scholarly journals The protective role of Pomegranate seed oil (Pometone) on serum protein in sodium fluoride treated female rats

2016 ◽  
Vol 40 (1) ◽  
pp. 61-68
Author(s):  
Baraa Najim Al-Okaily
Keyword(s):  
Drinking Water ◽  
Sodium Fluoride ◽  
Total Protein ◽  
Seed Oil ◽  
Protective Role ◽  
Female Rats ◽  
Serum Total Protein ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

This study is designed to determin the protective role of pomegranate seed oil against deleterious effect induced by sodium fluoride on serum proteins in rats. Forty (40) adult female rats were randomly divided into four equal group (10/group) and treated daily for 40 days as following: Group C administered distilled water (control), group T1 received sodium fluoride 120 ppm/liter in drinking water, group T2 received both sodium fluoride 120 ppm/liter in drinking water and Pometone 30 mg/kg B.w. orally and group T3 administered Pometone 30 mg/kg B.w. orally. Fasting blood samples were collected at 0, 20 and 40 days of the experimental periods and serum samples were aliquoted for estimation serum total protein, albumin and globulin concentrations. Also, protein electrophoresis was measured. The results revealed that sodium fluoride caused significant reduction in serum total protein, albumin and globulin concentrations, in addition to changes in the patterns of serum protein fractions % (albumin, α1-globulin, α2-globulin, β-globulin and γ-globulin) as compared with control rats. In conclusion Pomegranate seed oil caused elevation of proteins concentration as compared to group T1 and could potentially be beneficial in preventing the hepatic damage caused by sodium fluoride.  

scholarly journals Effect of Pomegranate Seed Oil against Hepatotoxicity- Induced by Sodium Fluoride in Adult Female Rats (Part II).

2019 ◽  
Vol 43 (1) ◽  
pp. 102-112
Author(s):  
Baraa Najim Al-Okaily
Keyword(s):  
Total Cholesterol ◽  
Adult Female ◽  
Sodium Fluoride ◽  
Seed Oil ◽  
Total Bilirubin ◽  
Tap Water ◽  
Female Rats ◽  
Control Group ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

This experiment was designed to evaluate the hepatoprotective effects of pomegranate seed oil against toxicity- induced by sodium fluoride and in normal rats. Twenty  adult female Wistar rats were divided into four equal group and treated daily for 40 days as follows: Group C administered  tap water and served as control , group T1 : received sodium fluoride 120ppm in drinking tap water, group T2: received both sodium fluoride 120ppm in drinking water and  administered orally pometone (pomegranate seed oil) 30mg/kg B.W. and group T3 : administered pomegranate seed oil as in group T2 orally. Fasting blood samples were collected at 0, 20 and 40 days to estimation of some biochemical parameters and oxidative stress biomarkers . In addition, sections from liver were taken at the end of the experiment for histopathological study. The results revealed that SF (group T1) caused a significant increase in serum aminotransferases (serum alanine aminotransferase and aspartate aminotransferase) activities, total cholesterol ,total bilirubin and peroxynitrite radical concentrations, while GSH concentration was a significantly decrease.  PSO caused an alleviation to the hepatic dysfunction induced by sodium fluoride (group T2) manifested through significant elevation of GSH concentration, in addition, a significant reduction in serum transaminases activity, total cholesterol, peroxynitrite radical and total bilirubin concentrations. In contrast, administration of pomegranate seed oil (group T3) showed no alterations in most of these parameters. Furthermore  histopathological examination of liver tissues of  group T1 manifested aggregation of mononucleated cells, proliferation of hepatocyte, cytoplasmic fat droplet and granulomatous lesion consists of aggregation of macrophage and lymphocyte. All these alteration in liver histology were modified by treatment of rats with pomegranate seed oil (group T2) and no pathological lesion was reported in group T3. On conclusion, this study documented the beneficial effect of pomegranate seed oil against the deleterious effects of SF on liver functions of adult female rats.

scholarly journals The Role of Pometone (Pomegranate seed oil) in Ameliorating the Deleterious Effect of Methionine Overload on Some Histological Aspects of heart and aorta in Female Rabbits (Part-II)

2014 ◽  
Vol 38 (1) ◽  
pp. 62-70
Author(s):  
Baraa Najim Al-Okaily
Keyword(s):  
Cardiac Muscle ◽  
Muscle Fiber ◽  
Seed Oil ◽  
Mononuclear Cells ◽  
Corn Oil ◽  
Control Group ◽  
Aortic Tissue ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

