The concentration of progesterone, 20α-dihydroprogesterone, testosterone, oestrone and oestradiol-17β in serum, amniotic fluid and placental tissue of pregnant rabbits

1982 ◽  
Vol 99 (4) ◽  
pp. 605-611 ◽  
Author(s):  
I. F. Lau ◽  
S. K. Saksena ◽  
R. Salmonsen
Keyword(s):  
Amniotic Fluid ◽  
Blood Serum ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Placental Tissue ◽  
Maternal Tissue ◽  
Pregnant Rabbit ◽  
P Content ◽  
The Mean ◽  
Mean Concentration

Abstract. The concentrations of progesterone (Δ4P), 20α-dihydroprogesterone (20α-DHP), testosterone (T), oestrone (E1) and oestradiol-17β (E2β) in peripheral blood serum (PBS), amniotic fluid (AF) and placental tissue of rabbits during gestation were determined by radioimmunoassay. The placenta of the 10-day pregnant rabbit was fragile and composed mainly of maternal tissue. By the 12th day of pregnancy it was separable into maternal and foetal placentae. The mean concentration of Δ4P in PBS rose from 200 pg/ml (day 1 of pregnancy) to 17–21 ng/ml (days 10–15) and decreased gradually to 1 ng/ml a few hours before parturition. The 20α-DHP in PBS also showed an increase from 1.5 ng/ml (day 1) to 12 ng/ml (day 6) but fluctuated thereafter. The concentration of 20α-DHP in the PBS tended to be lower than that of Δ4P during pregnancy until the regression of the corpus luteum. An interesting observation was an increase of T on days 6–8 of pregnancy, the time when implantation occurs. The concentrations of E1 and E2β in PBS remained very low throughout pregnancy. Δ4P and 20α-DHP in AF ranged between 25 pg to 1 ng/ml and in no case during the course of pregnancy were the levels of T, E1 and E2β in AF higher than in PBS. Where the maternal placental Δ4P content remained between 1–2 ng/placenta, the foetal placenta Δ4P rose to a level of 15 ng/placenta by day 31 of pregnancy. A similar trend was recorded for 20α-DHP content. It is concluded that although a parallelism between PBS and myometrial steroid concentration was observed, no relationship could be drawn between the concentrations of steroid in PBS and those of the placental tissue and AF.

PROGESTERONE IN THE HUMAN PERIPHERAL BLOOD IN THE PREOVULATORY PERIOD OF THE MENSTRUAL CYCLE

1967 ◽  
Vol 55 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Benno Runnebaum ◽  
Josef Zander
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Progesterone Concentration ◽  
Plasma Progesterone ◽  
Progesterone Secretion ◽  
Graafian Follicle ◽  
The Mean ◽  
Mean Concentration

ABSTRACT Progesterone was determined and identified in human peripheral blood during the preovulatory period of the menstrual cycle, by combined isotope derivative and recrystallization analysis. The mean concentration of progesterone in 1.095 ml of plasma obtained 9 days before ovulation was 0.084 μg/100 ml. However, the mean concentration of progesterone in 1.122 ml of plasma obtained 4 days before ovulation was 0.279 μg/100 ml. These data demonstrate a source of progesterone secretion other than the corpus luteum. The higher plasma-progesterone concentration 4 days before ovulation may indicate progesterone secretion of the ripening Graafian follicle of the ovary.

scholarly journals A study of Serum Steroid Hormones Concentrations Of Pregnant Cows

2009 ◽  
Vol 33 (1) ◽  
pp. 180-182
Author(s):  
AL-Anbaky K. I. H.
Keyword(s):  
Blood Serum ◽  
Corpus Luteum ◽  
Steroid Hormones ◽  
Peripheral Blood ◽  
Hormone Level ◽  
Serum Concentrations ◽  
Jugular Veins ◽  
Serum Hormones ◽  
Blood Samples

The objective of present work is to estimated peripheral blood serum concentrations of pregnancyhormones, oestradiol , progesterone and testosterone , in cows . For this purpose 24 Frezain- Holsteincows at different stages of pregnancy the blood samples were taken from jugular veins. The serumwere separated and frozen at – 20 c until analysis. The serum hormones were measured by a specificELISA technique (ELISA Linear Multi Reader). The data were represented Mean + S.D. Progesteronewas high during pregnancy reaching a maximum of 91.94 + 26.09 ng/ml during last thirds (6-8months) of pregnancy , but was below 9.12 + 2.41 ng/ml for several months during the pregnancy.Oestradiol levels varied from 9.0+ 2.89 pg/ml in the first thirds of pregnancy to 282.6 + 48 .514 pg/mlduring the last month of gestation. While testosterone hormone level was low 0.32 + 0.12 ng /mlduring pregnancy. The result indicated that the major sources of hormones appeared to be the 0vary(corpus luteum ) and the uterus (placenta). The ovarian contribution was greater during the first – thirdsof pregnancy than later, whereas that made by the placenta was higher during the last thirds ofpregnancy

scholarly journals Plasma PGFM and progesterone concentrations, luteolysis moment and estrous cycle length in Nelore cows submitted to uterine biopsies

