scholarly journals Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Raghavendra Mitta ◽  
Sushmitha Duddu ◽  
Raghuveer Yadav Pulala ◽  
Pradeepkumar Bhupalam ◽  
Venkatakirankumar Mandlem ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Sodium Fluoride ◽  
Total Protein ◽  
Reduced Glutathione ◽  
Serum Biomarkers ◽  
Albino Rats ◽  
Serum Total Protein ◽  
Treatment Groups ◽  
Group I

AbstractObjectivesThe main objective of the present study is to evaluate the mitigative effect of hydroalcoholic extract of Momordica cymbalaria fruits against sodium fluoride (NaF) induced hepatotoxicity.MethodsIn this study, Wistar male albino rats were randomly divided into five groups of six rats each. Group I and II served as normal and toxic controls. Group III as plant control received extract at a dose of 400 mg/kg b. wt, p.o and Groups IV and V as treatment groups received extract at a dose 200 and 400 mg/kg b. wt, p.o for 30 days. All groups except Groups I and III received 100 ppm of NaF through drinking water. After completion of the study, blood collected for the estimation of liver blood serum biomarkers such as aspartate aminotransferases (AST), alanine aminotransferases (ALT), alkaline Phosphatase (ALP), direct and total bilirubin, total protein and albumin. The liver tissue homogenate was for estimation of lipid peroxidation, catalase, and reduced glutathione levels.ResultsThe results showed that NaF intoxication caused elevation of liver blood serum levels and lipid peroxidation; decreased levels of serum total protein, albumin and liver reduced glutathione, and catalase observed. The treatment groups showed decreased elevated serum biomarkers (ALT, AST, and ALP), liver lipid peroxidation and increased serum total protein and albumin, liver reduced glutathione and catalase levels in a dose-dependent manner. Histopathological studies also further strongly supported for mitigative effects of the plant.ConclusionsIn conclusion, our findings of the study indicated that M. cymbalaria fruits were a potential drug candidate in the treatment of NaF induced hepatotoxicity.

Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats

2017 ◽  
Vol 42 (4) ◽  
Author(s):  
Mahgoub Mohamed Ahmed
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Rat Liver ◽  
Adult Male ◽  
Total Protein ◽  
Albino Rats ◽  
Serum Total Protein

AbstractObjective:The objective of the current study is to investigate the protective effect ofMethods:Forty adult male albino rats were divided into four groups as control,Results:The results showed that, administration of EtOH caused a significant decrease (p<0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.Conclusion:had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver.

Bitter Melon Protects Against Lipid Peroxidation Caused by Immobilization Stress in Albino Rats

2009 ◽  
Vol 79 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Chaturvedi
Keyword(s):  
Lipid Peroxidation ◽  
Immobilization Stress ◽  
Reduced Glutathione ◽  
Cumene Hydroperoxide ◽  
Liver Homogenate ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Bitter Melon ◽  
Protective Effects

In the present study, protective effects of bitter melon (Momordica charantia) extract on lipid peroxidation induced by immobilization stress in rats have been assessed. Graded doses of extract (50, 100, and 150 mg/kg body weight) were administered orally to rats subjected to immobilization stress for two hours for seven consecutive days. Stress was applied by keeping the rats in a cage where no movement was possible. After seven days, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substances, reduced glutathione, and catalase. In vitro effects of M. charantia extract on lipid peroxidation in liver homogenate of normal, control, and rats pretreated with extract were carried out against cumene hydroperoxide-induced lipid peroxidation. Results reveal that in vivo M. charantia inhibited stress-induced lipid peroxidation by increasing the levels of reduced glutathione and activities of catalase. These results were further supported by in vitro results. In vitro inhibition of lipid peroxidation was indicated by low levels of thiobarbituric acid in the liver homogenate from pretreated rats and normal rats when incubated with both cumene hydroperoxide and extract. Inhibition was also noted in the homogenate where the rats were pretreated but the mixture contained no extract. Thus this plant provides protection by strengthening the antioxidants like reduced glutathione and catalase. Inclusion of this plant in the daily diet would be beneficial.