This experiment was aimed to investigate the role of pomegranate seed oil (PSO) in ameliorating the deleterious effects of methionine overload on some histopathological structure of heart and aorta in adult female rabbits. Thirty-Two female rabbits divided into four groups eight animals each, and treated for 42 days daily as follows: the first groups were drenched drinking corn oil, serving as control (group C), second group (group T1) were intubated orally with methionine 100mg/kg. B.W, while the third group (groupT2) were intubated orally with methionine 100mg/kg. B.W and pomegranate seed oil (PSO) 30 mg /Kg. B.W, and the animals in group T3 were intubated orally with pomegranate seed oil 30 mg /Kg. B.W. At the end of the experiment rabbits were sacrificed. Serial sections from the heart and aorta were prepared and examined microscopically. Histological examination of heart and aorta of methionine overload treated group (T1) showed edema ,RBCs and few neutrophils infiltration ,with vacuolar degeneration of cardiac muscle cells , fragment of muscle fiber, congested blood vessels between muscle fibers. An increase in thickness of intima, erosion and mononuclear cells infiltration in sub intima of aorta were also observed. Histological sections of heart and aorta in T2 and T3 groups showed the absence of histopathological lesions in aortic tissue with moderate edema between muscle fiber of T2 group as comparing to group T1. In conclusion, the results confirm the cardioprotective role of pomegranate seed oil by ameliorating the effect of methionine overload on cardiac muscle and aorta.

scholarly journals Pomegranate Seed Oil and Bitter Melon Extract Affect Fatty Acids Composition and Metabolism in Hepatic Tissue in Rats

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5232
Author(s):  
Agnieszka Stawarska ◽  
Tomasz Lepionka ◽  
Agnieszka Białek ◽  
Martyna Gawryjołek ◽  
Barbara Bobrowska-Korczak
Keyword(s):  
Fatty Acids ◽  
Dietary Supplements ◽  
Seed Oil ◽  
Dietary Factors ◽  
Female Rats ◽  
Hepatic Tissue ◽  
Bitter Melon ◽  
Fatty Acids Composition ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Pomegranate seed oil (PSO) and bitter melon dried fruits (BME) are used as natural remedies in folk medicine and as dietary supplements. However, the exact mechanism of their beneficial action is not known. The aim of study was to assess how the diet supplementation with PSO and/or with an aqueous solution of Momordica charantia affects the metabolism of fatty acids, fatty acids composition and the level of prostaglandin E2 (PGE2) in rat liver. Animals (Sprague-Dawley female rats, n = 48) were divide into four equinumerous groups and fed as a control diet or experimental diets supplemented with PSO, BME or both PSO and BME for 21 weeks. Fatty acids were determined using gas chromatography with flame ionization detection. PSO added to the diet increased the rumenic acid content (p < 0.0001) and increased accumulation of n-6 fatty acids (p = 0.0001) in hepatic tissue. Enrichment of the diet either with PSO or with BME reduced the activity of Δ6-desaturase (D6D) (p = 0.0019), whereas the combination of those dietary factors only slightly increased the effect. Applied dietary supplements significantly reduced the PGE2 level (p = 0.0021). No significant intensification of the influence on the investigated parameters resulted from combined application of PSO and BME. PSO and BME have potential health-promoting properties because they influence fatty acids composition and exhibit an inhibiting effect on the activity of desaturases and thus they contribute to the reduction in the metabolites of arachidonic acid (especially PGE2).

scholarly journals The Protective role of Pomegranate seed oil (Pometon) on kidney (functional and structural) damage induced by Methionine overload in adult female Rabbits

2014 ◽  
Vol 38 (2) ◽  
pp. 66-73
Author(s):  
Ahmed Dawood Salman
Keyword(s):  
Adult Female ◽  
Structural Damage ◽  
Seed Oil ◽  
Mononuclear Cells ◽  
Corn Oil ◽  
Protective Role ◽  
Structural Effect ◽  
Renal Tubules ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

     This study  was designed to investigate the protective effect of Pomegranate seed oil (PSO) on kidney damage induced by methionine overload in adult female rabbits. Twenty four  adult female local rabbits were randomly divided into four equal groups, and they were treated daily for 42 days,as fallow: the first group (C): rabbits  were received ordinary corn oil orally, serving as control, while aniamls in the second  group T1 were intubated with methionine (100 mg/kg BW) orally and animals in third group (T2)  were intubated with methionine (100mg/kg BW) plus pometon (30mg/kg BW) diluted in corn oil wihle, rabbits in fourth group (T3) were intubated with pometone (30 mg/kg BW) diluted in corn oil.Fasting blood samples were collected from all experimental groups at 0, 21, and 42 days of experiment to study the parameters: Serum creatinine (SC) concentration and Blood urea nitrogen (BUN) concentration. Sections of kidney were assessed for histopathological studies. The result revealed that exposure of animals to methionine in group (T1) for 42 days caused a significant increase (P<0.05) in SC, BUN, concentrations as comparing to pomegranate  treated groups and control. While oral intubation of PSO caused significant decrease (P<0.05) in the previous and correct the values near that of the control. Exposure to methionine overload showed severe acute cellular degeneration with   mononuclear cells infiltration between renal tubules while  there was no clear pathlogical lesions in kidney section after PSO intubation. In conclusion, it seems that  Pomegranate seed oil intubation possessed renoprotactive (functional and structural) effect against deleterious damage induced by Methionine overload  in rabbits.

