected into depancreatized dogs. While the injection of ‘peripheral’ blood produced no appreciable change in the blood sugar level of the diabetic recipient, the ‘central’ blood caused a definite but transient rise in blood sugar. The rise could be prevented by treating the recipient diabetic animal with the adrenergic blocking agent dihydroergotamine. It has been concluded that the hyperglycemic factor present in the blood of the pancreatico-duodenal vein after injection of growth hormone preparations is not identical with glucagon-Lilly, the effect of which is not influenced by dihydroergotamine. In two experiments in which completely depancreatized, instead of intact, dogs were used as donors the administration of growth hormone produced the characteristic hyperglycemic response when ‘central’ blood samples were injected into other depancreatized dogs. These findings cast doubt on the pancreatic origin of the hyperglycemic material and add interest to the search for its source and nature. Further experiments using depancreatized dogs are in progress.

scholarly journals Ultrasound Characteristics of the Cavitary Corpus Luteum after Oestrus Synchronization in Heifers in Relation to the Results of Embryo Transfer

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1706
Author(s):  
Bartłomiej Maria Jaśkowski ◽  
Hartwig Bostedt ◽  
Marek Gehrke ◽  
Jędrzej Maria Jaśkowski
Keyword(s):  
Blood Serum ◽  
Functional Status ◽  
Corpus Luteum ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Progesterone Level ◽  
Blood Samples ◽  
Tissue Area ◽  
Luteal Tissue ◽  
Ultrasound Assessment

The aim of the study was to conduct an ultrasound analysis of quantitative parameters of the corpus luteum (CL) in recipient heifers on days 6–8 after oestrus, and to compare reproduction potential of both types of CL in those females. Analyses were performed on 300 heifers, synchronized with two injections of cloprostenol. Clinical and ultrasound examinations of ovaries were performed and measurements of the CL were recorded. The blood samples were taken to determine progesterone level. Pregnancy examination was conducted after 6–8 weeks from the ET. Cavitary CL was found in 32.7% heifers In 48.0% of the cavitary CL, its luteal tissue area was reduced by 14.3% compared to the compact CL, while 16.3% of the CL had luteal tissue reduced by more than 33.8%. Progesterone level in blood serum was higher in heifers with the cavitary CL (p < 0.001). Pregnancy rate was higher for females with a cavitary CL (52%) than those with compact ones (33%, p < 0.05). The ultrasound assessment of luteal tissue should be included in the evaluation of the functional status of the CL in ET-recipient heifers. The cavitary CL presence may indicate a higher potential of the recipient in maintaining the pregnancy.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

scholarly journals Evaluation of T cell-mediated antitumor immune response in liver tumors - an approach using tumor and peripheral blood samples

HPB ◽  
2018 ◽  
Vol 20 ◽  
pp. S262
Author(s):  
R. Martins ◽  
C. Martín-Sierra ◽  
P. Laranjeira ◽  
A.M. Abrantes ◽  
J.G. Tralhão ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Peripheral Blood ◽  
Liver Tumors ◽  
Antitumor Immune Response ◽  
Blood Samples

scholarly journals Increased Density of Human Immunodeficiency Virus Type 1 Coreceptors CCR5 and CXCR4 on the Surfaces of CD4+ T Cells and Monocytes of Patients with Schistosoma mansoni Infection

2003 ◽  
Vol 71 (11) ◽  
pp. 6668-6671 ◽  
Author(s):  
W. Evan Secor ◽  
Amil Shah ◽  
Pauline M. N. Mwinzi ◽  
Bryson A. Ndenga ◽  
Caroline O. Watta ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Cell Surface ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Peripheral Blood ◽  
Chemokine Receptors ◽  
Blood Samples ◽  
Immunodeficiency Virus

ABSTRACT Distribution of chemokine receptors CCR5 and CXCR4, which are also coreceptors for human immunodeficiency virus type 1 invasion of cells, was measured on the surfaces of CD4+ T cells and monocytes in peripheral blood samples from a group of Kenyan car washers. Patients with active schistosomiasis displayed higher cell surface densities of these receptors than did cured schistosomiasis patients.

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