scholarly journals EFFICACY OF TRIDHAM AND 1,2,3,4,6-PENTA-O-GALLOYL-β-D-GLUCOSE IN REVERSING LIPID PEROXIDATION LEVELS AND MITOCHONDRIAL ANTIOXIDANT STATUS IN 7,12-DIMETHYLBENZENEANTHRACENE (DMBA) INDUCED BREAST CANCER IN SPRAGUE-DAWLEY RATS

2016 ◽  
Vol 8 (9) ◽  
pp. 288 ◽  
Author(s):  
Stalin Ramakrishnan ◽  
Karthick Dharmalingam ◽  
Sachidanandham T Panchanatham ◽  
Shanthi Palanivelu
Keyword(s):  
Lipid Peroxidation ◽  
Therapeutic Effect ◽  
Mammary Carcinoma ◽  
Antioxidant Status ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Sprague Dawley ◽  
Standard Drug ◽  
Gastric Intubation ◽  
Group I

<p><strong>Objective: </strong>To determine the effect of <em>Tridham</em> (TD) and 1,2,3,4,6-penta-O-galloyl-β-d-glucose(PGG) on lipid peroxidation levels and mitochondrial antioxidants status in experimental mammary carcinoma.</p><p><strong>Methods</strong>:<strong> </strong><em>Elaecoarpus ganitrus </em>(fruits), <em>Terminalia chebula </em>(seed coats), <em>Prosopis cineraria </em>(leaves)<em>, </em>adult female albino rats of Sprague-Dawley strain weighing 170–190 g and 7,12-dimethylbenzeneanthracene (DMBA) were used for this study. Group I control rats, Group II rats mammary carcinoma induced with DMBA (25 mg in 1 ml olive oil) by gastric intubation. Group III, IV and V DMBA induced rats were treated with TD (400 mg/kg. b. wt/day), PGG (30 mg/kg. b. wt/day) and standard drug, Cyclophosphamide (30 mg/kg. b. wt/day), respectively for 48 d by gastric intubation. Group VI and VII rats served as TD and PGG treated controls, respectively for 48 d by gastric intubation. At the end of the experimental period, the rats were anaesthetized and sacrificed. Mammary glands were isolated and used for biochemical assays and histopathological evaluation.</p><p><strong>Results: </strong>In rats with cancer, the lipid peroxide levels (LPO) were significantly increased and mitochondrial antioxidant levels were decreased. Treatment with TD and PGG decreased LPO levels and increased mitochondrial antioxidant status in mammary carcinoma bearing rats. Histopathological analysis also confirmed the therapeutic effect of TD and PGG. No significant adverse effect was observed in sole drug treated group of rats.</p><p><strong>Conclusion: </strong>TD and PGG have definite therapeutic effect in experimental mammary carcinoma and inhibit growth of cancer cells by restoring mitochondrial antioxidant status and energy metabolism to normal states.</p>

scholarly journals The Effect of Parenteral Infusion of Amino Acids in Burn Patient

2013 ◽  
Vol 24 (1) ◽  
pp. 12-15
Author(s):  
Kamrun Nahar ◽  
Zeba-un Naher ◽  
Matira Khanam ◽  
Shaheen Akhter ◽  
Tahmina Bashar ◽  
...  
Keyword(s):  
Amino Acid ◽  
Serum Albumin ◽  
Total Protein ◽  
Burn Injury ◽  
Burn Patients ◽  
Serum Total Protein ◽  
Female Ratio ◽  
Group I ◽  
The Mean ◽  
Group Ii