2015 ◽  
Vol 67 (5) ◽  
pp. 1210-1216
Author(s):  
I. Martin ◽  
C. M. B. Membrive ◽  
L. F. Vettorato ◽  
M. Binelli ◽  
E. Oba ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Estrous Cycle ◽  
Mean Value ◽  
Endometrial Biopsy ◽  
Control Group ◽  
Minimum Concentration ◽  
Before And After ◽  
The Mean ◽  
And Control ◽  
Mean Concentration

ABSTRACTIn this study, endometrial samples were collected in 14 Nelore cows on days zero (ovulation), five, nine, thirteen and nineteen of the estrous cycle (biopsy group), and in 15 females these collections weren't performed (control group). Biopsies were done on the uterine horn endometrium contralateral to the ovary with corpus luteum. Blood samples were taken at -24, -16, -8, 0 +8, +16 and +24 hours in relation to progesterone drop (<1ng/mL, zero moment) and evaluated for 13, 14-dihydro-15-keto prostaglandin F2-alpha (PGFM) by radioimmunoassay (RIA). Plasma progesterone concentration was determined by RIA every 24 hours. Data were analyzed by ANOVA using the PROC GLM and MIXED of the SAS. The mean value for PGFM during the entire period evaluated was greater in the biopsy group. The mean concentration of PGFM at moment zero was not different between the groups; the mean concentration of PGFM was higher in the biopsy group before and after the drop in progesterone level. The maximum mean concentration observed was not different between the groups; however, the mean minimum concentration was different with high values in the biopsy group. Although the PGFM concentrations were higher in the biopsy group, the biopsy and control groups had similar length of estrous cycle showing that repeated endometrial biopsy on the side contralateral to the ovary with corpus luteum does not affect luteolysis and the length of the estrous cycle.

Biochemical composition of amniotic fluid during different stages of gestation in Gaddi sheep

2018 ◽  
Author(s):  
Parul Shukla ◽  
Rajesh Rajput ◽  
R. Kumar ◽  
Madan Verma
Keyword(s):  
Alkaline Phosphatase ◽  
Amniotic Fluid ◽  
Stage Iv ◽  
Stage I ◽  
Stage Iii ◽  
Crown Rump Length ◽  
Healthy State ◽  
The Mean ◽  
Mean Concentration ◽  
I 31

The study was conducted on amniotic fluid collected from fifty gravid uteri of Gaddi sheep at different stages of gestation. The foetii were measured for their crown rump length and then divided into 4 stages i.e. stage I (31-60 days), stage II (61-90 days), stage III (91-120 days) and stage IV (121- till term). The mean concentration of glucose showed a declining trend from stage I to stage IV of gestation. The level of total protein remained consistent initially and decreased slightly in later stages of gestation. Creatinine and urea increased from stage I and II to stage III of gestation. Activity of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) was significantly higher in stage I of gestation. Alkaline Phosphatase showed highest activity in stage III of gestation. Changes in the concentration of glucose, urea, proteins or enzymes in foetal fluid reflect alterations in the functioning of liver during pregnancy and can be used as an important tool to assess the healthy state of pregnancy.

scholarly journals ASSESSMENT OF MINERAL STATUS OF TWICE-YEARLY LAMBING YANKASA EWES 1. APRAISAL OF MINERAL STATUS IN THE DIET AND BLOOD SERUM

2021 ◽  
Vol 16 ◽  
pp. 20-27
Author(s):  
B. A. Fajemisin
Keyword(s):  
Blood Serum ◽  
Dry Matter ◽  
Mineral Deficiency ◽  
Mineral Status ◽  
Mineral Profile ◽  
Baseline Information ◽  
The Mean ◽  
Calcium And Phosphorus ◽  
Iron Manganese ◽  
Mean Concentration

An appraisal of mineral status of twice-yearly lambing Yankasa ewes was carried out in order to trace any evidence of mineral deficiency in the diet and to obtain baseline information of clinical, physiological and nutritional interest. A total of thirty pregnant and lactation ewes, weighing between 27 - 37kg was sampled for mineral profile in blood serum. The basal and supplemental diets were also analysed for chemical and mineral composition. Average daily dry matter intakes ranged from 0.92 to 1.5kg per head per day. Higher concentration of magnesium, iron, manganese and zinc were obtained in the serum of both pregnant and lactating ewes than values obtained for temperate breeds. Average calcium concentrations (mg/100ml) in the blood serum during gestation and lactation were 10.0 ± 1.7 and 6.1 æ 1.3respectively. The values were higher during gestation than lactation. The differences were significant (P 0.05). On the other hand, the mean concentration (mg/100ml) was 12.6 æ 2.2 during gestation and increased to 17.4 æ 3.2 during lactation. The differences were also significant. (P < 0.01). The concentrations of calcium and phosphorus (mg/100ml) were observed to be influenced by parturition.