In Vitro Estimation of Photo-Protective Potential of Pomegranate Seed Oil and Development of a Nanoformulation

2019 ◽  
Vol 15 (1) ◽  
pp. 87-102 ◽  
Author(s):  
Surbhi Dhawan ◽  
Sanju Nanda
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Seed Oil ◽  
Topical Delivery ◽  
Inflammatory Activity ◽  
Pomegranate Seed Oil ◽  
Protective Potential ◽  
Release Studies ◽  
Pomegranate Seed

Background: Since ancient times, people have been using natural resources for photoprotection purposes. One such highly recognised natural agent is pomegranate seed oil, considered as wonder oil owing to the presence of several beneficial phytoconstituents. </P><P> Objective: The study aimed to establish the photoprotective potential of pomegranate seed oil through various in vitro and biochemical studies along with the formation of nanoemulsion, an efficient topical delivery system for the oil. </P><P> Method: Photo-protective potential of the oil was estimated by determining in vitro antioxidant and anti-inflammatory activity, total phenolic content, anti elastase, antihyaluronidase and anticollagenase activities of the oil. Ultrasonication method was used to formulate nanoemulsions. The optimisation was done following the central composite design. The characterisation was done by particle size analysis, zeta potential, polydispersity index, pH, viscosity, stability testing and transmission electron microscopy. The optimised nanoemulsion was loaded into a gel base for topical application and further release studies were carried out. </P><P> Results: The IC50 values of anti-elastase, anti-collagenase and anti-hyaluronidase were found to be 309 mg/ml, 4 mg/ml and 95 mg/ml respectively. The results of anti-oxidant and anti-inflammatory activity were also significant, which thereby established the photo-protective potential of the oil. The optimum batch 2 had particle size 83.90 nm, 0.237 PDI and -5.37 mV zeta potential. The morphology was confirmed by TEM. Batch 2 was incorporated into a gel base and release studies showed 74.12 % release within 7 hours. </P><P> Conclusion: Pomegranate seed oil possesses a potential photo-protective ability. Nanoemulsions proved to be a promising carrier for the topical delivery of the oil.

scholarly journals Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Raghavendra Mitta ◽  
Sushmitha Duddu ◽  
Raghuveer Yadav Pulala ◽  
Pradeepkumar Bhupalam ◽  
Venkatakirankumar Mandlem ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Sodium Fluoride ◽  
Total Protein ◽  
Reduced Glutathione ◽  
Serum Biomarkers ◽  
Albino Rats ◽  
Serum Total Protein ◽  
Treatment Groups ◽  
Group I

AbstractObjectivesThe main objective of the present study is to evaluate the mitigative effect of hydroalcoholic extract of Momordica cymbalaria fruits against sodium fluoride (NaF) induced hepatotoxicity.MethodsIn this study, Wistar male albino rats were randomly divided into five groups of six rats each. Group I and II served as normal and toxic controls. Group III as plant control received extract at a dose of 400 mg/kg b. wt, p.o and Groups IV and V as treatment groups received extract at a dose 200 and 400 mg/kg b. wt, p.o for 30 days. All groups except Groups I and III received 100 ppm of NaF through drinking water. After completion of the study, blood collected for the estimation of liver blood serum biomarkers such as aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline Phosphatase (ALP), direct and total bilirubin, total protein and albumin. The liver tissue homogenate was for estimation of lipid peroxidation, catalase, and reduced glutathione levels.ResultsThe results showed that NaF intoxication caused elevation of liver blood serum levels and lipid peroxidation; decreased levels of serum total protein, albumin and liver reduced glutathione, and catalase observed. The treatment groups showed decreased elevated serum biomarkers (ALT, AST, and ALP), liver lipid peroxidation and increased serum total protein and albumin, liver reduced glutathione and catalase levels in a dose-dependent manner. Histopathological studies also further strongly supported for mitigative effects of the plant.ConclusionsIn conclusion, our findings of the study indicated that M. cymbalaria fruits were a potential drug candidate in the treatment of NaF induced hepatotoxicity.

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