Adequate nutritional support may prevent weight loss  following severe burn injury. However, persistently low  levels of serum albumin, transferring and serum total  protein in burn patients have suggested that a protein  deficiency may continue to exist which is out of proportion  to energy requirements.  This interventional study cross sectional study was done in  the Department of Biochemistry, Bangabandhu Sheikh  Mujib Medical University (BSMMU), Dhaka, Bangladesh  during January 2008 to December 2008. A total of 40 acute  burn injury (within 24 hours of burn) patients of 20-45  years age with 15%-30% burn were selected for this study  as case. The study subjects were divided into two groups:  Group I represent superficial burn & Group II represents  deep burn.  The mean age of 28.35±6.81 years and 30.85±7.32 years in  group I and group II respectively. The number of male in  Group-I was 08 and Group-II was 08 and male female ratio  was 2:3. The mean serum total protein before infusion of  amino acid in Group-I was 55.31±3.58 g/L and in Group-II  was 52.01±2.26 g/L (p<0.001). The mean serum total  protein after infusion of amino acid in Group-I was  68.02±2.04 g/L and in Group-II was 61.86±2.49g/L  (p<0.001). The mean serum albumin before infusion of  amino acid in Group-I was 27.6±2.88 g/L and in Group-II  was 25.57±1.89 g/L (p<0.001). The mean serum albumin  after infusion of amino acid in Group-I was 22.29±3.50 g/L  and in Group-II was 19.83±2.86 g/L (p<0.001). In group-I,  serum total protein was increased by 22.98% after infusion  and in group-II, that was increased by 18.94% (p<0.01).  In group-I, serum albumin was decreased by 19.24% after  infusion and in group-II, that was decreased by 22.45%  (p<0.05). Serum total protein significantly increased after  infusion of amino acid but serum albumin significantly  decreased after infusion of amino acid. DOI: http://dx.doi.org/10.3329/medtoday.v24i1.14107 Medicine TODAY Vol.24(1) 2012 pp.12-15

scholarly journals The effect of camel milk curd masses on rats blood serum biochemical parameters: Preliminary study

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256661
Author(s):  
Fatima Dikhanbayeva ◽  
Elmira Zhaxybayeva ◽  
Zhuldyz Smailova ◽  
Arman Issimov ◽  
Zhechko Dimitrov ◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Camel Milk ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Group V ◽  
Group I ◽  
Serum Biochemical ◽  
Group Ii

This study aimed to assess potential feeding effect of camel milk curd mass and its mixes to experimental rat’s blood serum biochemical parameters, enzymatic activity and the peptide toxicity. Fifty healthy male Sprague-Dawley rats were divided into five groups (n = 10 each). Each group was fed with camel milk pure curd mass and its mixes for 16 days. At the end of the experiment, rats were sacrificed to collect the samples from the blood serum. Blood serum biochemical parameters total protein, cholesterol, glucose, albumin, triglycerides; the enzymatic activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase were determined on the A25 automatic analyser, and peptide toxicity analysed by the reference method. The statistical data have shown no significant differences in body weight gain in all groups. Total protein decreased in group II, IV, and V; however, it increased in group III compared to the control group. Cholesterol grew up in group II and it slightly increased in group V, dropped in groups III and IV compared to group I result. Glucose increased in groups II, III, IV compared to group I; still, group V results show a slight decrease. Albumin decreased in group IV, yet in group V it increased than the group I result. Simultaneously, groups II and III results were changed with less percentage. Triglyceride grew up in groups II, V, and it dropped significantly in groups III, IV compared to the control group. De Ritis ratio of enzymes in groups II, III, and IV fluctuated between 1.31 and 0.98 IU/L; however, group V demonstrated significant data versus group I. Diets peptide toxicity in all groups was lower than control group data. The experimental results indicated that curd mass from camel milk could be used as a pure or with additives and it did not discover the observed side effects.

scholarly journals Protective Effect of Terminalia Chebula Extract on Serum Total Protein against

2017 ◽  
Vol 46 (1) ◽  
pp. 11-14
Author(s):  
Tania Yeasmin ◽  
Kazi Shamima Akhter ◽  
Masud Imtiaz
Keyword(s):  
Protective Effect ◽  
Total Protein ◽  
Protein Level ◽  
Control Group ◽  
Albino Rats ◽  
Terminalia Chebula ◽  
Serum Total Protein ◽  
Medical College ◽  
Total Protein Level ◽  
Serum Total Protein Level