scholarly journals The concentrations of selected blood serum proteins in calves during the first three months of life

2016 ◽  
Vol 85 (1) ◽  
pp. 33-40 ◽  
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Veronika Nagyová ◽  
Gabriel Kováč
Keyword(s):  
Blood Serum ◽  
Serum Proteins ◽  
Fold Increase ◽  
Postnatal Period ◽  
Gradual Decrease ◽  
Serum Concentrations ◽  
Blood Serum Proteins ◽  
The Mean ◽  
Mean Concentration ◽  
Precise Interpretation

This study was aimed at the evaluation of changes in the concentrations of selected blood serum proteins in calves during the first three months of life, including precolostral, colostral, milk, transitional and solid feeding period. Nine clinically healthy calves were used in this study. The first blood sampling was performed before the colostrum intake (day 0) and then at 1, 2, 7, 14, 30, 60, and 90 days of age. Blood serum was analyzed for the concentrations of α1-fetoprotein, prealbumin, transferrin, and lactoferrin. The results showed significant changes in the serum concentrations of all the evaluated proteins in calves during the first three months of life (P< 0.01 andP< 0.001). At birth, the mean concentration of α1-fetoprotein was low and increased more than × 8 at one day after colostrum intake, then a gradual decrease was found up to day 30 of life. Similar tendency was observed in the concentrations of prealbumin and lactoferrin. While the concentrations of prealbumin increased approximately × 3.5 at one day after colostrum intake, the serum lactoferrin values showed approximately 1.5-fold increase. A subsequent gradual decrease from the 2ndday of life was found for both prealbumin and lactoferrin. In the concentrations of transferrin, a significant increase was observed on day 7 (P< 0.05). The highest values were found at the age of 14 and 30 days. These results suggest that concentrations of these proteins in calves are markedly altered during the postnatal period, which should be taken into consideration for their precise interpretation in young animals.

Instant 99mTc-Labelled Glucoheptonate Kit for Kidney Imaging

1983 ◽  
Vol 22 (05) ◽  
pp. 246-250 ◽  
Author(s):  
M. Al-Hilli ◽  
H. M. A. Karim ◽  
M. H. S. Al-Hissoni ◽  
M. N. Jassim ◽  
N. H. Agha
Keyword(s):  
Sodium Hydroxide Solution ◽  
Normal Subjects ◽  
Organ Distribution ◽  
Stable Complex ◽  
Scintillation Camera ◽  
Ph Adjustment ◽  
The Mean ◽  
The Stability ◽  
Kidney Imaging ◽  
Mean Concentration

Gelchromatography column scanning has been used to study the fractions of reduced hydrolyzed 99mTc, 99mTc-pertechnetate and 99mTc-chelate in a 99mTc-glucoheptonate (GH) preparation. A stable high labelling yield of 99mTc-GH complex in the radiopharmaceutical has been obtained with a concentration of 40-50 mg of glucoheptonic acid-calcium salt and not less than 0.45 mg of SnCl2 2 H2O at an optimal pH between 6.5 and 7.0. The stability of the complex has been found significantly affected when sodium hydroxide solution was used for the pH adjustment. However, an alternative procedure for final pH adjustment of the preparation has been investigated providing a stable complex for the usual period of time prior to the injection. The organ distribution and the blood clearance data of 99mTc-GH in rabbits were relatively similar to those reported earlier. The mean concentration of the radiopharmaceutical in both kidneys has been studied in normal subjects for one hour with a scintillation camera and the results were satisfactory.

Sonographic Assessment of Amniotic Fluid Volume using Single Deepest Pocket (SDP) among Igbo Women with Uncomplicated Singleton Pregnancies.