Terminalia Chebula extract is used for regeneration of hepatic cells and protection of liver against damage due to its active component. This study aims to observe the protective effect of Terminalia Chebula against Paracetamol induced change of serum total protein level in Wister Albino rats. The study was carried out in the Department of Physiology, Dhaka Medical College (DMC) during January 2013 to December 2013. A total number of 44 rats, age ranging from 90 to 120 days, weight between 150 to 200 gm (initial body weight) were selected for the study. After acclimatization for 14 days, they were divided into control groups and experimental groups. Before sacrifice, final body weights of all the rats were measured. then all the rats were sacrificed on 22nd day and then blood samples were collected. For assessment of liver function, serum total protein level was done by using standard laboratory kits. The mean serum total protein level was significantly (p<0.001) lower in paracetamol treated control group in comparison to those of baseline control group. Serum total protein level of all experimental groups were significantly (P<0.001) higher than Paracetamol treated control group. From the results of this study, it may be concluded that Terminalia Chebula may have some protective effect against Paracetamol induced liver damage in rats.Bangladesh Med J. 2017 Jan; 46 (1): 11-14

Haematological and biochemical toxicity induced by methanol in rats: Ameliorative effects of Opuntia vulgaris fruit extract

2011 ◽  
Vol 30 (12) ◽  
pp. 1963-1971 ◽  
Author(s):  
Mongi Saoudi ◽  
Samira Jebahi ◽  
Kamel Jamoussi ◽  
Ghada Ben Salah ◽  
Choumous Kallel ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Total Protein ◽  
Natural Antioxidants ◽  
Treated Group ◽  
Protective Effects ◽  
Fruit Extract ◽  
Serum Total Protein ◽  
Potential Source ◽  
Antioxidant Enzymes Activities

The ameliorative effects of Opuntia vulgaris fruit extract (OE) were evaluated against methanol-induced haematological and biochemical toxicity in rats. The methanol-induced haematological and biochemical perturbation significantly decreased the levels of red blood cell (RBC), haemoglobin (Hb), haematocrit (Ht), serum total protein and increased glucose, cholesterol, and triglyceride levels in serum. Treatment of rats with methanol significantly increased lipid peroxidation (LPO) level and decreased the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in erythrocytes. OE treatment could increase significantly the levels of RBC, Hb, Ht and total protein, and decrease glucose, cholesterol and triglyceride levels in serum, and increase the activities of SOD, CAT and GPx in erythrocytes, when compared with methanol-treated group. Spleen histopathology showed that OE could significantly reduce the incidence of spleen lesion induced by methanol. These results suggested that OE could exhibit a potential source of natural antioxidants against methanol-induced haematological and biochemical disruption in rats. The protective effects of OE may be due to the modulation of antioxidant enzymes activities and inhibition of LPO.

scholarly journals Comparison of Serum Total Protein and Serum Albumin after Infusion of Amino Acid of the 15%-30% Burn Patients

2013 ◽  
Vol 9 (1-2) ◽  
pp. 109-113
Author(s):  
N Kamrun ◽  
Zeba Un-Naher ◽  
M Ali ◽  
T Bashar ◽  
MI Arslan
Keyword(s):  
Amino Acid ◽  
Serum Albumin ◽  
Total Protein ◽  
Burn Injury ◽  
Burn Patients ◽  
Amino Acid Supplementation ◽  
Serum Total Protein ◽  
Cross Sectional ◽  
Group I ◽  
Group Ii

The aim of the study to find out the ratio of raise of serum total protein and serum albumin during amino acid supplementation in burn patients. This interventional study cross sectional study was done in the Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during January 2008 to December 2008. A total of 40 acute burn injury (within 24 hours of burn) patients of 20-45 years age with 15%-30% burn were selected for this study as case. The study subjects were divided into two groups: Group I represent superficial burn & Group II represents deep burn. In group-I, serum albumin was decreased by 19.24% after infusion and in group-II, that was decreased by 22.45% (p<0.05). Serum total protein significantly increased after infusion of amino acid but serum albumin significantly decreased after infusion of amino acid. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14653 J. Sci. Foundation, 9(1&2): 109-113, June-December 2011

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