2019 ◽  
Vol 2 (1) ◽  
pp. 1-18
Author(s):  
Chijioke Okeudo ◽  
B.U. Ezem
Keyword(s):  
Amniotic Fluid ◽  
Gestational Age ◽  
Cross Sectional Study ◽  
Fluid Volume ◽  
Singleton Pregnancy ◽  
Cross Sectional ◽  
South Eastern ◽  
Amniotic Fluid Volume ◽  
The Mean ◽  
Singleton Pregnancies

Background: The amniotic fluid is fundamental for proper fetal development and growth. Ultrasound visualization of the amniotic fluid permits both subjective and objective estimates of the amniotic fluid. Objective: The objective of this study was to determine the reference values of normal single deepest pocket (SDP) – upper and lower limits, mean SDP and variation of the SDP with gestational age among Igbo women of South-Eastern Nigeria extraction carrying uncomplicated singleton pregnancy. Methodology: This was a prospective cross sectional study involving 400 women carrying uncomplicated singleton pregnancies and who were sure of the date of the first day of their last menstrual period. The single deepest pocket / maximum vertical pool were determined once at presentation at the hospital.. The study was conducted from January 1st to December 31st 2015. The second author carried out all the scanning. The SDP was obtained. Results: The womens’ mean and median ages were the same at 28 years. The gestational age range of the pregnancies was 14-41 weeks. The mean SDP was 5.8cm, while the 5th and 95th percentiles were 3.3cm and 8.5cm respectively. There was no difference in the mean SDP in both term and preterm. There was irregular but continuous rise of mean SDP to a peak of 6.8cm at gestational age of 39 weeks. In conclusion, the participants had a mean SDP of 5.8cm. There was also a positive correlation between SDP and Gestational age. We therefore recommend a longitudinal study to assess perinatal outcome and abnormal amniotic fluid volume among Igbo women of South-Eastern Nigeria. Key words: Single Deepest Pocket, Uncomplicated Singleton Pregnancy, Igbo Women.

PS-095a The Mean Platelet Volume In Infants With Meconium Stained Amniotic Fluid

2014 ◽  
Vol 99 (Suppl 2) ◽  
pp. A144.4-A145
Author(s):  
M Tekin ◽  
C Konca ◽  
Z Kahramaner ◽  
A Erdemir ◽  
A Gulyuz ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Mean Platelet Volume ◽  
Platelet Volume ◽  
The Mean ◽  
Meconium Stained Amniotic Fluid

scholarly journals The Relationship between Inflammation Markers (CRP, IL-6, sCD40L) and Colorectal Cancer Stage, Grade, Size and Location

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1382
Author(s):  
Olga Martyna Koper-Lenkiewicz ◽  
Violetta Dymicka-Piekarska ◽  
Anna Justyna Milewska ◽  
Justyna Zińczuk ◽  
Joanna Kamińska
Keyword(s):  
Colorectal Cancer ◽  
Linear Regression ◽  
Tumor Size ◽  
Distal Colon ◽  
Proximal Colon ◽  
Model Parameters ◽  
Grade Group ◽  
Who Grade ◽  
The Mean ◽  
Mean Concentration

The aim of the study was the evaluation whether in primary colorectal cancer (CRC) patients (n = 55): age, sex, TNM classification results, WHO grade, tumor location (proximal colon, distal colon, rectum), tumor size, platelet count (PLT), mean platelet volume (MPV), mean platelet component (MCP), levels of carcinoembryonic antigen (CEA), cancer antigen (CA 19-9), as well as soluble lectin adhesion molecules (L-, E-, and P-selectins) may influence circulating inflammatory biomarkers: IL-6, CRP, and sCD40L. We found that CRP concentration evaluation in routine clinical practice may have an advantage as a prognostic biomarker in CRC patients, as this protein the most comprehensively reflects clinicopathological features of the tumor. Univariate linear regression analysis revealed that in CRC patients: (1) with an increase in PLT by 10 × 103/μL, the mean concentration of CRP increases by 3.4%; (2) with an increase in CA 19-9 of 1 U/mL, the mean concentration of CRP increases by 0.7%; (3) with the WHO 2 grade, the mean CRP concentration increases 3.631 times relative to the WHO 1 grade group; (4) with the WHO 3 grade, the mean CRP concentration increases by 4.916 times relative to the WHO 1 grade group; (5) with metastases (T1-4N+M+) the mean CRP concentration increases 4.183 times compared to non-metastatic patients (T1-4N0M0); (6) with a tumor located in the proximal colon, the mean concentration of CRP increases 2.175 times compared to a tumor located in the distal colon; (7) in patients with tumor size > 3 cm, the CRP concentration is about 2 times higher than in patients with tumor size ≤ 3 cm. In the multivariate linear regression model, the variables that influence the mean CRP value in CRC patients included: WHO grade and tumor localization. R2 for the created model equals 0.50, which indicates that this model explains 50% of the variance in the dependent variable. In CRC subjects: (1) with the WHO 2 grade, the mean CRP concentration rises 3.924 times relative to the WHO 1 grade; (2) with the WHO 3 grade, the mean CRP concentration increases 4.721 times in relation to the WHO 1 grade; (3) with a tumor located in the rectum, the mean CRP concentration rises 2.139 times compared to a tumor located in the distal colon; (4) with a tumor located in the proximal colon, the mean concentration of CRP increases 1.998 times compared to the tumor located in the distal colon; if other model parameters are fixed.

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